BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00011069
         (1214 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P32063.1|STAD_CORSA RecName: Full=Omega-12 acyl-[acyl-carrier...   612   e-173
gb|AAY46941.1| plastid delta4 multifunctional acyl-acyl carrier ...   535   e-149
pdb|2UW1|B Chain B, Ivy Desaturase Structure                          523   e-146
pdb|2UW1|A Chain A, Ivy Desaturase Structure                          522   e-146
gb|ADE06393.1| stearoyl-acyl-carrier protein desaturase [Macadam...   486   e-135

>sp|P32063.1|STAD_CORSA RecName: Full=Omega-12 acyl-[acyl-carrier-protein] desaturase,
            chloroplastic; AltName: Full=Stearoyl-ACP desaturase;
            Flags: Precursor gi|167389|gb|AAC63059.1|
            delta-4-palmitoyl-acyl carrier protein desaturase
            [Coriandrum sativum] gi|59896210|gb|AAX11452.1|
            delta-4-palmitoyl-ACP desaturase [Coriandrum sativum]
          Length = 385

 Score =  612 bits (1577), Expect = e-173
 Identities = 306/363 (84%), Positives = 325/363 (89%)
 Frame = -2

Query: 1090 QCPKTKSFSGTVTPKVGRLRSSSKVFMASTLHADHKPLVFDDHKLRAAKPELDEMFKSLD 911
            QCPK   F+    P+ GR+RS  KV MASTLHA   PLVFD  KL+A +PE+DE+F SL+
Sbjct: 12   QCPKRNMFTRIAPPQAGRVRS--KVSMASTLHAS--PLVFD--KLKAGRPEVDELFNSLE 65

Query: 910  GWARDNILVHLKSVENSWQPQDYLPDPTSDGFEEQVKEIRERAKEIPDDYFVVLVGDMIT 731
            GWARDNILVHLKSVENSWQPQDYLPDPTSD FE+QVKE+RERAK+IPD+YFVVLVGDMIT
Sbjct: 66   GWARDNILVHLKSVENSWQPQDYLPDPTSDAFEDQVKEMRERAKDIPDEYFVVLVGDMIT 125

Query: 730  EEALPTYMSMLNRCDGIKDESGXXXXXXXXXXXXXXXXENRHGDLLNKYLYLSGRVDMRM 551
            EEALPTYMSMLNRCDGIKD++G                ENRHGDLLNKYLYLSGRVDMRM
Sbjct: 126  EEALPTYMSMLNRCDGIKDDTGAQPTSWATWTRAWTAEENRHGDLLNKYLYLSGRVDMRM 185

Query: 550  IEKTIQYLIGSGMDVKTENCPYMGFIYTSFQERATFISHANTAKLAQHYGDKNLAQVCGN 371
            IEKTIQYLIGSGMD KTENCPYMGFIYTSFQERATFISHANTAKLAQHYGDKNLAQVCGN
Sbjct: 186  IEKTIQYLIGSGMDTKTENCPYMGFIYTSFQERATFISHANTAKLAQHYGDKNLAQVCGN 245

Query: 370  IASDEKRHATAYTKIVEKLAEIDPDTTVIAFADMMRKKIQMPAHAMYDGSDNMLFKHFTA 191
            IASDEKRHATAYTKIVEKLAEIDPDTTVIAF+DMMRKKIQMPAHAMYDGSD+MLFKHFTA
Sbjct: 246  IASDEKRHATAYTKIVEKLAEIDPDTTVIAFSDMMRKKIQMPAHAMYDGSDDMLFKHFTA 305

Query: 190  VAQQIGVYSAWDYCDIIDFLVEKWSVEKMTGLSGEGRKAQEYVCGLAARIRRVEEKVQGK 11
            VAQQIGVYSAWDYCDIIDFLV+KW+V KMTGLSGEGRKAQEYVC LAA+IRRVEEKVQGK
Sbjct: 306  VAQQIGVYSAWDYCDIIDFLVDKWNVAKMTGLSGEGRKAQEYVCSLAAKIRRVEEKVQGK 365

Query: 10   EKK 2
            EKK
Sbjct: 366  EKK 368


>gb|AAY46941.1| plastid delta4 multifunctional acyl-acyl carrier protein desaturase
            [Hedera helix]
          Length = 361

 Score =  535 bits (1377), Expect = e-149
 Identities = 266/344 (77%), Positives = 296/344 (86%), Gaps = 7/344 (2%)
 Frame = -2

Query: 1012 MASTLHADHKPLVFDDHK-------LRAAKPELDEMFKSLDGWARDNILVHLKSVENSWQ 854
            MAST++++   +V D+ K         +  P+  E+FKSLD WAR+N+L+HLKSVE SWQ
Sbjct: 1    MASTVNSNS--MVLDNLKSPPNLQVTHSMPPQKLEIFKSLDDWARNNVLIHLKSVEKSWQ 58

Query: 853  PQDYLPDPTSDGFEEQVKEIRERAKEIPDDYFVVLVGDMITEEALPTYMSMLNRCDGIKD 674
            PQDYLPDP SDGFEEQV+E+RERAKEIPDDYFVVLVGDMITEEALPTYMSMLNRCDGIKD
Sbjct: 59   PQDYLPDPVSDGFEEQVRELRERAKEIPDDYFVVLVGDMITEEALPTYMSMLNRCDGIKD 118

Query: 673  ESGXXXXXXXXXXXXXXXXENRHGDLLNKYLYLSGRVDMRMIEKTIQYLIGSGMDVKTEN 494
            E+G                ENRHGDLLNKYLYLSGRVDMR IEKTIQYLIGSGMD+K+EN
Sbjct: 119  ETGAEPSAWAMWTRAWTAEENRHGDLLNKYLYLSGRVDMRKIEKTIQYLIGSGMDIKSEN 178

Query: 493  CPYMGFIYTSFQERATFISHANTAKLAQHYGDKNLAQVCGNIASDEKRHATAYTKIVEKL 314
             PY+GFIYTSFQERATFISHANTAKLAQHYGDKNLA +CG+IASDEKRHATAYTKIVEKL
Sbjct: 179  SPYLGFIYTSFQERATFISHANTAKLAQHYGDKNLAHICGSIASDEKRHATAYTKIVEKL 238

Query: 313  AEIDPDTTVIAFADMMRKKIQMPAHAMYDGSDNMLFKHFTAVAQQIGVYSAWDYCDIIDF 134
            AEIDPDTTVIAFADMMRKKI MPAH MYDGSD +LFKHFTAVAQ++GVYSA DYCDI++F
Sbjct: 239  AEIDPDTTVIAFADMMRKKITMPAHLMYDGSDELLFKHFTAVAQRVGVYSALDYCDILEF 298

Query: 133  LVEKWSVEKMTGLSGEGRKAQEYVCGLAARIRRVEEKVQGKEKK 2
            LV+KW+VE++TGLS EGRKAQEYVC L  +IRRVEEKVQGKEKK
Sbjct: 299  LVDKWNVERLTGLSDEGRKAQEYVCELGPKIRRVEEKVQGKEKK 342


>pdb|2UW1|B Chain B, Ivy Desaturase Structure
          Length = 338

 Score =  523 bits (1346), Expect = e-146
 Identities = 252/314 (80%), Positives = 280/314 (89%)
 Frame = -2

Query: 943 PELDEMFKSLDGWARDNILVHLKSVENSWQPQDYLPDPTSDGFEEQVKEIRERAKEIPDD 764
           P+  E+FKSLD WAR+N+L+HLKSVE SWQPQDYLPDP SDGFEEQV+E+RERAKEIPDD
Sbjct: 9   PQKLEIFKSLDDWARNNVLIHLKSVEKSWQPQDYLPDPVSDGFEEQVRELRERAKEIPDD 68

Query: 763 YFVVLVGDMITEEALPTYMSMLNRCDGIKDESGXXXXXXXXXXXXXXXXENRHGDLLNKY 584
           YFVVLVGDMITEEALPTYMSMLNRCDGIKDE+G                ENRHGDLLNKY
Sbjct: 69  YFVVLVGDMITEEALPTYMSMLNRCDGIKDETGAEPSAWAMWTRAWTAEENRHGDLLNKY 128

Query: 583 LYLSGRVDMRMIEKTIQYLIGSGMDVKTENCPYMGFIYTSFQERATFISHANTAKLAQHY 404
           LYLSGRVDMR IEKTIQYLIGSGMD+K+EN PY+GFIYTSFQERATFISHANTAKLAQH+
Sbjct: 129 LYLSGRVDMRKIEKTIQYLIGSGMDIKSENSPYLGFIYTSFQERATFISHANTAKLAQHW 188

Query: 403 GDKNLAQVCGNIASDEKRHATAYTKIVEKLAEIDPDTTVIAFADMMRKKIQMPAHAMYDG 224
           GDKNLA +CG+IASDEKRHATAYTKIVEKLAEIDPDTTVIAFADMMRKKI MPAH MYDG
Sbjct: 189 GDKNLAHICGSIASDEKRHATAYTKIVEKLAEIDPDTTVIAFADMMRKKITMPAHLMYDG 248

Query: 223 SDNMLFKHFTAVAQQIGVYSAWDYCDIIDFLVEKWSVEKMTGLSGEGRKAQEYVCGLAAR 44
           SD +LFKHFTAVAQ++GVYSA DYCDI++FLV+KW+VE++TGLS EGRKAQEYVC L  +
Sbjct: 249 SDELLFKHFTAVAQRVGVYSALDYCDILEFLVDKWNVERLTGLSDEGRKAQEYVCELGPK 308

Query: 43  IRRVEEKVQGKEKK 2
           IRR+EE+ QG+ K+
Sbjct: 309 IRRLEERAQGRAKE 322


>pdb|2UW1|A Chain A, Ivy Desaturase Structure
          Length = 338

 Score =  522 bits (1344), Expect = e-146
 Identities = 252/314 (80%), Positives = 279/314 (88%)
 Frame = -2

Query: 943 PELDEMFKSLDGWARDNILVHLKSVENSWQPQDYLPDPTSDGFEEQVKEIRERAKEIPDD 764
           P+  E+FKSLD WAR+N+L+HLKSVE SWQPQDYLPDP SDGFEEQV+E+RERAKEIPDD
Sbjct: 9   PQKLEIFKSLDDWARNNVLIHLKSVEKSWQPQDYLPDPVSDGFEEQVRELRERAKEIPDD 68

Query: 763 YFVVLVGDMITEEALPTYMSMLNRCDGIKDESGXXXXXXXXXXXXXXXXENRHGDLLNKY 584
           YFVVLVGDMITEEALPTYMSMLNRCDGIKDE+G                ENRHGDLLNKY
Sbjct: 69  YFVVLVGDMITEEALPTYMSMLNRCDGIKDETGAEPSAWAMWTRAWTAEENRHGDLLNKY 128

Query: 583 LYLSGRVDMRMIEKTIQYLIGSGMDVKTENCPYMGFIYTSFQERATFISHANTAKLAQHY 404
           LYLSGRVDMR IEKTIQYLIGSGMD+K+EN PY+GFIYTSFQERATFISHANTAKLAQHY
Sbjct: 129 LYLSGRVDMRKIEKTIQYLIGSGMDIKSENSPYLGFIYTSFQERATFISHANTAKLAQHY 188

Query: 403 GDKNLAQVCGNIASDEKRHATAYTKIVEKLAEIDPDTTVIAFADMMRKKIQMPAHAMYDG 224
           GDK LA +CG+IASDEKRHATAYTKIVEKLAEIDPDTTVIAFADMMRKKI MPAH MYDG
Sbjct: 189 GDKKLAHICGSIASDEKRHATAYTKIVEKLAEIDPDTTVIAFADMMRKKITMPAHLMYDG 248

Query: 223 SDNMLFKHFTAVAQQIGVYSAWDYCDIIDFLVEKWSVEKMTGLSGEGRKAQEYVCGLAAR 44
           SD +LFKHFTAVAQ++GVYSA DYCDI++FLV+KW+VE++TGLS EGRKAQEYVC L  +
Sbjct: 249 SDELLFKHFTAVAQRLGVYSALDYCDILEFLVDKWNVERLTGLSDEGRKAQEYVCELGPK 308

Query: 43  IRRVEEKVQGKEKK 2
           IRR+EE+ QG+ K+
Sbjct: 309 IRRLEERAQGRAKE 322


>gb|ADE06393.1| stearoyl-acyl-carrier protein desaturase [Macadamia tetraphylla]
          Length = 396

 Score =  486 bits (1251), Expect = e-135
 Identities = 247/375 (65%), Positives = 288/375 (76%), Gaps = 11/375 (2%)
 Frame = -2

Query: 1093 FQCPKTKSFSGTVTPKVGRLRSSSKVFMASTLHADHK-----------PLVFDDHKLRAA 947
            FQ  K  SF     P    LRS  KVFMASTL +  K           P         + 
Sbjct: 10   FQSQKYPSFG---LPTAANLRSP-KVFMASTLRSSSKEVENLKRPFSPPREVHVQVTHSM 65

Query: 946  KPELDEMFKSLDGWARDNILVHLKSVENSWQPQDYLPDPTSDGFEEQVKEIRERAKEIPD 767
             P+  E+FKSLD WA  NIL+HLKSVE SWQPQD+LPDP S+GF+EQVKE+RERAKEIPD
Sbjct: 66   PPQKIEIFKSLDDWAEKNILMHLKSVEKSWQPQDFLPDPASEGFDEQVKELRERAKEIPD 125

Query: 766  DYFVVLVGDMITEEALPTYMSMLNRCDGIKDESGXXXXXXXXXXXXXXXXENRHGDLLNK 587
            DYF+VLVGDMITEEALPTY +MLN  DG++DE+G                ENRHGDLLNK
Sbjct: 126  DYFIVLVGDMITEEALPTYQTMLNTLDGVRDETGASLTSWAVWTRAWTAEENRHGDLLNK 185

Query: 586  YLYLSGRVDMRMIEKTIQYLIGSGMDVKTENCPYMGFIYTSFQERATFISHANTAKLAQH 407
            YLYLSGRVDM+ IEKTIQYLIGSGMD +TEN PY+GFIYTSFQERATFISH NTA+LA+ 
Sbjct: 186  YLYLSGRVDMKQIEKTIQYLIGSGMDPRTENSPYLGFIYTSFQERATFISHGNTARLAKE 245

Query: 406  YGDKNLAQVCGNIASDEKRHATAYTKIVEKLAEIDPDTTVIAFADMMRKKIQMPAHAMYD 227
            +GD  LAQ+CG IA+DEKRH TAYTKIVEKL EIDPD TV+AFADMMRKKI MPAH MYD
Sbjct: 246  HGDMKLAQICGTIAADEKRHETAYTKIVEKLFEIDPDGTVLAFADMMRKKISMPAHLMYD 305

Query: 226  GSDNMLFKHFTAVAQQIGVYSAWDYCDIIDFLVEKWSVEKMTGLSGEGRKAQEYVCGLAA 47
            G D+ LF+HF+AVAQ++GVY+A DY DI++FLV +W+VEK+TGLSGEG+KAQ++VCGLA 
Sbjct: 306  GQDDNLFEHFSAVAQRLGVYTARDYADILEFLVGRWNVEKLTGLSGEGQKAQDFVCGLAP 365

Query: 46   RIRRVEEKVQGKEKK 2
            RIRR+EE+ QG+ K+
Sbjct: 366  RIRRLEERAQGRTKQ 380