BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00010740
         (1467 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004134149.1| PREDICTED: NEDD8-activating enzyme E1 cataly...   699   0.0  
ref|XP_002517184.1| ubiquitin-activating enzyme E1c, putative [R...   689   0.0  
ref|XP_002280928.1| PREDICTED: NEDD8-activating enzyme E1 cataly...   681   0.0  
ref|XP_003610389.1| NEDD8-activating enzyme E1 catalytic subunit...   671   0.0  
ref|XP_002311839.1| predicted protein [Populus trichocarpa] gi|2...   670   0.0  

>ref|XP_004134149.1| PREDICTED: NEDD8-activating enzyme E1 catalytic subunit-like [Cucumis
            sativus] gi|449515377|ref|XP_004164726.1| PREDICTED:
            NEDD8-activating enzyme E1 catalytic subunit-like
            [Cucumis sativus]
          Length = 449

 Score =  699 bits (1805), Expect = 0.0
 Identities = 337/428 (78%), Positives = 369/428 (86%)
 Frame = +3

Query: 126  GNLVGANFDPGTDLRNDLQEYXXXXXXXXXXXXXXXXKDLAFSGFKNLEVIDMDRIEVSN 305
            GNL+GANF+PG+ LR+DLQ+Y                KDLA SGF+NLEVIDMDRIEV+N
Sbjct: 22   GNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTN 81

Query: 306  LNRQFLFRFEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDIEFYNDFSIIALGLDS 485
            LNRQFLFR EDVGKPKAEVAAKRVMERVSGVNI+PHFCRIEDK+IEFYNDF IIALGLDS
Sbjct: 82   LNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFHIIALGLDS 141

Query: 486  VEARSYINSVACSFLEYDDDDNPKEETMKPMVDGGTEGFKGHARVIMPGVTPCFECTIWL 665
            +EARSYINSVACSFLEYD DDNP EET+KPMVDGGTEGFKGHARVI+PGVTPCFECTIWL
Sbjct: 142  IEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWL 201

Query: 666  FPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEAHGGKTFDPDDPEHMQWIYTEAVKRAE 845
            FPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDE H GK+FDPDD EHM+W+Y+EA+KRAE
Sbjct: 202  FPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKSFDPDDSEHMKWVYSEALKRAE 261

Query: 846  LFGIPGVTYTLTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEG 1025
            LFGIPGVTY+LTQGVVK                CALETLKIVSGCSKTLSNYLTYNG EG
Sbjct: 262  LFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNYLTYNGAEG 321

Query: 1026 LHTKVTEFVKDKDCLVCGPGVLIELNTSVTLKKFIDQLEDHPKLLLSRVSVTYRGKNLYM 1205
            LHTKVTEFVKDKDCLVCGPGVLIEL++S+TL+KFID LEDHPKLLLS+ SVT+RGKNLYM
Sbjct: 322  LHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKLLLSKASVTHRGKNLYM 381

Query: 1206 QAPAVLEEMTRSNLDKPLYELMDKCAKDVIHVNGAAGKSGTKQSCLRKLRIVFKGIDGVI 1385
            QAP VLEEMTRSNL  PL++LM K  KDV+HV G A K+  K SCLRKLR+ FKG+DGV 
Sbjct: 382  QAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGVT 441

Query: 1386 DMDVANGS 1409
            DMD A+G+
Sbjct: 442  DMDTASGA 449


>ref|XP_002517184.1| ubiquitin-activating enzyme E1c, putative [Ricinus communis]
            gi|223543819|gb|EEF45347.1| ubiquitin-activating enzyme
            E1c, putative [Ricinus communis]
          Length = 449

 Score =  689 bits (1779), Expect = 0.0
 Identities = 331/428 (77%), Positives = 362/428 (84%)
 Frame = +3

Query: 126  GNLVGANFDPGTDLRNDLQEYXXXXXXXXXXXXXXXXKDLAFSGFKNLEVIDMDRIEVSN 305
            GNLV   FDPG  LR+DLQEY                KDLA SGFKNLEVIDMDRIEVSN
Sbjct: 22   GNLVAPTFDPGVQLRDDLQEYVRILVVGAGGLGCELLKDLALSGFKNLEVIDMDRIEVSN 81

Query: 306  LNRQFLFRFEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDIEFYNDFSIIALGLDS 485
            LNRQFLFR EDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDK+++FYNDFSII LGLDS
Sbjct: 82   LNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKELDFYNDFSIIVLGLDS 141

Query: 486  VEARSYINSVACSFLEYDDDDNPKEETMKPMVDGGTEGFKGHARVIMPGVTPCFECTIWL 665
            +EARSYIN+VACSFLEYD +DNP+++TMKPMVDGGTEGFKGHARVIMPG+TPCFECTIWL
Sbjct: 142  IEARSYINNVACSFLEYDSEDNPRDDTMKPMVDGGTEGFKGHARVIMPGITPCFECTIWL 201

Query: 666  FPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEAHGGKTFDPDDPEHMQWIYTEAVKRAE 845
            FPPQVKFPLCTLAETPRT AHCIEYAHLIKW+E H GK FDPDDPEHM+W+YTEAVKRAE
Sbjct: 202  FPPQVKFPLCTLAETPRTPAHCIEYAHLIKWNEVHSGKAFDPDDPEHMKWVYTEAVKRAE 261

Query: 846  LFGIPGVTYTLTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEG 1025
            LFGI GVTY+LTQGVVK                CALETLKI SGCSKTLSNYLTYNGVEG
Sbjct: 262  LFGIQGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIASGCSKTLSNYLTYNGVEG 321

Query: 1026 LHTKVTEFVKDKDCLVCGPGVLIELNTSVTLKKFIDQLEDHPKLLLSRVSVTYRGKNLYM 1205
            LH KVTEFVKDKDCL CGPGVLIEL+TSV L+KFID LE+HPKL LSR SVT+RGKNLYM
Sbjct: 322  LHIKVTEFVKDKDCLACGPGVLIELDTSVALQKFIDLLEEHPKLFLSRASVTHRGKNLYM 381

Query: 1206 QAPAVLEEMTRSNLDKPLYELMDKCAKDVIHVNGAAGKSGTKQSCLRKLRIVFKGIDGVI 1385
            QAP VLEEMTRSNL  PL+ELM K  KD++HV G  G++G K SC+RKLR+VFKG++G+ 
Sbjct: 382  QAPPVLEEMTRSNLSLPLFELMGKVPKDIVHVTGMTGQNGKKTSCVRKLRVVFKGMEGIT 441

Query: 1386 DMDVANGS 1409
            DMD+A G+
Sbjct: 442  DMDMAGGA 449


>ref|XP_002280928.1| PREDICTED: NEDD8-activating enzyme E1 catalytic subunit [Vitis
            vinifera] gi|297736997|emb|CBI26198.3| unnamed protein
            product [Vitis vinifera]
          Length = 449

 Score =  681 bits (1756), Expect = 0.0
 Identities = 328/428 (76%), Positives = 364/428 (85%)
 Frame = +3

Query: 126  GNLVGANFDPGTDLRNDLQEYXXXXXXXXXXXXXXXXKDLAFSGFKNLEVIDMDRIEVSN 305
            G+LVG  F+PG +LR+DL+++                KDLA SGFKNLEVIDMDRIEVSN
Sbjct: 22   GHLVGPTFEPGPELRDDLRKFATVLVVGAGGLGCELLKDLALSGFKNLEVIDMDRIEVSN 81

Query: 306  LNRQFLFRFEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDIEFYNDFSIIALGLDS 485
            LNRQFLFR +DVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDIEFYN F+IIALGLDS
Sbjct: 82   LNRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDIEFYNHFNIIALGLDS 141

Query: 486  VEARSYINSVACSFLEYDDDDNPKEETMKPMVDGGTEGFKGHARVIMPGVTPCFECTIWL 665
            +EARSYIN+VACSFLEYD DD P EET+KPMVDGGTEGFKGHARVI+PGVTPCFECTIWL
Sbjct: 142  IEARSYINAVACSFLEYDSDDKPLEETIKPMVDGGTEGFKGHARVIIPGVTPCFECTIWL 201

Query: 666  FPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEAHGGKTFDPDDPEHMQWIYTEAVKRAE 845
            FPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDE H GK FDPDDPEHM+W+Y+EAVKRAE
Sbjct: 202  FPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDPEHMKWVYSEAVKRAE 261

Query: 846  LFGIPGVTYTLTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEG 1025
            LFGIPGVTY+LTQGVVK                CALETLKIVSGCSKTLSNYLTYNG EG
Sbjct: 262  LFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNYLTYNGAEG 321

Query: 1026 LHTKVTEFVKDKDCLVCGPGVLIELNTSVTLKKFIDQLEDHPKLLLSRVSVTYRGKNLYM 1205
            LHTKVTEFVKD DCL+CGPGVLIEL+TSVTL+KF+DQL++HPKLLLS+ SV +R KNLY+
Sbjct: 322  LHTKVTEFVKDMDCLICGPGVLIELDTSVTLQKFMDQLQEHPKLLLSKASVKHREKNLYI 381

Query: 1206 QAPAVLEEMTRSNLDKPLYELMDKCAKDVIHVNGAAGKSGTKQSCLRKLRIVFKGIDGVI 1385
            QAP VLEE+TRSNL+ PL++LM K  KD++HV G+  K+  K SCLRKLR+VFKGIDG  
Sbjct: 382  QAPPVLEELTRSNLNLPLFDLMGKIRKDIVHVTGSTTKNDKKTSCLRKLRVVFKGIDGAT 441

Query: 1386 DMDVANGS 1409
            DMD A G+
Sbjct: 442  DMDTAGGA 449


>ref|XP_003610389.1| NEDD8-activating enzyme E1 catalytic subunit [Medicago truncatula]
            gi|355511444|gb|AES92586.1| NEDD8-activating enzyme E1
            catalytic subunit [Medicago truncatula]
          Length = 454

 Score =  671 bits (1730), Expect = 0.0
 Identities = 323/428 (75%), Positives = 358/428 (83%)
 Frame = +3

Query: 126  GNLVGANFDPGTDLRNDLQEYXXXXXXXXXXXXXXXXKDLAFSGFKNLEVIDMDRIEVSN 305
            GNLVG  F+PG +LR+DLQE+                KDLA SGF+NL+VIDMDRIEV+N
Sbjct: 27   GNLVGPRFEPGAELRDDLQEFAKVLVVGAGGLGCELLKDLALSGFRNLDVIDMDRIEVTN 86

Query: 306  LNRQFLFRFEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDIEFYNDFSIIALGLDS 485
            LNRQFLFR EDVGKPKAEVAAKRVMER+SGVNIVPHFCRIEDK+IEFYNDFSIIALGLDS
Sbjct: 87   LNRQFLFRLEDVGKPKAEVAAKRVMERISGVNIVPHFCRIEDKEIEFYNDFSIIALGLDS 146

Query: 486  VEARSYINSVACSFLEYDDDDNPKEETMKPMVDGGTEGFKGHARVIMPGVTPCFECTIWL 665
            +EARSYIN+VACSFLEYD DDNP+EET+KPMVDGGTEGFKGHARVIMPG+TPCFECTIWL
Sbjct: 147  IEARSYINTVACSFLEYDSDDNPREETIKPMVDGGTEGFKGHARVIMPGITPCFECTIWL 206

Query: 666  FPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEAHGGKTFDPDDPEHMQWIYTEAVKRAE 845
            FPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDE H G  FDPD+PE+M+W+Y EAVKRAE
Sbjct: 207  FPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHRGVPFDPDNPENMKWVYDEAVKRAE 266

Query: 846  LFGIPGVTYTLTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEG 1025
            LFGIPGVTY+ TQGVVK                CALETLKI + CSKTLSNYLTYNG EG
Sbjct: 267  LFGIPGVTYSFTQGVVKNIIPAIASTNAIISAACALETLKIATECSKTLSNYLTYNGSEG 326

Query: 1026 LHTKVTEFVKDKDCLVCGPGVLIELNTSVTLKKFIDQLEDHPKLLLSRVSVTYRGKNLYM 1205
            LHTKVTEF +DKDCLVCGPG+ IEL+ S+TL+KF+D LE+HPKL LS+ SVT+RGKNLYM
Sbjct: 327  LHTKVTEFERDKDCLVCGPGIRIELDPSITLQKFMDLLEEHPKLRLSKASVTHRGKNLYM 386

Query: 1206 QAPAVLEEMTRSNLDKPLYELMDKCAKDVIHVNGAAGKSGTKQSCLRKLRIVFKGIDGVI 1385
            QAP VLEEMTRSNL   L+ LM K  KD++HVNG   K+  K SCLRKLR+VFKGIDGV 
Sbjct: 387  QAPPVLEEMTRSNLTLSLFNLMGKLPKDIVHVNGMTSKNDQKTSCLRKLRVVFKGIDGVT 446

Query: 1386 DMDVANGS 1409
            DMD A G+
Sbjct: 447  DMDTAGGA 454


>ref|XP_002311839.1| predicted protein [Populus trichocarpa] gi|222851659|gb|EEE89206.1|
            predicted protein [Populus trichocarpa]
          Length = 449

 Score =  670 bits (1728), Expect = 0.0
 Identities = 327/428 (76%), Positives = 353/428 (82%)
 Frame = +3

Query: 126  GNLVGANFDPGTDLRNDLQEYXXXXXXXXXXXXXXXXKDLAFSGFKNLEVIDMDRIEVSN 305
            GNLV   F+PG  LR+DLQEY                KDLA SGFKNLEVIDMDRIEV+N
Sbjct: 22   GNLVAPTFEPGAQLRDDLQEYARILVIGAGGLGCELLKDLALSGFKNLEVIDMDRIEVTN 81

Query: 306  LNRQFLFRFEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDIEFYNDFSIIALGLDS 485
            LNRQFLFR EDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDK+I+FY DF II LGLDS
Sbjct: 82   LNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKEIDFYKDFIIIVLGLDS 141

Query: 486  VEARSYINSVACSFLEYDDDDNPKEETMKPMVDGGTEGFKGHARVIMPGVTPCFECTIWL 665
            VEARSYIN+VAC FLEYD DDNP EET+KPMVDGGTEGFKGHARVI+PG TPCFECTIWL
Sbjct: 142  VEARSYINAVACGFLEYDSDDNPIEETVKPMVDGGTEGFKGHARVIIPGSTPCFECTIWL 201

Query: 666  FPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEAHGGKTFDPDDPEHMQWIYTEAVKRAE 845
            FPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDE H GKTFDPDDPEHM+W+YTEAVKRAE
Sbjct: 202  FPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKTFDPDDPEHMKWVYTEAVKRAE 261

Query: 846  LFGIPGVTYTLTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEG 1025
            LFGI GVTY+LTQGVVK                C LETLK+ SGCSKTLSNYLTYNGVEG
Sbjct: 262  LFGIQGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKLASGCSKTLSNYLTYNGVEG 321

Query: 1026 LHTKVTEFVKDKDCLVCGPGVLIELNTSVTLKKFIDQLEDHPKLLLSRVSVTYRGKNLYM 1205
            LH KVTEFVKDKDCLVCGPGVL+EL+TSVTL+KFID LE+HPKLLLS+ SV +R  NLYM
Sbjct: 322  LHIKVTEFVKDKDCLVCGPGVLLELDTSVTLQKFIDMLEEHPKLLLSKASVRHRATNLYM 381

Query: 1206 QAPAVLEEMTRSNLDKPLYELMDKCAKDVIHVNGAAGKSGTKQSCLRKLRIVFKGIDGVI 1385
            QAP VLEEMTRSNL+ PL+ELM K  KD++HV G   K   K SCLRKL +VFKG D V 
Sbjct: 382  QAPPVLEEMTRSNLNLPLFELMGKFPKDIVHVTGTTSKDDKKTSCLRKLCLVFKGADAVT 441

Query: 1386 DMDVANGS 1409
            D+D+A G+
Sbjct: 442  DLDMAVGA 449