BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00010264
         (737 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI28880.3| unnamed protein product [Vitis vinifera]              142   5e-32
ref|XP_002271752.1| PREDICTED: triacylglycerol lipase 2 [Vitis v...   142   5e-32
ref|XP_002528965.1| Triacylglycerol lipase 2 precursor, putative...   137   2e-30
ref|XP_002320493.1| predicted protein [Populus trichocarpa] gi|2...   136   4e-30
emb|CBI28874.3| unnamed protein product [Vitis vinifera]              135   7e-30

>emb|CBI28880.3| unnamed protein product [Vitis vinifera]
          Length = 483

 Score =  142 bits (359), Expect = 5e-32
 Identities = 73/121 (60%), Positives = 86/121 (71%), Gaps = 18/121 (14%)
 Frame = +1

Query: 427 VWIANTRGNNYSRGHTSLAPSDPAYWDWSWDELVAYEF------------------GHSL 552
           VW+ANTRG  YSRGHT+L+P+  AYWDWSWD+LVAY+                   GHSL
Sbjct: 198 VWLANTRGTRYSRGHTTLSPNKSAYWDWSWDQLVAYDLSATFQYVSDQTGQKLHYVGHSL 257

Query: 553 GTLIAFASLSQDKNLNMLHSAALLSLIAYLGQMSSFLARSAADIFLAEELKWPGLNEFNL 732
           GTLIA A+ SQ+K +NML SAALLS IAYL QMSS LAR+A DIFLAE+L W G+ EF+ 
Sbjct: 258 GTLIALAAFSQEKLVNMLRSAALLSPIAYLNQMSSLLARTAVDIFLAEDLYWLGVYEFDP 317

Query: 733 R 735
           R
Sbjct: 318 R 318



 Score = 81.3 bits (199), Expect = 2e-13
 Identities = 43/94 (45%), Positives = 55/94 (58%), Gaps = 5/94 (5%)
 Frame = +3

Query: 15  FNRARAAKLTLPHYVCHTPLFMPFGTQEVAVRTKLSSVNE*S-----GICKLMDDTQGYL 179
           F   +  ++ L +    + L +       A RTK+ S+N        GICK M + QGY 
Sbjct: 73  FTIPKKVRIRLENISASSLLLILLFVSAAASRTKIYSINNEDVHATDGICKTMVEKQGYA 132

Query: 180 CEEHTVITEDGYILSMQRIPKGRSGRASDKPPVL 281
           C+EH V T+DGYILSMQRIPKG+SG   DKPPVL
Sbjct: 133 CQEHLVTTQDGYILSMQRIPKGQSGEVPDKPPVL 166


>ref|XP_002271752.1| PREDICTED: triacylglycerol lipase 2 [Vitis vinifera]
          Length = 401

 Score =  142 bits (359), Expect = 5e-32
 Identities = 73/121 (60%), Positives = 86/121 (71%), Gaps = 18/121 (14%)
 Frame = +1

Query: 427 VWIANTRGNNYSRGHTSLAPSDPAYWDWSWDELVAYEF------------------GHSL 552
           VW+ANTRG  YSRGHT+L+P+  AYWDWSWD+LVAY+                   GHSL
Sbjct: 116 VWLANTRGTRYSRGHTTLSPNKSAYWDWSWDQLVAYDLSATFQYVSDQTGQKLHYVGHSL 175

Query: 553 GTLIAFASLSQDKNLNMLHSAALLSLIAYLGQMSSFLARSAADIFLAEELKWPGLNEFNL 732
           GTLIA A+ SQ+K +NML SAALLS IAYL QMSS LAR+A DIFLAE+L W G+ EF+ 
Sbjct: 176 GTLIALAAFSQEKLVNMLRSAALLSPIAYLNQMSSLLARTAVDIFLAEDLYWLGVYEFDP 235

Query: 733 R 735
           R
Sbjct: 236 R 236



 Score = 80.5 bits (197), Expect = 3e-13
 Identities = 40/65 (61%), Positives = 46/65 (70%), Gaps = 5/65 (7%)
 Frame = +3

Query: 102 AVRTKLSSVNE*S-----GICKLMDDTQGYLCEEHTVITEDGYILSMQRIPKGRSGRASD 266
           A RTK+ S+N        GICK M + QGY C+EH V T+DGYILSMQRIPKG+SG   D
Sbjct: 20  ASRTKIYSINNEDVHATDGICKTMVEKQGYACQEHLVTTQDGYILSMQRIPKGQSGEVPD 79

Query: 267 KPPVL 281
           KPPVL
Sbjct: 80  KPPVL 84


>ref|XP_002528965.1| Triacylglycerol lipase 2 precursor, putative [Ricinus communis]
           gi|223531611|gb|EEF33439.1| Triacylglycerol lipase 2
           precursor, putative [Ricinus communis]
          Length = 274

 Score =  137 bits (346), Expect = 2e-30
 Identities = 71/118 (60%), Positives = 82/118 (69%), Gaps = 18/118 (15%)
 Frame = +1

Query: 427 VWIANTRGNNYSRGHTSLAPSDPAYWDWSWDELVAYEF------------------GHSL 552
           VWI+NTRG  +S+GHTSL+P+DPAYW+WSWDEL AY+                   GHSL
Sbjct: 125 VWISNTRGTRHSQGHTSLSPNDPAYWNWSWDELAAYDLPATFNYVHEQTGQKLYYVGHSL 184

Query: 553 GTLIAFASLSQDKNLNMLHSAALLSLIAYLGQMSSFLARSAADIFLAEELKWPGLNEF 726
           GTLIA AS SQ+  LNML +AALLS IAYL Q+ S L R AAD+FLAEEL W GL EF
Sbjct: 185 GTLIALASFSQENLLNMLRAAALLSPIAYLNQIPSPLTRVAADLFLAEELYWLGLREF 242



 Score = 72.8 bits (177), Expect = 7e-11
 Identities = 34/54 (62%), Positives = 42/54 (77%)
 Frame = +3

Query: 120 SSVNE*SGICKLMDDTQGYLCEEHTVITEDGYILSMQRIPKGRSGRASDKPPVL 281
           SS N+  GIC  M  TQGY+C+EH V T+DGYILSMQR+P  RSG+ ++KPPVL
Sbjct: 41  SSPND-GGICMSMSATQGYICQEHKVTTQDGYILSMQRMPADRSGKPAEKPPVL 93


>ref|XP_002320493.1| predicted protein [Populus trichocarpa] gi|222861266|gb|EEE98808.1|
           predicted protein [Populus trichocarpa]
          Length = 409

 Score =  136 bits (343), Expect = 4e-30
 Identities = 71/118 (60%), Positives = 80/118 (67%), Gaps = 18/118 (15%)
 Frame = +1

Query: 427 VWIANTRGNNYSRGHTSLAPSDPAYWDWSWDELVAYEF------------------GHSL 552
           VWIAN+RG  +SRGH SL P+D  YWDW+WDEL AY+                   GHS 
Sbjct: 124 VWIANSRGTRFSRGHASLHPNDSVYWDWTWDELAAYDLPATFQYVHDQTGQNLHYVGHSQ 183

Query: 553 GTLIAFASLSQDKNLNMLHSAALLSLIAYLGQMSSFLARSAADIFLAEELKWPGLNEF 726
           GTLIAFA+ SQ+K LNML SA LLS IAYL QM S LAR AADIFLAE+L W GL+EF
Sbjct: 184 GTLIAFAAFSQEKLLNMLRSAVLLSPIAYLNQMPSPLARGAADIFLAEDLYWLGLHEF 241



 Score = 79.0 bits (193), Expect = 1e-12
 Identities = 38/69 (55%), Positives = 50/69 (72%)
 Frame = +3

Query: 75  FMPFGTQEVAVRTKLSSVNE*SGICKLMDDTQGYLCEEHTVITEDGYILSMQRIPKGRSG 254
           F  F +Q+    T +SS+N   G CK + + QGY C+EHTV T+DGYILS+QR+P G SG
Sbjct: 27  FYSFNSQD---GTSVSSLNANDGTCKSVVEPQGYACQEHTVTTKDGYILSLQRMPSGLSG 83

Query: 255 RASDKPPVL 281
           +A+DKPPVL
Sbjct: 84  QAADKPPVL 92


>emb|CBI28874.3| unnamed protein product [Vitis vinifera]
          Length = 402

 Score =  135 bits (341), Expect = 7e-30
 Identities = 71/121 (58%), Positives = 84/121 (69%), Gaps = 18/121 (14%)
 Frame = +1

Query: 427 VWIANTRGNNYSRGHTSLAPSDPAYWDWSWDELVAYEF------------------GHSL 552
           VWIANTRG  YSRGHTSL P D A+WDWSWDELV+Y+                   GHSL
Sbjct: 117 VWIANTRGTKYSRGHTSLDPGDSAFWDWSWDELVSYDLPASFQYVHDQTGQKLHYVGHSL 176

Query: 553 GTLIAFASLSQDKNLNMLHSAALLSLIAYLGQMSSFLARSAADIFLAEELKWPGLNEFNL 732
           GTLIA A+ SQ++ L+M  SA LLS IAY+GQM+S LAR+AAD F+AE L W GL+EF+ 
Sbjct: 177 GTLIALAAFSQNQLLSMSRSAVLLSPIAYVGQMTSPLARNAADNFIAESLYWLGLDEFDP 236

Query: 733 R 735
           R
Sbjct: 237 R 237



 Score = 73.2 bits (178), Expect = 5e-11
 Identities = 41/76 (53%), Positives = 51/76 (67%), Gaps = 6/76 (7%)
 Frame = +3

Query: 72  LFMPFGTQEVAVRTKLSS-----VNE*SGICKLMDDTQGYLCEEHTVITEDGYILSMQRI 236
           L + F      +RT+L S     ++   GICKLM +TQGY CEEH V T+DGYILS+QRI
Sbjct: 10  LVILFCRSAAGLRTRLFSNKDWVLDADEGICKLMVETQGYACEEHKVTTQDGYILSVQRI 69

Query: 237 PKGRSGRAS-DKPPVL 281
           P GRSG AS ++ PVL
Sbjct: 70  PVGRSGEASAERAPVL 85