BLASTX nr result
ID: Bupleurum21_contig00010325
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00010325
(1654 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002329318.1| predicted protein [Populus trichocarpa] gi|2... 531 e-148
ref|XP_002283177.1| PREDICTED: translation initiation factor eIF... 520 e-145
ref|XP_002511987.1| translation initiation factor 2b, delta subu... 514 e-143
ref|XP_002521202.1| translation initiation factor 2b, delta subu... 513 e-143
emb|CBI16861.3| unnamed protein product [Vitis vinifera] 499 e-138
>ref|XP_002329318.1| predicted protein [Populus trichocarpa] gi|222870772|gb|EEF07903.1|
predicted protein [Populus trichocarpa]
Length = 634
Score = 531 bits (1368), Expect = e-148
Identities = 306/539 (56%), Positives = 358/539 (66%), Gaps = 55/539 (10%)
Frame = +3
Query: 201 MDLRRTSRVI-DPKVRKVGFFAPKPEQTQSGPSDSP-----------------SIAASLL 326
MD RR R + DPKVRKVGFF P+++ GP D P S SL
Sbjct: 1 MDPRRAPRTVSDPKVRKVGFFTDPPDRSLWGPPDHPISSFPTSPPSIVDSSPPSAGNSLS 60
Query: 327 PVIIPPARQAS--------------------------DNPTRAIKVLNEASDSR----GQ 416
PV+IPP R +S D+ A+ A+ S G
Sbjct: 61 PVMIPPPRHSSATLSPLRRDSAEFFPPPVSPTASSLSDDVASAMMRRGVAAASSSLPGGL 120
Query: 417 EVDCVAV---GSFVGDKIQHTPQVVSN--DEKGGELPARMS--LKSDSKPLKEKTTXXXX 575
D AV S V T VV+N EKGG + M S SK LKEKTT
Sbjct: 121 GFDIAAVKSAASSVPASGLTTVSVVNNLPIEKGGGVAVEMQNDQSSRSKSLKEKTTKAER 180
Query: 576 XXXXXXXXXXXXXXXXXXXXXKTPGAATGGEASVQTGKVVKQPRQKKDGPSMASIEKTVG 755
KTP AA+G AS ++ K VK P QK D ++A EK G
Sbjct: 181 RALQEAQRAAKAAAKAEGG--KTPAAASGAAASAKSAKSVKPPSQKNDSATVAVSEKKGG 238
Query: 756 DRPQEKDKKKDVPPPRMQFDDIGRVEKAKKRSVVKQVEATNRVEYFLHLPQYERGTQLPD 935
DRP EKD+KK+VP PRMQ+DD RVEKAK+R+VVKQ EA NRVE F HLPQYER TQL +
Sbjct: 239 DRPPEKDRKKEVPQPRMQYDDKSRVEKAKRRAVVKQTEARNRVELFRHLPQYERRTQLTE 298
Query: 936 LETKFFQLDYIHPAVYKVGLQYLAGDITGGNARCIAMLQAFHKAVEDYSAPSEKTVSRDL 1115
LE+KFF+LD +HPAVYKVGLQYL+GD+ GGNARCIAMLQAF +A++DYS PSEKT++RDL
Sbjct: 299 LESKFFELDPMHPAVYKVGLQYLSGDVCGGNARCIAMLQAFQEAIKDYSTPSEKTLARDL 358
Query: 1116 LAKINSYVSYLIECRPLSVSMGNAIRFLKDHITKLPLDISESEAKASICSDINRFITEKI 1295
AKI SY+S+LIECRPLS+SMGNAIRFLK+ I KLPL +SE+E KA++ SDI+RFI EKI
Sbjct: 359 TAKIGSYLSFLIECRPLSISMGNAIRFLKNRIAKLPLTLSEAEVKATLHSDIDRFINEKI 418
Query: 1296 VLADRIIVKHAVTKIRDGDVLLTYGSSTVVEMILLQAHVLGTRFRVVVVDSRPKLEGQAL 1475
+LAD++IVKHAVTKI DGDVLLTYGS + VEM+LL AH LG +FRVVVVDSRPKLEGQ L
Sbjct: 419 ILADKVIVKHAVTKISDGDVLLTYGSPSAVEMVLLHAHELGKQFRVVVVDSRPKLEGQLL 478
Query: 1476 VRRLVQNGVSCTYTHINAISYIMHEITRVFLGAASVLSNGTVYSRVGTACVAMVAHEFR 1652
+ RLV G+ CTYTH+NAISYIMHE+TRVFLGA+SVLSNGTVYSRVGTACVAMVAH FR
Sbjct: 479 LHRLVGKGIDCTYTHVNAISYIMHEVTRVFLGASSVLSNGTVYSRVGTACVAMVAHAFR 537
>ref|XP_002283177.1| PREDICTED: translation initiation factor eIF-2B subunit delta-like
[Vitis vinifera]
Length = 650
Score = 520 bits (1338), Expect = e-145
Identities = 306/555 (55%), Positives = 362/555 (65%), Gaps = 71/555 (12%)
Frame = +3
Query: 201 MDLRRTSRV-IDPKVRKVGFFAP--KPEQTQSGP--SDSPSIAA------SLLPVII--- 338
MD RR +RV IDPKVR+VGFFAP P +TQSGP S SP +A SL PV+I
Sbjct: 1 MDPRRGARVVIDPKVRQVGFFAPGASPGRTQSGPPESSSPPVADNSPTGNSLSPVMIPPP 60
Query: 339 ---------------PPARQASD----------------NPTRAI-------KVLNEASD 404
PP R+ D +PT + + +AS
Sbjct: 61 RHASDNLLSARPALYPPLRRPGDTIPVGSYNPSEFFHGTSPTASFYSKVGDGEFSEDASG 120
Query: 405 SR-----------------GQEVDCVAVGSFVGDKIQHTPQVVSNDEKGGELPARMSLKS 533
+ G ++ V + + + P +S G + + +
Sbjct: 121 AGWTHRGNSGKVASSFPGGGFDLTAVKPSNLTTVSVVNMPPGISVKSGGASIEVQNEQPA 180
Query: 534 DSKPLKEKTTXXXXXXXXXXXXXXXXXXXXXXXXXKTPGAATGGEASVQT--GKVVKQPR 707
S+ LKEKT+ K P AA+G SV KV K
Sbjct: 181 SSRSLKEKTSKAERRALQEAQRAAKAAAKAEVS--KAPIAASGVATSVNAKPAKVSKLSS 238
Query: 708 QKKDGPSMASIEKTVGDRPQEKDKKKDVPPPRMQFDDIGRVEKAKKRSVVKQVEATNRVE 887
QKKD S+ + EK DRP EKD+KKDVP PRMQFDD RVEKAK+R+VV+ +E NRVE
Sbjct: 239 QKKDSASVTASEKKGVDRPPEKDRKKDVPHPRMQFDDKSRVEKAKRRAVVRPIEVRNRVE 298
Query: 888 YFLHLPQYERGTQLPDLETKFFQLDYIHPAVYKVGLQYLAGDITGGNARCIAMLQAFHKA 1067
F HLPQYERGT LPDLE+KFF LD +HPAVYKVGLQYLAGD++GGNARCIAMLQAF +A
Sbjct: 299 LFRHLPQYERGTLLPDLESKFFLLDPMHPAVYKVGLQYLAGDVSGGNARCIAMLQAFQEA 358
Query: 1068 VEDYSAPSEKTVSRDLLAKINSYVSYLIECRPLSVSMGNAIRFLKDHITKLPLDISESEA 1247
++DYS P EKT+ RDL A+++SYVS+LIECRPLS+SMGNAIRFLK I KLPL +SESEA
Sbjct: 359 IKDYSTPPEKTLVRDLTARLSSYVSFLIECRPLSISMGNAIRFLKSRIAKLPLSLSESEA 418
Query: 1248 KASICSDINRFITEKIVLADRIIVKHAVTKIRDGDVLLTYGSSTVVEMILLQAHVLGTRF 1427
KAS+ SDINRFI EKIVLAD++IV+HAVTKIRDGDVLLTYGSS+ VEMILL AH LG +F
Sbjct: 419 KASLQSDINRFINEKIVLADKVIVRHAVTKIRDGDVLLTYGSSSAVEMILLYAHELGKQF 478
Query: 1428 RVVVVDSRPKLEGQALVRRLVQNGVSCTYTHINAISYIMHEITRVFLGAASVLSNGTVYS 1607
RVVVVDSRPKLEGQ L+RRLV G+SCTYTHINA+SYIMHE+TRVFLGA+SVLSNGTVYS
Sbjct: 479 RVVVVDSRPKLEGQLLLRRLVGKGLSCTYTHINAVSYIMHEVTRVFLGASSVLSNGTVYS 538
Query: 1608 RVGTACVAMVAHEFR 1652
RVGTACV+MVAH FR
Sbjct: 539 RVGTACVSMVAHAFR 553
>ref|XP_002511987.1| translation initiation factor 2b, delta subunit, putative [Ricinus
communis] gi|223549167|gb|EEF50656.1| translation
initiation factor 2b, delta subunit, putative [Ricinus
communis]
Length = 664
Score = 514 bits (1324), Expect = e-143
Identities = 306/574 (53%), Positives = 361/574 (62%), Gaps = 91/574 (15%)
Frame = +3
Query: 201 MDLRRTSRVIDPKVRKVGFFAPK----PEQTQSGPSDSPS-------IAASLLPVIIPPA 347
MD RR R + PKVR+VGFF P P +TQSGP D+ S + SL PV+IPP
Sbjct: 1 MDNRRGYRAV-PKVRQVGFFTPNEPPAPSRTQSGPPDATSPPLSNSPASNSLSPVMIPPP 59
Query: 348 RQASD----------------------------------NPTRAIKVLNEASDSRGQEVD 425
R SD NPT ++ V++ S VD
Sbjct: 60 RHLSDSLAHRATSPLPVPESSAFRRPMTGGEHVPVIGSYNPTESLLVVSPPLTSPSSRVD 119
Query: 426 C-------------------------VAVGSFVGDKIQHTP----QVVSNDEK------- 497
A SF G T +VV +K
Sbjct: 120 GGGDGDGELFSEESSPGWFRRSNSAKFAASSFPGGGFDLTSIKSSEVVDAKKKPVVEKSG 179
Query: 498 ---GGELPARMSLKSDSKPLKEKTTXXXXXXXXXXXXXXXXXXXXXXXXXKTPGAATGGE 668
G L ++L S SKPLK KTT + G ATG
Sbjct: 180 GADGTVLQNELALSS-SKPLKAKTTKAERRALQESQRAAKAA---------SKGEATGKP 229
Query: 669 ASVQTGK---VVKQPRQKKDGP----SMASIEKTVGDRPQEKDKKKDVPPPRMQFDDIGR 827
+++ G +QP QKKD P S+A+ E+ DRP EK++KKDVP PRMQFDD R
Sbjct: 230 STISEGASKPAKQQPPQKKDAPPSAPSIAASERKGVDRPSEKERKKDVPQPRMQFDDKNR 289
Query: 828 VEKAKKRSVVKQVEATNRVEYFLHLPQYERGTQLPDLETKFFQLDYIHPAVYKVGLQYLA 1007
VEKAK+R+VV Q EA NRVE F HLPQYE GTQLPDLE+KFFQLD +HPAV KVGLQYLA
Sbjct: 290 VEKAKRRAVVNQTEARNRVELFRHLPQYEHGTQLPDLESKFFQLDLMHPAVCKVGLQYLA 349
Query: 1008 GDITGGNARCIAMLQAFHKAVEDYSAPSEKTVSRDLLAKINSYVSYLIECRPLSVSMGNA 1187
GDI+GGNARCIAML AF +A++DYS P EK+++RDL AKINSYVS+LIECRPLS+SMGNA
Sbjct: 350 GDISGGNARCIAMLLAFQEAIKDYSTPPEKSLTRDLTAKINSYVSFLIECRPLSMSMGNA 409
Query: 1188 IRFLKDHITKLPLDISESEAKASICSDINRFITEKIVLADRIIVKHAVTKIRDGDVLLTY 1367
IRFLK I P+ +ESEAKAS+CSDI+RFI EKI+LAD++IV+HA +K+RDGDVLLTY
Sbjct: 410 IRFLKSRIANFPITFAESEAKASLCSDIDRFINEKILLADKVIVRHAASKVRDGDVLLTY 469
Query: 1368 GSSTVVEMILLQAHVLGTRFRVVVVDSRPKLEGQALVRRLVQNGVSCTYTHINAISYIMH 1547
GSS VVEMILL AH LG +FRVV+VD+RPKLEGQAL+RRLV G+SCTYTHINA+SYIMH
Sbjct: 470 GSSCVVEMILLYAHELGKQFRVVIVDARPKLEGQALLRRLVAKGLSCTYTHINAVSYIMH 529
Query: 1548 EITRVFLGAASVLSNGTVYSRVGTACVAMVAHEF 1649
E+TRVFLGA+SVLSNGTVYSRVGTACVAMVAH F
Sbjct: 530 EVTRVFLGASSVLSNGTVYSRVGTACVAMVAHAF 563
>ref|XP_002521202.1| translation initiation factor 2b, delta subunit, putative [Ricinus
communis] gi|223539567|gb|EEF41154.1| translation
initiation factor 2b, delta subunit, putative [Ricinus
communis]
Length = 638
Score = 513 bits (1320), Expect = e-143
Identities = 304/549 (55%), Positives = 354/549 (64%), Gaps = 66/549 (12%)
Frame = +3
Query: 201 MDLRRTSRVI-DPKVRKVGFFAP---KPEQTQSGPSD--------------SPSIAASLL 326
MD RR SR + DPKVR+VGFF P P+++QSGP D SPS SL
Sbjct: 1 MDPRRASRTVSDPKVRQVGFFTPGASSPDRSQSGPPDPHPISSSPPTITDLSPSSGNSLS 60
Query: 327 PVIIPPARQAS------------------DNPTRAIKVLNEA---------SDS-RGQE- 419
PV+IPP R S N A ++ A SDS RG
Sbjct: 61 PVMIPPPRHVSAPLSPLLRDSAPPGTMVNSNSNNATELFTAATSPTATTSFSDSGRGGTA 120
Query: 420 -------------VDCVAVGSFVGDKIQHTPQVVSN-----DEKGG-ELPARMSLKSDSK 542
+D S V T V N DEK G + S +S+ K
Sbjct: 121 PSSSYPGGGGVGGLDMPQTSSSVPGSGLTTVSVFVNLPPGLDEKAGISTDVQNSNRSNLK 180
Query: 543 PLKEKTTXXXXXXXXXXXXXXXXXXXXXXXXXKTPGAATGGEASVQTGKVVKQPRQKKDG 722
LKEKTT K GAA A+ + K K P QK D
Sbjct: 181 RLKEKTTKAERRALQEAQQAAKAAA-------KAEGAAI--PANAKLAKSAKVPSQKNDN 231
Query: 723 PSMASIEKTVGDRPQEKDKKKDVPPPRMQFDDIGRVEKAKKRSVVKQVEATNRVEYFLHL 902
A+ EK G+ P EKD+KKDVP PRMQ+DD RVEKAKKR+VVKQ EA NRVE F HL
Sbjct: 232 APTAASEKKGGEHPPEKDRKKDVPQPRMQYDDKSRVEKAKKRAVVKQTEARNRVELFRHL 291
Query: 903 PQYERGTQLPDLETKFFQLDYIHPAVYKVGLQYLAGDITGGNARCIAMLQAFHKAVEDYS 1082
PQYE GTQLPDLE+KFFQLD +HPAVYKVGLQYL+GD+ GGNARCIAMLQAF + + DYS
Sbjct: 292 PQYEHGTQLPDLESKFFQLDPMHPAVYKVGLQYLSGDVCGGNARCIAMLQAFQEVINDYS 351
Query: 1083 APSEKTVSRDLLAKINSYVSYLIECRPLSVSMGNAIRFLKDHITKLPLDISESEAKASIC 1262
P EKT+ RDL AKI SYVS+LIECRPLS+SMGNAIRFLK+ I KLPL +SE+EAKA++
Sbjct: 352 TPPEKTLVRDLTAKIGSYVSFLIECRPLSISMGNAIRFLKNRIAKLPLTLSEAEAKATLH 411
Query: 1263 SDINRFITEKIVLADRIIVKHAVTKIRDGDVLLTYGSSTVVEMILLQAHVLGTRFRVVVV 1442
+DI+ FI EKI++AD++IVKHAVTKIRDGDVLLTYGSS+ VEMILL AH LG +F+VVVV
Sbjct: 412 ADIDCFIYEKIIVADKVIVKHAVTKIRDGDVLLTYGSSSAVEMILLHAHELGKQFQVVVV 471
Query: 1443 DSRPKLEGQALVRRLVQNGVSCTYTHINAISYIMHEITRVFLGAASVLSNGTVYSRVGTA 1622
DSRPKLEGQ L+RRL+ G+SCTYTHINA+SYIMHE+T+V LGA+SVLSNGTVYSRVGTA
Sbjct: 472 DSRPKLEGQLLLRRLLGKGISCTYTHINAVSYIMHEVTQVVLGASSVLSNGTVYSRVGTA 531
Query: 1623 CVAMVAHEF 1649
CVAM+AH F
Sbjct: 532 CVAMMAHAF 540
>emb|CBI16861.3| unnamed protein product [Vitis vinifera]
Length = 492
Score = 499 bits (1284), Expect = e-138
Identities = 263/396 (66%), Positives = 303/396 (76%), Gaps = 2/396 (0%)
Frame = +3
Query: 471 PQVVSNDEKGGELPARMSLKSDSKPLKEKTTXXXXXXXXXXXXXXXXXXXXXXXXXKTPG 650
P +S G + + + S+ LKEKT+ K P
Sbjct: 2 PPGISVKSGGASIEVQNEQPASSRSLKEKTSKAERRALQEAQRAAKAAAKAEVS--KAPI 59
Query: 651 AATGGEASVQT--GKVVKQPRQKKDGPSMASIEKTVGDRPQEKDKKKDVPPPRMQFDDIG 824
AA+G SV KV K QKKD S+ + EK DRP EKD+KKDVP PRMQFDD
Sbjct: 60 AASGVATSVNAKPAKVSKLSSQKKDSASVTASEKKGVDRPPEKDRKKDVPHPRMQFDDKS 119
Query: 825 RVEKAKKRSVVKQVEATNRVEYFLHLPQYERGTQLPDLETKFFQLDYIHPAVYKVGLQYL 1004
RVEKAK+R+VV+ +E NRVE F HLPQYERGT LPDLE+KFF LD +HPAVYKVGLQYL
Sbjct: 120 RVEKAKRRAVVRPIEVRNRVELFRHLPQYERGTLLPDLESKFFLLDPMHPAVYKVGLQYL 179
Query: 1005 AGDITGGNARCIAMLQAFHKAVEDYSAPSEKTVSRDLLAKINSYVSYLIECRPLSVSMGN 1184
AGD++GGNARCIAMLQAF +A++DYS P EKT+ RDL A+++SYVS+LIECRPLS+SMGN
Sbjct: 180 AGDVSGGNARCIAMLQAFQEAIKDYSTPPEKTLVRDLTARLSSYVSFLIECRPLSISMGN 239
Query: 1185 AIRFLKDHITKLPLDISESEAKASICSDINRFITEKIVLADRIIVKHAVTKIRDGDVLLT 1364
AIRFLK I KLPL +SESEAKAS+ SDINRFI EKIVLAD++IV+HAVTKIRDGDVLLT
Sbjct: 240 AIRFLKSRIAKLPLSLSESEAKASLQSDINRFINEKIVLADKVIVRHAVTKIRDGDVLLT 299
Query: 1365 YGSSTVVEMILLQAHVLGTRFRVVVVDSRPKLEGQALVRRLVQNGVSCTYTHINAISYIM 1544
YGSS+ VEMILL AH LG +FRVVVVDSRPKLEGQ L+RRLV G+SCTYTHINA+SYIM
Sbjct: 300 YGSSSAVEMILLYAHELGKQFRVVVVDSRPKLEGQLLLRRLVGKGLSCTYTHINAVSYIM 359
Query: 1545 HEITRVFLGAASVLSNGTVYSRVGTACVAMVAHEFR 1652
HE+TRVFLGA+SVLSNGTVYSRVGTACV+MVAH FR
Sbjct: 360 HEVTRVFLGASSVLSNGTVYSRVGTACVSMVAHAFR 395