BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00010328
         (1215 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]      474   e-131
gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudi...   474   e-131
ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P...   468   e-129
gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P...   467   e-129
ref|XP_003524231.1| PREDICTED: cytochrome P450 716B2-like [Glyci...   445   e-122

>gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]
          Length = 476

 Score =  474 bits (1221), Expect = e-131
 Identities = 221/339 (65%), Positives = 275/339 (81%)
 Frame = -2

Query: 1214 LSPDAFSRLYINIMDLVTQKHISTHWQGKQEVKVYNTIKLYTFELACRLFTSLEDPDHIK 1035
            L PDAF+  Y   MD+VT++HI  HW+GK+EV V+ T+KLY FELACRLF +L+DP+HI 
Sbjct: 136  LGPDAFATHYAVTMDVVTRRHIDVHWRGKEEVNVFQTVKLYAFELACRLFMNLDDPNHIA 195

Query: 1034 KLASQFNIFLKGIIQIPLNVPGTRFYYAMRAANAIRKDLLEITRQRRIDLEQKTASPSQD 855
            KL S FNIFLKGII++P++VPGTRFY + +AA AIR +L ++ + R+++L++   S SQD
Sbjct: 196  KLGSLFNIFLKGIIELPIDVPGTRFYSSKKAAAAIRIELKKLIKARKLELKEGKPSSSQD 255

Query: 854  LLSHLLVSCDEHGRSLTEDEIVNNILTLLFAGHDTSSVSITLAMKILAEKPQVYQKVLDE 675
            LLSHLL S DE+G  LTE+EIV+NIL LLFAGHDTS++SITL MK L E   VY KVL E
Sbjct: 256  LLSHLLTSPDENGMFLTEEEIVDNILLLLFAGHDTSALSITLLMKALGEHSDVYDKVLKE 315

Query: 674  HMDIAASKRKDELLQWDDIQKMKYTWNVVCEVMRLTPPVTGAFREALVEFDFAGYTIPKG 495
             ++I+  K   ELL+W+DIQKMKY+W+VVCEVMRL PPV GA+REALV+ D+AGYTIPKG
Sbjct: 316  QLEISKGKEAGELLKWEDIQKMKYSWSVVCEVMRLNPPVIGAYREALVDIDYAGYTIPKG 375

Query: 494  WKLYWSTVSTNKDDKYFPDPTEFDATRFEGAGPTPYSYVPFGGGPRMCLGKEFARMEILV 315
            WKL+WS VST +D+  F D T FD +RFEGAGPTP+++VPFGGGPRMCLGKEFAR+E+L 
Sbjct: 376  WKLHWSAVSTQRDEANFEDVTRFDPSRFEGAGPTPFTFVPFGGGPRMCLGKEFARLEVLA 435

Query: 314  FLHNVVKRFKWDLLIPNEKMEYDPMPIPVNGLPISLHPH 198
            FLHN+V  FKWDLLIP+EK+EYDP+  P  GLPI LHPH
Sbjct: 436  FLHNIVINFKWDLLIPDEKIEYDPIATPAKGLPIRLHPH 474


>gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudiana]
            gi|189418962|gb|ACD93722.1| ent-kaurenoic acid
            13-hydroxylase [Stevia rebaudiana]
          Length = 476

 Score =  474 bits (1219), Expect = e-131
 Identities = 220/339 (64%), Positives = 275/339 (81%)
 Frame = -2

Query: 1214 LSPDAFSRLYINIMDLVTQKHISTHWQGKQEVKVYNTIKLYTFELACRLFTSLEDPDHIK 1035
            L PDAF+  Y   MD+VT++HI  HW+GK+EV V+ T+KLY FELACRLF +L+DP+HI 
Sbjct: 136  LGPDAFATHYAVTMDVVTRRHIDVHWRGKEEVNVFQTVKLYAFELACRLFMNLDDPNHIA 195

Query: 1034 KLASQFNIFLKGIIQIPLNVPGTRFYYAMRAANAIRKDLLEITRQRRIDLEQKTASPSQD 855
            KL S FNIFLKGII++P++VPGTRFY + +AA AIR +L ++ + R+++L++  AS SQD
Sbjct: 196  KLGSLFNIFLKGIIELPIDVPGTRFYSSKKAAAAIRIELKKLIKARKLELKEGKASSSQD 255

Query: 854  LLSHLLVSCDEHGRSLTEDEIVNNILTLLFAGHDTSSVSITLAMKILAEKPQVYQKVLDE 675
            LLSHLL S DE+G  LTE+EIV+NIL LLFAGHDTS++SITL MK L E   VY KVL E
Sbjct: 256  LLSHLLTSPDENGMFLTEEEIVDNILLLLFAGHDTSALSITLLMKTLGEHSDVYDKVLKE 315

Query: 674  HMDIAASKRKDELLQWDDIQKMKYTWNVVCEVMRLTPPVTGAFREALVEFDFAGYTIPKG 495
             ++I+ +K   E L+W+DIQKMKY+W+V+CEVMRL PPV G +REALV+ D+AGYTIPKG
Sbjct: 316  QLEISKTKEAWESLKWEDIQKMKYSWSVICEVMRLNPPVIGTYREALVDIDYAGYTIPKG 375

Query: 494  WKLYWSTVSTNKDDKYFPDPTEFDATRFEGAGPTPYSYVPFGGGPRMCLGKEFARMEILV 315
            WKL+WS VST +D+  F D T FD +RFEGAGPTP+++VPFGGGPRMCLGKEFAR+E+L 
Sbjct: 376  WKLHWSAVSTQRDEANFEDVTRFDPSRFEGAGPTPFTFVPFGGGPRMCLGKEFARLEVLA 435

Query: 314  FLHNVVKRFKWDLLIPNEKMEYDPMPIPVNGLPISLHPH 198
            FLHN+V  FKWDLLIP+EK+EYDPM  P  GLPI LHPH
Sbjct: 436  FLHNIVTNFKWDLLIPDEKIEYDPMATPAKGLPIRLHPH 474


>ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 716B2-like [Vitis
            vinifera]
          Length = 473

 Score =  468 bits (1203), Expect = e-129
 Identities = 220/339 (64%), Positives = 271/339 (79%)
 Frame = -2

Query: 1214 LSPDAFSRLYINIMDLVTQKHISTHWQGKQEVKVYNTIKLYTFELACRLFTSLEDPDHIK 1035
            L+PDA  R Y+  MDLVTQ HI T W+G +E++VY  IKLYTFELACRLF S++DP HI 
Sbjct: 136  LNPDALKR-YVETMDLVTQHHIRTQWEGNEEMQVYPIIKLYTFELACRLFASMDDPGHIS 194

Query: 1034 KLASQFNIFLKGIIQIPLNVPGTRFYYAMRAANAIRKDLLEITRQRRIDLEQKTASPSQD 855
            KLA+ FN+FLKGII  P+N PGT+F YA +AANAIR++L  +  +RR+ LE+KTAS +QD
Sbjct: 195  KLAAHFNVFLKGIIDFPINFPGTKFXYATKAANAIREELRVMPMERRVALEEKTASATQD 254

Query: 854  LLSHLLVSCDEHGRSLTEDEIVNNILTLLFAGHDTSSVSITLAMKILAEKPQVYQKVLDE 675
            LLSHLLV+ D  GR LTE EI++NIL LLFAGHDT++ +ITL +K L E P+VY KVL E
Sbjct: 255  LLSHLLVTADASGRFLTEMEIIDNILLLLFAGHDTTASAITLLIKYLGELPEVYAKVLRE 314

Query: 674  HMDIAASKRKDELLQWDDIQKMKYTWNVVCEVMRLTPPVTGAFREALVEFDFAGYTIPKG 495
             +DI  SK   ELLQW D QKM+Y+W+V  EVMRL+PP +G+FREALV+F +AGY IPKG
Sbjct: 315  KIDITESKEPGELLQWKDXQKMRYSWHVASEVMRLSPPASGSFREALVDFSYAGYNIPKG 374

Query: 494  WKLYWSTVSTNKDDKYFPDPTEFDATRFEGAGPTPYSYVPFGGGPRMCLGKEFARMEILV 315
            WKLYW T ST +D  +F +P  FDA+RFEGAGP P+SYVPFGGGPRMCLG+EFAR++ILV
Sbjct: 375  WKLYWGTGSTQRDPAFFRNPDNFDASRFEGAGPAPFSYVPFGGGPRMCLGQEFARLQILV 434

Query: 314  FLHNVVKRFKWDLLIPNEKMEYDPMPIPVNGLPISLHPH 198
            F+HN+VKRF WDLL P+EK+E+ PM  P+ GLPI LHPH
Sbjct: 435  FMHNIVKRFTWDLLNPDEKIEHSPMLAPLEGLPIRLHPH 473


>gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P450 [Artemisia
            annua]
          Length = 480

 Score =  467 bits (1201), Expect = e-129
 Identities = 218/340 (64%), Positives = 271/340 (79%), Gaps = 1/340 (0%)
 Frame = -2

Query: 1214 LSPDAFSRLYINIMDLVTQKHISTHWQGKQEVKVYNTIKLYTFELACRLFTSLEDPDHIK 1035
            L PDA S  Y   M++VT+ HI  HWQGK E+KV+ T++ Y FELACRLF SL+DP H+ 
Sbjct: 139  LGPDALSNRYTGTMEVVTRLHIQNHWQGKSELKVFETVRPYLFELACRLFLSLDDPKHVA 198

Query: 1034 KLASQFNIFLKGIIQIPLNVPGTRFYYAMRAANAIRKDLLEITRQRRIDLEQKTASPS-Q 858
            +L + FN FLKG+ ++P+N+PGTRFY A RAANAI+K L+ I +QRR  L+Q+  S S +
Sbjct: 199  ELGTLFNTFLKGLTELPINIPGTRFYRAKRAANAIKKQLIVIIKQRRQALKQEDQSSSFE 258

Query: 857  DLLSHLLVSCDEHGRSLTEDEIVNNILTLLFAGHDTSSVSITLAMKILAEKPQVYQKVLD 678
            DLLSHLLVS DE+GR L+E EI NN+L LLFAGHDTS+VSITL MK LAE PQVY  VL 
Sbjct: 259  DLLSHLLVSSDENGRFLSEAEIANNVLLLLFAGHDTSAVSITLLMKSLAEHPQVYDNVLK 318

Query: 677  EHMDIAASKRKDELLQWDDIQKMKYTWNVVCEVMRLTPPVTGAFREALVEFDFAGYTIPK 498
            E + I  +K   E+L W+DIQKM+Y+W VVCEVMRL PPV G+FREALV+F++AGYTIPK
Sbjct: 319  EQLGILEAKAPGEMLNWEDIQKMRYSWYVVCEVMRLIPPVVGSFREALVDFEYAGYTIPK 378

Query: 497  GWKLYWSTVSTNKDDKYFPDPTEFDATRFEGAGPTPYSYVPFGGGPRMCLGKEFARMEIL 318
            GWK+ WS V T+K++  FP+ T+FD +RFEGAGPTP++YVPFGGGPRMCLGKE AR+ IL
Sbjct: 379  GWKIIWSAVMTHKEENNFPNATKFDPSRFEGAGPTPFTYVPFGGGPRMCLGKELARVRIL 438

Query: 317  VFLHNVVKRFKWDLLIPNEKMEYDPMPIPVNGLPISLHPH 198
            VFLHN++ +FKWDLLIP+EK+ YDP+  PV GLP+ LHPH
Sbjct: 439  VFLHNIMTKFKWDLLIPDEKIGYDPLATPVKGLPVRLHPH 478


>ref|XP_003524231.1| PREDICTED: cytochrome P450 716B2-like [Glycine max]
          Length = 486

 Score =  445 bits (1144), Expect = e-122
 Identities = 206/333 (61%), Positives = 267/333 (80%)
 Frame = -2

Query: 1193 RLYINIMDLVTQKHISTHWQGKQEVKVYNTIKLYTFELACRLFTSLEDPDHIKKLASQFN 1014
            R Y+  MD + Q+HI T+W+GK++V VY  ++LYTFELAC LF S+ED DHI KL+ +F+
Sbjct: 153  RNYLPKMDSIAQRHIDTYWEGKEQVCVYPIVQLYTFELACCLFLSIEDSDHISKLSLKFD 212

Query: 1013 IFLKGIIQIPLNVPGTRFYYAMRAANAIRKDLLEITRQRRIDLEQKTASPSQDLLSHLLV 834
             FLKGII  PLNVPGTRFY AM+AA+ IRK++  I ++R++DLE+K  SP+QDLLSH+LV
Sbjct: 213  EFLKGIIGFPLNVPGTRFYRAMKAADVIRKEIKMILKKRKVDLEEKRVSPTQDLLSHMLV 272

Query: 833  SCDEHGRSLTEDEIVNNILTLLFAGHDTSSVSITLAMKILAEKPQVYQKVLDEHMDIAAS 654
            + D  GR +TE EI++NIL LLFAGHDTS   ++L MK L + PQVY+ VL+E ++I+  
Sbjct: 273  TSDPSGRFMTEMEILDNILLLLFAGHDTSRSVLSLVMKYLGQLPQVYEHVLEEQLEISQG 332

Query: 653  KRKDELLQWDDIQKMKYTWNVVCEVMRLTPPVTGAFREALVEFDFAGYTIPKGWKLYWST 474
            K   +LLQW+D+QKMKY+WNV  EVMRL+PPV+GA+REA+ +F +A Y IPKGWKL+W+T
Sbjct: 333  KEAGQLLQWEDVQKMKYSWNVASEVMRLSPPVSGAYREAIKDFTYADYNIPKGWKLHWNT 392

Query: 473  VSTNKDDKYFPDPTEFDATRFEGAGPTPYSYVPFGGGPRMCLGKEFARMEILVFLHNVVK 294
             S++KD   F +P  FDA+RFEGAGPTP+SYVPFGGGPRMCLG EFAR+EILVF+HN+VK
Sbjct: 393  GSSHKDPTLFSNPETFDASRFEGAGPTPFSYVPFGGGPRMCLGLEFARLEILVFMHNIVK 452

Query: 293  RFKWDLLIPNEKMEYDPMPIPVNGLPISLHPHY 195
            RFKWDL+IP+E  +YDPM  P+ GL I LHP +
Sbjct: 453  RFKWDLVIPDEMFKYDPMLEPIKGLAIRLHPSH 485