BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00009663
         (622 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADK91077.1| delta-8 sphingolipid desaturase [Helianthus annuus]    358   4e-97
sp|Q43469.1|SLD1_HELAN RecName: Full=Delta(8)-fatty-acid desatur...   356   2e-96
gb|ABA55805.1| delta-8 sphingolipid desaturase [Crepis alpina]        356   2e-96
ref|XP_002876622.1| delta-8 sphingolipid desaturase [Arabidopsis...   311   8e-83
ref|XP_002279227.1| PREDICTED: fatty acid desaturase 3 [Vitis vi...   310   2e-82

>gb|ADK91077.1| delta-8 sphingolipid desaturase [Helianthus annuus]
          Length = 466

 Score =  358 bits (919), Expect = 4e-97
 Identities = 166/206 (80%), Positives = 181/206 (87%)
 Frame = -3

Query: 620 VIYFLCFVALMFSGCVYGVLKSDSFGVHMLSAALLGLCWIQMAYLGHDSGHYQAMATRRS 441
           VIY LCFV+L+ SGCVYGVL S SF VHML+ ALLGL WIQ+AYLGHD+GHYQ M+TR  
Sbjct: 131 VIYSLCFVSLLLSGCVYGVLFSSSFWVHMLAGALLGLAWIQIAYLGHDAGHYQMMSTRGW 190

Query: 440 NKIAGILIGNCVTGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSDTLFRSITSKF 261
           NK AGI IGNC+TGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVS  LF S+TS F
Sbjct: 191 NKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSRLFNSLTSVF 250

Query: 260 YERKLTFDPIARFFISYQHVTYYPVMCVARVNLYLQTLLLLCSKRKIADRKLNIIGTLVF 81
           Y R+LTFD +ARFF+SYQH +YYP+MCVARVNLYLQTLLLL SKRK+ D+ LNI GTLVF
Sbjct: 251 YGRQLTFDRLARFFVSYQHYSYYPIMCVARVNLYLQTLLLLLSKRKVPDKGLNIFGTLVF 310

Query: 80  WTWFPLLVSCLPNWTERVMFVLVSFC 3
           WTWFPLLVSCLPNW ERV FVLVSFC
Sbjct: 311 WTWFPLLVSCLPNWPERVAFVLVSFC 336


>sp|Q43469.1|SLD1_HELAN RecName: Full=Delta(8)-fatty-acid desaturase; AltName:
           Full=Delta(8)-sphingolipid desaturase
           gi|1040729|emb|CAA60621.1| delta-8 sphingolipid
           desaturase [Helianthus annuus]
          Length = 458

 Score =  356 bits (913), Expect = 2e-96
 Identities = 165/206 (80%), Positives = 180/206 (87%)
 Frame = -3

Query: 620 VIYFLCFVALMFSGCVYGVLKSDSFGVHMLSAALLGLCWIQMAYLGHDSGHYQAMATRRS 441
           VIY LCFV+L+ S CVYGVL S SF +HMLS A+LGL W+Q+AYLGHD+GHYQ MATR  
Sbjct: 123 VIYSLCFVSLLLSACVYGVLYSGSFWIHMLSGAILGLAWMQIAYLGHDAGHYQMMATRGW 182

Query: 440 NKIAGILIGNCVTGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSDTLFRSITSKF 261
           NK AGI IGNC+TGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVS  LF SITS F
Sbjct: 183 NKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNSITSVF 242

Query: 260 YERKLTFDPIARFFISYQHVTYYPVMCVARVNLYLQTLLLLCSKRKIADRKLNIIGTLVF 81
           Y R+LTFDP+ARFF+SYQH  YYP+MCVARVNLYLQT+LLL SKRKI DR LNI+GTL+F
Sbjct: 243 YGRQLTFDPLARFFVSYQHYLYYPIMCVARVNLYLQTILLLISKRKIPDRGLNILGTLIF 302

Query: 80  WTWFPLLVSCLPNWTERVMFVLVSFC 3
           WTWFPLLVS LPNW ERV FVLVSFC
Sbjct: 303 WTWFPLLVSRLPNWPERVAFVLVSFC 328


>gb|ABA55805.1| delta-8 sphingolipid desaturase [Crepis alpina]
          Length = 469

 Score =  356 bits (913), Expect = 2e-96
 Identities = 165/206 (80%), Positives = 179/206 (86%)
 Frame = -3

Query: 620 VIYFLCFVALMFSGCVYGVLKSDSFGVHMLSAALLGLCWIQMAYLGHDSGHYQAMATRRS 441
           VIY LCFV+++ S CVYGVL S S  VHM + ALLGL W+Q+AYLGHD+GHYQ MATR  
Sbjct: 134 VIYSLCFVSILLSLCVYGVLCSASVTVHMFAGALLGLAWMQIAYLGHDAGHYQMMATRGW 193

Query: 440 NKIAGILIGNCVTGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSDTLFRSITSKF 261
           NK AGI IGNC+TGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVS  LF SITS F
Sbjct: 194 NKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFESITSVF 253

Query: 260 YERKLTFDPIARFFISYQHVTYYPVMCVARVNLYLQTLLLLCSKRKIADRKLNIIGTLVF 81
           Y R+LTFD +ARFFISYQH TYYP+MCV RVNLYLQT+LLLCSKR+I DR LNIIGTL+F
Sbjct: 254 YGRRLTFDSVARFFISYQHYTYYPIMCVTRVNLYLQTILLLCSKRRIPDRTLNIIGTLIF 313

Query: 80  WTWFPLLVSCLPNWTERVMFVLVSFC 3
           WTWFPLLVSCLPNW ERV FVLVSFC
Sbjct: 314 WTWFPLLVSCLPNWPERVAFVLVSFC 339


>ref|XP_002876622.1| delta-8 sphingolipid desaturase [Arabidopsis lyrata subsp. lyrata]
           gi|297322460|gb|EFH52881.1| delta-8 sphingolipid
           desaturase [Arabidopsis lyrata subsp. lyrata]
          Length = 449

 Score =  311 bits (796), Expect = 8e-83
 Identities = 139/204 (68%), Positives = 161/204 (78%)
 Frame = -3

Query: 617 IYFLCFVALMFSGCVYGVLKSDSFGVHMLSAALLGLCWIQMAYLGHDSGHYQAMATRRSN 438
           +Y L FVA MF G +YGVL   S   H ++AALLGL WIQ AY+GHDSGHY  M+ +  N
Sbjct: 115 LYTLAFVAAMFVGVLYGVLACTSVFAHQIAAALLGLLWIQSAYIGHDSGHYVVMSNKSYN 174

Query: 437 KIAGILIGNCVTGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSDTLFRSITSKFY 258
           + A +L GNC+TGISIAWWKWTHNAHH+ACNSLDYDPDLQH+P+ AVS   F S+TS+FY
Sbjct: 175 RFAQLLSGNCLTGISIAWWKWTHNAHHLACNSLDYDPDLQHIPVFAVSTKFFNSLTSRFY 234

Query: 257 ERKLTFDPIARFFISYQHVTYYPVMCVARVNLYLQTLLLLCSKRKIADRKLNIIGTLVFW 78
           +RKLTFDP+ARF ISYQH TYYPVMC  R+NL++QT +LL SKRK+ DR LN  G LVFW
Sbjct: 235 DRKLTFDPVARFLISYQHFTYYPVMCFGRINLFIQTFILLFSKRKVPDRALNFAGILVFW 294

Query: 77  TWFPLLVSCLPNWTERVMFVLVSF 6
           TWFPLLVSCLPNW ER  FV  SF
Sbjct: 295 TWFPLLVSCLPNWPERFFFVFTSF 318


>ref|XP_002279227.1| PREDICTED: fatty acid desaturase 3 [Vitis vinifera]
          Length = 447

 Score =  310 bits (793), Expect = 2e-82
 Identities = 143/204 (70%), Positives = 165/204 (80%)
 Frame = -3

Query: 620 VIYFLCFVALMFSGCVYGVLKSDSFGVHMLSAALLGLCWIQMAYLGHDSGHYQAMATRRS 441
           ++Y    +  +F  C+YGVL  DSF VH+  AALLG  WIQ +YLGHDSGHYQ M++   
Sbjct: 112 ILYTCSIIGFLFMVCIYGVLCVDSFWVHLGCAALLGFLWIQSSYLGHDSGHYQIMSSPGF 171

Query: 440 NKIAGILIGNCVTGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSDTLFRSITSKF 261
           NK+A +LIGNC+TGISIAWWKWTHNAHHIACNSLDYDPDLQH+P+ AVS +LF SI S F
Sbjct: 172 NKLAQMLIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHIPVFAVSSSLFDSINSVF 231

Query: 260 YERKLTFDPIARFFISYQHVTYYPVMCVARVNLYLQTLLLLCSKRKIADRKLNIIGTLVF 81
           Y R L FD +ARFF+SYQH TYYPVMCVAR+NL+LQTLLLL S R I DR LNI+G  VF
Sbjct: 232 YGRILKFDSVARFFVSYQHWTYYPVMCVARINLFLQTLLLLFSGRNIPDRALNIMGICVF 291

Query: 80  WTWFPLLVSCLPNWTERVMFVLVS 9
           WTW+PLLVSCLPNW ERVMFV+VS
Sbjct: 292 WTWYPLLVSCLPNWGERVMFVIVS 315