BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00011001
         (1457 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002281882.1| PREDICTED: thermospermine synthase ACAULIS5 ...   571   e-160
gb|AGE09592.1| SPDSY-like protein [Eucalyptus cladocalyx]             565   e-158
ref|XP_002516774.1| spermine synthase, putative [Ricinus communi...   562   e-158
ref|XP_002312526.1| predicted protein [Populus trichocarpa] gi|2...   561   e-157
ref|XP_003536537.1| PREDICTED: thermospermine synthase ACAULIS5-...   561   e-157

>ref|XP_002281882.1| PREDICTED: thermospermine synthase ACAULIS5 [Vitis vinifera]
            gi|297737031|emb|CBI26232.3| unnamed protein product
            [Vitis vinifera]
          Length = 329

 Score =  571 bits (1472), Expect = e-160
 Identities = 275/325 (84%), Positives = 301/325 (92%)
 Frame = -1

Query: 1232 MGDISDSNISNGNGHANGMVFQRKSCWYEEEIEENLRWCFALNSILHTGSSQYQDIQLLD 1053
            MGDIS SN +  NG ++ +   RKSCWYEEEIEENLRWCFALNSILHTG++QYQDI LLD
Sbjct: 1    MGDISCSNGNEVNGKSHSLAGYRKSCWYEEEIEENLRWCFALNSILHTGATQYQDIALLD 60

Query: 1052 TKPFGKALVIDGKLQSAEIDEFIYHESLVHPALLHHQNPTSVFIMGGGEGSTARELLRHK 873
            TKPFGKALVIDGKLQSAEIDEFIYHESLVHPALLHH NP S+FIMGGGEGSTARE+LRHK
Sbjct: 61   TKPFGKALVIDGKLQSAEIDEFIYHESLVHPALLHHPNPKSIFIMGGGEGSTAREILRHK 120

Query: 872  TIDKVVMCDIDEEVVEFCKSYLVVNRVAFCDPRLELVINDARAELERREESYDVIIGDLA 693
            T++KVVMCDIDEEVV+FCKSYLVVNR AFCDPRLELVINDARAELE REE +DVIIGDLA
Sbjct: 121  TVEKVVMCDIDEEVVDFCKSYLVVNREAFCDPRLELVINDARAELESREECFDVIIGDLA 180

Query: 692  DPIEGGPCYKLYTKTFYELTVKPRLTKGGIFVTQAGPAGVFSHAEVFSCIYNTLRQVFKY 513
            DPIEGGPCY+LYTK+FYE TVKPRL+ GGIFVTQAGPAGVFSH EVFSCI+NTLRQVFK+
Sbjct: 181  DPIEGGPCYQLYTKSFYESTVKPRLSPGGIFVTQAGPAGVFSHTEVFSCIHNTLRQVFKH 240

Query: 512  VVPYSAHIPSYADIWGWVMASDTPFELSADELDLRMEQRIEGENRYLDGKTFTSASTLSK 333
            VV YSAHIPS+ADIWGWVMASD+PF L+A+ELDLRM+QRI+GENRY+DGKTF+SASTLSK
Sbjct: 241  VVAYSAHIPSFADIWGWVMASDSPFLLNAEELDLRMKQRIKGENRYIDGKTFSSASTLSK 300

Query: 332  AVRNSLNNEKHVYTEGTARFIYGHG 258
             VR SL+NE  VYTEGTARFIYGHG
Sbjct: 301  VVRTSLDNETQVYTEGTARFIYGHG 325


>gb|AGE09592.1| SPDSY-like protein [Eucalyptus cladocalyx]
          Length = 334

 Score =  565 bits (1455), Expect = e-158
 Identities = 275/329 (83%), Positives = 298/329 (90%), Gaps = 4/329 (1%)
 Frame = -1

Query: 1232 MGDISDSN-ISNGNG---HANGMVFQRKSCWYEEEIEENLRWCFALNSILHTGSSQYQDI 1065
            MGDIS SN ISNGNG    ++ +   RKSCWYEEEIEENLRWCFALNSILHTG+SQ+QDI
Sbjct: 1    MGDISVSNGISNGNGVNGKSHSLNGYRKSCWYEEEIEENLRWCFALNSILHTGASQFQDI 60

Query: 1064 QLLDTKPFGKALVIDGKLQSAEIDEFIYHESLVHPALLHHQNPTSVFIMGGGEGSTAREL 885
             LLDTKPFGKALVIDGKLQSAEIDEFIYHESLVHPALL+H NP ++FIMGGGEGSTARE+
Sbjct: 61   ALLDTKPFGKALVIDGKLQSAEIDEFIYHESLVHPALLYHPNPKTLFIMGGGEGSTAREI 120

Query: 884  LRHKTIDKVVMCDIDEEVVEFCKSYLVVNRVAFCDPRLELVINDARAELERREESYDVII 705
            LRHK ++KV MCDIDEEVV+FC+SYLVVNR AFCDPRLEL+INDARAELE R+E +DVII
Sbjct: 121  LRHKAVEKVSMCDIDEEVVDFCRSYLVVNREAFCDPRLELIINDARAELESRKECFDVII 180

Query: 704  GDLADPIEGGPCYKLYTKTFYELTVKPRLTKGGIFVTQAGPAGVFSHAEVFSCIYNTLRQ 525
            GDLADPIEGGPCYKLYTK+FYE TVKPRLT  GIFVTQAGPAG+FSH EVFSCIYNTL+Q
Sbjct: 181  GDLADPIEGGPCYKLYTKSFYEYTVKPRLTHNGIFVTQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 524  VFKYVVPYSAHIPSYADIWGWVMASDTPFELSADELDLRMEQRIEGENRYLDGKTFTSAS 345
            VFKYVVPYSAHIPSYAD WGWVMASD PF LSA+E DLRM+QRI+GENRYLDG+TF SAS
Sbjct: 241  VFKYVVPYSAHIPSYADTWGWVMASDAPFCLSAEEFDLRMKQRIKGENRYLDGRTFASAS 300

Query: 344  TLSKAVRNSLNNEKHVYTEGTARFIYGHG 258
            TLSKAVR SL NE HVYTEG ARFIYGHG
Sbjct: 301  TLSKAVRTSLENETHVYTEGAARFIYGHG 329


>ref|XP_002516774.1| spermine synthase, putative [Ricinus communis]
            gi|223543862|gb|EEF45388.1| spermine synthase, putative
            [Ricinus communis]
          Length = 336

 Score =  562 bits (1448), Expect = e-158
 Identities = 271/331 (81%), Positives = 297/331 (89%), Gaps = 6/331 (1%)
 Frame = -1

Query: 1232 MGDISDSN-ISNGNGHANGMVFQ-----RKSCWYEEEIEENLRWCFALNSILHTGSSQYQ 1071
            MGDI+ SN ISNG    NG         RKSCWYEEEIEENLRWCFALNSILHTG SQYQ
Sbjct: 1    MGDIACSNGISNGTNGVNGKGHPSPNGYRKSCWYEEEIEENLRWCFALNSILHTGVSQYQ 60

Query: 1070 DIQLLDTKPFGKALVIDGKLQSAEIDEFIYHESLVHPALLHHQNPTSVFIMGGGEGSTAR 891
            DI LLDTKPFGKALVIDGKLQSAE+DEFIYHE LVHPA+LHH N  ++FIMGGGEGSTAR
Sbjct: 61   DIALLDTKPFGKALVIDGKLQSAEVDEFIYHECLVHPAILHHSNSKTIFIMGGGEGSTAR 120

Query: 890  ELLRHKTIDKVVMCDIDEEVVEFCKSYLVVNRVAFCDPRLELVINDARAELERREESYDV 711
            E+LRH+T++KVVMCDIDEEVV+FCK+YL VNR AFCDPRLE++INDAR ELE R+E YDV
Sbjct: 121  EILRHRTVEKVVMCDIDEEVVDFCKAYLAVNREAFCDPRLEIIINDARNELESRKECYDV 180

Query: 710  IIGDLADPIEGGPCYKLYTKTFYELTVKPRLTKGGIFVTQAGPAGVFSHAEVFSCIYNTL 531
            IIGDLADPIEGGPCYKLYTK+FYELTVKPRL +GGIFVTQAGPAG+FSH EVFSCIYNTL
Sbjct: 181  IIGDLADPIEGGPCYKLYTKSFYELTVKPRLNQGGIFVTQAGPAGIFSHTEVFSCIYNTL 240

Query: 530  RQVFKYVVPYSAHIPSYADIWGWVMASDTPFELSADELDLRMEQRIEGENRYLDGKTFTS 351
            RQVFKYVVPYSAHIPSYAD WGWVMASD+P  L+AD+LDLR++Q+I+GENRYLDGKTF+S
Sbjct: 241  RQVFKYVVPYSAHIPSYADTWGWVMASDSPLVLNADDLDLRIKQKIKGENRYLDGKTFSS 300

Query: 350  ASTLSKAVRNSLNNEKHVYTEGTARFIYGHG 258
            ASTL KAVR SL+NE HVYTEGTARFIYGHG
Sbjct: 301  ASTLCKAVRKSLDNETHVYTEGTARFIYGHG 331


>ref|XP_002312526.1| predicted protein [Populus trichocarpa] gi|222852346|gb|EEE89893.1|
            predicted protein [Populus trichocarpa]
          Length = 338

 Score =  561 bits (1446), Expect = e-157
 Identities = 275/331 (83%), Positives = 299/331 (90%), Gaps = 6/331 (1%)
 Frame = -1

Query: 1232 MGDISDSN-ISNGNGHANGMVFQ-----RKSCWYEEEIEENLRWCFALNSILHTGSSQYQ 1071
            MG+IS SN I NGNG  NG         RKSCWYEEEIEENLRWCFALNSILHTG+S+YQ
Sbjct: 1    MGEISCSNGIGNGNG-VNGKTHSSLNGYRKSCWYEEEIEENLRWCFALNSILHTGASRYQ 59

Query: 1070 DIQLLDTKPFGKALVIDGKLQSAEIDEFIYHESLVHPALLHHQNPTSVFIMGGGEGSTAR 891
            DI LLDTKPFGKAL+IDGKLQSAE+DEFIYHESLVHPALLHH +P +VFIMGGGEGSTAR
Sbjct: 60   DIALLDTKPFGKALIIDGKLQSAEVDEFIYHESLVHPALLHHSSPKTVFIMGGGEGSTAR 119

Query: 890  ELLRHKTIDKVVMCDIDEEVVEFCKSYLVVNRVAFCDPRLELVINDARAELERREESYDV 711
            E+LRHKT  KVVMCDIDEEVV+FCK+YLVVNR AFCDPRLE++INDARAELE  +E YDV
Sbjct: 120  EILRHKTAVKVVMCDIDEEVVDFCKAYLVVNREAFCDPRLEVIINDARAELEIGKECYDV 179

Query: 710  IIGDLADPIEGGPCYKLYTKTFYELTVKPRLTKGGIFVTQAGPAGVFSHAEVFSCIYNTL 531
            IIGDLADPIEGGPCYKLYTK+FYE TVKP+L +GGIFVTQAGPAG+FSH EVFSCI NTL
Sbjct: 180  IIGDLADPIEGGPCYKLYTKSFYEHTVKPKLNRGGIFVTQAGPAGIFSHTEVFSCICNTL 239

Query: 530  RQVFKYVVPYSAHIPSYADIWGWVMASDTPFELSADELDLRMEQRIEGENRYLDGKTFTS 351
            +QVFKYVVPYSAHIPS+AD WGWVMASDTPF LSADELD R++QRI+GENRYLDGKTF+S
Sbjct: 240  KQVFKYVVPYSAHIPSFADTWGWVMASDTPFTLSADELDSRIKQRIKGENRYLDGKTFSS 299

Query: 350  ASTLSKAVRNSLNNEKHVYTEGTARFIYGHG 258
            ASTLSKAVR SL+NE HVYTEGTARFIYGHG
Sbjct: 300  ASTLSKAVRKSLDNETHVYTEGTARFIYGHG 330


>ref|XP_003536537.1| PREDICTED: thermospermine synthase ACAULIS5-like [Glycine max]
          Length = 334

 Score =  561 bits (1445), Expect = e-157
 Identities = 272/322 (84%), Positives = 295/322 (91%)
 Frame = -1

Query: 1223 ISDSNISNGNGHANGMVFQRKSCWYEEEIEENLRWCFALNSILHTGSSQYQDIQLLDTKP 1044
            IS+ NI+  NG ++ +   RKSCWYEEEIEE+LRWCFALNSILHTG+SQYQDI LLDTKP
Sbjct: 10   ISNGNINGVNGQSHSLNGYRKSCWYEEEIEEDLRWCFALNSILHTGASQYQDIALLDTKP 69

Query: 1043 FGKALVIDGKLQSAEIDEFIYHESLVHPALLHHQNPTSVFIMGGGEGSTARELLRHKTID 864
            FGKALVIDGKLQSAE DEFIYHE LVHPALLH+ +P +VFIMGGGEGSTARELLRHKTI+
Sbjct: 70   FGKALVIDGKLQSAETDEFIYHECLVHPALLHNPSPKNVFIMGGGEGSTARELLRHKTIN 129

Query: 863  KVVMCDIDEEVVEFCKSYLVVNRVAFCDPRLELVINDARAELERREESYDVIIGDLADPI 684
            KVVMCDIDEEVV FCKSYLVVN+ AF D RLE+VINDARAELE R+ESYDVIIGDLADPI
Sbjct: 130  KVVMCDIDEEVVNFCKSYLVVNKEAFRDQRLEVVINDARAELETRDESYDVIIGDLADPI 189

Query: 683  EGGPCYKLYTKTFYELTVKPRLTKGGIFVTQAGPAGVFSHAEVFSCIYNTLRQVFKYVVP 504
            +GGPCYKLYTK+FYEL VKPRL +GGIFVTQAGPAG+FSH EVFSCIYNTLRQVFKYVVP
Sbjct: 190  DGGPCYKLYTKSFYELIVKPRLKQGGIFVTQAGPAGIFSHTEVFSCIYNTLRQVFKYVVP 249

Query: 503  YSAHIPSYADIWGWVMASDTPFELSADELDLRMEQRIEGENRYLDGKTFTSASTLSKAVR 324
            YSAHIPSYADIWGWVMASD+P +LSADELDLRM  RI+GENRYLDGKTFTSASTLSK VR
Sbjct: 250  YSAHIPSYADIWGWVMASDSPLDLSADELDLRMRHRIKGENRYLDGKTFTSASTLSKTVR 309

Query: 323  NSLNNEKHVYTEGTARFIYGHG 258
            NSL+NE HVYTE +ARFIYGHG
Sbjct: 310  NSLDNETHVYTEESARFIYGHG 331