BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00010283
         (1818 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002281142.1| PREDICTED: mitogen-activated protein kinase ...   518   e-144
gb|AAS19278.1| mitogen-activated protein kinase kinase kinase [V...   516   e-144
dbj|BAA05648.1| protein kinase [Nicotiana tabacum]                    494   e-137
ref|XP_002311605.1| predicted protein [Populus trichocarpa] gi|2...   494   e-137
ref|XP_002517199.1| mitogen activated protein kinase kinase kina...   483   e-134

>ref|XP_002281142.1| PREDICTED: mitogen-activated protein kinase kinase kinase 3 [Vitis
            vinifera] gi|297737007|emb|CBI26208.3| unnamed protein
            product [Vitis vinifera]
          Length = 686

 Score =  518 bits (1333), Expect = e-144
 Identities = 279/486 (57%), Positives = 339/486 (69%), Gaps = 4/486 (0%)
 Frame = -3

Query: 1816 HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWMAPEVILQ 1637
            HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWMAPEVILQ
Sbjct: 187  HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWMAPEVILQ 246

Query: 1636 TGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPDHLSAEAKDFL 1457
            TGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP+HL+AEAKDFL
Sbjct: 247  TGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEHLTAEAKDFL 306

Query: 1456 LKCLHKEPDLRPTASVLLQHPFVTGEYHESHYVNRTSIKQDHSGKHMEISRSNQRNSINP 1277
            LKCL KEP+LRP AS LLQHPFV+GEY E H V +TS+  ++SG  M  S ++ ++  NP
Sbjct: 307  LKCLQKEPNLRPAASELLQHPFVSGEYQEPHPVFQTSV-MENSGNMMATSETDLKSFPNP 365

Query: 1276 G-KRPFGTGLHEVHDMGSVRCSTIYPEKSLGDAPNWGSSNYXXXXXXXXXXXXXXDLFSA 1100
              +R   T  + V +MGSVRCST+YPEK  G  P WG+++               D    
Sbjct: 366  VIRRSNCTSSNNVCNMGSVRCSTVYPEKFSGTGPLWGANS---CDDDMCQIDDKDDFIVH 422

Query: 1099 VSMKPXXXXXXXXXXXXXNPMCEPNDDWQCKFDDSPVMERSATNIYPSEFTNRHSKIFES 920
             S+K              NPMCEP DDW CKFD+SP + +S  N+   +  ++ +    +
Sbjct: 423  QSVKFGSAILSDHLNKSFNPMCEPTDDWPCKFDESPELTKSGANLSSHQTISKPAGSPRA 482

Query: 919  SGNVDNDFTFPSGTVXXXXXXXXXESKIKAFLDEKALDLKKLQSPLYEEFYNS-MNPMIP 743
            S   +NDFTFP G +         ESKI+AFLDEKALDLKKLQ+PLYEEFYNS +N   P
Sbjct: 483  SNERENDFTFPCGPLVGDDDDEVTESKIRAFLDEKALDLKKLQTPLYEEFYNSTLNAAGP 542

Query: 742  SAVGTENKENVAYNFNLPPKSRSPNRVPSRRFSAAVDVACTASPGSRSRHVSDGGCSTHQ 563
             +   ++ +NV    NLPPKSRSPN+ P+RR SAAVD AC ++  + ++   + G  + +
Sbjct: 543  PSAVGKSHDNVTNFLNLPPKSRSPNQTPTRRLSAAVDAACISNSANHTKRGLNVGTISDR 602

Query: 562  NTREVKSPPHVELKE-VLDSRQEPISLSASFSERQRKWXXXXXXXXXXXXXXMRQAGM-A 389
              +E++SP   E KE +LD++QEP+SLS +FSER+RKW              +RQAG+  
Sbjct: 603  TLKEIQSPQLSEWKELLLDAQQEPVSLSTNFSERRRKWKEELDQELEKKRELLRQAGVGG 662

Query: 388  NTSSPK 371
             T+SPK
Sbjct: 663  KTASPK 668


>gb|AAS19278.1| mitogen-activated protein kinase kinase kinase [Vitis vinifera]
          Length = 686

 Score =  516 bits (1329), Expect = e-144
 Identities = 280/486 (57%), Positives = 339/486 (69%), Gaps = 4/486 (0%)
 Frame = -3

Query: 1816 HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWMAPEVILQ 1637
            HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPY MAPEVILQ
Sbjct: 187  HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYRMAPEVILQ 246

Query: 1636 TGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPDHLSAEAKDFL 1457
            TGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP+HL+AEAKDFL
Sbjct: 247  TGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEHLTAEAKDFL 306

Query: 1456 LKCLHKEPDLRPTASVLLQHPFVTGEYHESHYVNRTSIKQDHSGKHMEISRSNQRNSINP 1277
            LKCL KEP+LRP AS LLQHPFV+GEY E H V +TS+  ++SG  M  S ++ ++  NP
Sbjct: 307  LKCLQKEPNLRPAASELLQHPFVSGEYQEPHPVFQTSV-MENSGNMMATSETDLKSFPNP 365

Query: 1276 G-KRPFGTGLHEVHDMGSVRCSTIYPEKSLGDAPNWGSSNYXXXXXXXXXXXXXXDLFSA 1100
              +R   T  + V +MGSVRCST+YPEK  G  P WG+++               D    
Sbjct: 366  VIRRSNCTSSNNVCNMGSVRCSTVYPEKFSGTGPLWGANS---CDDDMCQIDDKDDFIVH 422

Query: 1099 VSMKPXXXXXXXXXXXXXNPMCEPNDDWQCKFDDSPVMERSATNIYPSEFTNRHSKIFES 920
             S+K              NPMCEP DDW CKFD+SP + +S  N+   +  ++ +    +
Sbjct: 423  QSVKFGSAILSDHLNKSFNPMCEPTDDWPCKFDESPELTKSGANLSSHQTISKPAGSPRA 482

Query: 919  SGNVDNDFTFPSGTVXXXXXXXXXESKIKAFLDEKALDLKKLQSPLYEEFYNS-MNPMIP 743
            S   +NDFTFP G +         ESKI+AFLDEKALDLKKLQ+PLYEEFYNS +N   P
Sbjct: 483  SNERENDFTFPCGPLVGDDDDEVTESKIRAFLDEKALDLKKLQTPLYEEFYNSTLNAAGP 542

Query: 742  SAVGTENKENVAYNFNLPPKSRSPNRVPSRRFSAAVDVACTASPGSRSRHVSDGGCSTHQ 563
             +   ++ +NV    NLPPKSRSPNR P+RR SAAVD ACT++  + ++   + G  + +
Sbjct: 543  PSAVGKSHDNVTNFLNLPPKSRSPNRTPTRRLSAAVDAACTSNSANHTKRGLNVGTISDR 602

Query: 562  NTREVKSPPHVELKE-VLDSRQEPISLSASFSERQRKWXXXXXXXXXXXXXXMRQAGM-A 389
              +E++SP   E KE +LD++QEP+SLS +FSER+RKW              +RQAG+  
Sbjct: 603  TLKEIQSPQLSEWKELLLDAQQEPVSLSTNFSERRRKWKEELDQELEKKRELLRQAGVGG 662

Query: 388  NTSSPK 371
             T+SPK
Sbjct: 663  KTASPK 668


>dbj|BAA05648.1| protein kinase [Nicotiana tabacum]
          Length = 690

 Score =  494 bits (1271), Expect = e-137
 Identities = 275/483 (56%), Positives = 325/483 (67%), Gaps = 1/483 (0%)
 Frame = -3

Query: 1816 HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWMAPEVILQ 1637
            HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWMAPEVILQ
Sbjct: 200  HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWMAPEVILQ 259

Query: 1636 TGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPDHLSAEAKDFL 1457
            TGHSFSADIWSVGCT+IEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP+HLSAE+KDFL
Sbjct: 260  TGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEHLSAESKDFL 319

Query: 1456 LKCLHKEPDLRPTASVLLQHPFVTGEYHESHYVNRTSIKQDHSGKHMEISRSNQRNSINP 1277
            LKCL KEP LR +AS LLQHPFVT E+ E+    R+S     + ++M   R + R SI P
Sbjct: 320  LKCLQKEPHLRHSASNLLQHPFVTAEHQEARPFLRSSFM--GNPENMAAQRMDVRTSIIP 377

Query: 1276 GKRPFGTGLHEVHDMGSVRCSTIYPEKSLGDAPNWGSSNYXXXXXXXXXXXXXXDLFSAV 1097
              R    GL +V  + +VRCST+YPE SLG    W   N                +F A 
Sbjct: 378  DMRASCNGLKDVCGVSAVRCSTVYPENSLGKESLWKLGN---SDDDMCQMDNDDFMFGA- 433

Query: 1096 SMKPXXXXXXXXXXXXXNPMCEPNDDWQCKFDDSPVMERSATNIYPSEFTNRHSKIFESS 917
            S+K              NPMCEP++DW CKFD+SP + +S  N++  + T + +     S
Sbjct: 434  SVKCSSDLHSPANYKSFNPMCEPDNDWPCKFDESPELTKSQANLHYDQATIKPTNNPIMS 493

Query: 916  GNVDNDFTFPSGTVXXXXXXXXXESKIKAFLDEKALDLKKLQSPLYEEFYNSMN-PMIPS 740
               D  FTFPSG           ESKI+AFLDEKA+DLKKLQ+PLYE FYNS+N    PS
Sbjct: 494  YKEDLAFTFPSGQSAAEDDDELTESKIRAFLDEKAMDLKKLQTPLYEGFYNSLNVSSTPS 553

Query: 739  AVGTENKENVAYNFNLPPKSRSPNRVPSRRFSAAVDVACTASPGSRSRHVSDGGCSTHQN 560
             VGT NKENV  N NLPPKSRSP R+ SRR S A++ AC  SP + S+ +S+ G    + 
Sbjct: 554  PVGTGNKENVPSNINLPPKSRSPKRMLSRRLSTAIEGACAPSPVTHSKRISNIGGLNGEA 613

Query: 559  TREVKSPPHVELKEVLDSRQEPISLSASFSERQRKWXXXXXXXXXXXXXXMRQAGMANTS 380
             +E + P H E K++L S++E  ++++SFSERQR+W              MRQA   N S
Sbjct: 614  IQEAQLPRHNEWKDLLGSQRE--AVNSSFSERQRRWKEELDEELQRKREIMRQA--VNLS 669

Query: 379  SPK 371
             PK
Sbjct: 670  PPK 672


>ref|XP_002311605.1| predicted protein [Populus trichocarpa] gi|222851425|gb|EEE88972.1|
            predicted protein [Populus trichocarpa]
          Length = 653

 Score =  494 bits (1271), Expect = e-137
 Identities = 269/458 (58%), Positives = 316/458 (68%), Gaps = 3/458 (0%)
 Frame = -3

Query: 1816 HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWMAPEVILQ 1637
            HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELAT+ GAKSMKGTPYWMAPEVILQ
Sbjct: 189  HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVILQ 248

Query: 1636 TGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPDHLSAEAKDFL 1457
            TGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP+HLS EAKDFL
Sbjct: 249  TGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEHLSIEAKDFL 308

Query: 1456 LKCLHKEPDLRPTASVLLQHPFVTGEYHESHYVNRTSIKQDHSGKHMEISRSNQRNSINP 1277
            LKCL + P+LRP AS LLQHPFVTGEY E+H V R S++         I+    R+  + 
Sbjct: 309  LKCLQEVPNLRPAASELLQHPFVTGEYQETHSVFRNSVRVSVVRCAGLITMYRWRSMNSV 368

Query: 1276 GKRPFGTGLHEVHDMGSVRCSTIYPEKSLGDAPNWGSSNYXXXXXXXXXXXXXXDLFSAV 1097
             +R    GL +V +MGSV+ ST+Y +        WG++N+                  + 
Sbjct: 369  IRRSTCAGLKDVCEMGSVKSSTVYRDNLSRSRSYWGAANFDDDMCLIDDKDDFAV---SA 425

Query: 1096 SMKPXXXXXXXXXXXXXNPMCEPNDDWQCKFDDSPVMERSATNIYPSEFTNRHSKIFESS 917
            S +              NPMCEP DDW CKFD+ P+ +RS  N+  S+  +    I E+S
Sbjct: 426  STRFKSTLASADLNKSVNPMCEPLDDWPCKFDEDPLFKRSGYNLSCSQSNHEAIDIHEAS 485

Query: 916  GNVDNDFTFPSGTVXXXXXXXXXESKIKAFLDEKALDLKKLQSPLYEEFYNS-MNPM-IP 743
            G   NDFTFP G V         ESKI+AFLDEKALDLKKLQ+PLYEEFYNS +N M  P
Sbjct: 486  GKGQNDFTFPCGPVVPEDDDEVTESKIRAFLDEKALDLKKLQTPLYEEFYNSTLNTMGAP 545

Query: 742  SAVGTENKENVAYNFNLPPKSRSPNRVPSRRFSAAVDVACTASPGSRSRHVSDGGCSTHQ 563
            +AVGTEN EN  +  +LPPKSRSP R+PSRR SA VD    ASPG ++ HV++     ++
Sbjct: 546  TAVGTENSENPTHLPSLPPKSRSPKRLPSRRLSAVVDAPSIASPGRQTNHVANESSIHNR 605

Query: 562  NTREVKSPPHVELKEVL-DSRQEPISLSASFSERQRKW 452
              +E++ P   E KE L D +QE  +L+ SFSERQRKW
Sbjct: 606  ALQEIQPPQLSEWKEFLHDGQQE--TLTTSFSERQRKW 641


>ref|XP_002517199.1| mitogen activated protein kinase kinase kinase 3, mapkkk3, mekk3,
            putative [Ricinus communis] gi|223543834|gb|EEF45362.1|
            mitogen activated protein kinase kinase kinase 3,
            mapkkk3, mekk3, putative [Ricinus communis]
          Length = 653

 Score =  483 bits (1244), Expect = e-134
 Identities = 268/457 (58%), Positives = 313/457 (68%), Gaps = 2/457 (0%)
 Frame = -3

Query: 1816 HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWMAPEVILQ 1637
            HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELAT+ GAKSMKGTPYWMAPEVILQ
Sbjct: 182  HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVILQ 241

Query: 1636 TGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPDHLSAEAKDFL 1457
            TGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP+HLS EAKDFL
Sbjct: 242  TGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEHLSIEAKDFL 301

Query: 1456 LKCLHKEPDLRPTASVLLQHPFVTGEYHESHYVNRTSIKQDHSGKHMEISRSNQRNSINP 1277
            LKCL KEP+LRPTA  LLQHPFVTGEYHE H V R+S+++  SG  M  +  N +NS+NP
Sbjct: 302  LKCLQKEPNLRPTAFDLLQHPFVTGEYHEPHLVFRSSVRE--SGNLMATTGLNIKNSVNP 359

Query: 1276 GKRPFGTGLHEVHDMGSVRCSTIYPEKSLGDAPNWGSSNYXXXXXXXXXXXXXXDLFSAV 1097
                   GL +V  M S R S  YP         WG +N               D   + 
Sbjct: 360  VIMSACAGLKDVCGMSSSRSSIRYPNNITRSGFTWGETN---IDDDMCQIDDKDDFVVSP 416

Query: 1096 SMKPXXXXXXXXXXXXXNPMCEPNDDWQCKFDDSPVMERSATNIYPSEFTNRHSKIFESS 917
            S +              NPMCEP D+W CKFD+SP  +R   N+  S+  +  + I   S
Sbjct: 417  SARFKSAFASADLNKSFNPMCEPIDEWTCKFDESP--KRGGINLSSSQSLHDIADIPAES 474

Query: 916  GNVDNDFTFPSGTVXXXXXXXXXESKIKAFLDEKALDLKKLQSPLYEEFYNSMNPM-IPS 740
            G  +NDFTFP G V         ESKI+AFLDEKALDLKKLQ+PLYEEFYN++N   + S
Sbjct: 475  GKEENDFTFPCGPVATEDDDEVTESKIRAFLDEKALDLKKLQTPLYEEFYNTLNAAGLAS 534

Query: 739  AVGTENKENVAYNFNLPPKSRSPNRVPSRRFSAAVDVACTASPGSRSRHVSDGGCSTHQN 560
            AVG+ N EN+     LPPKSRSP R+PSRRFS  ++ A TAS  S ++ + +      + 
Sbjct: 535  AVGSTNTENITNLPTLPPKSRSPKRLPSRRFS-IIEAANTASTRSSNKQILNDSSIHDRA 593

Query: 559  TREVKSPPHVELK-EVLDSRQEPISLSASFSERQRKW 452
             +E++ P   E K  +LD+++E I+ SASFSERQRKW
Sbjct: 594  LQEIQPPQVNEWKGMLLDAQKEAITPSASFSERQRKW 630