BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00010326
         (1014 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI17099.3| unnamed protein product [Vitis vinifera]              194   1e-98
ref|XP_002271001.1| PREDICTED: putative phospholipid:diacylglyce...   194   3e-98
ref|XP_004145827.1| PREDICTED: putative phospholipid:diacylglyce...   187   6e-94
ref|XP_002328081.1| predicted protein [Populus trichocarpa] gi|2...   189   8e-94
gb|AAS79590.1| putative phosphatidylcholine-sterol acyltransfera...   199   2e-93

>emb|CBI17099.3| unnamed protein product [Vitis vinifera]
          Length = 1026

 Score =  194 bits (493), Expect(3) = 1e-98
 Identities = 88/133 (66%), Positives = 107/133 (80%)
 Frame = +2

Query: 614  GYEDRNMYMAAYDWRLSFQNTEVRDKSLSRLKGQIEIMYTTNGDKKVVVLPHSMGAVYFL 793
            GYE +N+YMAAYDWRLSFQNTE+RD++LSRLK +IE+M+ TNG+KKVVV+PHSMG +YF 
Sbjct: 187  GYEGKNLYMAAYDWRLSFQNTEIRDQALSRLKSKIELMFVTNGNKKVVVVPHSMGVIYFF 246

Query: 794  HFLKWVEXXXXXXXXXXXXWCAKHIKAVMNIGPTFLGVPKAFSTILSAEGRDISLIRAMA 973
            HFLKWVE            WCAKHIKA+MNIGP FLGVPKA S I SAE +D++ +R+M 
Sbjct: 247  HFLKWVESPPPMGGGGGTGWCAKHIKAIMNIGPAFLGVPKAVSNIFSAEAKDVAFVRSMD 306

Query: 974  SGLLESEFLGLQT 1012
             G+L+SE LGLQT
Sbjct: 307  PGVLDSEILGLQT 319



 Score =  118 bits (295), Expect(3) = 1e-98
 Identities = 61/121 (50%), Positives = 75/121 (61%)
 Frame = +1

Query: 61  SELGFHKSAIVEAKSINLDEKVFDKNKKRGKNKRQPVGWKCMDNCCWMIGYICXXXXXXX 240
           S L F K   VE    +    V +K KK+  +KRQP  W+CMD CC  +GY+C       
Sbjct: 3   SILRFRKLCYVEPVKCSSLGTVGEKQKKKS-SKRQPREWRCMDYCCLTVGYVCTAWWLLL 61

Query: 241 XXXXXIPVSLPGVKGPEILGARLKREGLVANHLVVLVSGIITGGLELWEDKPCAEPLFRK 420
                +P +LPG + PE  GARL+REGL A H VVLV GI+TGGLELWE +PC+E LFRK
Sbjct: 62  FLYHCLPAALPGFQVPEAPGARLRREGLTALHPVVLVPGIVTGGLELWEGRPCSEGLFRK 121

Query: 421 R 423
           R
Sbjct: 122 R 122



 Score = 96.7 bits (239), Expect(3) = 1e-98
 Identities = 42/60 (70%), Positives = 49/60 (81%)
 Frame = +3

Query: 435 WGSGFTDIFKRPMCWMEHLSLHYETGLHPQGI*IRPVPGLAVADYFAPGYFV*AILI*NL 614
           WG  FT+IFKRP+CW+EHL+LH +TGL P GI +R VPGL  ADYFAPGYFV A+LI NL
Sbjct: 124 WGGSFTEIFKRPLCWLEHLTLHNQTGLDPPGIRVRAVPGLVAADYFAPGYFVWAVLIENL 183


>ref|XP_002271001.1| PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2
            [Vitis vinifera]
          Length = 688

 Score =  194 bits (493), Expect(3) = 3e-98
 Identities = 88/133 (66%), Positives = 107/133 (80%)
 Frame = +2

Query: 614  GYEDRNMYMAAYDWRLSFQNTEVRDKSLSRLKGQIEIMYTTNGDKKVVVLPHSMGAVYFL 793
            GYE +N+YMAAYDWRLSFQNTE+RD++LSRLK +IE+M+ TNG+KKVVV+PHSMG +YF 
Sbjct: 210  GYEGKNLYMAAYDWRLSFQNTEIRDQALSRLKSKIELMFVTNGNKKVVVVPHSMGVIYFF 269

Query: 794  HFLKWVEXXXXXXXXXXXXWCAKHIKAVMNIGPTFLGVPKAFSTILSAEGRDISLIRAMA 973
            HFLKWVE            WCAKHIKA+MNIGP FLGVPKA S I SAE +D++ +R+M 
Sbjct: 270  HFLKWVESPPPMGGGGGTGWCAKHIKAIMNIGPAFLGVPKAVSNIFSAEAKDVAFVRSMD 329

Query: 974  SGLLESEFLGLQT 1012
             G+L+SE LGLQT
Sbjct: 330  PGVLDSEILGLQT 342



 Score =  116 bits (291), Expect(3) = 3e-98
 Identities = 59/127 (46%), Positives = 78/127 (61%), Gaps = 6/127 (4%)
 Frame = +1

Query: 61  SELGFH--KSAIVEAK----SINLDEKVFDKNKKRGKNKRQPVGWKCMDNCCWMIGYICX 222
           S LGF   ++  +E K    ++   E    + +K+  +KRQP  W+CMD CC  +GY+C 
Sbjct: 19  SSLGFESFETPKIEKKDDRGAVKAVESTVGEKQKKKSSKRQPREWRCMDYCCLTVGYVCT 78

Query: 223 XXXXXXXXXXXIPVSLPGVKGPEILGARLKREGLVANHLVVLVSGIITGGLELWEDKPCA 402
                      +P +LPG + PE  GARL+REGL A H VVLV GI+TGGLELWE +PC+
Sbjct: 79  AWWLLLFLYHCLPAALPGFQVPEAPGARLRREGLTALHPVVLVPGIVTGGLELWEGRPCS 138

Query: 403 EPLFRKR 423
           E LFRKR
Sbjct: 139 EGLFRKR 145



 Score = 96.7 bits (239), Expect(3) = 3e-98
 Identities = 42/60 (70%), Positives = 49/60 (81%)
 Frame = +3

Query: 435 WGSGFTDIFKRPMCWMEHLSLHYETGLHPQGI*IRPVPGLAVADYFAPGYFV*AILI*NL 614
           WG  FT+IFKRP+CW+EHL+LH +TGL P GI +R VPGL  ADYFAPGYFV A+LI NL
Sbjct: 147 WGGSFTEIFKRPLCWLEHLTLHNQTGLDPPGIRVRAVPGLVAADYFAPGYFVWAVLIENL 206


>ref|XP_004145827.1| PREDICTED: putative phospholipid:diacylglycerol acyltransferase
            2-like [Cucumis sativus] gi|449496312|ref|XP_004160101.1|
            PREDICTED: putative phospholipid:diacylglycerol
            acyltransferase 2-like [Cucumis sativus]
          Length = 690

 Score =  187 bits (474), Expect(3) = 6e-94
 Identities = 86/135 (63%), Positives = 105/135 (77%)
 Frame = +2

Query: 608  EFGYEDRNMYMAAYDWRLSFQNTEVRDKSLSRLKGQIEIMYTTNGDKKVVVLPHSMGAVY 787
            + GY+ +N++MAAYDWR++FQNTEVRD++LSRLK +IE+MY TNG  KVVV+PHSMG +Y
Sbjct: 215  KIGYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYATNGGNKVVVVPHSMGVLY 274

Query: 788  FLHFLKWVEXXXXXXXXXXXXWCAKHIKAVMNIGPTFLGVPKAFSTILSAEGRDISLIRA 967
            FLHF+KWVE            WCAKHIKA+MNIG TFLG PKA S ILSAE R ++L+RA
Sbjct: 275  FLHFMKWVEAPPPMGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRA 334

Query: 968  MASGLLESEFLGLQT 1012
            M  G L+SE LG QT
Sbjct: 335  MTPGFLKSEILGFQT 349



 Score =  118 bits (295), Expect(3) = 6e-94
 Identities = 62/138 (44%), Positives = 84/138 (60%)
 Frame = +1

Query: 10  LGLKSTQIEVHG*SSSHSELGFHKSAIVEAKSINLDEKVFDKNKKRGKNKRQPVGWKCMD 189
           +GL+S  IE H   +  + +  +   +++ K    D+K  +KN  RG+++     WKC+D
Sbjct: 21  MGLQS--IEAHKSDNKRNVIVAYVKNLMQKKQDKDDKKKRNKNINRGQSRE----WKCLD 74

Query: 190 NCCWMIGYICXXXXXXXXXXXXIPVSLPGVKGPEILGARLKREGLVANHLVVLVSGIITG 369
           NCCW+IGYIC            +P+SLPG    +  GA LKREG+   H VVLV GI+TG
Sbjct: 75  NCCWIIGYICTFWWLMFFLYHYLPLSLPGFPVIDSPGAVLKREGISGRHPVVLVPGIVTG 134

Query: 370 GLELWEDKPCAEPLFRKR 423
           GLELW+ KPCAE LFRKR
Sbjct: 135 GLELWDGKPCAEGLFRKR 152



 Score = 88.2 bits (217), Expect(3) = 6e-94
 Identities = 40/60 (66%), Positives = 45/60 (75%)
 Frame = +3

Query: 435 WGSGFTDIFKRPMCWMEHLSLHYETGLHPQGI*IRPVPGLAVADYFAPGYFV*AILI*NL 614
           WG  FT+  KRP+CW+EHLSL  ETGL P GI +RPV GL  ADYFA GYFV A+LI NL
Sbjct: 154 WGGSFTETLKRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENL 213


>ref|XP_002328081.1| predicted protein [Populus trichocarpa] gi|222837596|gb|EEE75961.1|
            predicted protein [Populus trichocarpa]
          Length = 680

 Score =  189 bits (481), Expect(3) = 8e-94
 Identities = 89/134 (66%), Positives = 105/134 (78%)
 Frame = +2

Query: 608  EFGYEDRNMYMAAYDWRLSFQNTEVRDKSLSRLKGQIEIMYTTNGDKKVVVLPHSMGAVY 787
            + GYE +NM+MAAYDWRLSFQNTE+RD++LSRLK QIE+MY TNG  KVVV+PHSMG +Y
Sbjct: 200  KIGYEGKNMHMAAYDWRLSFQNTEIRDQTLSRLKSQIELMYVTNGYMKVVVVPHSMGVIY 259

Query: 788  FLHFLKWVEXXXXXXXXXXXXWCAKHIKAVMNIGPTFLGVPKAFSTILSAEGRDISLIRA 967
            FLHFLKWVE            WCAKHIKA+MNIGP FLGVPKA S + SAE +D++ IRA
Sbjct: 260  FLHFLKWVETPPPMGGGGGPGWCAKHIKAIMNIGPVFLGVPKAVSNLFSAEAKDVASIRA 319

Query: 968  MASGLLESEFLGLQ 1009
            M  G+L+SE L LQ
Sbjct: 320  MDPGVLDSEILRLQ 333



 Score =  113 bits (283), Expect(3) = 8e-94
 Identities = 55/98 (56%), Positives = 65/98 (66%)
 Frame = +1

Query: 130 DKNKKRGKNKRQPVGWKCMDNCCWMIGYICXXXXXXXXXXXXIPVSLPGVKGPEILGARL 309
           +KN+KR  NKRQP  W C+++CCW IGY+C            +P + PG +  E  G RL
Sbjct: 42  EKNEKR--NKRQPKEWSCINSCCWAIGYLCTTWWLLLVLFNCMPATFPGFQVLESPGTRL 99

Query: 310 KREGLVANHLVVLVSGIITGGLELWEDKPCAEPLFRKR 423
           K EGL A H VVLV GI+TGGLELWE KPCAE LFRKR
Sbjct: 100 KLEGLTALHPVVLVPGIVTGGLELWEGKPCAEGLFRKR 137



 Score = 89.7 bits (221), Expect(3) = 8e-94
 Identities = 40/60 (66%), Positives = 47/60 (78%)
 Frame = +3

Query: 435 WGSGFTDIFKRPMCWMEHLSLHYETGLHPQGI*IRPVPGLAVADYFAPGYFV*AILI*NL 614
           WG  FT++ KRP+C +EHL+LH ETGL P GI +R VPGL  ADYFAPGYFV A+LI NL
Sbjct: 139 WGGSFTEVLKRPLCLLEHLALHNETGLDPPGIRLRAVPGLVAADYFAPGYFVWAVLIENL 198


>gb|AAS79590.1| putative phosphatidylcholine-sterol acyltransferase [Ipomoea trifida]
            gi|117166020|dbj|BAF36322.1| hypothetical protein
            [Ipomoea trifida]
          Length = 667

 Score =  199 bits (506), Expect(3) = 2e-93
 Identities = 93/133 (69%), Positives = 108/133 (81%)
 Frame = +2

Query: 614  GYEDRNMYMAAYDWRLSFQNTEVRDKSLSRLKGQIEIMYTTNGDKKVVVLPHSMGAVYFL 793
            GYE +NMYMAAYDWRLSFQNTEVRD++LSRLK +IE+MY TNG+KKVVV+PHSMG +YFL
Sbjct: 189  GYEQKNMYMAAYDWRLSFQNTEVRDQALSRLKSKIELMYATNGNKKVVVVPHSMGVLYFL 248

Query: 794  HFLKWVEXXXXXXXXXXXXWCAKHIKAVMNIGPTFLGVPKAFSTILSAEGRDISLIRAMA 973
            HFLKWVE            WCAKHIKA+MN+GP FLGVPK+FS+ILSAEG+DIS IR+MA
Sbjct: 249  HFLKWVESPPPMGGGGGPSWCAKHIKAIMNVGPAFLGVPKSFSSILSAEGKDISFIRSMA 308

Query: 974  SGLLESEFLGLQT 1012
             GL +  F   QT
Sbjct: 309  PGLFDETFRRFQT 321



 Score = 99.8 bits (247), Expect(3) = 2e-93
 Identities = 61/127 (48%), Positives = 75/127 (59%), Gaps = 6/127 (4%)
 Frame = +1

Query: 61  SELGFHKSAIVEAKSI-----NLDEKVFDKNKKRGKNKRQPVGWKCMDNCCWMIGYICXX 225
           S L F K   VEA        N DEK  D  +K GK +++  G +C+D+CC +IGY+C  
Sbjct: 3   SVLRFRKLCYVEAVKCSSGKRNGDEKKRDNVEKVGKKQQRKEG-RCIDSCCRVIGYLCTT 61

Query: 226 XXXXXXXXXXIPVSLPGVKGP-EILGARLKREGLVANHLVVLVSGIITGGLELWEDKPCA 402
                         LPG+K P +  GARLKREGL   H VVLV GI+TGGLELWE +PCA
Sbjct: 62  WWLLLFLGNF----LPGLKAPADSPGARLKREGLTGFHPVVLVPGIVTGGLELWEGRPCA 117

Query: 403 EPLFRKR 423
           + LFRKR
Sbjct: 118 QGLFRKR 124



 Score = 92.4 bits (228), Expect(3) = 2e-93
 Identities = 41/60 (68%), Positives = 47/60 (78%)
 Frame = +3

Query: 435 WGSGFTDIFKRPMCWMEHLSLHYETGLHPQGI*IRPVPGLAVADYFAPGYFV*AILI*NL 614
           WG  F ++FKRP+CW+EHLSL  ETGL P GI +R VPGL  ADYFAPGYFV A+LI NL
Sbjct: 126 WGGSFVEMFKRPLCWLEHLSLDNETGLDPPGIRVRAVPGLVAADYFAPGYFVWAVLIENL 185