BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00010553
         (1067 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002309233.1| predicted protein [Populus trichocarpa] gi|2...   263   e-140
ref|XP_002283565.2| PREDICTED: probable glutamate carboxypeptida...   268   e-138
ref|XP_003517636.1| PREDICTED: probable glutamate carboxypeptida...   259   e-137
ref|XP_003633117.1| PREDICTED: probable glutamate carboxypeptida...   251   e-133
ref|XP_002871904.1| peptidase M28 family protein [Arabidopsis ly...   258   e-126

>ref|XP_002309233.1| predicted protein [Populus trichocarpa] gi|222855209|gb|EEE92756.1|
           predicted protein [Populus trichocarpa]
          Length = 709

 Score =  263 bits (673), Expect(2) = e-140
 Identities = 121/159 (76%), Positives = 137/159 (86%)
 Frame = +1

Query: 1   GGADAKGFPYDKWMPPSGVQVGSLYKGAGDPTTPGWPSTKECERISDNEVDKGGDIPQIP 180
           GG D   FP  KWMPPSGVQVGS+Y GAGDP+TPGWPS + CERISD+EV+K G++P IP
Sbjct: 213 GGGDEGWFPKAKWMPPSGVQVGSVYDGAGDPSTPGWPSIQGCERISDDEVEKQGNVPLIP 272

Query: 181 SLPVSAVDGEAIIKSIGGEVADEDWQGCKDGPVYSIGPGPGILNLSYTGKQVINTIENVI 360
           SLPVSA DGE I++S+GG+VA+EDWQG KD P Y +GPGPGILNL+YTGK+ I TI+NVI
Sbjct: 273 SLPVSAADGETIMRSVGGQVANEDWQGSKDSPTYRLGPGPGILNLTYTGKKAIETIQNVI 332

Query: 361 GIIEGAEEPDRFVILGNHRDAWTFGAADPNSGTAALLEV 477
            IIEG EEPDRFVILGNHRDAWTFGA DPNSGTAALLEV
Sbjct: 333 AIIEGVEEPDRFVILGNHRDAWTFGAVDPNSGTAALLEV 371



 Score =  261 bits (666), Expect(2) = e-140
 Identities = 121/181 (66%), Positives = 137/181 (75%)
 Frame = +3

Query: 522  RLRKLQKKGWKPRRTIIFCNWDAEEYGLIGSTEWVEENREMLASRVVAYLNXXXXXXXXX 701
            RL KLQ+KGWKPRRTI+ CNWDAEEYGLIGSTEWVE+NRE+L SR VAYLN         
Sbjct: 374  RLMKLQEKGWKPRRTIVLCNWDAEEYGLIGSTEWVEDNRELLTSRAVAYLNVDCAVTGAG 433

Query: 702  XXXXXTPXXXXXXXXXXXXXXXPDNSSQSIFDSWVGNSDHPKMGRLGGGGSDYAAFVQHV 881
                 TP               PDNSSQ+I++SWV   + P + RLGGGGSDYAAFVQH+
Sbjct: 434  FHASATPQLDKLLAKTTQQVGDPDNSSQTIYESWVAPDNSPTIERLGGGGSDYAAFVQHI 493

Query: 882  GIPSADMSFGEGYPVYHSMYDDFIWMRDFGDPMFRRHVAVASIWGLVALALADEEFLPFN 1061
            GI SAD+SFG+GYPVYHSMYDDF+WM  FGDPMFRRH+AVAS+WGL AL LADEEFLPF+
Sbjct: 494  GIASADVSFGKGYPVYHSMYDDFVWMEKFGDPMFRRHIAVASVWGLTALQLADEEFLPFD 553

Query: 1062 Y 1064
            Y
Sbjct: 554  Y 554


>ref|XP_002283565.2| PREDICTED: probable glutamate carboxypeptidase 2-like isoform 1
            [Vitis vinifera]
          Length = 704

 Score =  268 bits (684), Expect(2) = e-138
 Identities = 124/181 (68%), Positives = 140/181 (77%)
 Frame = +3

Query: 522  RLRKLQKKGWKPRRTIIFCNWDAEEYGLIGSTEWVEENREMLASRVVAYLNXXXXXXXXX 701
            RLRKLQK+GW+PRRTI+ CNWDAEEYGL GSTEWVEENREMLAS+ +AYLN         
Sbjct: 369  RLRKLQKRGWRPRRTIVLCNWDAEEYGLTGSTEWVEENREMLASKAIAYLNVDSAVSGAG 428

Query: 702  XXXXXTPXXXXXXXXXXXXXXXPDNSSQSIFDSWVGNSDHPKMGRLGGGGSDYAAFVQHV 881
                 TP               PDNSSQSI++SWVG+S+ P +GRLGGGGSD+AAFVQHV
Sbjct: 429  FYASATPQLDELLKQATQQVQDPDNSSQSIYESWVGSSNSPIIGRLGGGGSDFAAFVQHV 488

Query: 882  GIPSADMSFGEGYPVYHSMYDDFIWMRDFGDPMFRRHVAVASIWGLVALALADEEFLPFN 1061
            G+P+ D+SFG GYPVYHSMYDDFIWM+ FGDPMF RH A ASIWGLVAL LADEEFLPF+
Sbjct: 489  GVPATDISFGAGYPVYHSMYDDFIWMKKFGDPMFHRHAAAASIWGLVALRLADEEFLPFD 548

Query: 1062 Y 1064
            Y
Sbjct: 549  Y 549



 Score =  251 bits (641), Expect(2) = e-138
 Identities = 116/160 (72%), Positives = 134/160 (83%), Gaps = 1/160 (0%)
 Frame = +1

Query: 1   GGADAKGFPYDKWMPPSGVQVGSLYKGAGDPTTPGWPSTK-ECERISDNEVDKGGDIPQI 177
           GG DA+ FP DKWMPPSGVQVGSL    GDPTTPGW ST+ ECER+S+ EV+ GG +  I
Sbjct: 207 GGGDARWFPDDKWMPPSGVQVGSLLTVDGDPTTPGWASTRDECERLSEEEVENGGGVGMI 266

Query: 178 PSLPVSAVDGEAIIKSIGGEVADEDWQGCKDGPVYSIGPGPGILNLSYTGKQVINTIENV 357
           PSLP+SA DGEAI+++IGG+VA++DWQG  D PVY +GPGP ILNLSYTG+Q I TI+NV
Sbjct: 267 PSLPISAADGEAIMRTIGGQVANDDWQGSTDAPVYRVGPGPAILNLSYTGEQKITTIQNV 326

Query: 358 IGIIEGAEEPDRFVILGNHRDAWTFGAADPNSGTAALLEV 477
            G+IEG EEPDRFV+LGNHRDAWTFGA DPNSGTA LLEV
Sbjct: 327 FGVIEGTEEPDRFVLLGNHRDAWTFGAVDPNSGTATLLEV 366


>ref|XP_003517636.1| PREDICTED: probable glutamate carboxypeptidase 2-like [Glycine max]
          Length = 693

 Score =  259 bits (661), Expect(2) = e-137
 Identities = 120/181 (66%), Positives = 137/181 (75%)
 Frame = +3

Query: 522  RLRKLQKKGWKPRRTIIFCNWDAEEYGLIGSTEWVEENREMLASRVVAYLNXXXXXXXXX 701
            RL KLQKKGW+PRRTI+ CNWDAEEYGLIGSTEWVEENRE+LAS+ VAYLN         
Sbjct: 371  RLGKLQKKGWRPRRTILLCNWDAEEYGLIGSTEWVEENREILASKAVAYLNADCVVGGPG 430

Query: 702  XXXXXTPXXXXXXXXXXXXXXXPDNSSQSIFDSWVGNSDHPKMGRLGGGGSDYAAFVQHV 881
                 TP               PDNSSQSI++SW  +   P  GRLGGGGSDYA+F+QHV
Sbjct: 431  FNVRATPQLDELIKRATQEVKDPDNSSQSIYESWTSSGSSPLFGRLGGGGSDYASFLQHV 490

Query: 882  GIPSADMSFGEGYPVYHSMYDDFIWMRDFGDPMFRRHVAVASIWGLVALALADEEFLPFN 1061
            GIP+AD++FG GYPVYHS+YDDF+WM  FGDPMF+RHVA AS+WGLVAL LADEEFLPF+
Sbjct: 491  GIPAADIAFGGGYPVYHSLYDDFVWMEKFGDPMFQRHVAAASVWGLVALWLADEEFLPFD 550

Query: 1062 Y 1064
            Y
Sbjct: 551  Y 551



 Score =  255 bits (652), Expect(2) = e-137
 Identities = 118/162 (72%), Positives = 138/162 (85%), Gaps = 4/162 (2%)
 Frame = +1

Query: 4   GADAKGFPYDKWMPPSGVQVGSLYKGAGDPTTPGWPSTK----ECERISDNEVDKGGDIP 171
           G + K FP +KW+PPSGVQVG++Y G GDPTTPGW S+     ECER++ +EV+KGGD+P
Sbjct: 207 GGEEKWFPDEKWLPPSGVQVGTVYGGLGDPTTPGWASSSSGDGECERLNKDEVEKGGDVP 266

Query: 172 QIPSLPVSAVDGEAIIKSIGGEVADEDWQGCKDGPVYSIGPGPGILNLSYTGKQVINTIE 351
            IPSLPVSA DGE I++SIGG VA++DWQG KD P+Y +GPGPGILNLSY G+ VI TI+
Sbjct: 267 LIPSLPVSAADGEKIMRSIGGPVAEDDWQGSKDAPIYRLGPGPGILNLSYKGQDVIATIQ 326

Query: 352 NVIGIIEGAEEPDRFVILGNHRDAWTFGAADPNSGTAALLEV 477
           NVIG+IEGAEEPDRFVILGNHRDAWTFGA DPNSGTAALLEV
Sbjct: 327 NVIGVIEGAEEPDRFVILGNHRDAWTFGAVDPNSGTAALLEV 368


>ref|XP_003633117.1| PREDICTED: probable glutamate carboxypeptidase 2-like isoform 2
            [Vitis vinifera]
          Length = 689

 Score =  251 bits (642), Expect(2) = e-133
 Identities = 122/187 (65%), Positives = 138/187 (73%), Gaps = 6/187 (3%)
 Frame = +3

Query: 522  RLRKLQKKGWKPRRTIIFCNWDAEEYGLIGSTEWVEENREMLASRVVAYLNXXXXXXXXX 701
            RLRKLQK+GW+PRRTI+ CNWDAEEYGL GSTEWVEENREMLAS+ +AYLN         
Sbjct: 369  RLRKLQKRGWRPRRTIVLCNWDAEEYGLTGSTEWVEENREMLASKAIAYLNVDSAVSGVQ 428

Query: 702  XXXXXTPXXXXXXXXXXXXXXXPDNSSQSIFDSWVGNSDHPKMGRLGGGGSDYAAFVQHV 881
                                  PDNSSQSI++SWVG+S+ P +GRLGGGGSD+AAFVQHV
Sbjct: 429  D---------------------PDNSSQSIYESWVGSSNSPIIGRLGGGGSDFAAFVQHV 467

Query: 882  GIPSADMSFGEG------YPVYHSMYDDFIWMRDFGDPMFRRHVAVASIWGLVALALADE 1043
            G+P+ D+SFG G      YPVYHSMYDDFIWM+ FGDPMF RH A ASIWGLVAL LADE
Sbjct: 468  GVPATDISFGAGVFITAGYPVYHSMYDDFIWMKKFGDPMFHRHAAAASIWGLVALRLADE 527

Query: 1044 EFLPFNY 1064
            EFLPF+Y
Sbjct: 528  EFLPFDY 534



 Score =  251 bits (641), Expect(2) = e-133
 Identities = 116/160 (72%), Positives = 134/160 (83%), Gaps = 1/160 (0%)
 Frame = +1

Query: 1   GGADAKGFPYDKWMPPSGVQVGSLYKGAGDPTTPGWPSTK-ECERISDNEVDKGGDIPQI 177
           GG DA+ FP DKWMPPSGVQVGSL    GDPTTPGW ST+ ECER+S+ EV+ GG +  I
Sbjct: 207 GGGDARWFPDDKWMPPSGVQVGSLLTVDGDPTTPGWASTRDECERLSEEEVENGGGVGMI 266

Query: 178 PSLPVSAVDGEAIIKSIGGEVADEDWQGCKDGPVYSIGPGPGILNLSYTGKQVINTIENV 357
           PSLP+SA DGEAI+++IGG+VA++DWQG  D PVY +GPGP ILNLSYTG+Q I TI+NV
Sbjct: 267 PSLPISAADGEAIMRTIGGQVANDDWQGSTDAPVYRVGPGPAILNLSYTGEQKITTIQNV 326

Query: 358 IGIIEGAEEPDRFVILGNHRDAWTFGAADPNSGTAALLEV 477
            G+IEG EEPDRFV+LGNHRDAWTFGA DPNSGTA LLEV
Sbjct: 327 FGVIEGTEEPDRFVLLGNHRDAWTFGAVDPNSGTATLLEV 366


>ref|XP_002871904.1| peptidase M28 family protein [Arabidopsis lyrata subsp. lyrata]
            gi|297317741|gb|EFH48163.1| peptidase M28 family protein
            [Arabidopsis lyrata subsp. lyrata]
          Length = 682

 Score =  258 bits (660), Expect(2) = e-126
 Identities = 122/182 (67%), Positives = 137/182 (75%)
 Frame = +3

Query: 522  RLRKLQKKGWKPRRTIIFCNWDAEEYGLIGSTEWVEENREMLASRVVAYLNXXXXXXXXX 701
            RL KLQK+GWKPRRTII CNWDAEEYGLIGSTEWVEENREML SR VAYLN         
Sbjct: 356  RLDKLQKRGWKPRRTIILCNWDAEEYGLIGSTEWVEENREMLTSRAVAYLNVDCAVSGPG 415

Query: 702  XXXXXTPXXXXXXXXXXXXXXXPDNSSQSIFDSWVGNSDHPKMGRLGGGGSDYAAFVQHV 881
                 TP               PDN++Q+I+DSW+G+S+   +GRLGGGGSDYA+FVQHV
Sbjct: 416  FHASATPQLDELIKIAAQEVRDPDNATQTIYDSWIGSSNSVVIGRLGGGGSDYASFVQHV 475

Query: 882  GIPSADMSFGEGYPVYHSMYDDFIWMRDFGDPMFRRHVAVASIWGLVALALADEEFLPFN 1061
            G+P  DMSFG GYPVYHSMYDDF WM  FGDPMF+RHVA+AS+ GLVAL LADEE LPFN
Sbjct: 476  GVPGVDMSFGGGYPVYHSMYDDFTWMEKFGDPMFQRHVAMASVLGLVALRLADEEILPFN 535

Query: 1062 YS 1067
            Y+
Sbjct: 536  YT 537



 Score =  219 bits (558), Expect(2) = e-126
 Identities = 103/152 (67%), Positives = 118/152 (77%)
 Frame = +1

Query: 22  FPYDKWMPPSGVQVGSLYKGAGDPTTPGWPSTKECERISDNEVDKGGDIPQIPSLPVSAV 201
           FP  KWMPPSGVQVG++Y G GDPTTPGW S   CER+SD  V+  GD+P IPSLPVSA 
Sbjct: 210 FPASKWMPPSGVQVGTVYNGLGDPTTPGWASVDGCERLSDEAVELSGDVPLIPSLPVSAA 269

Query: 202 DGEAIIKSIGGEVADEDWQGCKDGPVYSIGPGPGILNLSYTGKQVINTIENVIGIIEGAE 381
           D E I+K++ G+V D        G VY +GPGPGILNLSYTG+ VI  IENVIG+IEG E
Sbjct: 270 DAEVILKTVVGDVGD--------GDVYPVGPGPGILNLSYTGETVIAQIENVIGVIEGEE 321

Query: 382 EPDRFVILGNHRDAWTFGAADPNSGTAALLEV 477
           EPDR++ILGNHRDAWTFGA DPNSGTA LLE+
Sbjct: 322 EPDRYLILGNHRDAWTFGAVDPNSGTAVLLEI 353