BLASTX nr result
ID: Bupleurum21_contig00008783
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00008783
(2502 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002267958.1| PREDICTED: 3-epi-6-deoxocathasterone 23-mono... 594 e-167
ref|XP_002310278.1| predicted protein [Populus trichocarpa] gi|2... 590 e-166
ref|XP_003538868.1| PREDICTED: 3-epi-6-deoxocathasterone 23-mono... 586 e-164
ref|NP_568002.1| 3-epi-6-deoxocathasterone 23-monooxygenase [Ara... 585 e-164
dbj|BAA37167.1| cytochrome P450 [Arabidopsis thaliana] 585 e-164
>ref|XP_002267958.1| PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Vitis
vinifera] gi|297735073|emb|CBI17435.3| unnamed protein
product [Vitis vinifera]
Length = 486
Score = 594 bits (1531), Expect = e-167
Identities = 291/461 (63%), Positives = 371/461 (80%), Gaps = 2/461 (0%)
Frame = -2
Query: 1760 EKSCKIPRGGSGWPVIGETLEFIASGYSSRPVAFMDKRKSQYGKVFRTHILGRPIIVSTD 1581
E+ +PRG GWPVIGETL+FIASG+SSRPV+FM+KRKS+YGKVF+T+ILG+P+IVSTD
Sbjct: 29 EEGSGVPRGNPGWPVIGETLDFIASGWSSRPVSFMEKRKSRYGKVFKTNILGKPVIVSTD 88
Query: 1580 TEVNKAILQNHGNVFIPSYPKSVTELLGKSSILQTNGPLHKKLHSLIGGFLRSPQLKSRI 1401
EVNK +LQN GNVFIP+YP+++TE+LG+SSILQ NG L K++H+L+GGFLRSPQLK+RI
Sbjct: 89 PEVNKVVLQNIGNVFIPAYPQTITEILGESSILQMNGSLQKRVHALLGGFLRSPQLKARI 148
Query: 1400 AIEIEDSIKHSLSVWEHKEQPIL-LQYETKQISFEVLVRVLMSVGPGEDMEFLRREFQEF 1224
+IE +K +L W K+Q I+ +Q E ++I+F+VLVRVL+S+ PGED+ F++REF E
Sbjct: 149 TRDIERYVKLTLDSW--KDQHIVYVQDEVRKITFDVLVRVLVSITPGEDLNFMKREFAEV 206
Query: 1223 IKGLICIPVKFPGTRLYKSLQAKKNLFKMVRKIVEERKLGMEKTGQK-ISGSPSDVIEVL 1047
IKGLIC+P+K PGTRLYKSL+AK+ L K+VR+IVEERK M++ + + G P+D ++VL
Sbjct: 207 IKGLICLPIKLPGTRLYKSLKAKERLLKLVRRIVEERKAAMDRGDHETLKGPPNDAVDVL 266
Query: 1046 LNXXXXXXXXXXXXXXXXXXXXXDNIIEMLIAGEDTVPMVMTLAIKYLSDNPVALSRVVE 867
L N+IEM+I GE+T+P MTLA+K+LSD PVAL++++E
Sbjct: 267 LRDTGDSSETSRLSLDFISS----NMIEMMIPGEETLPTAMTLAVKFLSDCPVALNQLME 322
Query: 866 ENMNLKRLKTESCEGHAWTDYMSLQFTQNVISETLRMANIINAVWRKSVKDVEIKGYLIP 687
ENM LK+ K S E +AWTDYM L FTQNVI+ETLRMANIINAVWRK+VKDV+IK YLIP
Sbjct: 323 ENMELKKGKALSGEDYAWTDYMCLPFTQNVINETLRMANIINAVWRKAVKDVKIKDYLIP 382
Query: 686 KDWCVVASFTSVHMDEENYENPYQFNPWRWETRGIVNASNAFTPFSGGQRLCPGLELSRL 507
+ W V+ASFTS+HMDEENYENPYQFNPWRWE VN +N++TPF GGQRLCPGLELS+L
Sbjct: 383 EGWGVMASFTSIHMDEENYENPYQFNPWRWEKAASVNNNNSYTPFGGGQRLCPGLELSKL 442
Query: 506 EISIFLHHFVTKYRWEAEDDNIVTFPTVKMKRKLPITIMPM 384
EISIFLHH VT YRW A+ D++V FPTVKM++KLPIT+ P+
Sbjct: 443 EISIFLHHLVTTYRWVAKKDDVVYFPTVKMRKKLPITVTPL 483
>ref|XP_002310278.1| predicted protein [Populus trichocarpa] gi|222853181|gb|EEE90728.1|
predicted protein [Populus trichocarpa]
Length = 463
Score = 590 bits (1522), Expect = e-166
Identities = 288/460 (62%), Positives = 368/460 (80%), Gaps = 3/460 (0%)
Frame = -2
Query: 1763 DEKSCKIPRGGSGWPVIGETLEFIASGYSSRPVAFMDKRKSQYGKVFRTHILGRPIIVST 1584
++KS +P+G GWP+IGETL+FIA+GY+S+PV+FM+KR+S YGKVF+THILG PIIVST
Sbjct: 2 EQKSGVLPKGSFGWPLIGETLDFIAAGYTSQPVSFMEKRRSLYGKVFKTHILGTPIIVST 61
Query: 1583 DTEVNKAILQNHGNVFIPSYPKSVTELLGKSSILQTNGPLHKKLHSLIGGFLRSPQLKSR 1404
D VNK +LQNHGN+FIP+YPKSV E+ G+ SILQTNG L KK+H+LIGGFLRSPQ K+R
Sbjct: 62 DPAVNKVVLQNHGNIFIPAYPKSVREIFGEYSILQTNGTLQKKVHALIGGFLRSPQFKTR 121
Query: 1403 IAIEIEDSIKHSLSVWEHKEQPIL-LQYETKQISFEVLVRVLMSVGPGEDMEFLRREFQE 1227
I +IE +K +L+ W K+ P+L +Q ET++ +F+VLV+ L+S+ P +D++FL+REF E
Sbjct: 122 ITKDIEHHVKLTLTSW--KDLPLLFVQEETQKFTFKVLVKALLSLDPSDDLDFLKREFDE 179
Query: 1226 FIKGLICIPVKFPGTRLYKSLQAKKNLFKMVRKIVEERKLGMEKTGQKISGS--PSDVIE 1053
FIKGLIC+P+K PGTRLYKSL+AK+ +FK+V+KIVE+RKLG+EK+ + + P+D ++
Sbjct: 180 FIKGLICLPIKLPGTRLYKSLKAKERIFKLVKKIVEQRKLGLEKSEDQEHQNTIPNDAVD 239
Query: 1052 VLLNXXXXXXXXXXXXXXXXXXXXXDNIIEMLIAGEDTVPMVMTLAIKYLSDNPVALSRV 873
VLL +IIE++I GE+TVPM MTLA+K+LSD PVAL +
Sbjct: 240 VLLRDISGEQNDKQSLSLDFIIS---HIIELMIPGEETVPMSMTLAVKFLSDCPVALELL 296
Query: 872 VEENMNLKRLKTESCEGHAWTDYMSLQFTQNVISETLRMANIINAVWRKSVKDVEIKGYL 693
EENM LKR KT+S + + WTDYMSLQFTQNVI+ETLR+ANIINAVWRK++KDVEIKG L
Sbjct: 297 TEENMKLKRQKTDSGDDYCWTDYMSLQFTQNVINETLRVANIINAVWRKALKDVEIKGIL 356
Query: 692 IPKDWCVVASFTSVHMDEENYENPYQFNPWRWETRGIVNASNAFTPFSGGQRLCPGLELS 513
IP+ WCV+ SF+SVHMDEENYENP++F+PWRWE G +N FTPF GGQRLCPGLELS
Sbjct: 357 IPEGWCVLTSFSSVHMDEENYENPHEFDPWRWEKTGASVKNNCFTPFGGGQRLCPGLELS 416
Query: 512 RLEISIFLHHFVTKYRWEAEDDNIVTFPTVKMKRKLPITI 393
RLEISIFLHH VT YRW AE D+I+ FPTVK+K+KLPI +
Sbjct: 417 RLEISIFLHHLVTTYRWIAEKDDIIYFPTVKLKKKLPIRV 456
>ref|XP_003538868.1| PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase-like [Glycine
max]
Length = 483
Score = 586 bits (1510), Expect = e-164
Identities = 285/469 (60%), Positives = 361/469 (76%), Gaps = 8/469 (1%)
Frame = -2
Query: 1766 EDEKSC---KIPRGGSGWPVIGETLEFIASGYSSRPVAFMDKRKSQYGKVFRTHILGRPI 1596
+DEK+ K+P+G SGWP++GETL+FIASGY+S PV+F++KRKS YG VF+T ILG +
Sbjct: 18 DDEKTVAKGKVPKGNSGWPLLGETLDFIASGYTSTPVSFLEKRKSLYGNVFKTCILGSNV 77
Query: 1595 IVSTDTEVNKAILQNHGNVFIPSYPKSVTELLGKSSILQTNGPLHKKLHSLIGGFLRSPQ 1416
IVSTD +VNK +LQN N F+P+YPKS+ EL+G+ SIL+ NG +HKK+H+LI GFLRSPQ
Sbjct: 78 IVSTDPDVNKVVLQNQANNFVPAYPKSIRELMGEQSILKMNGTMHKKVHTLIAGFLRSPQ 137
Query: 1415 LKSRIAIEIEDSIKHSLSVWEHKEQPILLQYETKQISFEVLVRVLMSVGPGEDMEFLRRE 1236
LK+RI +IE ++K + W QPI +Q + K+I+F VL++VLMSVGPGED++FL RE
Sbjct: 138 LKARITRDIEHTVKQCFASWT-PHQPIYVQDQVKKITFPVLIKVLMSVGPGEDLDFLYRE 196
Query: 1235 FQEFIKGLICIPVKFPGTRLYKSLQAKKNLFKMVRKIVEERKL-----GMEKTGQKISGS 1071
F EFIKGLIC+P+KFPGTRLYKSL+AK + KMVR IVEERK + G ++ +
Sbjct: 197 FAEFIKGLICLPLKFPGTRLYKSLKAKDRMVKMVRNIVEERKKLQKDNNADDHGDTVAVA 256
Query: 1070 PSDVIEVLLNXXXXXXXXXXXXXXXXXXXXXDNIIEMLIAGEDTVPMVMTLAIKYLSDNP 891
+DV++VLL NIIEM++ GE+T+P MT+A+K+LSD+P
Sbjct: 257 VNDVVDVLLRDKVDSNSSSRLTPEMISQ----NIIEMMVPGEETLPTAMTIALKFLSDSP 312
Query: 890 VALSRVVEENMNLKRLKTESCEGHAWTDYMSLQFTQNVISETLRMANIINAVWRKSVKDV 711
+ALS++ EENM LKRLKT + +AWTDYMSL FTQNVISETLRMANI+N +WRKSV D+
Sbjct: 313 LALSKLQEENMELKRLKTNCSDDYAWTDYMSLPFTQNVISETLRMANIVNGIWRKSVNDI 372
Query: 710 EIKGYLIPKDWCVVASFTSVHMDEENYENPYQFNPWRWETRGIVNASNAFTPFSGGQRLC 531
EIKGYLIPK WCV+AS TSVHMD +NYENP++F+PWRWE G+V +N FTPF GG RLC
Sbjct: 373 EIKGYLIPKHWCVMASLTSVHMDGKNYENPFKFDPWRWEKIGVVAGNNCFTPFGGGHRLC 432
Query: 530 PGLELSRLEISIFLHHFVTKYRWEAEDDNIVTFPTVKMKRKLPITIMPM 384
PGLELSRLE+SIFLHH VT YRW AE D I+ FPTVKMKRKLPI++ P+
Sbjct: 433 PGLELSRLELSIFLHHLVTTYRWVAERDEIIYFPTVKMKRKLPISVQPI 481
>ref|NP_568002.1| 3-epi-6-deoxocathasterone 23-monooxygenase [Arabidopsis thaliana]
gi|150421525|sp|Q9M066.3|C90C1_ARATH RecName:
Full=3-epi-6-deoxocathasterone 23-monooxygenase; AltName:
Full=Cytochrome P450 90C1; AltName: Full=Protein
ROTUNDIFOLIA 3 gi|115646893|gb|ABJ17155.1| At4g36380
[Arabidopsis thaliana] gi|332661249|gb|AEE86649.1|
3-epi-6-deoxocathasterone 23-monooxygenase [Arabidopsis
thaliana]
Length = 524
Score = 585 bits (1509), Expect = e-164
Identities = 291/460 (63%), Positives = 356/460 (77%), Gaps = 2/460 (0%)
Frame = -2
Query: 1766 EDEKSCKIPRGGSGWPVIGETLEFIASGYSSRPVAFMDKRKSQYGKVFRTHILGRPIIVS 1587
E++K IP G GWPVIGETL FIA GYSSRPV FMDKRKS YGKVF+T+I+G PII+S
Sbjct: 62 EEKKKGMIPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIIS 121
Query: 1586 TDTEVNKAILQNHGNVFIPSYPKSVTELLGKSSILQTNGPLHKKLHSLIGGFLRSPQLKS 1407
TD EVNK +LQNHGN F+P+YPKS+TELLG++SIL NGP K+LH+LIG FLRSP LK
Sbjct: 122 TDAEVNKVVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKD 181
Query: 1406 RIAIEIEDSIKHSLSVWEHKEQPIL-LQYETKQISFEVLVRVLMSVGPGEDMEFLRREFQ 1230
RI +IE S+ +L+ W + P++ +Q E K+++FE+LV+VLMS PGEDM L+ EF+
Sbjct: 182 RITRDIEASVVLTLASWA--QLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFE 239
Query: 1229 EFIKGLICIPVKFPGTRLYKSLQAKKNLFKMVRKIVEERKLGMEKTGQKISGSPSDVIEV 1050
EFIKGLICIP+KFPGTRLYKSL+AK+ L KMV+K+VEER++ M T +DV++V
Sbjct: 240 EFIKGLICIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPA-----NDVVDV 294
Query: 1049 LLNXXXXXXXXXXXXXXXXXXXXXDNIIEMLIAGEDTVPMVMTLAIKYLSDNPVALSRVV 870
LL I+EM+I GE+T+P MTLA+K+LSDNPVAL+++V
Sbjct: 295 LLRDGGDSEKQSQPSDFVSG-----KIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLV 349
Query: 869 EENMNLKRLKTESCEGHAWTDYMSLQFTQNVISETLRMANIINAVWRKSVKDVEIKGYLI 690
EENM +KR K E E + WTDYMSL FTQNVI+ETLRMANIIN VWRK++KDVEIKGYLI
Sbjct: 350 EENMEMKRRKLELGEEYKWTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLI 409
Query: 689 PKDWCVVASFTSVHMDEENYENPYQFNPWRWE-TRGIVNASNAFTPFSGGQRLCPGLELS 513
PK WCV+ASF SVHMDE+ Y+NPYQF+PWRW+ G N+S FTPF GGQRLCPGLELS
Sbjct: 410 PKGWCVLASFISVHMDEDIYDNPYQFDPWRWDRINGSANSSICFTPFGGGQRLCPGLELS 469
Query: 512 RLEISIFLHHFVTKYRWEAEDDNIVTFPTVKMKRKLPITI 393
+LEISIFLHH VT+Y W AE+D IV+FPTVKMKR+LPI +
Sbjct: 470 KLEISIFLHHLVTRYSWTAEEDEIVSFPTVKMKRRLPIRV 509
>dbj|BAA37167.1| cytochrome P450 [Arabidopsis thaliana]
Length = 524
Score = 585 bits (1509), Expect = e-164
Identities = 291/460 (63%), Positives = 356/460 (77%), Gaps = 2/460 (0%)
Frame = -2
Query: 1766 EDEKSCKIPRGGSGWPVIGETLEFIASGYSSRPVAFMDKRKSQYGKVFRTHILGRPIIVS 1587
E++K IP G GWPVIGETL FIA GYSSRPV FMDKRKS YGKVF+T+I+G PII+S
Sbjct: 62 EEKKKGMIPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIIS 121
Query: 1586 TDTEVNKAILQNHGNVFIPSYPKSVTELLGKSSILQTNGPLHKKLHSLIGGFLRSPQLKS 1407
TD EVNK +LQNHGN F+P+YPKS+TELLG++SIL NGP K+LH+LIG FLRSP LK
Sbjct: 122 TDAEVNKVVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKD 181
Query: 1406 RIAIEIEDSIKHSLSVWEHKEQPIL-LQYETKQISFEVLVRVLMSVGPGEDMEFLRREFQ 1230
RI +IE S+ +L+ W + P++ +Q E K+++FE+LV+VLMS PGEDM L+ EF+
Sbjct: 182 RITRDIEASVVLTLASWA--QLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFE 239
Query: 1229 EFIKGLICIPVKFPGTRLYKSLQAKKNLFKMVRKIVEERKLGMEKTGQKISGSPSDVIEV 1050
EFIKGLICIP+KFPGTRLYKSL+AK+ L KMV+K+VEER++ M T +DV++V
Sbjct: 240 EFIKGLICIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPA-----NDVVDV 294
Query: 1049 LLNXXXXXXXXXXXXXXXXXXXXXDNIIEMLIAGEDTVPMVMTLAIKYLSDNPVALSRVV 870
LL I+EM+I GE+T+P MTLA+K+LSDNPVAL+++V
Sbjct: 295 LLRDGGDSEKQSQPSDFVSG-----KIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLV 349
Query: 869 EENMNLKRLKTESCEGHAWTDYMSLQFTQNVISETLRMANIINAVWRKSVKDVEIKGYLI 690
EENM +KR K E E + WTDYMSL FTQNVI+ETLRMANIIN VWRK++KDVEIKGYLI
Sbjct: 350 EENMEMKRRKLELGEEYKWTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLI 409
Query: 689 PKDWCVVASFTSVHMDEENYENPYQFNPWRWE-TRGIVNASNAFTPFSGGQRLCPGLELS 513
PK WCV+ASF SVHMDE+ Y+NPYQF+PWRW+ G N+S FTPF GGQRLCPGLELS
Sbjct: 410 PKGWCVLASFISVHMDEDIYDNPYQFDPWRWDRINGSANSSICFTPFGGGQRLCPGLELS 469
Query: 512 RLEISIFLHHFVTKYRWEAEDDNIVTFPTVKMKRKLPITI 393
+LEISIFLHH VT+Y W AE+D IV+FPTVKMKR+LPI +
Sbjct: 470 KLEISIFLHHLVTRYSWTAEEDEIVSFPTVKMKRRLPIRV 509