BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00011012
         (2696 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002280960.2| PREDICTED: DNA topoisomerase 1-like [Vitis v...  1278   0.0  
ref|XP_003551158.1| PREDICTED: DNA topoisomerase 1-like [Glycine...  1178   0.0  
ref|XP_003545557.1| PREDICTED: DNA topoisomerase 1-like [Glycine...  1177   0.0  
ref|XP_002528003.1| prokaryotic DNA topoisomerase, putative [Ric...  1167   0.0  
ref|XP_004164103.1| PREDICTED: DNA topoisomerase 1-like, partial...  1141   0.0  

>ref|XP_002280960.2| PREDICTED: DNA topoisomerase 1-like [Vitis vinifera]
          Length = 1185

 Score = 1278 bits (3306), Expect = 0.0
 Identities = 630/834 (75%), Positives = 727/834 (87%), Gaps = 1/834 (0%)
 Frame = +1

Query: 100  KQTPETSVKPKPYKQRVIKQLYPPTGKSVLVVESVTKAKVIQGYLGDMFEVLPSYGHVRD 279
            KQT  T  K K  +QR +K+LYP +GKSV+VVESVTKAKVIQGYLGDM+EVLPSYGHVRD
Sbjct: 354  KQTVHTMGKIKSLEQRPLKKLYPSSGKSVVVVESVTKAKVIQGYLGDMYEVLPSYGHVRD 413

Query: 280  LAARSGSVRPDDDFSMVWEVPSAAWTHLKSIKVALSGAKNLILASDPDREGEAIAWHIIE 459
            LA RSGSVRPDDDFSMVWEVPSAAWTHLKSIKVAL GA+NLILASDPDREGEAIAWHIIE
Sbjct: 414  LAGRSGSVRPDDDFSMVWEVPSAAWTHLKSIKVALGGAENLILASDPDREGEAIAWHIIE 473

Query: 460  MLVQQDALREDVNLARVVFNEITESSIKTALLAPRDINANLVNSYLARRALDYLIGFNIS 639
            ML+QQDAL +D+ +ARVVF+EITESSIK+AL APR+I+ NLV++YLARRALDYLIGFNIS
Sbjct: 474  MLLQQDALHKDLTVARVVFHEITESSIKSALDAPREIDVNLVHAYLARRALDYLIGFNIS 533

Query: 640  PVLWRKLPGCRSAGRVQSAALSLICDREMEIEGFKPQEYWTVDVQFKEK-GSSLPVDIYS 816
            P+LWRKLPGC+SAGRVQSAAL+LICDREMEI+ FKPQEYWTV+V+F  K GSS+    + 
Sbjct: 534  PLLWRKLPGCQSAGRVQSAALALICDREMEIDEFKPQEYWTVEVEFNRKQGSSMNSKFFP 593

Query: 817  IKSYLTHFQSEKLSKLSISSHANAVEIQGVIEASEFQVSNIKRSKMRKNPPTPYITSSLQ 996
              SYLTHF S+KL++ SISSH  A  I+  I + EF+V   KR+KMRKNPPTPYITS+LQ
Sbjct: 594  --SYLTHFDSKKLNQFSISSHTEAKAIEQEINSLEFKVIGSKRNKMRKNPPTPYITSTLQ 651

Query: 997  QDAANKLNFPATYTMRLAQKLYEGVQLPDGASAGLITYIRTDGLHISDEACKDICSLVRE 1176
            QDAANKL+F A YTM+LAQ+LYEGVQL DG +AGLITY+RTDGLH+SDEA KDI SLV E
Sbjct: 652  QDAANKLHFSAMYTMKLAQRLYEGVQLSDGKAAGLITYMRTDGLHVSDEAAKDIRSLVAE 711

Query: 1177 RYGESHASDGARKYFKKVKNAQEAHEAIRPTNIRRVPSSLSGVLDEDSLKLYTLIWSRTV 1356
            RYG + ASDG RKYFKKVKNAQEAHEAIRPT+I+R+PS L+GVLDEDSLKLYTLIWSRT+
Sbjct: 712  RYGSNLASDGVRKYFKKVKNAQEAHEAIRPTDIQRLPSMLAGVLDEDSLKLYTLIWSRTM 771

Query: 1357 ACQMEPAIFYQIQVDIGNADQSIIFRSSCSRIEFPGYQAVFEDVEARLIRNRDNEGNERS 1536
            ACQMEPA   QIQVDIGNA++S++FRS+CS +EF GYQAV++DVEA+ IR  +NEGNER 
Sbjct: 772  ACQMEPATIDQIQVDIGNANESVVFRSTCSGVEFFGYQAVYKDVEAKAIRVDENEGNERG 831

Query: 1537 DVSDVLSRMKSGDPLYLGKVDLKQHHTQPPPRFSEGSLVKTMEELGIGRPSTYASTMKVL 1716
            +V   LS +K+GDPLYL  ++L+QHHT PP R+SEG+LVK +EELGIGRPSTYA TMKVL
Sbjct: 832  EVFKALSSLKAGDPLYLSLLELEQHHTLPPSRYSEGALVKKLEELGIGRPSTYAITMKVL 891

Query: 1717 QDRKYITVKSRVLYPEFRGRMLSAFLSNHFSEVTDYSFTADMETELDNVSAGLTEWKGLL 1896
            QDR Y+TVK+RVLYPEFRGRM+SAFLS+HFSEVTDYSFTADMETELDNVSAGLTEWKGLL
Sbjct: 892  QDRNYVTVKNRVLYPEFRGRMVSAFLSHHFSEVTDYSFTADMETELDNVSAGLTEWKGLL 951

Query: 1897 KDYWTRFSKYCESTSNLHIHEVEKMLEKTFGDFLFTSLPGKSRTCPSCREGTLIFKVSRL 2076
            KDYWTRFS YC   SN+HIH+VEKMLEK FGDFLF+ LP KSRTCPSC EGTLIFKVSR 
Sbjct: 952  KDYWTRFSMYCNRVSNVHIHQVEKMLEKKFGDFLFSFLPDKSRTCPSCMEGTLIFKVSRF 1011

Query: 2077 GAGYFIGCDQHPRCKYIAKTIHGXXXXXISPDNQNVEEPKILGVKPGSDEKILLKNGPYG 2256
            G+GYFIGCDQHP+CKYIAK + G     ++  ++  EEPK LG+ PGS EKILLKNGPYG
Sbjct: 1012 GSGYFIGCDQHPKCKYIAKMLDGDDDEEVASQDKTFEEPKALGLSPGSSEKILLKNGPYG 1071

Query: 2257 FYVQLGVDRKGYLPKRASVSQVKDTSSITLEDALELLRYPVTLGEHPDDGHPVILKLAKV 2436
            FY+QLG DRKGYLPKRASVS +KD  SITLEDALELLRYPVTLG HP+D HPV+LKLAK 
Sbjct: 1072 FYLQLGEDRKGYLPKRASVSHIKDVGSITLEDALELLRYPVTLGNHPNDDHPVVLKLAKN 1131

Query: 2437 GFAIKHRRTVSPVPKNMKPEDISLDKAVQILLSKDAKRSGRPKNKPKHQEIDAI 2598
            GF+I+HRRT++PVPKN+KP DI+L+KA+++LL KD K+SGRPKNK K +  +A+
Sbjct: 1132 GFSIRHRRTIAPVPKNIKPNDITLEKALKLLLGKDVKQSGRPKNKKKQEVYEAM 1185


>ref|XP_003551158.1| PREDICTED: DNA topoisomerase 1-like [Glycine max]
          Length = 1070

 Score = 1178 bits (3047), Expect = 0.0
 Identities = 580/825 (70%), Positives = 688/825 (83%), Gaps = 1/825 (0%)
 Frame = +1

Query: 130  KPYKQRVIKQLYPPTGKSVLVVESVTKAKVIQGYLGDMFEVLPSYGHVRDLAARSGSVRP 309
            KP      K LYPPTGKSV+VVESVTKAKVI+ YLGDM+EVLPSYGHVRDLAARSGSVRP
Sbjct: 245  KPLWNFTYKPLYPPTGKSVVVVESVTKAKVIKRYLGDMYEVLPSYGHVRDLAARSGSVRP 304

Query: 310  DDDFSMVWEVPSAAWTHLKSIKVALSGAKNLILASDPDREGEAIAWHIIEMLVQQDALRE 489
            DDDFSMVWEVPS AWTHLKSIKVALSGA+NLILASDPDREGEAIAWHIIEML QQ AL +
Sbjct: 305  DDDFSMVWEVPSPAWTHLKSIKVALSGAENLILASDPDREGEAIAWHIIEMLQQQGALHD 364

Query: 490  DVNLARVVFNEITESSIKTALLAPRDINANLVNSYLARRALDYLIGFNISPVLWRKLPGC 669
            +++LARVVF+EITE SIK +L  PR+I+ NLV +YLARRALDYLIGF+ISP+LWRKLPGC
Sbjct: 365  NISLARVVFHEITEKSIKGSLQTPREIDINLVQAYLARRALDYLIGFSISPLLWRKLPGC 424

Query: 670  RSAGRVQSAALSLICDREMEIEGFKPQEYWTVDVQFKEKGSSLPVDIYSIKSYLTHFQSE 849
            +SAGRVQSAALSLICDREMEI+ FKP+EYW V+VQ K+K      ++ +  ++LTHF S+
Sbjct: 425  QSAGRVQSAALSLICDREMEIDEFKPKEYWIVEVQLKKKELRSNKNL-TFPAHLTHFDSK 483

Query: 850  KLSKLSISSHANAVEIQGVIEASEFQVSNIKRSKMRKNPPTPYITSSLQQDAANKLNFPA 1029
            KL++ SI+S   A +IQ  I +++F V ++K+SK R+NPPTPYITS+LQQDAANKL+F A
Sbjct: 484  KLNQFSITSDTEARDIQSTINSADFHVVSLKKSKSRRNPPTPYITSTLQQDAANKLHFTA 543

Query: 1030 TYTMRLAQKLYEGVQLPDGASAGLITYIRTDGLHISDEACKDICSLVRERYGESHASDGA 1209
            ++TM+LAQKLYEGV+L DG + GLITYIRTDGLHISDE    I SL+ ERYG+   S  A
Sbjct: 544  SHTMKLAQKLYEGVELSDGIAVGLITYIRTDGLHISDEVVASIRSLIIERYGQDFVSQSA 603

Query: 1210 RKYFKKVKNAQEAHEAIRPTNIRRVPSSLSGVLDEDSLKLYTLIWSRTVACQMEPAIFYQ 1389
             KY+KKVKNAQEAHEAIRPT+I ++PS LSG+LD +SLKLYTLIWSRTV+CQM PA+F Q
Sbjct: 604  PKYYKKVKNAQEAHEAIRPTDICKLPSMLSGILDGESLKLYTLIWSRTVSCQMGPAVFEQ 663

Query: 1390 IQVDIGNADQSIIFRSSCSRIEFPGYQAVFEDVEARLIRNRDNEGNERSDVSDVLSRMKS 1569
            IQ++IGN D+SII RSS SR+EFPGY+AVF D+    ++++D++   R     VL+ +K+
Sbjct: 664  IQLNIGNPDKSIILRSSSSRVEFPGYRAVFTDIVTEAVQDKDSDEFSRDQTFGVLNSLKT 723

Query: 1570 GDPLYLGKVDLKQHHTQPPPRFSEGSLVKTMEELGIGRPSTYASTMKVLQDRKYITVKSR 1749
            GDPL++ + +  QHHTQPPPR+SE SLVK +EELGIGRPSTYAST+KVLQDR Y+TVKSR
Sbjct: 724  GDPLHVVQTEPNQHHTQPPPRYSEASLVKKLEELGIGRPSTYASTIKVLQDRNYVTVKSR 783

Query: 1750 VLYPEFRGRMLSAFLSNHFSEVTDYSFTADMETELDNVSAGLTEWKGLLKDYWTRFSKYC 1929
            VL PEFRG M+SAFLS+HFSEV DY FTADMETELDNVS GLTEWKGLL+DYWTRF  YC
Sbjct: 784  VLSPEFRGCMVSAFLSHHFSEVMDYGFTADMETELDNVSGGLTEWKGLLRDYWTRFKSYC 843

Query: 1930 ESTSNLHIHEVEKMLEKTFGDFLFTSLPGKSRTCPSCREGTLIFKVSRLGAGYFIGCDQH 2109
            E TSN+HIH+VEKMLEK FGD+LF SLP +SR CPSC EGTLIFKVSR GAGYFIGCDQH
Sbjct: 844  ERTSNVHIHQVEKMLEKKFGDYLFASLPDQSRVCPSCMEGTLIFKVSRFGAGYFIGCDQH 903

Query: 2110 PRCKYIAKTIHG-XXXXXISPDNQNVEEPKILGVKPGSDEKILLKNGPYGFYVQLGVDRK 2286
            PRCKYIAKT++G       S  N  VEEPK+LGV  GS EK+LLK+GPYG YVQLG DRK
Sbjct: 904  PRCKYIAKTLYGTEEEEDASQPNSRVEEPKLLGVTSGSYEKVLLKSGPYGVYVQLGEDRK 963

Query: 2287 GYLPKRASVSQVKDTSSITLEDALELLRYPVTLGEHPDDGHPVILKLAKVGFAIKHRRTV 2466
            GY+PKRASVS +KD  SITL+DALE+L+YP+TLG HP DG PVILKLA+ G  ++HRRTV
Sbjct: 964  GYIPKRASVSHIKDVDSITLKDALEVLQYPLTLGNHPKDGQPVILKLARAGLCVRHRRTV 1023

Query: 2467 SPVPKNMKPEDISLDKAVQILLSKDAKRSGRPKNKPKHQEIDAIE 2601
            + VPKN+KP D++L+KA++ L   D +RSGRPK K K QE++ IE
Sbjct: 1024 ASVPKNVKPSDVTLEKALEYLSGDDVRRSGRPKGKSKVQEVEVIE 1068


>ref|XP_003545557.1| PREDICTED: DNA topoisomerase 1-like [Glycine max]
          Length = 1197

 Score = 1177 bits (3045), Expect = 0.0
 Identities = 576/825 (69%), Positives = 690/825 (83%), Gaps = 1/825 (0%)
 Frame = +1

Query: 130  KPYKQRVIKQLYPPTGKSVLVVESVTKAKVIQGYLGDMFEVLPSYGHVRDLAARSGSVRP 309
            KP      K LYPPT KSV+VVESVTKAKVI+ YLGDM+EVLPSYGHVRDLAARSGSVRP
Sbjct: 372  KPPWNFTYKPLYPPTRKSVVVVESVTKAKVIKRYLGDMYEVLPSYGHVRDLAARSGSVRP 431

Query: 310  DDDFSMVWEVPSAAWTHLKSIKVALSGAKNLILASDPDREGEAIAWHIIEMLVQQDALRE 489
            DDDFSMVWEVP  AWTHLKSIKVALS A+NLILASDPDREGEAIAWHIIEML QQ AL +
Sbjct: 432  DDDFSMVWEVPPPAWTHLKSIKVALSRAENLILASDPDREGEAIAWHIIEMLQQQGALHD 491

Query: 490  DVNLARVVFNEITESSIKTALLAPRDINANLVNSYLARRALDYLIGFNISPVLWRKLPGC 669
            +++LARVVF+EITE SIK AL  PR+I+ NLV +YLARRALDYLIGFNISP+LWRKLPGC
Sbjct: 492  NISLARVVFHEITEKSIKGALQTPREIDVNLVQAYLARRALDYLIGFNISPLLWRKLPGC 551

Query: 670  RSAGRVQSAALSLICDREMEIEGFKPQEYWTVDVQFKEKGSSLPVDIYSIKSYLTHFQSE 849
            +SAGRVQSAALSLICDREMEI+ FKP+EYWTV+VQ K+K      ++ +  ++LTHF S+
Sbjct: 552  QSAGRVQSAALSLICDREMEIDEFKPKEYWTVEVQMKKKELRSNKNL-TFPAHLTHFDSK 610

Query: 850  KLSKLSISSHANAVEIQGVIEASEFQVSNIKRSKMRKNPPTPYITSSLQQDAANKLNFPA 1029
            KL+K SI+S   A +I+  I +++F V ++K++K R+NPPTPYITS+LQQDAANKL+F A
Sbjct: 611  KLNKFSITSDTEARDIRSNINSADFHVVSLKKNKSRRNPPTPYITSTLQQDAANKLHFTA 670

Query: 1030 TYTMRLAQKLYEGVQLPDGASAGLITYIRTDGLHISDEACKDICSLVRERYGESHASDGA 1209
            ++TM+LAQKLYEGV+L D  + GLITYIRTDGLHISDEA   I SL+ ERYG+   S  A
Sbjct: 671  SHTMKLAQKLYEGVELSDSVAVGLITYIRTDGLHISDEAVASIRSLIIERYGQDFVSQSA 730

Query: 1210 RKYFKKVKNAQEAHEAIRPTNIRRVPSSLSGVLDEDSLKLYTLIWSRTVACQMEPAIFYQ 1389
             KY+KKVKNAQEAHEAIRPT+IR++PS LS +LDE+SLKLYTLIWSRTV+CQMEPA+F Q
Sbjct: 731  PKYYKKVKNAQEAHEAIRPTDIRKLPSMLSAILDEESLKLYTLIWSRTVSCQMEPAVFEQ 790

Query: 1390 IQVDIGNADQSIIFRSSCSRIEFPGYQAVFEDVEARLIRNRDNEGNERSDVSDVLSRMKS 1569
            IQ+DIGN D+SII +SS SR+EFPGY+AVF D+ +  ++++ ++ +       VL+ +K+
Sbjct: 791  IQLDIGNLDESIILQSSISRVEFPGYRAVFTDIVSEAVQDKGSDESSHDQTFGVLNSLKT 850

Query: 1570 GDPLYLGKVDLKQHHTQPPPRFSEGSLVKTMEELGIGRPSTYASTMKVLQDRKYITVKSR 1749
            GD L++ + +  QHHTQPPPR+SE SLVK +EELGIGRPSTYAST+KVLQDR Y+TVKSR
Sbjct: 851  GDSLHVVQTEPNQHHTQPPPRYSEASLVKKLEELGIGRPSTYASTIKVLQDRNYVTVKSR 910

Query: 1750 VLYPEFRGRMLSAFLSNHFSEVTDYSFTADMETELDNVSAGLTEWKGLLKDYWTRFSKYC 1929
            VL PEFRGRM+SAFLSNHFSEVTDYSFTADMETELDNVSAGLTEWKGLL+DYWTRF  YC
Sbjct: 911  VLSPEFRGRMVSAFLSNHFSEVTDYSFTADMETELDNVSAGLTEWKGLLRDYWTRFKSYC 970

Query: 1930 ESTSNLHIHEVEKMLEKTFGDFLFTSLPGKSRTCPSCREGTLIFKVSRLGAGYFIGCDQH 2109
            E TSN+HIH+VEKMLEK FGD+LF SLP +SR CPSC EGTLIFKVSR GAGYFIGCDQH
Sbjct: 971  ERTSNVHIHQVEKMLEKKFGDYLFASLPDQSRVCPSCMEGTLIFKVSRFGAGYFIGCDQH 1030

Query: 2110 PRCKYIAKTIHG-XXXXXISPDNQNVEEPKILGVKPGSDEKILLKNGPYGFYVQLGVDRK 2286
            PRCKYIAKT++G       S  N  +EEPK+LGV  GS+EK+LLK+GPYG YVQLG DRK
Sbjct: 1031 PRCKYIAKTLYGTEEEEDASQPNTRIEEPKLLGVTSGSNEKVLLKSGPYGVYVQLGEDRK 1090

Query: 2287 GYLPKRASVSQVKDTSSITLEDALELLRYPVTLGEHPDDGHPVILKLAKVGFAIKHRRTV 2466
            GY+P+RASVS +KD  SITL+DALE+L+YP+TLG HP DG PVIL+LA+ GF ++HRRT+
Sbjct: 1091 GYIPRRASVSHIKDVDSITLKDALEVLQYPLTLGNHPKDGQPVILRLARAGFCVRHRRTI 1150

Query: 2467 SPVPKNMKPEDISLDKAVQILLSKDAKRSGRPKNKPKHQEIDAIE 2601
            + +PKN+KP D++L+KA++ L   D +RSGRPK      E++ IE
Sbjct: 1151 ASIPKNVKPSDVTLEKALEYLSGDDVRRSGRPKKGKSKVEVEVIE 1195


>ref|XP_002528003.1| prokaryotic DNA topoisomerase, putative [Ricinus communis]
            gi|223532629|gb|EEF34415.1| prokaryotic DNA
            topoisomerase, putative [Ricinus communis]
          Length = 1071

 Score = 1167 bits (3020), Expect = 0.0
 Identities = 586/834 (70%), Positives = 679/834 (81%), Gaps = 2/834 (0%)
 Frame = +1

Query: 94   DPKQTPETSVKPKPYKQRVIKQLYPPTGKSVLVVESVTKAKVIQGYLGDMFEVLPSYGHV 273
            D +Q  +   K KP++Q  +K LYPPT KSV+VVESVTKAKVIQGYLG MFEVLPSYGHV
Sbjct: 261  DKQQQHKPMHKSKPFRQGSLKPLYPPTAKSVVVVESVTKAKVIQGYLGPMFEVLPSYGHV 320

Query: 274  RDLAARSGSVRPDDDFSMVWEVPSAAWTHLKSIKVALSGAKNLILASDPDREGEAIAWHI 453
            RDLAARSGSVRPDDDFSMVWEVPS AWTHLK+IKVAL+GA+NL+LASDPDREGEAIAWHI
Sbjct: 321  RDLAARSGSVRPDDDFSMVWEVPSPAWTHLKTIKVALNGAENLVLASDPDREGEAIAWHI 380

Query: 454  IEMLVQQDALREDVNLARVVFNEITESSIKTALLAPRDINANLVNSYLARRALDYLIGFN 633
            IEML QQDAL + V +ARVVF+EITE SIK AL APR+I+ NLV++YLARRALDYLIGFN
Sbjct: 381  IEMLQQQDALHQGVTVARVVFHEITEQSIKNALQAPREIDLNLVHAYLARRALDYLIGFN 440

Query: 634  ISPVLWRKLPGCRSAGRVQSAALSLICDREMEIEGFKPQEYWTVDVQF--KEKGSSLPVD 807
            ISP+LWRKLPGC+SAGRVQSAALSLICDREMEI+ F PQEYWT+DV+    E GSS    
Sbjct: 441  ISPLLWRKLPGCQSAGRVQSAALSLICDREMEIDEFTPQEYWTIDVELYIMETGSS---- 496

Query: 808  IYSIKSYLTHFQSEKLSKLSISSHANAVEIQGVIEASEFQVSNIKRSKMRKNPPTPYITS 987
               + + LTHF   KL++LS+ SH  A +I+  I  + F V+  K SKMR+NPPTPYITS
Sbjct: 497  ---VNARLTHFDFNKLNQLSVRSHTEARDIEQKINVASFLVAGAKESKMRRNPPTPYITS 553

Query: 988  SLQQDAANKLNFPATYTMRLAQKLYEGVQLPDGASAGLITYIRTDGLHISDEACKDICSL 1167
            +LQQDAANKL+F + YTM+LAQKLYEGVQL DG + GLITYIRTDGLHIS EA K+I SL
Sbjct: 554  TLQQDAANKLHFSSMYTMKLAQKLYEGVQLSDGKTTGLITYIRTDGLHISGEAVKEIHSL 613

Query: 1168 VRERYGESHASDGARKYFKKVKNAQEAHEAIRPTNIRRVPSSLSGVLDEDSLKLYTLIWS 1347
            V ERYG+  ASD  RKYFKKVKNAQEAHEA+RPTN+R +PS L+ VLDEDSLKLYTLIWS
Sbjct: 614  VIERYGQDFASDSPRKYFKKVKNAQEAHEAVRPTNVRMLPSMLANVLDEDSLKLYTLIWS 673

Query: 1348 RTVACQMEPAIFYQIQVDIGNADQSIIFRSSCSRIEFPGYQAVFEDVEARLIRNRDNEGN 1527
            RTVACQMEPA   QIQVDIGNA+ SI  RS+CS + F GYQ V+ED EA  I+N+++E N
Sbjct: 674  RTVACQMEPATIEQIQVDIGNANGSITLRSACSGVGFLGYQIVYEDKEAGAIKNKESEVN 733

Query: 1528 ERSDVSDVLSRMKSGDPLYLGKVDLKQHHTQPPPRFSEGSLVKTMEELGIGRPSTYASTM 1707
            + ++   +L  +K GDP  L +V LKQH TQPPPR+SEG+LVK +EELGIGRPSTYAST+
Sbjct: 734  DHNEAFGILKSLKPGDPFNLSEVKLKQHFTQPPPRYSEGTLVKKLEELGIGRPSTYASTL 793

Query: 1708 KVLQDRKYITVKSRVLYPEFRGRMLSAFLSNHFSEVTDYSFTADMETELDNVSAGLTEWK 1887
            KVLQDR Y+TVK+RVL PEFRGRM+SAFLS HF+EVTDYSFTADMETELDNVSAGLTEWK
Sbjct: 794  KVLQDRNYVTVKNRVLNPEFRGRMVSAFLSQHFTEVTDYSFTADMETELDNVSAGLTEWK 853

Query: 1888 GLLKDYWTRFSKYCESTSNLHIHEVEKMLEKTFGDFLFTSLPGKSRTCPSCREGTLIFKV 2067
            GLL+DYWTRFS YC    ++HIH+VEKMLEKTFGDFLF SLP  SRTCPSC EGTLIFK 
Sbjct: 854  GLLRDYWTRFSSYCSHAESVHIHQVEKMLEKTFGDFLFGSLPDDSRTCPSCLEGTLIFK- 912

Query: 2068 SRLGAGYFIGCDQHPRCKYIAKTIHGXXXXXISPDNQNVEEPKILGVKPGSDEKILLKNG 2247
                  Y +              I+G      +P N +VEEPK+LGV P S EK+LLKNG
Sbjct: 913  ------YML-------------DIYGDDEDEETPQNNSVEEPKLLGVHPVSTEKVLLKNG 953

Query: 2248 PYGFYVQLGVDRKGYLPKRASVSQVKDTSSITLEDALELLRYPVTLGEHPDDGHPVILKL 2427
            PYGFY+QLG DRKGY PKRASVS +KD  +ITLEDALELLRYPVTLG HP DGHP+ILKL
Sbjct: 954  PYGFYLQLGEDRKGYTPKRASVSHIKDVDNITLEDALELLRYPVTLGNHPKDGHPIILKL 1013

Query: 2428 AKVGFAIKHRRTVSPVPKNMKPEDISLDKAVQILLSKDAKRSGRPKNKPKHQEI 2589
            AKVGFA++HRRT++ VPKNMKP+++SL+KA+++L   D +RSGRPK KPK +E+
Sbjct: 1014 AKVGFAVRHRRTIASVPKNMKPDNVSLEKALELLSGDDVRRSGRPKRKPKVEEV 1067


>ref|XP_004164103.1| PREDICTED: DNA topoisomerase 1-like, partial [Cucumis sativus]
          Length = 1103

 Score = 1141 bits (2951), Expect = 0.0
 Identities = 559/819 (68%), Positives = 685/819 (83%), Gaps = 8/819 (0%)
 Frame = +1

Query: 154  KQLYPPTGKSVLVVESVTKAKVIQGYLGDMFEVLPSYGHVRDLAARSGSVRPDDDFSMVW 333
            KQLYPP GKSV+VVESVTKAKVIQ YLGDMF VLPS+GHVRDLAA+SGSVRPDDDFSMVW
Sbjct: 288  KQLYPPMGKSVMVVESVTKAKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVW 347

Query: 334  EVPSAAWTHLKSIKVALSGAKNLILASDPDREGEAIAWHIIEMLVQQDALREDVNLARVV 513
            EVPSAA THLKS++V+L+GA+NLILASDPDREGEAIAWHIIEML  QDAL E +++ARVV
Sbjct: 348  EVPSAALTHLKSMQVSLNGAENLILASDPDREGEAIAWHIIEMLHHQDALHEGISIARVV 407

Query: 514  FNEITESSIKTALLAPRDINANLVNSYLARRALDYLIGFNISPVLWRKLPGCRSAGRVQS 693
            F+EITE+SIK+AL +PR I+ANLV +YLARRALDYLIGFNISP+LWRKLPGCRSAGRVQS
Sbjct: 408  FHEITEASIKSALQSPRVIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQS 467

Query: 694  AALSLICDREMEIEGFKPQEYWTVDVQFKEKGSSLPVDIYSIKSYLTHFQSEKLSKLSIS 873
            AAL+LICDREMEI+ F  QEYW++D++  +K S   V+ +++ ++LTHF S KL++ +IS
Sbjct: 468  AALALICDREMEIDEFHAQEYWSIDIELNQKESCSLVEDFAVPAHLTHFDSRKLNQFAIS 527

Query: 874  SHANAVEIQGVIEASEFQVSNIKRSKMRKNPPTPYITSSLQQDAANKLNFPATYTMRLAQ 1053
            S+  A  I+  +++  FQV + K++K+R+NPP PYITS+LQQDAANK NF A+YTM+LAQ
Sbjct: 528  SNTEAKNIETALKSVNFQVLSSKKTKVRRNPPMPYITSTLQQDAANKFNFSASYTMQLAQ 587

Query: 1054 KLYEGVQLPDGASAGLITYIRTDGLHISDEACKDICSLVRERYGESHASDGARKYFKKVK 1233
            KLYEG+QL DG +AGLITY+RTDGLHISDEA KDI +L+ +RYG++   +   KYFKKVK
Sbjct: 588  KLYEGIQLADGKAAGLITYLRTDGLHISDEAVKDIRTLIMQRYGQNFVPESGPKYFKKVK 647

Query: 1234 NAQEAHEAIRPTNIRRVPSSLSGVLDEDSLKLYTLIWSRTVACQMEPAIFYQIQVDIGNA 1413
            NAQEAHEAIRPT+++R+PS L G LDEDS KLY+LIW RTVACQME +I  QIQ++ G +
Sbjct: 648  NAQEAHEAIRPTDVQRLPSMLVGTLDEDSHKLYSLIWLRTVACQMESSITEQIQIECGPS 707

Query: 1414 DQSIIFRSSCSRIEFPGYQAVFEDVEARLIRNRDNEGNERSDVSDVLSRMKSGDPLYLGK 1593
            D+SII RS+CSR++F G+Q VFED EA  ++++D+E N R ++  +L+ +K GD L L  
Sbjct: 708  DESIISRSTCSRVQFRGFQEVFEDPEADAVKHKDHEDNGRDELFRILNALKPGDQLSLAA 767

Query: 1594 VDLKQHHTQPPPRFSEGSLVKTMEELGIGRPSTYASTMKVLQDRKYITVKSRVLYPEFRG 1773
            V+LKQH TQPPPR+SEG+LVK MEELGIGRPSTYA+T+KVLQDRKY++VKSRVL+PEFRG
Sbjct: 768  VELKQHFTQPPPRYSEGALVKKMEELGIGRPSTYATTIKVLQDRKYVSVKSRVLHPEFRG 827

Query: 1774 RMLSAFLSNHFSEVTDYSFTADMETELDNVSAGLTEWKGLLKDYWTRFSKYCESTSNLHI 1953
            RM+SAFL +HFSEVTDYSFTADME ELDNVSAGLTEWKGLL+DYWTRFS Y ES +++HI
Sbjct: 828  RMVSAFLCHHFSEVTDYSFTADMENELDNVSAGLTEWKGLLRDYWTRFSSYFESVNSVHI 887

Query: 1954 HEVEKMLEKTFGDFLFTSLPGKSRTCPSCREGTLIFKVSRLGAGYFIGCDQHPRCKYIAK 2133
            H+VEKMLEK +G F+F+ LP  SRTCPSC+EGTL FK+SR GAGYF+GCDQHP+CKYIAK
Sbjct: 888  HQVEKMLEKKYGSFIFSFLPDNSRTCPSCQEGTLTFKLSRFGAGYFVGCDQHPKCKYIAK 947

Query: 2134 TIHGXXXXXISPDNQN-VEEPKILGVKPGSDEKILLKNGPYGFYVQLGVDRKGYLPKRAS 2310
            T HG        DN N ++EPK+LG+ P  +EKILLK GP+G YVQLG DRKGYLPKRAS
Sbjct: 948  TSHGE-----DEDNDNSLKEPKLLGLHPDLNEKILLKKGPFGLYVQLGEDRKGYLPKRAS 1002

Query: 2311 VSQVKDTSSITLEDALELLRYPVTLGEHPDDGHPVILKLAKVGFAIKHRRTVSPVPKNMK 2490
            +SQ+KD +SITLEDA+E L YPVTLG HP DG PVI+K+A+VGFA+ H RT + VP+N+K
Sbjct: 1003 LSQIKDVTSITLEDAVERLCYPVTLGNHPKDGQPVIIKMARVGFAVGHGRTAASVPENLK 1062

Query: 2491 PEDISLDKAVQILLSKDAKRSGRP-------KNKPKHQE 2586
            P D+ L KA+++L S + +RSG P       K K K +E
Sbjct: 1063 PNDVDLAKALELLSSSNVRRSGLPLFPNSLSKEKQKGEE 1101