BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00011032
         (1335 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACN78662.1| endo-beta-mannanase [Actinidia arguta]                 305   e-130
gb|ABV32548.1| endo-1,4-beta-mannosidase protein 2 [Prunus persica]   294   e-129
gb|ABY58035.1| mannase [Prunus salicina]                              290   e-127
ref|XP_002281804.1| PREDICTED: mannan endo-1,4-beta-mannosidase ...   290   e-123
ref|XP_004143522.1| PREDICTED: mannan endo-1,4-beta-mannosidase ...   292   e-121

>gb|ACN78662.1| endo-beta-mannanase [Actinidia arguta]
          Length = 433

 Score =  305 bits (780), Expect(2) = e-130
 Identities = 140/183 (76%), Positives = 167/183 (91%)
 Frame = +2

Query: 89  KVSAAFKEASAHGLTVARTWAFGDGGDRPLQLSPGSYNNHMFEGLDFVVAEARRYGVRLI 268
           KVSAAF+EAS HGL+VARTWAF DGG RPLQ SPG YN   F+GLDFVVAEARRYG++L+
Sbjct: 66  KVSAAFREASNHGLSVARTWAFSDGGYRPLQFSPGYYNEQAFKGLDFVVAEARRYGIKLV 125

Query: 269 LSLVDNYETFGGKKQYVKWARSQGEVVTSDDDFFKNTVVKEFYRSHVKTVLNRYNTITGV 448
           LSLV+NYE+FGGKKQYV WARSQG+ ++SDDDFF+N +VK +Y++HVKTVLNRYNT+TGV
Sbjct: 126 LSLVNNYESFGGKKQYVNWARSQGQYLSSDDDFFRNPLVKGYYKNHVKTVLNRYNTMTGV 185

Query: 449 VYKSDPTIMAWELMNEARCSSDSSGHTIQAWIIEMAAHVKSIDKNHMLEAGLEGFYGETT 628
           +YK+DPTIMAWELMNE RC+SD SG TIQAWI+EMA++VKSID+NH+LEAGLEGFYG+TT
Sbjct: 186 IYKNDPTIMAWELMNEPRCTSDPSGRTIQAWIMEMASYVKSIDRNHLLEAGLEGFYGQTT 245

Query: 629 AQK 637
            Q+
Sbjct: 246 PQR 248



 Score =  187 bits (474), Expect(2) = e-130
 Identities = 96/181 (53%), Positives = 125/181 (69%), Gaps = 6/181 (3%)
 Frame = +1

Query: 757  PIYSTGTDFITNNQTPDIDFATTHSYPDKWLSDFSEEKQLDFMNNWLDIHIHDAQYVLQK 936
            P ++ GTDFI NN+ P IDFAT HSYPD+WL+  +E+ QL F+NNWLD HI DAQ+VL+K
Sbjct: 253  PGFNIGTDFIANNRVPWIDFATVHSYPDQWLTSSNEQNQLSFLNNWLDTHIQDAQFVLRK 312

Query: 937  PLLITEFGKYLKDSGISSSQRDNLFKIVYQKIYXXXXXXXXXXXXLFWQLLSEDMDKFKD 1116
            PLL+TEFGK  KD G S+ QRD LF  VY KIY            LFWQLL+E MD F+D
Sbjct: 313  PLLLTEFGKSWKDPGYSTYQRDLLFNTVYYKIYSSAKRGGAAAGGLFWQLLTEGMDSFRD 372

Query: 1117 GYEIVLSQSPSTADMISQQCHKL---STIFSKG*H*AMEKGKSCYVAK---WLARNKGMQ 1278
            GY+IVLS+SPSTA++I+QQ H+L     IF++  +  +E+ K    A+   W +RN+G  
Sbjct: 373  GYDIVLSESPSTANVIAQQSHRLYQIRKIFARMRN--VERWKRARWARRNPWFSRNRGKP 430

Query: 1279 V 1281
            +
Sbjct: 431  I 431


>gb|ABV32548.1| endo-1,4-beta-mannosidase protein 2 [Prunus persica]
          Length = 433

 Score =  294 bits (753), Expect(2) = e-129
 Identities = 137/195 (70%), Positives = 167/195 (85%), Gaps = 4/195 (2%)
 Frame = +2

Query: 65  VMHQGSGP----KVSAAFKEASAHGLTVARTWAFGDGGDRPLQLSPGSYNNHMFEGLDFV 232
           +M+  S P    KVS+ F+EA++HGLTVARTWAF DGG RPLQ SPGSYN  MF+GLDFV
Sbjct: 54  LMYVASDPSQRYKVSSVFREATSHGLTVARTWAFSDGGYRPLQYSPGSYNEQMFKGLDFV 113

Query: 233 VAEARRYGVRLILSLVDNYETFGGKKQYVKWARSQGEVVTSDDDFFKNTVVKEFYRSHVK 412
           +AEARRYG++LILSLV+NYE+FGG+KQYV WARSQG+ +TSDDDF++N VVK +Y++HV 
Sbjct: 114 IAEARRYGIKLILSLVNNYESFGGRKQYVNWARSQGQYLTSDDDFYRNPVVKGYYKNHVT 173

Query: 413 TVLNRYNTITGVVYKSDPTIMAWELMNEARCSSDSSGHTIQAWIIEMAAHVKSIDKNHML 592
           TVLNRYN+ T V YK DPTIMAWEL+NE RC+SD SG T+QAWI+EMA+HVKSID+NH+L
Sbjct: 174 TVLNRYNSYTRVHYKDDPTIMAWELINEPRCTSDPSGRTVQAWIMEMASHVKSIDRNHLL 233

Query: 593 EAGLEGFYGETTAQK 637
           EAGLEGFYG+   Q+
Sbjct: 234 EAGLEGFYGQAIPQR 248



 Score =  195 bits (495), Expect(2) = e-129
 Identities = 98/179 (54%), Positives = 126/179 (70%), Gaps = 4/179 (2%)
 Frame = +1

Query: 757  PIYSTGTDFITNNQTPDIDFATTHSYPDKWLSDFSEEKQLDFMNNWLDIHIHDAQYVLQK 936
            P ++ GTDFI NN+ P IDFAT HSYPD+WLS  +++ QL F+NNWLD HI DAQY+L+K
Sbjct: 253  PGFNIGTDFIANNRIPGIDFATVHSYPDQWLSSSNDQNQLSFLNNWLDTHIQDAQYILRK 312

Query: 937  PLLITEFGKYLKDSGISSSQRDNLFKIVYQKIYXXXXXXXXXXXXLFWQLLSEDMDKFKD 1116
            P+ ITEFGK  KD G ++ QRD LF  VY KIY            LFWQLL+E MD F D
Sbjct: 313  PVFITEFGKSWKDPGFNTYQRDLLFNTVYSKIYSSARRGGAAAGGLFWQLLTEGMDSFGD 372

Query: 1117 GYEIVLSQSPSTADMISQQCHKL---STIFSKG*H*AM-EKGKSCYVAKWLARNKGMQV 1281
            GY+IVLSQSPSTA++I+QQ HKL     IF++  +  M ++ ++   A+WLARNKG ++
Sbjct: 373  GYDIVLSQSPSTANVIAQQSHKLYQIRKIFARIRNAQMWKRARAIRRAEWLARNKGKRI 431


>gb|ABY58035.1| mannase [Prunus salicina]
          Length = 433

 Score =  290 bits (742), Expect(2) = e-127
 Identities = 134/195 (68%), Positives = 166/195 (85%), Gaps = 4/195 (2%)
 Frame = +2

Query: 65  VMHQGSGP----KVSAAFKEASAHGLTVARTWAFGDGGDRPLQLSPGSYNNHMFEGLDFV 232
           +M+  S P    KVS+ F+EA++HGLTVARTWAF DGG RPLQ SPGSYN  +F+GLDFV
Sbjct: 54  LMYVASDPSQRYKVSSVFREATSHGLTVARTWAFSDGGYRPLQYSPGSYNEQLFKGLDFV 113

Query: 233 VAEARRYGVRLILSLVDNYETFGGKKQYVKWARSQGEVVTSDDDFFKNTVVKEFYRSHVK 412
           +AEARRYG++LILSLV+NYE+FGG+KQYV W RS+G+ +TSDDDF++N VVK +Y++HV 
Sbjct: 114 IAEARRYGIKLILSLVNNYESFGGRKQYVNWTRSRGQYLTSDDDFYRNPVVKGYYKNHVT 173

Query: 413 TVLNRYNTITGVVYKSDPTIMAWELMNEARCSSDSSGHTIQAWIIEMAAHVKSIDKNHML 592
           TVLNRYN+ T V YK DPTIMAWEL+NE RC+SD SG T+QAWI+EMA+HVKSID+NH+L
Sbjct: 174 TVLNRYNSYTRVHYKDDPTIMAWELINEPRCTSDPSGRTVQAWIMEMASHVKSIDRNHLL 233

Query: 593 EAGLEGFYGETTAQK 637
           EAGLEGFYG+   Q+
Sbjct: 234 EAGLEGFYGQAIPQR 248



 Score =  192 bits (489), Expect(2) = e-127
 Identities = 97/179 (54%), Positives = 125/179 (69%), Gaps = 4/179 (2%)
 Frame = +1

Query: 757  PIYSTGTDFITNNQTPDIDFATTHSYPDKWLSDFSEEKQLDFMNNWLDIHIHDAQYVLQK 936
            P ++ GTDFI NN+ P IDFAT HSYPD+WLS  +++ QL FMNNWL+ HI DAQY+L+K
Sbjct: 253  PGFNIGTDFIANNRIPGIDFATVHSYPDQWLSSSNDQNQLSFMNNWLNTHIQDAQYILRK 312

Query: 937  PLLITEFGKYLKDSGISSSQRDNLFKIVYQKIYXXXXXXXXXXXXLFWQLLSEDMDKFKD 1116
            P+ ITEFGK  KD G ++ QRD  F  VY KIY            LFWQLL+E MD F D
Sbjct: 313  PVFITEFGKSWKDPGFNTYQRDLFFNTVYSKIYSSARRGGAAAGALFWQLLTEGMDSFGD 372

Query: 1117 GYEIVLSQSPSTADMISQQCHKL---STIFSKG*H*AM-EKGKSCYVAKWLARNKGMQV 1281
            GY+IVLSQSPSTA++I+QQ HKL     IF++  +  M ++ ++   A+WLARNKG ++
Sbjct: 373  GYDIVLSQSPSTANVIAQQSHKLYQIRKIFARIRNAQMWKRARAIRRAEWLARNKGKRI 431


>ref|XP_002281804.1| PREDICTED: mannan endo-1,4-beta-mannosidase 7 [Vitis vinifera]
           gi|302142526|emb|CBI19729.3| unnamed protein product
           [Vitis vinifera]
          Length = 433

 Score =  290 bits (742), Expect(2) = e-123
 Identities = 134/195 (68%), Positives = 165/195 (84%), Gaps = 4/195 (2%)
 Frame = +2

Query: 65  VMHQGSGP----KVSAAFKEASAHGLTVARTWAFGDGGDRPLQLSPGSYNNHMFEGLDFV 232
           +M+  S P    K+S+AF EA+ HGLT+ARTWAF DGG RPLQ SPGSYN  MF+GLDFV
Sbjct: 54  LMYVASDPSQRSKISSAFAEAAGHGLTIARTWAFSDGGYRPLQYSPGSYNEQMFQGLDFV 113

Query: 233 VAEARRYGVRLILSLVDNYETFGGKKQYVKWARSQGEVVTSDDDFFKNTVVKEFYRSHVK 412
           V+EA +YG++L+LSL +NYE+FGGKKQYV WAR QG+ +TSDDDFF+N+V K +Y++H+K
Sbjct: 114 VSEAGKYGIKLVLSLANNYESFGGKKQYVDWARGQGQYLTSDDDFFRNSVAKGYYKNHIK 173

Query: 413 TVLNRYNTITGVVYKSDPTIMAWELMNEARCSSDSSGHTIQAWIIEMAAHVKSIDKNHML 592
           TVL R N+ITGVVYK +PTIMAWELMNE RC+SD SG TIQAWI EMA++VKS+D NH+L
Sbjct: 174 TVLTRRNSITGVVYKDEPTIMAWELMNEPRCTSDPSGRTIQAWITEMASYVKSVDGNHLL 233

Query: 593 EAGLEGFYGETTAQK 637
           EAGLEGFYG+T+ QK
Sbjct: 234 EAGLEGFYGQTSPQK 248



 Score =  180 bits (456), Expect(2) = e-123
 Identities = 93/176 (52%), Positives = 113/176 (64%), Gaps = 4/176 (2%)
 Frame = +1

Query: 757  PIYSTGTDFITNNQTPDIDFATTHSYPDKWLSDFSEEKQLDFMNNWLDIHIHDAQYVLQK 936
            P +  GTDFI NNQ P IDFAT HSYPD+W S  S++ QL F+N WL  HI DAQ VL+K
Sbjct: 253  PNFQVGTDFIANNQIPGIDFATVHSYPDQWFSSSSDDTQLSFLNEWLKNHIQDAQNVLRK 312

Query: 937  PLLITEFGKYLKDSGISSSQRDNLFKIVYQKIYXXXXXXXXXXXXLFWQLLSEDMDKFKD 1116
            PLL TEFGK  KDSG S+ QRD LF  VY  IY            +FWQLL+E MD F+D
Sbjct: 313  PLLFTEFGKSSKDSGYSTYQRDMLFNTVYSAIYSSARSGGAAVGGMFWQLLTEGMDSFRD 372

Query: 1117 GYEIVLSQSPSTADMISQQCHKLSTIFSKG*H----*AMEKGKSCYVAKWLARNKG 1272
            GYEIVLS+SPSTA++I+QQ HKL  +             ++ ++   A+W  RNKG
Sbjct: 373  GYEIVLSESPSTANVIAQQSHKLYLLRKMYARLRNIQKWKRARAIRRAQWWNRNKG 428


>ref|XP_004143522.1| PREDICTED: mannan endo-1,4-beta-mannosidase 7-like [Cucumis
           sativus] gi|449521449|ref|XP_004167742.1| PREDICTED:
           mannan endo-1,4-beta-mannosidase 7-like [Cucumis
           sativus]
          Length = 418

 Score =  292 bits (748), Expect(2) = e-121
 Identities = 137/195 (70%), Positives = 165/195 (84%), Gaps = 4/195 (2%)
 Frame = +2

Query: 65  VMHQGSGP----KVSAAFKEASAHGLTVARTWAFGDGGDRPLQLSPGSYNNHMFEGLDFV 232
           +M+  S P    KVSA F+EAS+HGLTVARTWAF DGG RPLQ SPG+YN  MF+G+DFV
Sbjct: 48  LMYVASDPSQRSKVSAVFREASSHGLTVARTWAFSDGGYRPLQYSPGNYNEQMFQGMDFV 107

Query: 233 VAEARRYGVRLILSLVDNYETFGGKKQYVKWARSQGEVVTSDDDFFKNTVVKEFYRSHVK 412
           VAEARR+G++LILSLV+NYE FGGKKQYV WARS+G+ + SDDDFF+N VVK FY++H++
Sbjct: 108 VAEARRFGIKLILSLVNNYENFGGKKQYVNWARSKGQYLNSDDDFFRNPVVKGFYKNHIR 167

Query: 413 TVLNRYNTITGVVYKSDPTIMAWELMNEARCSSDSSGHTIQAWIIEMAAHVKSIDKNHML 592
           TVLNRYN  T + YK DPTIMAWELMNE RC+SD SG TIQAWI+EMA+ VKSID+NH+L
Sbjct: 168 TVLNRYNRFTTIHYKDDPTIMAWELMNEPRCTSDPSGRTIQAWIMEMASFVKSIDRNHLL 227

Query: 593 EAGLEGFYGETTAQK 637
           EAGLEGFYG ++ Q+
Sbjct: 228 EAGLEGFYGHSSPQR 242



 Score =  171 bits (434), Expect(2) = e-121
 Identities = 80/146 (54%), Positives = 105/146 (71%)
 Frame = +1

Query: 757  PIYSTGTDFITNNQTPDIDFATTHSYPDKWLSDFSEEKQLDFMNNWLDIHIHDAQYVLQK 936
            P ++ GTDFI NN+ P IDFAT HSYPD+WLS  +++ QL F+NNW+  HI DA+ +L+K
Sbjct: 247  PGFNIGTDFIANNRIPGIDFATLHSYPDQWLSSSNDQYQLSFLNNWISTHIQDAESILRK 306

Query: 937  PLLITEFGKYLKDSGISSSQRDNLFKIVYQKIYXXXXXXXXXXXXLFWQLLSEDMDKFKD 1116
            P+L+TEFGK  K  G S+++RD LF  VY KIY            LFWQLL+E MD F+D
Sbjct: 307  PILLTEFGKSWKVPGYSTNERDLLFNTVYSKIYASARRGGAAAGGLFWQLLTEGMDSFRD 366

Query: 1117 GYEIVLSQSPSTADMISQQCHKLSTI 1194
            GYEI+LSQ+PSTA++I+QQ  +L  I
Sbjct: 367  GYEIILSQNPSTANVITQQSRRLYQI 392