BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00010139
         (931 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002301949.1| predicted protein [Populus trichocarpa] gi|2...   343   3e-92
ref|XP_003634352.1| PREDICTED: RNA pseudourine synthase 2, chlor...   342   6e-92
ref|XP_002519370.1| ribosomal pseudouridine synthase, putative [...   339   5e-91
ref|NP_177732.2| RNA pseudourine synthase 2 [Arabidopsis thalian...   331   1e-88
ref|NP_974151.1| RNA pseudourine synthase 2 [Arabidopsis thalian...   331   1e-88

>ref|XP_002301949.1| predicted protein [Populus trichocarpa] gi|222843675|gb|EEE81222.1|
           predicted protein [Populus trichocarpa]
          Length = 444

 Score =  343 bits (881), Expect = 3e-92
 Identities = 180/251 (71%), Positives = 202/251 (80%)
 Frame = +3

Query: 3   EESVDDVNSGKLRLDSWISSRITGISRARVQSSIKSGLVTVNGQKIDKVSHKVRYGDKVD 182
           EESVD   SGK RLDSWISSRI+GISRARVQSSIKSGLV+VNG+ IDKVSH V+ GDKV+
Sbjct: 84  EESVDS-KSGKSRLDSWISSRISGISRARVQSSIKSGLVSVNGKVIDKVSHNVKAGDKVN 142

Query: 183 CTISELQPLKAEPEDIPLDIVYEDNHVLVVNKPAHMVVHPAPGNATGTLVNGILHHCCLP 362
           C ISELQPL+AEPE+IPLDIVYED +VLVVNKPAHMVVHPAPGNATGTLVNGILHHC LP
Sbjct: 143 CVISELQPLRAEPENIPLDIVYEDENVLVVNKPAHMVVHPAPGNATGTLVNGILHHCSLP 202

Query: 363 ESSLMNLDIPXXXXXXXXXXXXXXXADDNYPEGLFSGMYEASIRPGIVHRLDKGTSGLLV 542
             S   +                    D+  EGL S  Y AS+RPGIVHRLDKGTSGLLV
Sbjct: 203 TVSSQEV------------LSGAEDVSDDDDEGLCSSSYAASVRPGIVHRLDKGTSGLLV 250

Query: 543 VAKDEHSHAHLSEQFKQHTIQRVYISLTCGVPSPTSGRVDIPIGRDLNNRIRMAAQTGSS 722
           VAKDEHSHAHLSEQFK HTI+RVY+SLTCGVP+P +GR+D+PIGRD++NRIRMAA    +
Sbjct: 251 VAKDEHSHAHLSEQFKLHTIKRVYVSLTCGVPAPMAGRIDVPIGRDVSNRIRMAAIVEPN 310

Query: 723 RCGKTRNAASR 755
           R G+ R+AASR
Sbjct: 311 R-GQARHAASR 320


>ref|XP_003634352.1| PREDICTED: RNA pseudourine synthase 2, chloroplastic-like [Vitis
           vinifera] gi|302141717|emb|CBI18920.3| unnamed protein
           product [Vitis vinifera]
          Length = 433

 Score =  342 bits (878), Expect = 6e-92
 Identities = 182/256 (71%), Positives = 202/256 (78%), Gaps = 5/256 (1%)
 Frame = +3

Query: 3   EESVDDVNS--GKLRLDSWISSRITGISRARVQSSIKSGLVTVNGQKIDKVSHKVRYGDK 176
           EE+V DVNS  GKLRLDSWISSRI GISRARVQSSI+SGLV+VN + +DKVSH +R GDK
Sbjct: 64  EETVVDVNSDSGKLRLDSWISSRIIGISRARVQSSIRSGLVSVNDRIVDKVSHVIRAGDK 123

Query: 177 VDCTISELQPLKAEPEDIPLDIVYEDNHVLVVNKPAHMVVHPAPGNATGTLVNGILHHCC 356
           V+CTISELQPL+AEPEDIPLDIVYED HVLVVNKPAHMVVHPAPGNA+GTLVNGILHHC 
Sbjct: 124 VNCTISELQPLRAEPEDIPLDIVYEDEHVLVVNKPAHMVVHPAPGNASGTLVNGILHHCS 183

Query: 357 LPESSLMNLDIPXXXXXXXXXXXXXXXADD---NYPEGLFSGMYEASIRPGIVHRLDKGT 527
           LP  +L N D                 +DD   ++  G   G  E +IRPGIVHRLDKGT
Sbjct: 184 LPTVALPNRD-----------SEEDDISDDEFNSFAAGQNCGTCETNIRPGIVHRLDKGT 232

Query: 528 SGLLVVAKDEHSHAHLSEQFKQHTIQRVYISLTCGVPSPTSGRVDIPIGRDLNNRIRMAA 707
           SGLLVVAKDEHSHAHLSEQFK H+I RVYISLT GVP+P SGRV+IP+GRD NNRIRM A
Sbjct: 233 SGLLVVAKDEHSHAHLSEQFKLHSIHRVYISLTSGVPTPLSGRVEIPVGRDPNNRIRMTA 292

Query: 708 QTGSSRCGKTRNAASR 755
             G S  G+ R+AASR
Sbjct: 293 VPGPSNSGQARHAASR 308


>ref|XP_002519370.1| ribosomal pseudouridine synthase, putative [Ricinus communis]
           gi|223541437|gb|EEF42987.1| ribosomal pseudouridine
           synthase, putative [Ricinus communis]
          Length = 399

 Score =  339 bits (870), Expect = 5e-91
 Identities = 180/257 (70%), Positives = 207/257 (80%), Gaps = 2/257 (0%)
 Frame = +3

Query: 3   EESVDDVN--SGKLRLDSWISSRITGISRARVQSSIKSGLVTVNGQKIDKVSHKVRYGDK 176
           EE+V  V+  S KLRLDSWISSRI+GISRARVQSSIKSGLV+VNG+ +DKVSH V++GDK
Sbjct: 59  EETVQVVHAKSVKLRLDSWISSRISGISRARVQSSIKSGLVSVNGRVVDKVSHNVKFGDK 118

Query: 177 VDCTISELQPLKAEPEDIPLDIVYEDNHVLVVNKPAHMVVHPAPGNATGTLVNGILHHCC 356
           V CTISELQPL+AEPED+PLDIVYED H+LVVNKP HMVVHPAPGN TGTLVNGILHHC 
Sbjct: 119 VTCTISELQPLRAEPEDMPLDIVYEDEHLLVVNKPPHMVVHPAPGNPTGTLVNGILHHCN 178

Query: 357 LPESSLMNLDIPXXXXXXXXXXXXXXXADDNYPEGLFSGMYEASIRPGIVHRLDKGTSGL 536
           LP  ++ + ++                +DD+  E  +S   E+SIRPGIVHRLDKGTSGL
Sbjct: 179 LPTVAISSQEV---------LSDAEDRSDDD--ERSYSSSCESSIRPGIVHRLDKGTSGL 227

Query: 537 LVVAKDEHSHAHLSEQFKQHTIQRVYISLTCGVPSPTSGRVDIPIGRDLNNRIRMAAQTG 716
           LVVAKDEH+HAHLSEQFKQHTIQRVYISLT GVPS  +GR+DIPIGRD+NNRIRM A  G
Sbjct: 228 LVVAKDEHAHAHLSEQFKQHTIQRVYISLTSGVPSALTGRIDIPIGRDVNNRIRMTAIPG 287

Query: 717 SSRCGKTRNAASR*FKV 767
             + G+ R+AASR FKV
Sbjct: 288 PIKQGRARHAASR-FKV 303


>ref|NP_177732.2| RNA pseudourine synthase 2 [Arabidopsis thaliana]
           gi|332197668|gb|AEE35789.1| RNA pseudourine synthase 2
           [Arabidopsis thaliana]
          Length = 313

 Score =  331 bits (849), Expect = 1e-88
 Identities = 173/259 (66%), Positives = 194/259 (74%), Gaps = 9/259 (3%)
 Frame = +3

Query: 6   ESVDDVNSGKLRLDSWISSRITGISRARVQSSIKSGLVTVNGQKIDKVSHKVRYGDKVDC 185
           E + D   GK+RLDSWISSRI G+SRARVQSSI+ GLVTVNG+ +DKVSH V+ GD+V+C
Sbjct: 72  EEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVSHNVKSGDEVNC 131

Query: 186 TISELQPLKAEPEDIPLDIVYEDNHVLVVNKPAHMVVHPAPGNATGTLVNGILHHCCLPE 365
           TISELQPLKAE EDIPLDIVYED HVLVVNKPAHMVVHPAPGN TGTLVNGILHHC LP 
Sbjct: 132 TISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNGILHHCSLPC 191

Query: 366 SSLMNLDIPXXXXXXXXXXXXXXXADDNYPEGLFS---------GMYEASIRPGIVHRLD 518
               N +                  DD+  E  FS           Y AS+RPGIVHRLD
Sbjct: 192 VDYSNSE-----------------EDDDSDEETFSDDEEMTTSPSSYAASVRPGIVHRLD 234

Query: 519 KGTSGLLVVAKDEHSHAHLSEQFKQHTIQRVYISLTCGVPSPTSGRVDIPIGRDLNNRIR 698
           KGT+GLLVVAKDEHSHAHL+EQFK HTI+RVY+SLT GVPSP  GR++IPIGRD +NRIR
Sbjct: 235 KGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIR 294

Query: 699 MAAQTGSSRCGKTRNAASR 755
           MAA  G  R G+ R+AASR
Sbjct: 295 MAAIPGGVRGGRARHAASR 313


>ref|NP_974151.1| RNA pseudourine synthase 2 [Arabidopsis thaliana]
           gi|122215273|sp|Q3ECD0.1|PUS2_ARATH RecName: Full=RNA
           pseudourine synthase 2, chloroplastic; AltName: Full=RNA
           pseudouridylate synthase 2; AltName: Full=RNA-uridine
           isomerase 2; Flags: Precursor
           gi|332197669|gb|AEE35790.1| RNA pseudourine synthase 2
           [Arabidopsis thaliana]
          Length = 430

 Score =  331 bits (849), Expect = 1e-88
 Identities = 173/259 (66%), Positives = 194/259 (74%), Gaps = 9/259 (3%)
 Frame = +3

Query: 6   ESVDDVNSGKLRLDSWISSRITGISRARVQSSIKSGLVTVNGQKIDKVSHKVRYGDKVDC 185
           E + D   GK+RLDSWISSRI G+SRARVQSSI+ GLVTVNG+ +DKVSH V+ GD+V+C
Sbjct: 72  EEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVSHNVKSGDEVNC 131

Query: 186 TISELQPLKAEPEDIPLDIVYEDNHVLVVNKPAHMVVHPAPGNATGTLVNGILHHCCLPE 365
           TISELQPLKAE EDIPLDIVYED HVLVVNKPAHMVVHPAPGN TGTLVNGILHHC LP 
Sbjct: 132 TISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNGILHHCSLPC 191

Query: 366 SSLMNLDIPXXXXXXXXXXXXXXXADDNYPEGLFS---------GMYEASIRPGIVHRLD 518
               N +                  DD+  E  FS           Y AS+RPGIVHRLD
Sbjct: 192 VDYSNSE-----------------EDDDSDEETFSDDEEMTTSPSSYAASVRPGIVHRLD 234

Query: 519 KGTSGLLVVAKDEHSHAHLSEQFKQHTIQRVYISLTCGVPSPTSGRVDIPIGRDLNNRIR 698
           KGT+GLLVVAKDEHSHAHL+EQFK HTI+RVY+SLT GVPSP  GR++IPIGRD +NRIR
Sbjct: 235 KGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIR 294

Query: 699 MAAQTGSSRCGKTRNAASR 755
           MAA  G  R G+ R+AASR
Sbjct: 295 MAAIPGGVRGGRARHAASR 313