BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00010554
         (1572 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002277955.1| PREDICTED: tRNA-dihydrouridine synthase A-li...   383   e-165
ref|XP_002517816.1| tRNA-dihydrouridine synthase A, putative [Ri...   398   e-162
gb|AFK41814.1| unknown [Lotus japonicus]                              378   e-161
ref|XP_004165383.1| PREDICTED: tRNA-dihydrouridine synthase A-li...   382   e-161
ref|XP_004149715.1| PREDICTED: tRNA-dihydrouridine synthase A-li...   382   e-161

>ref|XP_002277955.1| PREDICTED: tRNA-dihydrouridine synthase A-like isoform 1 [Vitis
           vinifera]
          Length = 433

 Score =  383 bits (984), Expect(2) = e-165
 Identities = 191/258 (74%), Positives = 213/258 (82%)
 Frame = -1

Query: 882 KLVAEAMSVIAANCDVPVSVKCRIGVDDHDSYDELCDFIYEVSSHSPTKHFIIHSRKALL 703
           K VAEAMSVIAAN DVPVSVKCRIGVDDHDSY+ELCDFIY+VSS SPT+HFIIHSRKALL
Sbjct: 176 KFVAEAMSVIAANSDVPVSVKCRIGVDDHDSYNELCDFIYKVSSQSPTRHFIIHSRKALL 235

Query: 702 NGISPADNRKIPPLKYEFYYALLRDFPDLMFTINGGIKTIDEVNAARTEGAHGVMVGRAA 523
           NGISPADNRKIPPLKYE+Y+ LLRDFPDL FTINGGI  +DEVNAA  +GAHGVMVGRAA
Sbjct: 236 NGISPADNRKIPPLKYEYYFGLLRDFPDLRFTINGGINCVDEVNAALRQGAHGVMVGRAA 295

Query: 522 YNTPWTTLGLVDTAVYGAADNGLTRRQVLEQYQVYGDSVLGKQGNNRPNIRDIVKPTLGL 343
           Y+ PW TLG VD+AVYGA  + L+RRQVLEQYQVYGDSVL K G +RP+IR++VKP L  
Sbjct: 296 YHNPWYTLGHVDSAVYGAPVSDLSRRQVLEQYQVYGDSVLEKYGPHRPSIREVVKPLLHF 355

Query: 342 FHSAPGNGLWKRKADVALRRCTTIKSFFEDTLDAIPDWVLDEPLAETPSSSVETFANSKT 163
           F+S PGNGLWKRKAD A   C TIKSF E+TL AIPD VLD P+A+ PS   + FAN   
Sbjct: 356 FYSEPGNGLWKRKADAAFFHCKTIKSFLEETLVAIPDSVLDAPVAKAPSGREDLFANLSD 415

Query: 162 LLPPPYTYTQSEQIVLSA 109
           LLP PY  ++ EQ VL A
Sbjct: 416 LLPLPYETSEQEQEVLYA 433



 Score =  227 bits (578), Expect(2) = e-165
 Identities = 112/175 (64%), Positives = 132/175 (75%), Gaps = 5/175 (2%)
 Frame = -2

Query: 1412 MTAAGYIWSVSYTPVYCTILGRSVRLSQNFVC--STRSQRLRTYFQNHSQHTAPVQ---R 1248
            M +AGY  + S+ P++  IL    RLS N +   S +S+    Y + H  HT  V    +
Sbjct: 2    MKSAGYTLTASFNPIHSIILKNHRRLSLNILYGNSAKSKNSTAYIRKHQLHTKVVMVAGQ 61

Query: 1247 HLSPLFSVAPMMDWTDNHYRTLARLISKHAWLYTEMVAAETIVHQTENLDRFLAYTPEQH 1068
            +L P FSVAPMMDWT+NHYRTLARLISKHAWLYTEM+AAETIV+Q +NLDRFL ++P+QH
Sbjct: 62   YLPPWFSVAPMMDWTNNHYRTLARLISKHAWLYTEMIAAETIVYQEKNLDRFLEFSPDQH 121

Query: 1067 PIVLQIGGNKLDRLAKAVELARPYGYDEINLNCGCPSSKVAGHGCFGVSLMLEPK 903
            PIVLQIGGN LD +AKA ELA  Y YDEINLNCGCPS KVAGHGCFGV LML+PK
Sbjct: 122  PIVLQIGGNNLDNIAKATELANSYCYDEINLNCGCPSPKVAGHGCFGVRLMLDPK 176


>ref|XP_002517816.1| tRNA-dihydrouridine synthase A, putative [Ricinus communis]
           gi|223543088|gb|EEF44623.1| tRNA-dihydrouridine synthase
           A, putative [Ricinus communis]
          Length = 375

 Score =  398 bits (1023), Expect(2) = e-162
 Identities = 190/252 (75%), Positives = 214/252 (84%)
 Frame = -1

Query: 882 KLVAEAMSVIAANCDVPVSVKCRIGVDDHDSYDELCDFIYEVSSHSPTKHFIIHSRKALL 703
           K V EAMSVIAAN DVPVSVKCRIGVD+HDSY+ELCDFIY+VSS SPTKHFIIHSRKALL
Sbjct: 120 KFVGEAMSVIAANTDVPVSVKCRIGVDNHDSYNELCDFIYKVSSLSPTKHFIIHSRKALL 179

Query: 702 NGISPADNRKIPPLKYEFYYALLRDFPDLMFTINGGIKTIDEVNAARTEGAHGVMVGRAA 523
           NGISPA+NRKIPPLKYE+Y+ALLRDFPDL FTINGGI  IDEVN A  EGAHGVMVGRAA
Sbjct: 180 NGISPAENRKIPPLKYEYYFALLRDFPDLRFTINGGINCIDEVNLALREGAHGVMVGRAA 239

Query: 522 YNTPWTTLGLVDTAVYGAADNGLTRRQVLEQYQVYGDSVLGKQGNNRPNIRDIVKPTLGL 343
           +N PWT LG VD+A+YGA ++GLTRRQVLEQYQ Y D++LG  GNNRPN+RD+VKP LGL
Sbjct: 240 FNNPWTVLGHVDSAIYGAPESGLTRRQVLEQYQAYADAILGTYGNNRPNVRDVVKPLLGL 299

Query: 342 FHSAPGNGLWKRKADVALRRCTTIKSFFEDTLDAIPDWVLDEPLAETPSSSVETFANSKT 163
           F+S PGNGLWKR+AD A + CTTIKSFFE+TL AIPD VLD P+AE PS   + F N + 
Sbjct: 300 FYSEPGNGLWKRRADAAFQSCTTIKSFFEETLVAIPDTVLDSPIAEAPSGRQDLFTNVRG 359

Query: 162 LLPPPYTYTQSE 127
           LLPPPY   + +
Sbjct: 360 LLPPPYATREQD 371



 Score =  203 bits (516), Expect(2) = e-162
 Identities = 95/121 (78%), Positives = 106/121 (87%)
 Frame = -2

Query: 1256 VQRHLSPLFSVAPMMDWTDNHYRTLARLISKHAWLYTEMVAAETIVHQTENLDRFLAYTP 1077
            V R+  PLFSVAPMMDWTD+HYRTLARLISKH+WLYTEM+AAETIV+Q  NLDRFLA++P
Sbjct: 3    VGRYHPPLFSVAPMMDWTDHHYRTLARLISKHSWLYTEMLAAETIVYQQGNLDRFLAFSP 62

Query: 1076 EQHPIVLQIGGNKLDRLAKAVELARPYGYDEINLNCGCPSSKVAGHGCFGVSLMLEPKKC 897
            +QHPIVLQIGG+ LD LAKA ELA  YGYDEIN NCGCPS +VAGHGCFGV LML+PK  
Sbjct: 63   QQHPIVLQIGGSNLDNLAKATELATAYGYDEINFNCGCPSPRVAGHGCFGVRLMLDPKFV 122

Query: 896  G 894
            G
Sbjct: 123  G 123


>gb|AFK41814.1| unknown [Lotus japonicus]
          Length = 425

 Score =  378 bits (970), Expect(2) = e-161
 Identities = 189/258 (73%), Positives = 209/258 (81%)
 Frame = -1

Query: 882 KLVAEAMSVIAANCDVPVSVKCRIGVDDHDSYDELCDFIYEVSSHSPTKHFIIHSRKALL 703
           K VAEAMS I AN +VPVSVKCRIGVDDHDSYDELCDFI++VSS SPTKHFIIHSRKALL
Sbjct: 170 KFVAEAMSAITANTNVPVSVKCRIGVDDHDSYDELCDFIFKVSSLSPTKHFIIHSRKALL 229

Query: 702 NGISPADNRKIPPLKYEFYYALLRDFPDLMFTINGGIKTIDEVNAARTEGAHGVMVGRAA 523
           NGISPA+NR IPPLKYE++Y LLRDFPDL FTINGGI ++DEV AAR  GA+GV VGRAA
Sbjct: 230 NGISPAENRSIPPLKYEYFYGLLRDFPDLTFTINGGITSVDEVYAAREAGAYGVTVGRAA 289

Query: 522 YNTPWTTLGLVDTAVYGAADNGLTRRQVLEQYQVYGDSVLGKQGNNRPNIRDIVKPTLGL 343
           YN PW  LG VDT +YGA    LTRRQVLE+Y VYGDSVLGK G  RP +RDIVKP L L
Sbjct: 290 YNNPWHILGHVDTVIYGAPSCSLTRRQVLEKYIVYGDSVLGKYG-RRPTVRDIVKPLLNL 348

Query: 342 FHSAPGNGLWKRKADVALRRCTTIKSFFEDTLDAIPDWVLDEPLAETPSSSVETFANSKT 163
           FHSAPGNGLWKRKAD A R CTTIKSFFE+TL AIPD VLD P+ E      + FAN  +
Sbjct: 349 FHSAPGNGLWKRKADAAFRHCTTIKSFFEETLVAIPDSVLDSPVVEPTPGRRDLFANMDS 408

Query: 162 LLPPPYTYTQSEQIVLSA 109
           LLPPPYT T+ E +++ A
Sbjct: 409 LLPPPYT-TREEAVIICA 425



 Score =  219 bits (557), Expect(2) = e-161
 Identities = 109/172 (63%), Positives = 136/172 (79%), Gaps = 2/172 (1%)
 Frame = -2

Query: 1412 MTAAGYIWSVSYTPVYCTILGRSVR--LSQNFVCSTRSQRLRTYFQNHSQHTAPVQRHLS 1239
            M  +GY  +VS++PV  T++  + R  + +NF  S+R    +   Q+H +   P   +L 
Sbjct: 2    MKFSGYSLAVSFSPVGSTVIRNNCRFHVGRNFRSSSRFFSAKQ--QSHDKGVLP-NYYLP 58

Query: 1238 PLFSVAPMMDWTDNHYRTLARLISKHAWLYTEMVAAETIVHQTENLDRFLAYTPEQHPIV 1059
            P FSVAPMM+WTD+HYRTLARLISKHAWLYTEM+AAETIV+Q +NLDRFLA++P+QHPIV
Sbjct: 59   PWFSVAPMMEWTDHHYRTLARLISKHAWLYTEMLAAETIVYQQDNLDRFLAFSPDQHPIV 118

Query: 1058 LQIGGNKLDRLAKAVELARPYGYDEINLNCGCPSSKVAGHGCFGVSLMLEPK 903
            LQIGG+ +++LAKA ELA  YGYDEINLNCGCPS KVAGHGCFGVSLML+PK
Sbjct: 119  LQIGGSNVEKLAKATELANAYGYDEINLNCGCPSPKVAGHGCFGVSLMLDPK 170


>ref|XP_004165383.1| PREDICTED: tRNA-dihydrouridine synthase A-like [Cucumis sativus]
          Length = 431

 Score =  382 bits (982), Expect(2) = e-161
 Identities = 186/258 (72%), Positives = 215/258 (83%)
 Frame = -1

Query: 882 KLVAEAMSVIAANCDVPVSVKCRIGVDDHDSYDELCDFIYEVSSHSPTKHFIIHSRKALL 703
           K V EAMSVIAA  D PVSVKCRIGVDDHDSY+ELCDFIY+VSS SPTKHF+IHSRKALL
Sbjct: 176 KFVGEAMSVIAAKTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFVIHSRKALL 235

Query: 702 NGISPADNRKIPPLKYEFYYALLRDFPDLMFTINGGIKTIDEVNAARTEGAHGVMVGRAA 523
           NGISPA+NR IPPLKYE++YAL+RDFPDL FTINGGI T+DE NAA   GAHGVM+GRAA
Sbjct: 236 NGISPAENRTIPPLKYEYFYALMRDFPDLRFTINGGINTVDEANAALRLGAHGVMMGRAA 295

Query: 522 YNTPWTTLGLVDTAVYGAADNGLTRRQVLEQYQVYGDSVLGKQGNNRPNIRDIVKPTLGL 343
           Y  PW TLG VDTA+YGA  +G+TRRQVLEQYQVYGDSVLG+ G N+PNIRD+VKP L L
Sbjct: 296 YQNPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGRYG-NKPNIRDVVKPLLHL 354

Query: 342 FHSAPGNGLWKRKADVALRRCTTIKSFFEDTLDAIPDWVLDEPLAETPSSSVETFANSKT 163
           F++ PGNG WKRKAD A   C TIKSFFE+T+ AIPD+VLD P+AE PS   + FAN+ +
Sbjct: 355 FYTDPGNGPWKRKADAAFMHCKTIKSFFEETIVAIPDYVLDAPVAEPPSGREDLFANTLS 414

Query: 162 LLPPPYTYTQSEQIVLSA 109
           L+PPPY   + +++VL A
Sbjct: 415 LMPPPYE-DREQKVVLEA 431



 Score =  213 bits (543), Expect(2) = e-161
 Identities = 110/178 (61%), Positives = 126/178 (70%), Gaps = 5/178 (2%)
 Frame = -2

Query: 1412 MTAAGYIWSVSYTPVYCTILGRSVRLSQNFVCSTRSQRLRTYFQNHSQHTAP-----VQR 1248
            M  AG   + S TPV   I     RLS + +    S+ +     N      P       R
Sbjct: 2    MKFAGCSVTASLTPVQSVIQNSHPRLSSSILQKNLSKPISWSSGNQILRQGPEAEMVAGR 61

Query: 1247 HLSPLFSVAPMMDWTDNHYRTLARLISKHAWLYTEMVAAETIVHQTENLDRFLAYTPEQH 1068
            +  P FSVAPMM+ TDNHYRTLARLISKHAWLYTEM+AAETIV+Q +NLDRFLA++P+QH
Sbjct: 62   YSPPWFSVAPMMECTDNHYRTLARLISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPDQH 121

Query: 1067 PIVLQIGGNKLDRLAKAVELARPYGYDEINLNCGCPSSKVAGHGCFGVSLMLEPKKCG 894
            PIVLQIGGN LD +AKA+ELA PYGYDEINLNCGCPS KVAGHGCFGV LML+PK  G
Sbjct: 122  PIVLQIGGNNLDNIAKAIELANPYGYDEINLNCGCPSPKVAGHGCFGVRLMLDPKFVG 179


>ref|XP_004149715.1| PREDICTED: tRNA-dihydrouridine synthase A-like [Cucumis sativus]
          Length = 431

 Score =  382 bits (982), Expect(2) = e-161
 Identities = 186/258 (72%), Positives = 215/258 (83%)
 Frame = -1

Query: 882 KLVAEAMSVIAANCDVPVSVKCRIGVDDHDSYDELCDFIYEVSSHSPTKHFIIHSRKALL 703
           K V EAMSVIAA  D PVSVKCRIGVDDHDSY+ELCDFIY+VSS SPTKHF+IHSRKALL
Sbjct: 176 KFVGEAMSVIAAKTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFVIHSRKALL 235

Query: 702 NGISPADNRKIPPLKYEFYYALLRDFPDLMFTINGGIKTIDEVNAARTEGAHGVMVGRAA 523
           NGISPA+NR IPPLKYE++YAL+RDFPDL FTINGGI T+DE NAA   GAHGVM+GRAA
Sbjct: 236 NGISPAENRTIPPLKYEYFYALMRDFPDLRFTINGGINTVDEANAALRLGAHGVMMGRAA 295

Query: 522 YNTPWTTLGLVDTAVYGAADNGLTRRQVLEQYQVYGDSVLGKQGNNRPNIRDIVKPTLGL 343
           Y  PW TLG VDTA+YGA  +G+TRRQVLEQYQVYGDSVLG+ G N+PNIRD+VKP L L
Sbjct: 296 YQNPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGRYG-NKPNIRDVVKPLLHL 354

Query: 342 FHSAPGNGLWKRKADVALRRCTTIKSFFEDTLDAIPDWVLDEPLAETPSSSVETFANSKT 163
           F++ PGNG WKRKAD A   C TIKSFFE+T+ AIPD+VLD P+AE PS   + FAN+ +
Sbjct: 355 FYTDPGNGPWKRKADAAFMHCKTIKSFFEETIVAIPDYVLDAPVAEPPSGREDLFANTLS 414

Query: 162 LLPPPYTYTQSEQIVLSA 109
           L+PPPY   + +++VL A
Sbjct: 415 LMPPPYE-DREQKVVLEA 431



 Score =  213 bits (542), Expect(2) = e-161
 Identities = 110/178 (61%), Positives = 126/178 (70%), Gaps = 5/178 (2%)
 Frame = -2

Query: 1412 MTAAGYIWSVSYTPVYCTILGRSVRLSQNFVCSTRSQRLRTYFQNHSQHTAP-----VQR 1248
            M  AG   + S TPV   I     RLS + +    S+ +     N      P       R
Sbjct: 2    MKFAGCSVTASSTPVQSVIQNSHPRLSSSILQKNLSKPISWSSGNQILRQGPEAEMVAGR 61

Query: 1247 HLSPLFSVAPMMDWTDNHYRTLARLISKHAWLYTEMVAAETIVHQTENLDRFLAYTPEQH 1068
            +  P FSVAPMM+ TDNHYRTLARLISKHAWLYTEM+AAETIV+Q +NLDRFLA++P+QH
Sbjct: 62   YSPPWFSVAPMMECTDNHYRTLARLISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPDQH 121

Query: 1067 PIVLQIGGNKLDRLAKAVELARPYGYDEINLNCGCPSSKVAGHGCFGVSLMLEPKKCG 894
            PIVLQIGGN LD +AKA+ELA PYGYDEINLNCGCPS KVAGHGCFGV LML+PK  G
Sbjct: 122  PIVLQIGGNNLDNIAKAIELANPYGYDEINLNCGCPSPKVAGHGCFGVRLMLDPKFVG 179