BLASTX nr result
ID: Bupleurum21_contig00010757
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00010757
(1530 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002265371.1| PREDICTED: acyl-CoA synthetase family member... 390 e-108
ref|XP_003627657.1| Acetyl-coenzyme A synthetase [Medicago trunc... 363 e-100
ref|XP_002325887.1| predicted protein [Populus trichocarpa] gi|2... 367 5e-99
ref|XP_003521941.1| PREDICTED: acyl-CoA synthetase family member... 342 2e-93
ref|NP_198442.2| AMP-dependent synthetase and ligase family prot... 338 3e-90
>ref|XP_002265371.1| PREDICTED: acyl-CoA synthetase family member 4 [Vitis vinifera]
gi|296088768|emb|CBI38218.3| unnamed protein product
[Vitis vinifera]
Length = 1175
Score = 390 bits (1002), Expect(2) = e-108
Identities = 224/496 (45%), Positives = 309/496 (62%), Gaps = 7/496 (1%)
Frame = -3
Query: 1528 ILECDVFLVGE-DISSEGEVFVTGVCVAVGYLNDSSTNSLGYVKLPEESSFGNLNVG-LR 1355
I C+V LVGE D S+EGE+ V G+CV +GY D + L Y L S N ++
Sbjct: 432 ISNCNVMLVGESDTSNEGEICVNGLCVNIGYFPDPNVMPLDYSNL-SHGSLCNCSINDNE 490
Query: 1354 SKYYFKSGDFSRRLKSGDLVFLGRKDRTVKVNGQRMSLEEIENIMREHHNVIDAAAILRX 1175
S+ YF++GDF+RRL+SGDLVFLGRKDRTVK+NGQR++LEEIEN +R H +V+DAA I R
Sbjct: 491 SQLYFRTGDFARRLQSGDLVFLGRKDRTVKINGQRIALEEIENALRGHPDVVDAAVIFRK 550
Query: 1174 XXXXXXXXXGYVVTKVKDEHIQFLSS-IRSWMVNKLPSSMIPGKLFCVKSLPTSSTGKVD 998
+++ K +E + L S I WMV KLP M+P F KS P S+TGKVD
Sbjct: 551 GQGELELLEAFIILKRTNESDEVLRSCIGCWMVEKLPLVMVPNNFFFTKSFPMSATGKVD 610
Query: 997 YTLLANSSSCATPIFHEAEDVQSSDFLLHIKKAFCDALMVESVSDYDDFFSLGGDSISAA 818
Y LA S S A I E ++S+D L IKKAFCDAL VE V + DDFF +GGDSI+AA
Sbjct: 611 YASLAGSISMAH-IQDEIGGIKSNDLLEVIKKAFCDALSVEKVCNDDDFFMMGGDSIAAA 669
Query: 817 HAAHNIGIDMRLLYTCRSAMKLNMAIIKQKVLCEHNKIMIALPEEDMPEPERKILLPIDT 638
+ ++N+GI+MRL+Y S KL +A++K++ I + +D+ + +
Sbjct: 670 YVSYNLGINMRLIYNFPSPSKLQVALLKKE-----GSSSIDVGIDDIGS--------LKS 716
Query: 637 DRLDVDRTESLELLLTHCEKNTEYLPL--KDLQMDSFIHQNTHSPRTLD--LWTSSSVHM 470
D D+ ++ +N + P+ K L++DS + + S D W S+SV M
Sbjct: 717 DTCDLYSSKPCGTSSKPVFENNDKYPVTSKCLKVDSNTYATSKSVIPCDGCPWNSNSVPM 776
Query: 469 TCSYCRGNRLMHSKEYEGNSFCKPIRLKKNPSEEVTLMQELWKVHLESCVDASPLLVVRE 290
CS+ R N++M E + + C + P + M+ELWKVH+ESCVDAS ++V ++
Sbjct: 777 LCSFSRCNKVMCEVESKMKNICHTTWSIEFPRNKSGFMRELWKVHMESCVDASAIVVFKD 836
Query: 289 GHIFLYIGSHSCKFLCINARSGSVEWMVKLDGRVECSAAIVDDFCQVVVGCYKGNVYFLD 110
I+L IGSHS KF+C+NA+SG + W ++L+GR+ECSAAI+DDF QVVVGCY G +YFLD
Sbjct: 837 WDIYLLIGSHSHKFVCVNAKSGFIRWGIELEGRIECSAAILDDFSQVVVGCYSGIIYFLD 896
Query: 109 ILDGNIFWTFQTGGEI 62
+GNI WTFQT GE+
Sbjct: 897 FSNGNICWTFQTRGEV 912
Score = 28.5 bits (62), Expect(2) = e-108
Identities = 13/18 (72%), Positives = 15/18 (83%)
Frame = -1
Query: 66 RLQVKSQPVEDKCRHLVW 13
R +VKSQPV DK R+LVW
Sbjct: 909 RGEVKSQPVIDKRRNLVW 926
>ref|XP_003627657.1| Acetyl-coenzyme A synthetase [Medicago truncatula]
gi|355521679|gb|AET02133.1| Acetyl-coenzyme A synthetase
[Medicago truncatula]
Length = 1224
Score = 363 bits (931), Expect(2) = e-100
Identities = 202/495 (40%), Positives = 297/495 (60%), Gaps = 6/495 (1%)
Frame = -3
Query: 1528 ILECDVFLVGED-ISSEGEVFVTGVCVAVGYLNDSSTNSLGYVKLPEESSFGNLNVGLRS 1352
I C+V L+GE+ +EGE++V G C+ GY ++S S G+VKLP+ N +S
Sbjct: 470 ITNCNVVLIGENGAPNEGELYVGGSCIFRGYYDESDIMSEGFVKLPQNYGCENSVDVFQS 529
Query: 1351 KYYFKSGDFSRRLKSGDLVFLGRKDRTVKVNGQRMSLEEIENIMREHHNVIDAAAILRXX 1172
+ YF++GD ++L SGD +FLGRKDR VKV+GQR+SLEE+EN++REH N+ DAA + R
Sbjct: 530 ELYFRTGDLVKQLPSGDFIFLGRKDRIVKVHGQRISLEEVENLLREHPNINDAAVVCRNL 589
Query: 1171 XXXXXXXXGYVVTKVKDEHIQFL-SSIRSWMVNKLPSSMIPGKLFCVKSLPTSSTGKVDY 995
+++ K K + + L +IRSWM+NKLPS +P + +S P SS+GKV+Y
Sbjct: 590 QAELVFIEAFIILKDKQQLGELLVPAIRSWMINKLPSVWLPNRFIFTESFPISSSGKVNY 649
Query: 994 TLLANSSSCATPIFHEAEDVQSSDFLLHIKKAFCDALMVESVSDYDDFFSLGGDSISAAH 815
LL +S+ + + ++ S+ L IKK F DAL+VE + + DDFF +GG+S+SAAH
Sbjct: 650 ELLVSSALLTKSVKDKVGNISCSNLLQLIKKIFHDALLVEKLCNDDDFFIMGGNSLSAAH 709
Query: 814 AAHNIGIDMRLLYTCRSAMKLNMAIIKQKVLCE-HNKIMIALPEEDMPEPERKILLPIDT 638
AHN+GID+R LY S KL MA++ ++ C HN++ L ++ I
Sbjct: 710 VAHNLGIDLRFLYYYPSPFKLCMALLHKRGSCSLHNRLDNCL----------QLDTDIQN 759
Query: 637 DRLDVDRTES---LELLLTHCEKNTEYLPLKDLQMDSFIHQNTHSPRTLDLWTSSSVHMT 467
+ + TES LE + +K P K L+ S + W S ++ +
Sbjct: 760 NDFSSNLTESSFPLESRMIPKDKVDVLFPFKRLKRGSTDVVTSGGDEPFP-WHSLAIFSS 818
Query: 466 CSYCRGNRLMHSKEYEGNSFCKPIRLKKNPSEEVTLMQELWKVHLESCVDASPLLVVREG 287
S+ R N++++ + + P M+ WKV++ESCVDASP++V +
Sbjct: 819 SSFSRCNKVLYKGQTSVMDTHQTTWSSNVPRGSRGHMKSFWKVYMESCVDASPMVVSKGS 878
Query: 286 HIFLYIGSHSCKFLCINARSGSVEWMVKLDGRVECSAAIVDDFCQVVVGCYKGNVYFLDI 107
++L+IGSHS KFLCIN RSGS++W +KL+GR+EC+AAIV DF QVVVGCY G +YFLD
Sbjct: 879 DLYLFIGSHSHKFLCINVRSGSMQWEIKLEGRIECTAAIVSDFSQVVVGCYMGKIYFLDF 938
Query: 106 LDGNIFWTFQTGGEI 62
+G+I W FQT GE+
Sbjct: 939 WNGHICWIFQTSGEV 953
Score = 29.6 bits (65), Expect(2) = e-100
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = -1
Query: 60 QVKSQPVEDKCRHLVW 13
+VKSQP+ D CR L+W
Sbjct: 952 EVKSQPIVDTCRQLIW 967
>ref|XP_002325887.1| predicted protein [Populus trichocarpa] gi|222862762|gb|EEF00269.1|
predicted protein [Populus trichocarpa]
Length = 1058
Score = 367 bits (942), Expect = 5e-99
Identities = 220/503 (43%), Positives = 316/503 (62%), Gaps = 14/503 (2%)
Frame = -3
Query: 1528 ILECDVFLVGE-DISSEGEVFVTGVCVAVGYLNDSSTNSLGYVKLPEESSFGNLNV-GLR 1355
I CDV L+ E D S+EGE++V G+CV+ GY ++S+ S P + N +V
Sbjct: 289 ISNCDVALICESDTSNEGEIYVGGLCVSNGYYSESTVTSFISAN-PHMDNICNSSVDNWG 347
Query: 1354 SKYYFKSGDFSRRLKSGDLVFLGRKDRTVKVNGQRMSLEEIENIMREHHNVIDAAAILRX 1175
+ Y+++GDF++RL++GDLVFLGR DRTVK+NGQR+ LEEIEN +R H +V DAA I R
Sbjct: 348 CQAYYRTGDFAQRLQNGDLVFLGRTDRTVKINGQRIVLEEIENTLRGHPDVADAAVISRE 407
Query: 1174 XXXXXXXXXGYVVTKVKDEHIQFL--SSIRSWMVNKLPSSMIPGKLFCVKSLPTSSTGKV 1001
++ K +++ F SSIR WMV+K+P +M+P + +SLP SSTGKV
Sbjct: 408 GPGELLFLDAILLFKEREKSEDFFVRSSIRKWMVDKVPLAMVPNRFVITESLPMSSTGKV 467
Query: 1000 DYTLLANSSSCATPIFHEAEDVQSSDFLLHIKKAFCDALMVESVSDYDDFFSLGGDSISA 821
DY LLA S + E + +SD L IKKAFCD LMVE VS DDFF++GG+SISA
Sbjct: 468 DYALLARSKFLNLHVQDEIGNA-TSDLLQIIKKAFCDGLMVEEVSCDDDFFAMGGNSISA 526
Query: 820 AHAAHNIGIDMRLLYTCRSAMKLNMAIIKQKVLCEHNKIMIALPEEDMPEPERKILL--- 650
AH ++N+GI+MRLLY + KL+ A++++K E + + + +P++ L+
Sbjct: 527 AHVSYNLGINMRLLYNFPTPSKLHAALLEKK---ESYCMEVRVDANSQLKPKKDSLVSDM 583
Query: 649 ---PIDTDRLDVDRTESLELLLTHCEKNTE--YLPLKDLQMDSFIHQNTHSPRTLD-LWT 488
P T + V +S++ + +N + + K + D I ++ + D
Sbjct: 584 AYSPNPTSPV-VPGLKSMKQPSKNPHQNNDDHTVASKRFKEDLDISISSACVKPSDGQPL 642
Query: 487 SSSVHMTCSYCRGNRLMHSKEYEGNSFCKPIRLKKNP-SEEVTLMQELWKVHLESCVDAS 311
SSS+ M CS+ R N +++ + + RL K P + + + M ELWKV++ESCVDAS
Sbjct: 643 SSSISMLCSFSRCNTVIYDENCRSRKSHQINRLAKVPRNGKGSSMHELWKVYMESCVDAS 702
Query: 310 PLLVVREGHIFLYIGSHSCKFLCINARSGSVEWMVKLDGRVECSAAIVDDFCQVVVGCYK 131
PL+VV++ ++L+IGSHS KF+C+NA SGS++W VKL+GR+E SAAIV DF QVVVGCY
Sbjct: 703 PLVVVKQQDVYLFIGSHSHKFVCVNALSGSIQWEVKLEGRIESSAAIVGDFSQVVVGCYS 762
Query: 130 GNVYFLDILDGNIFWTFQTGGEI 62
G +YFLD LDG+I WTFQT GE+
Sbjct: 763 GKIYFLDFLDGSICWTFQTCGEV 785
>ref|XP_003521941.1| PREDICTED: acyl-CoA synthetase family member 4-like [Glycine max]
Length = 1153
Score = 342 bits (876), Expect(2) = 2e-93
Identities = 201/506 (39%), Positives = 294/506 (58%), Gaps = 17/506 (3%)
Frame = -3
Query: 1528 ILECDVFLVGED--ISSEGEVFVTGVCVAVGYLND-SSTNSLGYVKLPEESSFGNLNVGL 1358
I CDV ++ + S+EGE++V G C+ Y N+ ++ S + KLP +
Sbjct: 410 ITNCDVMMLLNENGASNEGELYVGGSCIFRDYYNEPNNIMSDAFAKLPR-------SYAC 462
Query: 1357 RSKYYFKSGDFSRRLKSGDLVFLGRKDRTVKVNGQRMSLEEIENIMREHHNVIDAAAILR 1178
+ + YF++GD ++L SGD VFLGRKDR +K+NGQR++LEE+E ++REH + DAA + R
Sbjct: 463 QGQLYFRTGDLVKQLPSGDFVFLGRKDRIIKINGQRIALEEVEELLREHPYINDAAVVCR 522
Query: 1177 XXXXXXXXXXGYVVTKVKDEHIQFL-SSIRSWMVNKLPSSMIPGKLFCVKSLPTSSTGKV 1001
+++ K K+ + L +IRSWM+NKLPS ++P + F ++S P S +GKV
Sbjct: 523 NNEAELVLLEAFIILKKKERSGELLIPAIRSWMINKLPSIVLPNRFFFMESFPVSPSGKV 582
Query: 1000 DYTLLANSSSCATPIFHEAEDVQSSDFLLHIKKAFCDALMVESVSDYDDFFSLGGDSISA 821
+Y LL S+ + + ++ S+ L IKKAF DALMVE V + DDFF +GG+S+SA
Sbjct: 583 NYELLVGSALLTKNVKDKVSNIDCSNLLQLIKKAFHDALMVEKVCNDDDFFMMGGNSLSA 642
Query: 820 AHAAHNIGIDMRLLYTCRSAMKLNMAIIKQKVLCE-HNKIMIALPEEDMPEPERKILLPI 644
AH A+ +GIDM+ LY + KL MA++++K C HN++ I
Sbjct: 643 AHVAYGLGIDMKFLYYYPTPFKLCMALLQKKGSCSLHNRL--------------DCCRQI 688
Query: 643 DTDRLDVDRTESLELLLTHCEKNTEY---LPLKDLQMDSFIHQNTHSPRTLDL------- 494
+TDR D + + H E + + LKD DSF + +D+
Sbjct: 689 NTDRQDN------HISMNHAENSRPLESRMILKDNDHDSFPSKRLKRG-LIDVTSWGDES 741
Query: 493 --WTSSSVHMTCSYCRGNRLMHSKEYEGNSFCKPIRLKKNPSEEVTLMQELWKVHLESCV 320
W S S+ ++ S+ R N++++ + + P M WKV++ESCV
Sbjct: 742 FPWYSPSL-LSFSFSRCNKVLYKGKQAVIDTNQTTWSANVPRGSRGHMNNFWKVYMESCV 800
Query: 319 DASPLLVVREGHIFLYIGSHSCKFLCINARSGSVEWMVKLDGRVECSAAIVDDFCQVVVG 140
DASP+LV + I+L+IGSHS KFLCINARSGSV+W +KL GR+EC+AAIV DF QVVVG
Sbjct: 801 DASPILVFKGTDIYLFIGSHSHKFLCINARSGSVQWEIKLKGRIECTAAIVSDFSQVVVG 860
Query: 139 CYKGNVYFLDILDGNIFWTFQTGGEI 62
CY G ++FLD L+G I W FQT GE+
Sbjct: 861 CYMGKIHFLDFLNGRICWIFQTSGEV 886
Score = 28.9 bits (63), Expect(2) = 2e-93
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = -1
Query: 60 QVKSQPVEDKCRHLVW 13
+VK+QPV D CR L+W
Sbjct: 885 EVKAQPVVDTCRQLIW 900
>ref|NP_198442.2| AMP-dependent synthetase and ligase family protein [Arabidopsis
thaliana] gi|378548266|sp|F4K1G2.1|AEE19_ARATH RecName:
Full=Putative acyl-activating enzyme 19
gi|332006646|gb|AED94029.1| AMP-dependent synthetase and
ligase family protein [Arabidopsis thaliana]
Length = 1040
Score = 338 bits (866), Expect = 3e-90
Identities = 200/504 (39%), Positives = 291/504 (57%), Gaps = 15/504 (2%)
Frame = -3
Query: 1528 ILECDVFLVG-EDISSEGEVFVTGVCVAVGYLNDSSTNSLGYVKLPEESSFGNLNVGLRS 1352
I C V L+G ED EGE+ V+G+C++ GY++ SS S GYVKL S +L S
Sbjct: 291 ISNCKVVLLGDEDKPYEGEICVSGLCLSQGYMH-SSIESEGYVKLHNNSLCNHLTNDCGS 349
Query: 1351 KYYFKSGDFSRRLKSGDLVFLGRKDRTVKVNGQRMSLEEIENIMREHHNVIDAAAILRXX 1172
+ Y+++GD+ R+L SGDL+F+GR+DRTVK+NG+RM+LEEIE + + ++ +A +L
Sbjct: 350 QLYYRTGDYGRQLSSGDLIFIGRRDRTVKLNGKRMALEEIETTLELNPDIAEAVVLLSRD 409
Query: 1171 XXXXXXXXGYVV-TKVKDEHIQFLSSIRSWMVNKLPSSMIPGKLFCVKSLPTSSTGKVDY 995
+VV K + + SIR+WM KLP MIP V+ LP +S+GKVDY
Sbjct: 410 ETELASLKAFVVLNKESNSSDGIIFSIRNWMGGKLPPVMIPNHFVLVEKLPLTSSGKVDY 469
Query: 994 TLLANSSSCATPIFHEAEDVQSSDFLLHIKKAFCDALMVESVSDYDDFFSLGGDSISAAH 815
LA T + ++ L +IKKA CDAL+V+ VSD DDFF++GGDS++AAH
Sbjct: 470 EALARLKCPTTGAQDMMQSNGTNSLLQNIKKAVCDALLVKEVSDDDDFFAIGGDSLAAAH 529
Query: 814 AAHNIGIDMRLLYTCRSAMKLNMAIIKQKVLCEHNKIMIALPEEDMPEPERKILLPIDTD 635
+H++GIDMRL+Y RS +L + + +++ L E+ +K+ I++
Sbjct: 530 LSHSLGIDMRLIYQFRSPSRLLIYLSEKEG---------KLREDMQHNTTQKLDHKIESQ 580
Query: 634 RLD--VDRTESLELLLT--------HCEKNTEYLPLKDLQMDSFIHQNTHSPRTLD---L 494
+ V RT L +T CEKN LK I SP+ + L
Sbjct: 581 NGNGLVSRTVPLHSGVTSGPTPSKLQCEKNNSPKRLK-------IDYEKFSPKRMKENKL 633
Query: 493 WTSSSVHMTCSYCRGNRLMHSKEYEGNSFCKPIRLKKNPSEEVTLMQELWKVHLESCVDA 314
W S + C++ R N++ + + + P ++ MQE+WKVH+ESCVDA
Sbjct: 634 WDSGFSQIQCAFSRCNKVHSPESCSNEEANREYWSLEIPRNQMVSMQEIWKVHMESCVDA 693
Query: 313 SPLLVVREGHIFLYIGSHSCKFLCINARSGSVEWMVKLDGRVECSAAIVDDFCQVVVGCY 134
SPL+V+++ +L+IGSHS KF CI+A+SGS+ W L+GR+E SA +V DF QVV+GCY
Sbjct: 694 SPLVVLKDSKTYLFIGSHSRKFSCIDAKSGSMYWETILEGRIEGSAMVVGDFSQVVIGCY 753
Query: 133 KGNVYFLDILDGNIFWTFQTGGEI 62
KG +YFLD G++ W FQ GEI
Sbjct: 754 KGKLYFLDFSTGSLCWKFQACGEI 777