BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00010675
         (1563 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002268712.1| PREDICTED: GPI transamidase component PIG-S-...   699   0.0  
ref|XP_004138391.1| PREDICTED: GPI transamidase component PIG-S-...   664   0.0  
emb|CBI18338.3| unnamed protein product [Vitis vinifera]              658   0.0  
ref|XP_002303053.1| predicted protein [Populus trichocarpa] gi|2...   648   0.0  
ref|XP_004160758.1| PREDICTED: GPI transamidase component PIG-S-...   646   0.0  

>ref|XP_002268712.1| PREDICTED: GPI transamidase component PIG-S-like [Vitis vinifera]
          Length = 611

 Score =  699 bits (1803), Expect = 0.0
 Identities = 356/514 (69%), Positives = 414/514 (80%), Gaps = 4/514 (0%)
 Frame = -1

Query: 1563 VFVDFDYSEVSADDLGFSILGHMRKLTNNDHVCGNCGN-NYSVSVTMESGGDCKQSESSD 1387
            +FV FD     A +LG SI   M+KLT +  VCG C + NY+VSVT++SG  C ++ +++
Sbjct: 98   IFVGFD--NFDAYELGISIKHEMKKLTKHP-VCGTCSDSNYTVSVTVDSGSGCARNYNAE 154

Query: 1386 LSGLWQCGRIKGSNLDEVVKNDESFDEYLYSMLGGSDEGSGNSGKVYSVVVLRRDDNIRA 1207
             + +W+CG I   +L     +D+  DE+L S+LG  +       KVYSVVV+ R   +RA
Sbjct: 155  STCMWRCGAIGEFDLGGG-GDDDGVDEFLGSVLGSCE----GFRKVYSVVVVNRGGEVRA 209

Query: 1206 VIGKNRHAWIVGRVLE--MDATVEMVAEVFVKIFVNGGVRSGSIPAEFMPVGADGKIVLS 1033
            V+GK RHAWI+G VLE  M+A V  VAE FVK+FVNGG   GSI  EFMPVGADG+IVLS
Sbjct: 210  VVGKYRHAWIIGGVLEEGMNAMVARVAETFVKVFVNGGKEEGSIHGEFMPVGADGRIVLS 269

Query: 1032 FNLLNADPRDWIYDWSFQHIDDTLLAPIVKTLAPLANISIESQVLYHTPKSSFAYWDKEK 853
            FNLLNADP DWIY W FQ ID+ LLAP++K L P+ANIS+ESQVLYHTPKSSF+YWD++ 
Sbjct: 270  FNLLNADPTDWIYGWDFQRIDEILLAPVIKALGPVANISVESQVLYHTPKSSFSYWDEKW 329

Query: 852  DSYIFSTKDLPFFVNSNEWHLDTSIAAGGRSKILQFVVYVPSAEECPLLLQLPNGEISET 673
            DSYIFSTKDLPFFVNSNEWHLDTSIAAGGRSKILQFVVY+PSA+ECPLLLQLPNGEIS T
Sbjct: 330  DSYIFSTKDLPFFVNSNEWHLDTSIAAGGRSKILQFVVYIPSAKECPLLLQLPNGEISVT 389

Query: 672  NGFISPMWGGVIVWNPPGCVRDGQNLH-LRKKMSLQDLEKVFEVFVGQLRQLFGLNSGSL 496
            N FISPMWGGV VWNPPGC RD ++ H  R  +S QDL+KVFE+F+GQLRQLFGL S SL
Sbjct: 390  NAFISPMWGGVAVWNPPGCSRDSESKHPARHTISPQDLQKVFEIFMGQLRQLFGLKSDSL 449

Query: 495  YRDASKTFTLLSSGKGITNWELDFLSRQHTCYNLLQCGTTLGSLSRLVQSLPRMIIKDEI 316
            Y  AS T  LL+S +G T WELD LSRQHTC+NL+ C TTLGSLSRLVQSLPRMII DEI
Sbjct: 450  YAGASGTTNLLASERGFTEWELDVLSRQHTCFNLVSCATTLGSLSRLVQSLPRMIIMDEI 509

Query: 315  GKQVKFSLEAAKLARNNASYGIYDASAVSSREARILAEDAFFHPSMMSVSYYSFEHCFAV 136
            GKQVKFSLEAAKL ++NAS GIYDASAVSSR+AR LAEDAFFHPS+MSVSYYSFEHCFAV
Sbjct: 510  GKQVKFSLEAAKLTQSNASLGIYDASAVSSRQARSLAEDAFFHPSIMSVSYYSFEHCFAV 569

Query: 135  YSPFFLPVSMHILLAVLREFRRYKQESAKHRAWR 34
            YSPFFLPVSMH+LLA  RE+RRYKQE+AK+ AW+
Sbjct: 570  YSPFFLPVSMHVLLAAFREWRRYKQETAKYLAWK 603


>ref|XP_004138391.1| PREDICTED: GPI transamidase component PIG-S-like [Cucumis sativus]
          Length = 609

 Score =  664 bits (1713), Expect = 0.0
 Identities = 323/513 (62%), Positives = 403/513 (78%), Gaps = 2/513 (0%)
 Frame = -1

Query: 1563 VFVDFDYSEVSADDLGFSILGHMRKLTNNDHVCGNCGNNYSVSVTMESGGDCKQSESSDL 1384
            +F  FD     A+ L  SIL  M KL++   +CG+C NNY+VSV +ESG DC Q+ +   
Sbjct: 94   IFFGFDSMASRAEQLKSSILDEMTKLSSKSSLCGSCSNNYAVSVVIESGSDCSQTRTDAS 153

Query: 1383 SGLWQCGRIKGSNLDEVVKND-ESFDEYLYSMLGGSDEGSGNSGKVYSVVVLRRDDNIRA 1207
            S  W+CG +  S+    ++N  +S D++L   LGG  + + + G+VYSVVV+ + +N++A
Sbjct: 154  SCSWRCGALSASDFAASLENGLQSADDFLEVALGGCYKPA-SGGRVYSVVVMNKGENVKA 212

Query: 1206 VIGKNRHAWIVGRVLEMDATVEMVAEVFVKIFVNGGVRSGSIPAEFMPVGADGKIVLSFN 1027
             IGK RH WIVGRV E +A +  VAE FVK+F NGG   G IP EFMPVGADGKI LSFN
Sbjct: 213  TIGKYRHGWIVGRVSEAEA-IAKVAETFVKLFGNGGTEDGLIPGEFMPVGADGKIFLSFN 271

Query: 1026 LLNADPRDWIYDWSFQHIDDTLLAPIVKTLAPLANISIESQVLYHTPKSSFAYWDKEKDS 847
            LLNADP DWIYDW FQ +D+ +L P+++ L P+AN+S+ESQVLYHTP SSF+YWD +++S
Sbjct: 272  LLNADPDDWIYDWDFQKVDEVILKPLIEELTPIANVSVESQVLYHTPTSSFSYWDNKQES 331

Query: 846  YIFSTKDLPFFVNSNEWHLDTSIAAGGRSKILQFVVYVPSAEECPLLLQLPNGEISETNG 667
            YIF+TKDLPFFVNSNEWHLDTSIAAGGRSKIL FVVY+PSA ECPLLLQLP+G+ISETNG
Sbjct: 332  YIFNTKDLPFFVNSNEWHLDTSIAAGGRSKILHFVVYIPSARECPLLLQLPDGQISETNG 391

Query: 666  FISPMWGGVIVWNPPGCVRDGQNLHLRKKMSL-QDLEKVFEVFVGQLRQLFGLNSGSLYR 490
            FISP WGGVIVWNP GC+RD ++  L + M L  +LEK+ EVF+GQ RQLFGL S   + 
Sbjct: 392  FISPTWGGVIVWNPKGCLRDHESKLLHRHMILYPELEKIVEVFLGQFRQLFGLKSNPQHV 451

Query: 489  DASKTFTLLSSGKGITNWELDFLSRQHTCYNLLQCGTTLGSLSRLVQSLPRMIIKDEIGK 310
              S TF +L+S KG T WE+DFLSRQH+C+NL  C ++LGSLSRLVQSLPRMII DEIGK
Sbjct: 452  GLSGTFNILTSQKGFTEWEMDFLSRQHSCFNLHSCASSLGSLSRLVQSLPRMIIIDEIGK 511

Query: 309  QVKFSLEAAKLARNNASYGIYDASAVSSREARILAEDAFFHPSMMSVSYYSFEHCFAVYS 130
            QVK+SLEAA LA+ NAS G++DA+A+SSR+AR LAEDAFFHPS+MSVSY+SFEHCFAVYS
Sbjct: 512  QVKYSLEAANLAQKNASMGVFDAAAISSRQARSLAEDAFFHPSIMSVSYFSFEHCFAVYS 571

Query: 129  PFFLPVSMHILLAVLREFRRYKQESAKHRAWRA 31
            PFFLPV++H++LA +RE++RYKQE  K+ A+ A
Sbjct: 572  PFFLPVALHVILAAVREWKRYKQEHKKYIAFLA 604


>emb|CBI18338.3| unnamed protein product [Vitis vinifera]
          Length = 520

 Score =  658 bits (1697), Expect = 0.0
 Identities = 329/476 (69%), Positives = 378/476 (79%), Gaps = 3/476 (0%)
 Frame = -1

Query: 1452 NNYSVSVTMESGGDCKQSESSDLSGLWQCGRIKGSNLDEVVKNDESFDEYLYSMLGGSDE 1273
            +NY+VSVT++SG  C ++ +++ + +W+C   +                           
Sbjct: 70   SNYTVSVTVDSGSGCARNYNAESTCMWRCEGFR--------------------------- 102

Query: 1272 GSGNSGKVYSVVVLRRDDNIRAVIGKNRHAWIVGRVLE--MDATVEMVAEVFVKIFVNGG 1099
                  KVYSVVV+ R   +RAV+GK RHAWI+G VLE  M+A V  VAE FVK+FVNGG
Sbjct: 103  ------KVYSVVVVNRGGEVRAVVGKYRHAWIIGGVLEEGMNAMVARVAETFVKVFVNGG 156

Query: 1098 VRSGSIPAEFMPVGADGKIVLSFNLLNADPRDWIYDWSFQHIDDTLLAPIVKTLAPLANI 919
               GSI  EFMPVGADG+IVLSFNLLNADP DWIY W FQ ID+ LLAP++K L P+ANI
Sbjct: 157  KEEGSIHGEFMPVGADGRIVLSFNLLNADPTDWIYGWDFQRIDEILLAPVIKALGPVANI 216

Query: 918  SIESQVLYHTPKSSFAYWDKEKDSYIFSTKDLPFFVNSNEWHLDTSIAAGGRSKILQFVV 739
            S+ESQVLYHTPKSSF+YWD++ DSYIFSTKDLPFFVNSNEWHLDTSIAAGGRSKILQFVV
Sbjct: 217  SVESQVLYHTPKSSFSYWDEKWDSYIFSTKDLPFFVNSNEWHLDTSIAAGGRSKILQFVV 276

Query: 738  YVPSAEECPLLLQLPNGEISETNGFISPMWGGVIVWNPPGCVRDGQNLH-LRKKMSLQDL 562
            Y+PSA+ECPLLLQLPNGEIS TN FISPMWGGV VWNPPGC RD ++ H  R  +S QDL
Sbjct: 277  YIPSAKECPLLLQLPNGEISVTNAFISPMWGGVAVWNPPGCSRDSESKHPARHTISPQDL 336

Query: 561  EKVFEVFVGQLRQLFGLNSGSLYRDASKTFTLLSSGKGITNWELDFLSRQHTCYNLLQCG 382
            +KVFE+F+GQLRQLFGL S SLY  AS T  LL+S +G T WELD LSRQHTC+NL+ C 
Sbjct: 337  QKVFEIFMGQLRQLFGLKSDSLYAGASGTTNLLASERGFTEWELDVLSRQHTCFNLVSCA 396

Query: 381  TTLGSLSRLVQSLPRMIIKDEIGKQVKFSLEAAKLARNNASYGIYDASAVSSREARILAE 202
            TTLGSLSRLVQSLPRMII DEIGKQVKFSLEAAKL ++NAS GIYDASAVSSR+AR LAE
Sbjct: 397  TTLGSLSRLVQSLPRMIIMDEIGKQVKFSLEAAKLTQSNASLGIYDASAVSSRQARSLAE 456

Query: 201  DAFFHPSMMSVSYYSFEHCFAVYSPFFLPVSMHILLAVLREFRRYKQESAKHRAWR 34
            DAFFHPS+MSVSYYSFEHCFAVYSPFFLPVSMH+LLA  RE+RRYKQE+AK+ AW+
Sbjct: 457  DAFFHPSIMSVSYYSFEHCFAVYSPFFLPVSMHVLLAAFREWRRYKQETAKYLAWK 512


>ref|XP_002303053.1| predicted protein [Populus trichocarpa] gi|222844779|gb|EEE82326.1|
            predicted protein [Populus trichocarpa]
          Length = 584

 Score =  648 bits (1672), Expect = 0.0
 Identities = 333/486 (68%), Positives = 385/486 (79%), Gaps = 4/486 (0%)
 Frame = -1

Query: 1467 CGNCGNNYSVSVTMESGGDCKQSESSDLSGLWQCGRIKGSNLDEVVKNDESFDEYLYSML 1288
            CG C NN+++S+T++ GG C QS S      ++CG I+  +LD    +DES DE L S  
Sbjct: 109  CGACTNNFTLSLTLDDGG-CTQSSSK----FYKCGAIRAVDLD--FGDDESVDEALES-- 159

Query: 1287 GGSDEGSGNSGKVYSVVVLRRDDNI---RAVIGKNRHAWIVGRVLEMDATVEMVAEVFVK 1117
             G D G    GKVYSVVV+   D +   + V+GK RHAWIVGR   ++   E +AE+FV+
Sbjct: 160  AGLDSG----GKVYSVVVVVNGDGVEGVKVVVGKYRHAWIVGRDWGVEEVAERLAEIFVR 215

Query: 1116 IFVNGGVRSGSIPAEFMPVGADGKIVLSFNLLNADPRDWIYDWSFQHIDDTLLAPIVKTL 937
            +FVNGG   G I  EFMPVGADG+IVLSFNLLNADP DW YDW F+ ID+TLLAP++  L
Sbjct: 216  VFVNGGREEGLIHGEFMPVGADGRIVLSFNLLNADPSDWTYDWDFRKIDETLLAPMIDAL 275

Query: 936  APLANISIESQVLYHTPKSSFAYWDKEKDSYIFSTKDLPFFVNSNEWHLDTSIAAGGRSK 757
             P+ANIS+ESQVLYHTPK S + WD++   YIFSTKDLPFFVNSNEWHLDTSIAAGGRSK
Sbjct: 276  GPIANISVESQVLYHTPKFSVSSWDEKLGGYIFSTKDLPFFVNSNEWHLDTSIAAGGRSK 335

Query: 756  ILQFVVYVPSAEECPLLLQLPNGEISETNGFISPMWGGVIVWNPPGCVRDGQN-LHLRKK 580
            ILQFVVYVPSA+ECPLLLQLPNGEIS+TN FISPMWGGV+VWNP  C RD  + L +R  
Sbjct: 336  ILQFVVYVPSAKECPLLLQLPNGEISKTNAFISPMWGGVMVWNPQSCSRDSDSELLVRHI 395

Query: 579  MSLQDLEKVFEVFVGQLRQLFGLNSGSLYRDASKTFTLLSSGKGITNWELDFLSRQHTCY 400
            MS +DL+KVFEVFVGQ RQLFGL SGSL+  A  T++LL+S KG T WELD LSRQHTC+
Sbjct: 396  MSPEDLQKVFEVFVGQFRQLFGLKSGSLHVGAMGTYSLLASEKGFTEWELDVLSRQHTCF 455

Query: 399  NLLQCGTTLGSLSRLVQSLPRMIIKDEIGKQVKFSLEAAKLARNNASYGIYDASAVSSRE 220
            N+    TTLGSLS+LVQSLPRMII DEIGKQVKFSLEAAKLAR NAS G YDASAVSSR+
Sbjct: 456  NIHSSATTLGSLSKLVQSLPRMIIMDEIGKQVKFSLEAAKLARVNASLGFYDASAVSSRQ 515

Query: 219  ARILAEDAFFHPSMMSVSYYSFEHCFAVYSPFFLPVSMHILLAVLREFRRYKQESAKHRA 40
            AR LAEDAFFHPS+MSVSYYSFEHCFAVYSPFFLPVSMH+LLA LRE+RRYKQE AK+  
Sbjct: 516  ARSLAEDAFFHPSIMSVSYYSFEHCFAVYSPFFLPVSMHVLLAALREWRRYKQEKAKYLL 575

Query: 39   WRATER 22
            W+A E+
Sbjct: 576  WKAKEK 581


>ref|XP_004160758.1| PREDICTED: GPI transamidase component PIG-S-like [Cucumis sativus]
          Length = 630

 Score =  646 bits (1666), Expect = 0.0
 Identities = 321/534 (60%), Positives = 401/534 (75%), Gaps = 23/534 (4%)
 Frame = -1

Query: 1563 VFVDFDYSEVSADDLGFSILGHMRKLTNNDHVCGNCGNNYSVSVTMESGGDCKQSESSDL 1384
            +F  FD     A+ L  SIL  M KL++   +CG+C NNY+VSV +ESG DC Q+ +   
Sbjct: 94   IFFGFDSMASRAEQLKSSILDEMTKLSSKSSLCGSCSNNYAVSVVIESGSDCSQTRTDAS 153

Query: 1383 SGLWQCGRIKGSNLDEVVKND-ESFDEYLYSMLGGSDEGSGNSGKVYSVVVLRRDDNIRA 1207
            S  W+CG +  S+    ++N  +S D++L   LGG  + + + G+VYSVVV+ + +N++A
Sbjct: 154  SCSWRCGALSASDFAASLENGLQSADDFLEVALGGCYKPA-SGGRVYSVVVMNKGENVKA 212

Query: 1206 VIGKNRHAWIVGRVLEMDATVEMVAEVFVKIFVNGGVRSGSIPAEFMPVGADGKIVLSFN 1027
             IGK RH WIVGRV E +A +  VAE FVK+F NGG   G IP EFMPVGADGKI LSFN
Sbjct: 213  TIGKYRHGWIVGRVSEAEA-IAKVAETFVKLFGNGGTEDGLIPGEFMPVGADGKIFLSFN 271

Query: 1026 LLNADPRDWIYDWSFQHIDDTLLAPIVKTLAPLANISIESQVLYHTPKSSFAYWDKEKDS 847
            LLNADP DWIYDW FQ +D+ +L P+++ L P+AN+S+ESQVLYHTP SSF+YWD +++S
Sbjct: 272  LLNADPDDWIYDWDFQKVDEVILKPLIEELTPIANVSVESQVLYHTPTSSFSYWDNKQES 331

Query: 846  YIFSTKDLPFF---------------------VNSNEWHLDTSIAAGGRSKILQFVVYVP 730
            YIF+TKDLPF                      VNSNEWHLDTSIAAGGRSKIL FVVY+P
Sbjct: 332  YIFNTKDLPFLLNVFVIRIEFISVSICLLTLPVNSNEWHLDTSIAAGGRSKILHFVVYIP 391

Query: 729  SAEECPLLLQLPNGEISETNGFISPMWGGVIVWNPPGCVRDGQNLHLRKKMSLQ-DLEKV 553
            SA ECPLLLQLP+G+ISETNGFISP WGGVIVWNP GC+RD ++  L + M L  +LEK+
Sbjct: 392  SARECPLLLQLPDGQISETNGFISPTWGGVIVWNPKGCLRDHESKLLHRHMILYPELEKI 451

Query: 552  FEVFVGQLRQLFGLNSGSLYRDASKTFTLLSSGKGITNWELDFLSRQHTCYNLLQCGTTL 373
             EVF+GQ RQLFGL S   +   S TF +L+S KG T WE+DFLSRQH+C+NL  C ++L
Sbjct: 452  VEVFLGQFRQLFGLKSNPQHVGLSGTFNILTSQKGFTEWEMDFLSRQHSCFNLHSCASSL 511

Query: 372  GSLSRLVQSLPRMIIKDEIGKQVKFSLEAAKLARNNASYGIYDASAVSSREARILAEDAF 193
            GSLSRLVQSLPRMII DEIGKQVK+SLEAA LA+ NAS G++DA+A+SSR+AR LAEDAF
Sbjct: 512  GSLSRLVQSLPRMIIIDEIGKQVKYSLEAANLAQKNASMGVFDAAAISSRQARSLAEDAF 571

Query: 192  FHPSMMSVSYYSFEHCFAVYSPFFLPVSMHILLAVLREFRRYKQESAKHRAWRA 31
            FHPS+MSVSY+SFEHCFAV SPFFLPV++H++LA +RE++RYKQE  K+ A+ A
Sbjct: 572  FHPSIMSVSYFSFEHCFAVNSPFFLPVALHVILAAVREWKRYKQEHKKYIAFLA 625