BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00010319
         (1548 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002528285.1| conserved hypothetical protein [Ricinus comm...   606   e-171
ref|XP_002277572.1| PREDICTED: methyltransferase-like protein 16...   602   e-169
ref|XP_002306173.1| predicted protein [Populus trichocarpa] gi|2...   572   e-160
ref|NP_001189567.1| ribosomal RNA large subunit methyltransferas...   529   e-148
ref|NP_179703.2| ribosomal RNA large subunit methyltransferase F...   529   e-148

>ref|XP_002528285.1| conserved hypothetical protein [Ricinus communis]
            gi|223532322|gb|EEF34123.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 473

 Score =  606 bits (1562), Expect = e-171
 Identities = 305/462 (66%), Positives = 345/462 (74%), Gaps = 23/462 (4%)
 Frame = -3

Query: 1513 GGNRKRKRGVDRSIHPRNKYSDNPPDFGLLASLYPSFQSYVFYSRDGRPRIDWTDFNATR 1334
            G N + KR    +IHPRNKY+D+PPDF LLASLYPSF+ ++FYSRDGRPRIDWTDFN+TR
Sbjct: 2    GSNNRNKRRKRATIHPRNKYADSPPDFALLASLYPSFEPFIFYSRDGRPRIDWTDFNSTR 61

Query: 1333 ELTRVLLLHDHQINWWIPDGQLCPTVPNRSNYIHWIEDLLSSDIIPSTRANTDVVKGFDI 1154
            ELTR+LLLHDH +NWWIPDGQLCPTVPNRSNYIHW+EDLLSSDIIP T  N D VKGFDI
Sbjct: 62   ELTRLLLLHDHSLNWWIPDGQLCPTVPNRSNYIHWVEDLLSSDIIPKTNINGDTVKGFDI 121

Query: 1153 GTGANCIYPLLGASLLGWSFVGSDVTDVALEWAQTNVNNNPHVSELIEIRKV-------- 998
            GTGANCIYPLLGASLLGWSFVGSDVTDVA+EWA+ NV NNPHVSELIE+RKV        
Sbjct: 122  GTGANCIYPLLGASLLGWSFVGSDVTDVAVEWAERNVKNNPHVSELIEVRKVTNCQGTLS 181

Query: 997  ------------ESNKDTDNSQDEVCSGMAESYHKLPILLGVVKDGETFDFCMCNPPFFE 854
                        ES  D D S  E      + YH  P+L+GV+K+ E F+FCMCNPPFFE
Sbjct: 182  VEDSRNVESISCESKMDADGSVIEKLVDANKKYHGPPVLVGVIKNDEKFEFCMCNPPFFE 241

Query: 853  TIEEGGLNPKTSCGGTPAEMVCPGGEQAFISRIIKDSVQLKQTFRWYTSMVGRKTNLKTL 674
            ++EE GLNPKTSCGGTP EMVCPGGE+AFI+RII+DSV LK++FRW+TSMVGRKTNLK L
Sbjct: 242  SMEEAGLNPKTSCGGTPEEMVCPGGEKAFIARIIEDSVILKESFRWFTSMVGRKTNLKFL 301

Query: 673  TSKLREVGVTIVKTTEFVQGQTCRWGLAWSFVPPSTKIVKSHI-TKSDISFMLEGIQRQY 497
            TS+LREVGVTIVKTTEFVQGQTCRWGLAWSFVPP+ KI+  H+  KS I FMLEG+QRQ+
Sbjct: 302  TSRLREVGVTIVKTTEFVQGQTCRWGLAWSFVPPARKIILPHVDDKSIIPFMLEGLQRQF 361

Query: 496  SAIDVLQXXXXXXXXXXXXXXSDIVSFHINIRASKYLCDAILKNETVNTVEATNCQ-LE- 323
            SAIDVLQ               +  SF ++I AS   CDAILKNE   +    NC  LE 
Sbjct: 362  SAIDVLQSVESFFRTTGASCRLNSTSFAVDITASMDQCDAILKNEAKESDGTANCDPLEG 421

Query: 322  HRXXXXXXXXXXXXXXXNFRISVFQQIPGTLLVKGSLQQKEN 197
                              FRI VFQQIPGTLLVKGSLQ K++
Sbjct: 422  TSKSNSSTLQHPPLNKLCFRILVFQQIPGTLLVKGSLQHKDS 463


>ref|XP_002277572.1| PREDICTED: methyltransferase-like protein 16-like [Vitis vinifera]
          Length = 497

 Score =  602 bits (1551), Expect = e-169
 Identities = 308/496 (62%), Positives = 360/496 (72%), Gaps = 34/496 (6%)
 Frame = -3

Query: 1510 GNRKRKRGVDRSIHPRNKYSDNPPDFGLLASLYPSFQSYVFYSRDGRPRIDWTDFNATRE 1331
            G +KR+R    SIHPRNKYS+NPPDF LLASLYPSF+ +VF +R GRP IDWTDFNATRE
Sbjct: 2    GGKKRRRVERPSIHPRNKYSENPPDFALLASLYPSFKPFVFLNRAGRPTIDWTDFNATRE 61

Query: 1330 LTRVLLLHDHQINWWIPDGQLCPTVPNRSNYIHWIEDLLSSDIIPSTRANTDVVKGFDIG 1151
            LTRVLLLHDH +NWWIPDGQLCPTVPNRSNYIHWIEDLLSSDIIP T  + + V+GFDIG
Sbjct: 62   LTRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWIEDLLSSDIIPKTSTDGNNVRGFDIG 121

Query: 1150 TGANCIYPLLGASLLGWSFVGSDVTDVALEWAQTNVNNNPHVSELIEIRKVESNKDTDNS 971
            TGANCIYPLLGASLLGWSFVG+DVTDVALEWA+ NV +NPH+SELIEIRKVES+  T + 
Sbjct: 122  TGANCIYPLLGASLLGWSFVGTDVTDVALEWAEKNVKSNPHISELIEIRKVESDGSTPSG 181

Query: 970  QD--------------------------EVCS-----GMAESYHKLPILLGVVKDGETFD 884
            +                             CS     G  +SYH  P+LLG++KDGE  D
Sbjct: 182  EGLHIGESFIVEGEKDLSGINAGDTEPLPSCSLDADLGSNKSYHGPPVLLGILKDGENLD 241

Query: 883  FCMCNPPFFETIEEGGLNPKTSCGGTPAEMVCPGGEQAFISRIIKDSVQLKQTFRWYTSM 704
            FCMCNPPFFET+EE GLNPKTSCGGTP EMVCPGGE+AFI+RII DSV LKQ+FRWYTSM
Sbjct: 242  FCMCNPPFFETMEEAGLNPKTSCGGTPEEMVCPGGEKAFITRIIDDSVILKQSFRWYTSM 301

Query: 703  VGRKTNLKTLTSKLREVGVTIVKTTEFVQGQTCRWGLAWSFVPPSTKIVKSHIT-KSDIS 527
            VG+KTNLK L SKLR VGVTIVKTTEFVQGQTCRWG+AWSFVP + K++  H+  K++ S
Sbjct: 302  VGKKTNLKILVSKLRNVGVTIVKTTEFVQGQTCRWGIAWSFVPSARKLILPHVAKKNNQS 361

Query: 526  FMLEGIQRQYSAIDVLQXXXXXXXXXXXXXXSDIVSFHINIRASKYLCDAILKNETVNTV 347
            FMLEG+QRQ+SAI+VLQ              S+  SF +++  S   C+AILK E     
Sbjct: 362  FMLEGLQRQFSAINVLQSVESFFCTIGASCKSNASSFTVDVAISNDHCNAILKTEVQGYN 421

Query: 346  EATNCQLEHRXXXXXXXXXXXXXXXNFRISVFQQIPGTLLVKGSLQQKEN--QGILLLVF 173
            E  N Q   +                FRI VFQQIPGTLLV+GSL+ +E+   G+   +F
Sbjct: 422  ELNNYQY-MQEICDGSSFPPSFEELCFRILVFQQIPGTLLVRGSLKDRESPGSGVFWSIF 480

Query: 172  QHLEDILRNQFCRGKA 125
            Q LE++L+N+FCR KA
Sbjct: 481  QQLEEVLKNKFCREKA 496


>ref|XP_002306173.1| predicted protein [Populus trichocarpa] gi|222849137|gb|EEE86684.1|
            predicted protein [Populus trichocarpa]
          Length = 443

 Score =  572 bits (1473), Expect = e-160
 Identities = 289/441 (65%), Positives = 328/441 (74%), Gaps = 11/441 (2%)
 Frame = -3

Query: 1501 KRKRGVDRSIHPRNKYSDNPPDFGLLASLYPSFQSYVFYSRDGRPRIDWTDFNATRELTR 1322
            KRKR    +IHP+NKYSDNPPDF +LASLYPS + +VFYSRDGRPRIDWTD+N+TRELTR
Sbjct: 4    KRKRPERPTIHPKNKYSDNPPDFSVLASLYPSLKPFVFYSRDGRPRIDWTDYNSTRELTR 63

Query: 1321 VLLLHDHQINWWIPDGQLCPTVPNRSNYIHWIEDLLSSDIIPSTRANTDVVKGFDIGTGA 1142
            VLLLHDH +NWWIPDGQLCPTVPNRSNYIHWIEDLLSSDIIP    N D+V+GFDIGTGA
Sbjct: 64   VLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWIEDLLSSDIIPKNNNNDDIVRGFDIGTGA 123

Query: 1141 NCIYPLLGASLLGWSFVGSDVTDVALEWAQTNVNNNPHVSELIEIRKVESNKDTDNSQDE 962
            NCIYPLLGASLLGW+FVGSDVTDVA+EWA+ NV  N H+SELIEIRKV   +   + +D 
Sbjct: 124  NCIYPLLGASLLGWTFVGSDVTDVAVEWAEKNVKCNQHISELIEIRKVTDCQSAISIEDS 183

Query: 961  VC----------SGMAESYHKLPILLGVVKDGETFDFCMCNPPFFETIEEGGLNPKTSCG 812
             C           G A  Y   P+LLGVV+DGE FDFCMCNPPFFET+EE GLNPKTSCG
Sbjct: 184  NCGESVNCENKMDGNATKYSGPPLLLGVVRDGEKFDFCMCNPPFFETMEEAGLNPKTSCG 243

Query: 811  GTPAEMVCPGGEQAFISRIIKDSVQLKQTFRWYTSMVGRKTNLKTLTSKLREVGVTIVKT 632
            GTP EMVCPGGE+AFI+ II+DSV LK++FRW+TSMVGRK NLK LTSKLREVGVTIVKT
Sbjct: 244  GTPEEMVCPGGEKAFITCIIEDSVVLKESFRWFTSMVGRKVNLKFLTSKLREVGVTIVKT 303

Query: 631  TEFVQGQTCRWGLAWSFVPPSTKIVKSHITKSDISFMLE-GIQRQYSAIDVLQXXXXXXX 455
            TEFVQGQTCRWGLAWSFVPP  KI+   + K+ +SF LE G+QRQ+SA  VLQ       
Sbjct: 304  TEFVQGQTCRWGLAWSFVPPVRKIISPLVQKNSLSFTLEQGLQRQFSAFHVLQSIESFFC 363

Query: 454  XXXXXXXSDIVSFHINIRASKYLCDAILKNETVNTVEATNCQLEHRXXXXXXXXXXXXXX 275
                    +  SF ++I AS   C AIL++E  +   A  C                   
Sbjct: 364  LGGASCKLNASSFVVDITASNDQCKAILRDEAKHPNGAATCN-PLEASHSSSYLHPPSNN 422

Query: 274  XNFRISVFQQIPGTLLVKGSL 212
             +F ISVFQQIPGTLLVK SL
Sbjct: 423  LSFCISVFQQIPGTLLVKASL 443


>ref|NP_001189567.1| ribosomal RNA large subunit methyltransferase F [Arabidopsis
            thaliana] gi|330252026|gb|AEC07120.1| ribosomal RNA large
            subunit methyltransferase F [Arabidopsis thaliana]
          Length = 513

 Score =  529 bits (1363), Expect = e-148
 Identities = 281/485 (57%), Positives = 336/485 (69%), Gaps = 23/485 (4%)
 Frame = -3

Query: 1519 MGGGNRKRKRGVDRSIHPRNKYSDNPPDFGLLASLYPSFQSYVFYSRDGRPRIDWTDFNA 1340
            M  G ++ +     +  PR+KYSDNPPDF  LASLYPSF+ +VF+S   R RIDWTD+NA
Sbjct: 1    MRSGKKRARSEPGSTKQPRSKYSDNPPDFASLASLYPSFKPFVFFS-GSRARIDWTDYNA 59

Query: 1339 TRELTRVLLLHDHQINWWIPDGQLCPTVPNRSNYIHWIEDLLSSDIIPSTRANTDVVKGF 1160
            TRELTRVLLLHDH +NWWIPDGQLCPTVPNRSNYIHWI DLLSS II S   +   VKGF
Sbjct: 60   TRELTRVLLLHDHGVNWWIPDGQLCPTVPNRSNYIHWINDLLSSGIIQSLGGDGSKVKGF 119

Query: 1159 DIGTGANCIYPLLGASLLGWSFVGSDVTDVALEWAQTNVNNNPHVSELIEIR-------- 1004
            DIGTGANCIYPLLGASL GWSFVGSD T VALEWA+ NV +NPH S+LIEIR        
Sbjct: 120  DIGTGANCIYPLLGASLFGWSFVGSDFTVVALEWAEKNVQSNPHFSDLIEIRDSKVPPQC 179

Query: 1003 ----KVES--NKDTDNSQDEVCSGMAESYH------KLPILLGVVKDGETFDFCMCNPPF 860
                +VE+   + T   + E+ + +   YH      +  +LLGVVK+ ETFDFCM NPPF
Sbjct: 180  SSVPEVENTEREKTIQEEAEISATVKSDYHDNKSFIEPAVLLGVVKENETFDFCMSNPPF 239

Query: 859  FETIEEGGLNPKTSCGGTPAEMVCPGGEQAFISRIIKDSVQLKQTFRWYTSMVGRKTNLK 680
            FET EE GLNPKTSCGGTP EMVC GGEQAF+SRIIKDS  L+Q FRWYTSM+G+K NLK
Sbjct: 240  FETFEEAGLNPKTSCGGTPEEMVCNGGEQAFVSRIIKDSAVLRQRFRWYTSMLGKKANLK 299

Query: 679  TLTSKLREVGVTIVKTTEFVQGQTCRWGLAWSFVPPSTKIVKSHIT-KSDISFMLEGIQR 503
             L SKL EVGVTIVKTTEFVQGQT RWGLAWSF+P + KI+   +  KS +SFMLE I+R
Sbjct: 300  LLISKLWEVGVTIVKTTEFVQGQTSRWGLAWSFMPIARKIIAPPVVKKSVLSFMLECIKR 359

Query: 502  QYSAIDVLQXXXXXXXXXXXXXXSDIVSFHINIRASKYLCDAILKNETVNTVEATNCQLE 323
            QYSA+DVLQ               +  +F ++I AS   C+ I KN+ +  V++      
Sbjct: 360  QYSAVDVLQSVEEFFKSCGASCKLNSSTFSVDIVASNDQCNTISKND-IADVDSVRSHGY 418

Query: 322  HRXXXXXXXXXXXXXXXNFRISVFQQIPGTLLVKGSLQQKEN--QGILLLVFQHLEDILR 149
             +               +FRI VFQQ+PGTLL+KGSLQQK++   G+   VF  LE+ L+
Sbjct: 419  EKQSLDGSSLQVPEYNLSFRILVFQQMPGTLLIKGSLQQKDSPLSGLFSRVFGSLEESLK 478

Query: 148  NQFCR 134
            ++FCR
Sbjct: 479  SKFCR 483


>ref|NP_179703.2| ribosomal RNA large subunit methyltransferase F [Arabidopsis
            thaliana] gi|53850469|gb|AAU95411.1| At2g21070
            [Arabidopsis thaliana] gi|55733759|gb|AAV59276.1|
            At2g21070 [Arabidopsis thaliana]
            gi|330252024|gb|AEC07118.1| ribosomal RNA large subunit
            methyltransferase F [Arabidopsis thaliana]
          Length = 483

 Score =  529 bits (1363), Expect = e-148
 Identities = 281/485 (57%), Positives = 336/485 (69%), Gaps = 23/485 (4%)
 Frame = -3

Query: 1519 MGGGNRKRKRGVDRSIHPRNKYSDNPPDFGLLASLYPSFQSYVFYSRDGRPRIDWTDFNA 1340
            M  G ++ +     +  PR+KYSDNPPDF  LASLYPSF+ +VF+S   R RIDWTD+NA
Sbjct: 1    MRSGKKRARSEPGSTKQPRSKYSDNPPDFASLASLYPSFKPFVFFS-GSRARIDWTDYNA 59

Query: 1339 TRELTRVLLLHDHQINWWIPDGQLCPTVPNRSNYIHWIEDLLSSDIIPSTRANTDVVKGF 1160
            TRELTRVLLLHDH +NWWIPDGQLCPTVPNRSNYIHWI DLLSS II S   +   VKGF
Sbjct: 60   TRELTRVLLLHDHGVNWWIPDGQLCPTVPNRSNYIHWINDLLSSGIIQSLGGDGSKVKGF 119

Query: 1159 DIGTGANCIYPLLGASLLGWSFVGSDVTDVALEWAQTNVNNNPHVSELIEIR-------- 1004
            DIGTGANCIYPLLGASL GWSFVGSD T VALEWA+ NV +NPH S+LIEIR        
Sbjct: 120  DIGTGANCIYPLLGASLFGWSFVGSDFTVVALEWAEKNVQSNPHFSDLIEIRDSKVPPQC 179

Query: 1003 ----KVES--NKDTDNSQDEVCSGMAESYH------KLPILLGVVKDGETFDFCMCNPPF 860
                +VE+   + T   + E+ + +   YH      +  +LLGVVK+ ETFDFCM NPPF
Sbjct: 180  SSVPEVENTEREKTIQEEAEISATVKSDYHDNKSFIEPAVLLGVVKENETFDFCMSNPPF 239

Query: 859  FETIEEGGLNPKTSCGGTPAEMVCPGGEQAFISRIIKDSVQLKQTFRWYTSMVGRKTNLK 680
            FET EE GLNPKTSCGGTP EMVC GGEQAF+SRIIKDS  L+Q FRWYTSM+G+K NLK
Sbjct: 240  FETFEEAGLNPKTSCGGTPEEMVCNGGEQAFVSRIIKDSAVLRQRFRWYTSMLGKKANLK 299

Query: 679  TLTSKLREVGVTIVKTTEFVQGQTCRWGLAWSFVPPSTKIVKSHIT-KSDISFMLEGIQR 503
             L SKL EVGVTIVKTTEFVQGQT RWGLAWSF+P + KI+   +  KS +SFMLE I+R
Sbjct: 300  LLISKLWEVGVTIVKTTEFVQGQTSRWGLAWSFMPIARKIIAPPVVKKSVLSFMLECIKR 359

Query: 502  QYSAIDVLQXXXXXXXXXXXXXXSDIVSFHINIRASKYLCDAILKNETVNTVEATNCQLE 323
            QYSA+DVLQ               +  +F ++I AS   C+ I KN+ +  V++      
Sbjct: 360  QYSAVDVLQSVEEFFKSCGASCKLNSSTFSVDIVASNDQCNTISKND-IADVDSVRSHGY 418

Query: 322  HRXXXXXXXXXXXXXXXNFRISVFQQIPGTLLVKGSLQQKEN--QGILLLVFQHLEDILR 149
             +               +FRI VFQQ+PGTLL+KGSLQQK++   G+   VF  LE+ L+
Sbjct: 419  EKQSLDGSSLQVPEYNLSFRILVFQQMPGTLLIKGSLQQKDSPLSGLFSRVFGSLEESLK 478

Query: 148  NQFCR 134
            ++FCR
Sbjct: 479  SKFCR 483