BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00010732
         (1737 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002270428.1| PREDICTED: cytochrome P450 704C1-like [Vitis...   702   0.0  
emb|CBI24485.3| unnamed protein product [Vitis vinifera]              697   0.0  
ref|XP_002318861.1| predicted protein [Populus trichocarpa] gi|2...   626   e-177
ref|XP_003561125.1| PREDICTED: cytochrome P450 704C1-like [Brach...   607   e-171
ref|NP_001141345.1| uncharacterized protein LOC100273436 [Zea ma...   597   e-168

>ref|XP_002270428.1| PREDICTED: cytochrome P450 704C1-like [Vitis vinifera]
          Length = 524

 Score =  702 bits (1812), Expect = 0.0
 Identities = 339/478 (70%), Positives = 406/478 (84%)
 Frame = +2

Query: 161  YIIELYSKNNRPPVAGPIILQLLNFHKLFDYLTYHAQKNITFRMITPSHSEIYTADPVIV 340
            YI E+ SK+ RPPVAGP+  QL++F KLFDY    AQK IT+RMI PSHSEIYTADPV V
Sbjct: 46   YIREVMSKDQRPPVAGPVFSQLIHFSKLFDYQASLAQKYITYRMILPSHSEIYTADPVNV 105

Query: 341  EYILKTNFSNYGKGSYNIGVLYDLLGEGIFAVDGDKWRHQRKLASHEFSTRVLRDFSTNV 520
            EYILKTNF NYGKG Y+  ++ DL G+GIFAVDG+KWRHQRKLAS+EFST+VLRDFST+V
Sbjct: 106  EYILKTNFINYGKGEYHCDIMKDLFGDGIFAVDGEKWRHQRKLASYEFSTKVLRDFSTSV 165

Query: 521  FRTNAAKLGLHICKASAAKEVINLQDLLMKSTLDSIFKVGFGFDLDTLSGNDESSTRFMK 700
            F+ NAAKL L +   +AAK++++LQD+LMKSTLDSIFKVGFG DL++LSG+D    +F+K
Sbjct: 166  FQDNAAKLVLKVSMVAAAKQMMDLQDMLMKSTLDSIFKVGFGVDLNSLSGSDVFGNQFIK 225

Query: 701  AFDDSNNLVFWRYADPLWRIKRYFNVGFEFALKQNIIIVDNFVYDVIQRKKEEMKVGKLD 880
            AFDDSN +V+WRY D LWRIKR+ N+G E ALK NI ++D+F++++I+ K+E+MK  +LD
Sbjct: 226  AFDDSNVIVYWRYVDLLWRIKRFLNLGLEAALKHNIKVIDSFIFELIRCKREQMKNKELD 285

Query: 881  GGKEDILSRFLVESKTDPEYMTDKYLRDITLSFIIAGKDTSAGTLTWFFYMLCKHPLIQE 1060
             GKEDILSRFL+ESK DP+ M D+YLRDI LSF+IAGKDTSA TLTWFFY+LCKHPL+QE
Sbjct: 286  RGKEDILSRFLLESKKDPKKMNDRYLRDIILSFVIAGKDTSADTLTWFFYLLCKHPLVQE 345

Query: 1061 KVAQEVRAATEAGNNLHPDEFSNKLTEAALDKMQYLHSALTETLRLYPAVPVDGKSAGED 1240
            KV  EVR AT+A  N+  DEF+ K+TE +LDKMQYLH+ALTETLRLYPAVP+DGK + ED
Sbjct: 346  KVVLEVREATKAQINIPADEFAKKITEKSLDKMQYLHAALTETLRLYPAVPMDGKHSEED 405

Query: 1241 DILPDGLEIKKGDGVNYMAYAMGRMSSIWGVDAEEFRPERWLDNGVFKSESPYKFTAFQA 1420
            DILPDG ++KKGDG+ YMAYAMGRM  IWG DA EFRPERWL+NGVF+ ESP+KFTAFQA
Sbjct: 406  DILPDGFKVKKGDGMCYMAYAMGRMRCIWGEDAGEFRPERWLENGVFQPESPFKFTAFQA 465

Query: 1421 GPRICLGKEFAYRQMKILAAVLLYLFKFRLVNEGEEATYRTMLTLHMSNGLHLYAIPR 1594
            GPRICLGKEFAYRQ+KILAAVL+Y FKF+LV+E +EA YRTM TLHM  GLHLYA PR
Sbjct: 466  GPRICLGKEFAYRQLKILAAVLVYFFKFKLVDERKEARYRTMFTLHMDQGLHLYAFPR 523


>emb|CBI24485.3| unnamed protein product [Vitis vinifera]
          Length = 493

 Score =  697 bits (1799), Expect = 0.0
 Identities = 336/472 (71%), Positives = 402/472 (85%)
 Frame = +2

Query: 179  SKNNRPPVAGPIILQLLNFHKLFDYLTYHAQKNITFRMITPSHSEIYTADPVIVEYILKT 358
            SK+ RPPVAGP+  QL++F KLFDY    AQK IT+RMI PSHSEIYTADPV VEYILKT
Sbjct: 2    SKDQRPPVAGPVFSQLIHFSKLFDYQASLAQKYITYRMILPSHSEIYTADPVNVEYILKT 61

Query: 359  NFSNYGKGSYNIGVLYDLLGEGIFAVDGDKWRHQRKLASHEFSTRVLRDFSTNVFRTNAA 538
            NF NYGKG Y+  ++ DL G+GIFAVDG+KWRHQRKLAS+EFST+VLRDFST+VF+ NAA
Sbjct: 62   NFINYGKGEYHCDIMKDLFGDGIFAVDGEKWRHQRKLASYEFSTKVLRDFSTSVFQDNAA 121

Query: 539  KLGLHICKASAAKEVINLQDLLMKSTLDSIFKVGFGFDLDTLSGNDESSTRFMKAFDDSN 718
            KL L +   +AAK++++LQD+LMKSTLDSIFKVGFG DL++LSG+D    +F+KAFDDSN
Sbjct: 122  KLVLKVSMVAAAKQMMDLQDMLMKSTLDSIFKVGFGVDLNSLSGSDVFGNQFIKAFDDSN 181

Query: 719  NLVFWRYADPLWRIKRYFNVGFEFALKQNIIIVDNFVYDVIQRKKEEMKVGKLDGGKEDI 898
             +V+WRY D LWRIKR+ N+G E ALK NI ++D+F++++I+ K+E+MK  +LD GKEDI
Sbjct: 182  VIVYWRYVDLLWRIKRFLNLGLEAALKHNIKVIDSFIFELIRCKREQMKNKELDRGKEDI 241

Query: 899  LSRFLVESKTDPEYMTDKYLRDITLSFIIAGKDTSAGTLTWFFYMLCKHPLIQEKVAQEV 1078
            LSRFL+ESK DP+ M D+YLRDI LSF+IAGKDTSA TLTWFFY+LCKHPL+QEKV  EV
Sbjct: 242  LSRFLLESKKDPKKMNDRYLRDIILSFVIAGKDTSADTLTWFFYLLCKHPLVQEKVVLEV 301

Query: 1079 RAATEAGNNLHPDEFSNKLTEAALDKMQYLHSALTETLRLYPAVPVDGKSAGEDDILPDG 1258
            R AT+A  N+  DEF+ K+TE +LDKMQYLH+ALTETLRLYPAVP+DGK + EDDILPDG
Sbjct: 302  REATKAQINIPADEFAKKITEKSLDKMQYLHAALTETLRLYPAVPMDGKHSEEDDILPDG 361

Query: 1259 LEIKKGDGVNYMAYAMGRMSSIWGVDAEEFRPERWLDNGVFKSESPYKFTAFQAGPRICL 1438
             ++KKGDG+ YMAYAMGRM  IWG DA EFRPERWL+NGVF+ ESP+KFTAFQAGPRICL
Sbjct: 362  FKVKKGDGMCYMAYAMGRMRCIWGEDAGEFRPERWLENGVFQPESPFKFTAFQAGPRICL 421

Query: 1439 GKEFAYRQMKILAAVLLYLFKFRLVNEGEEATYRTMLTLHMSNGLHLYAIPR 1594
            GKEFAYRQ+KILAAVL+Y FKF+LV+E +EA YRTM TLHM  GLHLYA PR
Sbjct: 422  GKEFAYRQLKILAAVLVYFFKFKLVDERKEARYRTMFTLHMDQGLHLYAFPR 473


>ref|XP_002318861.1| predicted protein [Populus trichocarpa] gi|222859534|gb|EEE97081.1|
            predicted protein [Populus trichocarpa]
          Length = 493

 Score =  626 bits (1615), Expect = e-177
 Identities = 312/475 (65%), Positives = 383/475 (80%), Gaps = 3/475 (0%)
 Frame = +2

Query: 161  YIIELYSKNNRPPVAGPIILQLLNFHKLFDYLTYHAQKNITFRMITPSHSEIYTADPVIV 340
            +I E   ++ +PP+AGPI+  L++F++LFDY T  A+K+ TFR+ITPSHSEIYT DP+ V
Sbjct: 22   HIKESILEDQKPPIAGPILNYLVHFNRLFDYQTSIAKKHSTFRLITPSHSEIYTVDPLNV 81

Query: 341  EYILKTNFSNYGKGS---YNIGVLYDLLGEGIFAVDGDKWRHQRKLASHEFSTRVLRDFS 511
            EYIL T FSNY K S   Y + +  DL G+GIFAVDG K RHQRKLAS+EFSTRVLRD S
Sbjct: 82   EYILITKFSNYEKVSGTCYYLRLYQDLFGDGIFAVDGHKSRHQRKLASYEFSTRVLRDLS 141

Query: 512  TNVFRTNAAKLGLHICKASAAKEVINLQDLLMKSTLDSIFKVGFGFDLDTLSGNDESSTR 691
            + VFRTNAAKL   I  A+ A + I+LQD+LMKSTLDSIFKVGFGF+L+ LSG DE  +R
Sbjct: 142  SAVFRTNAAKLVSKITVAATALKSIDLQDMLMKSTLDSIFKVGFGFELNALSGLDEFGSR 201

Query: 692  FMKAFDDSNNLVFWRYADPLWRIKRYFNVGFEFALKQNIIIVDNFVYDVIQRKKEEMKVG 871
            F KAFDDSN+++FWRY D +  +KR+ N G E +LKQNI ++++F++++I+ K+E+MK G
Sbjct: 202  FTKAFDDSNSIIFWRYVDLILELKRFLNFGSEASLKQNIKVINDFIFELIRCKREQMKTG 261

Query: 872  KLDGGKEDILSRFLVESKTDPEYMTDKYLRDITLSFIIAGKDTSAGTLTWFFYMLCKHPL 1051
            KL+  +EDILSRFL+ES+ DPE MTD+YLRDITL+FIIAGKDTSA TL WFFYMLCKHPL
Sbjct: 262  KLEAREEDILSRFLLESEKDPENMTDQYLRDITLNFIIAGKDTSANTLAWFFYMLCKHPL 321

Query: 1052 IQEKVAQEVRAATEAGNNLHPDEFSNKLTEAALDKMQYLHSALTETLRLYPAVPVDGKSA 1231
            +QEKV QEVR A     ++  DEFS  +TE ALDKMQYLH++LTETLRLYPAVP+DGKSA
Sbjct: 322  VQEKVVQEVREAVGIKESMSADEFSKLMTEEALDKMQYLHASLTETLRLYPAVPLDGKSA 381

Query: 1232 GEDDILPDGLEIKKGDGVNYMAYAMGRMSSIWGVDAEEFRPERWLDNGVFKSESPYKFTA 1411
             ED+ILP+G ++KKGDG+ YMAY MGRM +IWG DAEEF PERWL +G+F    P+KFTA
Sbjct: 382  AEDEILPNGFKVKKGDGITYMAYVMGRMKNIWGDDAEEFHPERWLHDGIF---LPFKFTA 438

Query: 1412 FQAGPRICLGKEFAYRQMKILAAVLLYLFKFRLVNEGEEATYRTMLTLHMSNGLH 1576
              AGPRICLGKEFA RQMKILAAVLLY F+F+LV+  +EATYRTM TLH+  GLH
Sbjct: 439  VHAGPRICLGKEFADRQMKILAAVLLYFFRFKLVDARKEATYRTMFTLHLDKGLH 493


>ref|XP_003561125.1| PREDICTED: cytochrome P450 704C1-like [Brachypodium distachyon]
          Length = 525

 Score =  607 bits (1564), Expect = e-171
 Identities = 293/488 (60%), Positives = 378/488 (77%), Gaps = 5/488 (1%)
 Frame = +2

Query: 149  NFASYIIELYSKN--NRPPVAGPIILQLLNFHKLFDYLTYHAQKNITFRMITPSHSEIYT 322
            +FA + I  +++   +RPP+ G +  QL NF ++FD    +A ++ T R++ P HSEI T
Sbjct: 37   SFAVFCIREFAQRAQDRPPLIGTVFRQLKNFDRIFDEHVKYALQHPTTRLVYPGHSEILT 96

Query: 323  ADPVIVEYILKTNFSNYGKGSYNIGVLYDLLGEGIFAVDGDKWRHQRKLASHEFSTRVLR 502
            ADP ++E++LKTNFSNY KG++N  +  DL G GIFA DG+KWRHQRKLASHEFST+VLR
Sbjct: 97   ADPAVIEHVLKTNFSNYSKGAFNTEIAKDLFGNGIFATDGEKWRHQRKLASHEFSTKVLR 156

Query: 503  DFSTNVFRTNAAKLGLHICKASAAKEVINLQDLLMKSTLDSIFKVGFGFDLDTLSGNDES 682
            DFS++VFR NAAKL   I  A+A +  IN+QDLLM++T+DSIFKVGFGF+L+TL G DES
Sbjct: 157  DFSSDVFRMNAAKLSEKISCAAAKRITINMQDLLMRTTMDSIFKVGFGFELNTLYGTDES 216

Query: 683  STRFMKAFDDSNNLVFWRYADPLWRIKRYFNVGFEFALKQNIIIVDNFVYDVIQRKKEEM 862
            S  F KAFD++N+LV++RY D  W++KRYFN+G E  LK++I I+DNFV  +I +KKE+M
Sbjct: 217  SIEFSKAFDEANSLVYYRYVDLFWKLKRYFNIGSEAKLKKSIQIIDNFVIHLIHQKKEKM 276

Query: 863  KVGKLDGGKEDILSRFLVESKTDPEYMTDKYLRDITLSFIIAGKDTSAGTLTWFFYMLCK 1042
            K G     +EDILSRF+ ES+ DP+ M D+YLRDI LSF+IAGKDT+  TL+WFFYMLCK
Sbjct: 277  KNGSDHKAREDILSRFIQESEKDPQTMNDRYLRDIVLSFLIAGKDTTGDTLSWFFYMLCK 336

Query: 1043 HPLIQEKVAQEVRAATE---AGNNLHPDEFSNKLTEAALDKMQYLHSALTETLRLYPAVP 1213
            +P++Q+K+A E+R + E     NN+  + F+ +L + A+DKM YLH+A+TETLRLYP VP
Sbjct: 337  NPVVQDKIAFEIRESVEWVQEDNNM--EMFTARLKQGAIDKMHYLHAAITETLRLYPGVP 394

Query: 1214 VDGKSAGEDDILPDGLEIKKGDGVNYMAYAMGRMSSIWGVDAEEFRPERWLDNGVFKSES 1393
            VDGK A EDD+LP+G  + KGDG+NYM YAMGRM  +WG DAEEFRPERWL N VF+ ES
Sbjct: 395  VDGKMADEDDVLPNGYRVMKGDGMNYMIYAMGRMKYLWGEDAEEFRPERWLVNAVFQHES 454

Query: 1394 PYKFTAFQAGPRICLGKEFAYRQMKILAAVLLYLFKFRLVNEGEEATYRTMLTLHMSNGL 1573
            PYKF AF AGPRICLGKEFAYRQMKI+AA LL+ F+FRL +E +  TY+ M TLHM  GL
Sbjct: 455  PYKFVAFNAGPRICLGKEFAYRQMKIVAASLLHFFRFRLEDESKGPTYKPMFTLHMDKGL 514

Query: 1574 HLYAIPRQ 1597
            HL+A PR+
Sbjct: 515  HLFACPRK 522


>ref|NP_001141345.1| uncharacterized protein LOC100273436 [Zea mays]
            gi|194704096|gb|ACF86132.1| unknown [Zea mays]
            gi|413942718|gb|AFW75367.1| putative cytochrome P450
            superfamily protein [Zea mays]
          Length = 522

 Score =  597 bits (1539), Expect = e-168
 Identities = 277/469 (59%), Positives = 377/469 (80%), Gaps = 1/469 (0%)
 Frame = +2

Query: 191  RPPVAGPIILQLLNFHKLFDYLTYHAQKNITFRMITPSHSEIYTADPVIVEYILKTNFSN 370
            RPP+ G ++ QL NF +LFD    +A  + T R++ P HSE++TADP +VE++L+TNFS 
Sbjct: 53   RPPLTGTMLRQLKNFDRLFDEHVRYALAHRTSRLVYPGHSELFTADPAVVEHVLRTNFSK 112

Query: 371  YGKGSYNIGVLYDLLGEGIFAVDGDKWRHQRKLASHEFSTRVLRDFSTNVFRTNAAKLGL 550
            Y KG+YNIGV+ DL G+GIFA+DGD WRHQRKLASHEFST+VLR+FS+ VFR NA +L  
Sbjct: 113  YSKGAYNIGVMKDLFGDGIFAIDGDSWRHQRKLASHEFSTKVLREFSSVVFRANATRLVD 172

Query: 551  HICKASAAKEVINLQDLLMKSTLDSIFKVGFGFDLDTLSGNDESSTRFMKAFDDSNNLVF 730
             I  A+A + ++N+QDLLMK+T+DSIFKVGFGF+L+TLSG+D+SS +F  AFD++N +V+
Sbjct: 173  KISSAAANRTILNMQDLLMKTTMDSIFKVGFGFELNTLSGSDKSSVQFSNAFDEANCIVY 232

Query: 731  WRYADPLWRIKRYFNVGFEFALKQNIIIVDNFVYDVIQRKKEEMKVGKLDGGKEDILSRF 910
             RY D  W++KRYFN+G E  L++NI I+D+FV ++I +K+E+M  G+ +  +EDILSRF
Sbjct: 233  HRYVDLFWQLKRYFNIGSEAKLRKNIQIIDDFVMNLIHQKREQMN-GQDNKAREDILSRF 291

Query: 911  LVESKTDPEYMTDKYLRDITLSFIIAGKDTSAGTLTWFFYMLCKHPLIQEKVAQEVRAAT 1090
            ++ SK DPE + D YLRDI L+F+IAGKDT+  TL+WF YMLCK+P++Q+KVA E++ + 
Sbjct: 292  IIASKEDPEMINDCYLRDIVLNFLIAGKDTTGNTLSWFLYMLCKNPIVQDKVALEIKESV 351

Query: 1091 E-AGNNLHPDEFSNKLTEAALDKMQYLHSALTETLRLYPAVPVDGKSAGEDDILPDGLEI 1267
            E A  + + ++F+ +L + A+DKM YLH+A++ETLRLYPAVPVDGK A +DD+LP+G ++
Sbjct: 352  EWAEEDNNREDFTARLNDRAIDKMHYLHAAISETLRLYPAVPVDGKMAEDDDVLPNGYKV 411

Query: 1268 KKGDGVNYMAYAMGRMSSIWGVDAEEFRPERWLDNGVFKSESPYKFTAFQAGPRICLGKE 1447
             KGDG+NYM YAMGRM+ +WG DAE+FRPERW+ NGVF+ ESPYKF +F AGPR CLGK+
Sbjct: 412  IKGDGINYMIYAMGRMTYLWGEDAEDFRPERWIANGVFQQESPYKFVSFNAGPRTCLGKD 471

Query: 1448 FAYRQMKILAAVLLYLFKFRLVNEGEEATYRTMLTLHMSNGLHLYAIPR 1594
            FAYRQMKI+AA L++ F+F+L +E ++ATY+TM TLHM  GL LYA PR
Sbjct: 472  FAYRQMKIMAATLIHFFRFKLTDESKDATYKTMFTLHMDKGLKLYAYPR 520