BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00010036
         (1168 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002516770.1| vacuole membrane protein, putative [Ricinus ...   489   e-136
ref|XP_002281783.1| PREDICTED: vacuole membrane protein 1 [Vitis...   487   e-135
ref|XP_002312524.1| predicted protein [Populus trichocarpa] gi|2...   486   e-135
ref|NP_567450.1| SNARE associated Golgi protein [Arabidopsis tha...   482   e-134
gb|AAL24087.1| unknown protein [Arabidopsis thaliana]                 481   e-133

>ref|XP_002516770.1| vacuole membrane protein, putative [Ricinus communis]
            gi|223543858|gb|EEF45384.1| vacuole membrane protein,
            putative [Ricinus communis]
          Length = 427

 Score =  489 bits (1260), Expect = e-136
 Identities = 243/325 (74%), Positives = 269/325 (82%), Gaps = 1/325 (0%)
 Frame = +2

Query: 197  GLHQRHQRELENLTLTTQPFKTIKFFILAIIQYLKRIVLYLLGHGAWLTLLSIFLVLGGI 376
            GLH+R Q+ELENLTLTTQP KT+KFFILA+IQY K+ VLYLL  G WL LLSI +   GI
Sbjct: 21   GLHERQQQELENLTLTTQPLKTLKFFILAMIQYFKKSVLYLLAKGGWLMLLSIMVAAIGI 80

Query: 377  LLMNINGPHEKHIETIFLYLQFGLWWVALGVASSIGLGSGLHTFVLYLGPHIAFFTIKAM 556
            LL+ I+GPHEKHI+ +  Y +FGLWW+ALGVASSIGLGSGLHTFVLYLGPHIA FTIKAM
Sbjct: 81   LLVTIDGPHEKHIDELSHYSRFGLWWIALGVASSIGLGSGLHTFVLYLGPHIALFTIKAM 140

Query: 557  LCGRVDIKSAPYDTIQLKRVPSWLGRDCSEFGPPVFTASHGLRVPLSSILPQVQLEAILW 736
             CGRVD+KSA YDTIQLKR PSWL +DC EFG PVF  SHGLRVPLSSILPQVQ+EA+LW
Sbjct: 141  QCGRVDLKSAIYDTIQLKRGPSWLDKDCHEFGDPVFPLSHGLRVPLSSILPQVQIEAVLW 200

Query: 737  GFGTALGELPPYFISRAASLSGGK-XXXXXXXXXXXXXXGQMAMLLNRSKSWFLSHAQYL 913
            G GTALGELPPYFISRAAS+SG K               G +A  LN  K W LSH+Q+L
Sbjct: 201  GVGTALGELPPYFISRAASVSGSKVDAMEELNASSSEDSGFIATHLNAIKRWLLSHSQHL 260

Query: 914  NFFTILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKTHIQTVFIISVCNNQ 1093
            NFFTIL+LASVPNPLFDLAGIMCGQFGI FW+FF AT+IGKAIIKTHIQTVFIISVCNNQ
Sbjct: 261  NFFTILVLASVPNPLFDLAGIMCGQFGISFWKFFFATLIGKAIIKTHIQTVFIISVCNNQ 320

Query: 1094 LLYWIENELIWVLSHVPGFDSILPD 1168
            LL W+ENELIWVLS VPG  S+LP+
Sbjct: 321  LLNWMENELIWVLSFVPGLASVLPN 345


>ref|XP_002281783.1| PREDICTED: vacuole membrane protein 1 [Vitis vinifera]
            gi|297737027|emb|CBI26228.3| unnamed protein product
            [Vitis vinifera]
          Length = 439

 Score =  487 bits (1253), Expect = e-135
 Identities = 241/352 (68%), Positives = 280/352 (79%), Gaps = 1/352 (0%)
 Frame = +2

Query: 116  MGFSNEATTSQRSSLSDVSHGNGVTDSGLHQRHQRELENLTLTTQPFKTIKFFILAIIQY 295
            MG  NE  +   SS S  S    +  SGL +R Q+E+ENLTL T+PFK +KFFIL ++Q+
Sbjct: 1    MGSRNEVASP--SSASSSSGSLEMAISGLRERQQQEMENLTLKTEPFKVLKFFILVVVQH 58

Query: 296  LKRIVLYLLGHGAWLTLLSIFLVLGGILLMNINGPHEKHIETIFLYLQFGLWWVALGVAS 475
            +K+ V YLL  G W+ LLS+  +  G +LM I+GPHEKH++ +  Y  FGLWW+ALG+AS
Sbjct: 59   VKQSVAYLLAKGGWVMLLSVVAMALGFVLMAIDGPHEKHVQELHQYFLFGLWWIALGIAS 118

Query: 476  SIGLGSGLHTFVLYLGPHIAFFTIKAMLCGRVDIKSAPYDTIQLKRVPSWLGRDCSEFGP 655
            SIGLGSGLHTFVLYLGPHIA FTIKAM CGRVDIKSAPYDTI+LK  PSWL +DCSEFGP
Sbjct: 119  SIGLGSGLHTFVLYLGPHIALFTIKAMQCGRVDIKSAPYDTIRLKSGPSWLDKDCSEFGP 178

Query: 656  PVFTASHGLRVPLSSILPQVQLEAILWGFGTALGELPPYFISRAASLSGGK-XXXXXXXX 832
            PVF++ H LRVPLSSILPQVQ+EA+LWG GTALGELPPYFIS+AASLSG K         
Sbjct: 179  PVFSSLHALRVPLSSILPQVQVEAVLWGIGTALGELPPYFISKAASLSGSKSDAIEELDA 238

Query: 833  XXXXXXGQMAMLLNRSKSWFLSHAQYLNFFTILLLASVPNPLFDLAGIMCGQFGIPFWEF 1012
                  G ++  L + K WFLSH+Q+LNFFTIL+LASVPNPLFDLAGIMCGQFGIPFW+F
Sbjct: 239  SSPEDDGVVSTHLKQIKRWFLSHSQHLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWKF 298

Query: 1013 FLATMIGKAIIKTHIQTVFIISVCNNQLLYWIENELIWVLSHVPGFDSILPD 1168
            FLAT IGKAIIKTHIQTVFIISVCNNQLL WIENELIWVLS +PGF S+LP+
Sbjct: 299  FLATFIGKAIIKTHIQTVFIISVCNNQLLDWIENELIWVLSLIPGFASVLPN 350


>ref|XP_002312524.1| predicted protein [Populus trichocarpa] gi|222852344|gb|EEE89891.1|
            predicted protein [Populus trichocarpa]
          Length = 413

 Score =  486 bits (1251), Expect = e-135
 Identities = 236/324 (72%), Positives = 265/324 (81%), Gaps = 1/324 (0%)
 Frame = +2

Query: 200  LHQRHQRELENLTLTTQPFKTIKFFILAIIQYLKRIVLYLLGHGAWLTLLSIFLVLGGIL 379
            + +RHQ+ELENLTLT QPFKT+KFF LA++QY KR V YLL  G WL LLS  +   G++
Sbjct: 8    IRERHQQELENLTLTAQPFKTLKFFALAMVQYCKRSVFYLLAKGGWLMLLSTVVAAVGVV 67

Query: 380  LMNINGPHEKHIETIFLYLQFGLWWVALGVASSIGLGSGLHTFVLYLGPHIAFFTIKAML 559
            L+ I+ PHEKH+E +  YL+FGLWW+ALGVASSIGLGSGLHTFV+YLGPHIA FTIKAM 
Sbjct: 68   LVTIDSPHEKHVEELSHYLRFGLWWIALGVASSIGLGSGLHTFVMYLGPHIALFTIKAMQ 127

Query: 560  CGRVDIKSAPYDTIQLKRVPSWLGRDCSEFGPPVFTASHGLRVPLSSILPQVQLEAILWG 739
            CGRVD+KSAPYDTIQLKR PSWL RDC E+G PVF  SHGLRVPLSSILPQVQ+EAILWG
Sbjct: 128  CGRVDLKSAPYDTIQLKRGPSWLDRDCHEYGDPVFPLSHGLRVPLSSILPQVQVEAILWG 187

Query: 740  FGTALGELPPYFISRAASLSGGK-XXXXXXXXXXXXXXGQMAMLLNRSKSWFLSHAQYLN 916
             GTALGELPPYFISRAA +SG                 G +A  L   K W L+H+Q+LN
Sbjct: 188  VGTALGELPPYFISRAARMSGSNLDAMEELDASSDGDSGIVAARLKAIKHWLLNHSQHLN 247

Query: 917  FFTILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKTHIQTVFIISVCNNQL 1096
            FFTIL+LASVPNPLFDLAGIMCGQFG+PFW+FFLAT+IGKAI+KTHIQTVFIISVCNNQL
Sbjct: 248  FFTILVLASVPNPLFDLAGIMCGQFGVPFWKFFLATLIGKAIVKTHIQTVFIISVCNNQL 307

Query: 1097 LYWIENELIWVLSHVPGFDSILPD 1168
            L WIENELIWVL  VPGFDS LPD
Sbjct: 308  LNWIENELIWVLGLVPGFDSALPD 331


>ref|NP_567450.1| SNARE associated Golgi protein [Arabidopsis thaliana]
            gi|53749170|gb|AAU90070.1| At4g14950 [Arabidopsis
            thaliana] gi|332658128|gb|AEE83528.1| SNARE associated
            Golgi protein [Arabidopsis thaliana]
          Length = 416

 Score =  482 bits (1240), Expect = e-134
 Identities = 230/333 (69%), Positives = 270/333 (81%), Gaps = 1/333 (0%)
 Frame = +2

Query: 170  SHGNGVTDSGLHQRHQRELENLTLTTQPFKTIKFFILAIIQYLKRIVLYLLGHGAWLTLL 349
            S  + V  S L ++H++E+ENLTLTTQP  T+K F+ A IQY+KR + YLL HG W  L+
Sbjct: 8    SSSSDVAISALREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAHGGWFILI 67

Query: 350  SIFLVLGGILLMNINGPHEKHIETIFLYLQFGLWWVALGVASSIGLGSGLHTFVLYLGPH 529
            +  LV+ G LL+ ++GPH KH+E +  Y+++GLWW+ALGVASSIGLGSGLHTFVLYLGPH
Sbjct: 68   TTLLVVSGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTFVLYLGPH 127

Query: 530  IAFFTIKAMLCGRVDIKSAPYDTIQLKRVPSWLGRDCSEFGPPVFTASHGLRVPLSSILP 709
            IA FT+KA LCGRVD+KSAPYDTIQLKRVPSWL + CSEFGPP+  ++ G RVPL+SILP
Sbjct: 128  IALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRVPLTSILP 187

Query: 710  QVQLEAILWGFGTALGELPPYFISRAASLSGGK-XXXXXXXXXXXXXXGQMAMLLNRSKS 886
            QVQLEAILWG GTALGELPPYFISRAAS+SG                 G MA  LNR K 
Sbjct: 188  QVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMATHLNRVKR 247

Query: 887  WFLSHAQYLNFFTILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKTHIQTV 1066
            W L+H+Q+LNFFT+L+LASVPNPLFDLAGIMCGQFGIPFWEFFLAT+IGKAIIKTHIQT+
Sbjct: 248  WLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAIIKTHIQTI 307

Query: 1067 FIISVCNNQLLYWIENELIWVLSHVPGFDSILP 1165
            FII VCNNQLL W+ENELIW+LSHVPG  S+LP
Sbjct: 308  FIICVCNNQLLDWMENELIWILSHVPGLASMLP 340


>gb|AAL24087.1| unknown protein [Arabidopsis thaliana]
          Length = 416

 Score =  481 bits (1239), Expect = e-133
 Identities = 230/333 (69%), Positives = 270/333 (81%), Gaps = 1/333 (0%)
 Frame = +2

Query: 170  SHGNGVTDSGLHQRHQRELENLTLTTQPFKTIKFFILAIIQYLKRIVLYLLGHGAWLTLL 349
            S  + V  S L ++H++E+ENLTLTTQP  T+K F+ A IQY+KR + YLL HG W  L+
Sbjct: 8    SSSSDVAISALREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAHGGWFILI 67

Query: 350  SIFLVLGGILLMNINGPHEKHIETIFLYLQFGLWWVALGVASSIGLGSGLHTFVLYLGPH 529
            +  LV+ G LL+ ++GPH KH+E +  Y+++GLWW+ALGVASSIGLGSGLHTFVLYLGPH
Sbjct: 68   TTLLVVSGGLLVTVDGPHGKHVEEVLGYVRYGLWWIALGVASSIGLGSGLHTFVLYLGPH 127

Query: 530  IAFFTIKAMLCGRVDIKSAPYDTIQLKRVPSWLGRDCSEFGPPVFTASHGLRVPLSSILP 709
            IA FT+KA LCGRVD+KSAPYDTIQLKRVPSWL + CSEFGPP+  ++ G RVPL+SILP
Sbjct: 128  IALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRVPLTSILP 187

Query: 710  QVQLEAILWGFGTALGELPPYFISRAASLSGGK-XXXXXXXXXXXXXXGQMAMLLNRSKS 886
            QVQLEAILWG GTALGELPPYFISRAAS+SG                 G MA  LNR K 
Sbjct: 188  QVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMATHLNRVKR 247

Query: 887  WFLSHAQYLNFFTILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKTHIQTV 1066
            W L+H+Q+LNFFT+L+LASVPNPLFDLAGIMCGQFGIPFWEFFLAT+IGKAIIKTHIQT+
Sbjct: 248  WLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAIIKTHIQTI 307

Query: 1067 FIISVCNNQLLYWIENELIWVLSHVPGFDSILP 1165
            FII VCNNQLL W+ENELIW+LSHVPG  S+LP
Sbjct: 308  FIICVCNNQLLDWMENELIWILSHVPGLASMLP 340