BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00008615
         (1181 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002281763.2| PREDICTED: uncharacterized protein LOC100254...   424   e-116
ref|XP_004149889.1| PREDICTED: uncharacterized protein LOC101220...   421   e-115
ref|XP_003609239.1| E3 ubiquitin-protein ligase MARCH3 [Medicago...   412   e-113
gb|AFK48121.1| unknown [Lotus japonicus]                              408   e-111
ref|NP_001241911.1| uncharacterized protein LOC100818950 [Glycin...   408   e-111

>ref|XP_002281763.2| PREDICTED: uncharacterized protein LOC100254401 [Vitis vinifera]
          Length = 304

 Score =  424 bits (1089), Expect = e-116
 Identities = 198/297 (66%), Positives = 218/297 (73%), Gaps = 2/297 (0%)
 Frame = +2

Query: 86  MNGELQLETLADRNPSDLDPLIDNH--NRGXXXXXXXXXXXXXGSVPCCRICLESGDNDD 259
           M GE+QLE   +RNP D DPL++N   +               GSVPCCRICLE     D
Sbjct: 1   MKGEVQLEPSGERNPRDSDPLLENQADSSTGSSSEINSEDIEAGSVPCCRICLECDGEPD 60

Query: 260 DDLISPCMCKGTQQFVHRMCLDHWRSVKEGFAFSHCTTCKAQFHLEVVKLEDSSWRKMKF 439
           D+LISPCMCKGTQQFVHR CLDHWRSVKEGFAFSHCTTCKAQ+HL+V   ED+SWRK+KF
Sbjct: 61  DELISPCMCKGTQQFVHRSCLDHWRSVKEGFAFSHCTTCKAQYHLQVALFEDNSWRKIKF 120

Query: 440 RLFVARDIFLVFLAVQTVIAAIAGLAYLLDKDGAFRNSFDDSWDRILSKHPIPFYYCXXX 619
           RLFVARD+FLVFLAVQTVI A+ G AY++DKDGAFRNSF D WDRILS+HPIPFYYC   
Sbjct: 121 RLFVARDVFLVFLAVQTVIGAMGGFAYIMDKDGAFRNSFSDGWDRILSRHPIPFYYCIGV 180

Query: 620 XXXXXXXXXXXXXXHCSAFNGSDPRMAACQNCCYGWGILDCFPASMEACXXXXXXXXXXX 799
                         HCS+ N +DP MA CQNCCYGWGILDCFPASMEAC           
Sbjct: 181 LAFFVLLGFFGLILHCSSLNSNDPHMAGCQNCCYGWGILDCFPASMEACFALVIVFVVIF 240

Query: 800 XXXXXXYAFLAATMAIQRIWQRHYHILTKRELTQEYVVKDLHGCYTPPKLDAEHEER 970
                 Y FLAATMAIQRIWQRHYHILTKRELT+EY+V+DL GCYTPPKLD EHEER
Sbjct: 241 AILGVAYGFLAATMAIQRIWQRHYHILTKRELTKEYIVEDLRGCYTPPKLDPEHEER 297


>ref|XP_004149889.1| PREDICTED: uncharacterized protein LOC101220313 [Cucumis sativus]
           gi|449533550|ref|XP_004173737.1| PREDICTED:
           uncharacterized LOC101220313 [Cucumis sativus]
          Length = 305

 Score =  421 bits (1083), Expect = e-115
 Identities = 197/297 (66%), Positives = 220/297 (74%), Gaps = 3/297 (1%)
 Frame = +2

Query: 86  MNGELQLETLADRNPSDLDPLIDNHNRGXXXXXXXXXXXXX---GSVPCCRICLESGDND 256
           M GE+QLE+   +NPSD DPL++N N                  GS+PCCRICLES    
Sbjct: 1   MKGEVQLESSIGQNPSDSDPLLENQNGSSSHGTSDEIKDEDTEAGSIPCCRICLESDAEP 60

Query: 257 DDDLISPCMCKGTQQFVHRMCLDHWRSVKEGFAFSHCTTCKAQFHLEVVKLEDSSWRKMK 436
           +D+LISPCMCKGTQQFVHR CLDHWRSVKEGFAFSHCTTCKAQFHL+V   ED+SWRK+K
Sbjct: 61  EDELISPCMCKGTQQFVHRSCLDHWRSVKEGFAFSHCTTCKAQFHLKVSLFEDNSWRKVK 120

Query: 437 FRLFVARDIFLVFLAVQTVIAAIAGLAYLLDKDGAFRNSFDDSWDRILSKHPIPFYYCXX 616
           FRLFVARD+ LVF+AVQT+IAAI G AY++DKDGAFRNSF D WDRILSKHPIPFYYC  
Sbjct: 121 FRLFVARDVLLVFIAVQTLIAAIGGYAYIMDKDGAFRNSFSDGWDRILSKHPIPFYYCIG 180

Query: 617 XXXXXXXXXXXXXXXHCSAFNGSDPRMAACQNCCYGWGILDCFPASMEACXXXXXXXXXX 796
                          HCS+ N +DPR+A C NCCYGWGILDCFPASMEAC          
Sbjct: 181 VLAFFVLLGFFGLILHCSSLNSNDPRVAGCHNCCYGWGILDCFPASMEACFALVIVFVVI 240

Query: 797 XXXXXXXYAFLAATMAIQRIWQRHYHILTKRELTQEYVVKDLHGCYTPPKLDAEHEE 967
                  Y FLAATMAIQRIWQRHYHILTKRELT+EYVV+DLHGCYTPPKL+ EHE+
Sbjct: 241 FVILGIAYGFLAATMAIQRIWQRHYHILTKRELTKEYVVEDLHGCYTPPKLEPEHEQ 297


>ref|XP_003609239.1| E3 ubiquitin-protein ligase MARCH3 [Medicago truncatula]
           gi|355510294|gb|AES91436.1| E3 ubiquitin-protein ligase
           MARCH3 [Medicago truncatula]
          Length = 312

 Score =  412 bits (1059), Expect = e-113
 Identities = 197/305 (64%), Positives = 219/305 (71%), Gaps = 10/305 (3%)
 Frame = +2

Query: 86  MNGELQLETLA-DRNPSDLDPLI---------DNHNRGXXXXXXXXXXXXXGSVPCCRIC 235
           M GE+QL+     +NPSD DPL+         D+H  G             GS+PCCRIC
Sbjct: 1   MLGEVQLQPPPIQQNPSDSDPLLAHQDEVEDDDSHGSGNSNEIKDQEDIEAGSLPCCRIC 60

Query: 236 LESGDNDDDDLISPCMCKGTQQFVHRMCLDHWRSVKEGFAFSHCTTCKAQFHLEVVKLED 415
           LES  + +D+LISPCMCKGTQQFVHR CLDHWRSVKEGFAFSHCTTCKAQFHL V   ED
Sbjct: 61  LESDSDPEDELISPCMCKGTQQFVHRSCLDHWRSVKEGFAFSHCTTCKAQFHLRVETYED 120

Query: 416 SSWRKMKFRLFVARDIFLVFLAVQTVIAAIAGLAYLLDKDGAFRNSFDDSWDRILSKHPI 595
           +SWRK+KFRLFVARD+FLVFLAVQTVIAAI G AY++DKDG FRNSFDD WDRILS+HPI
Sbjct: 121 NSWRKIKFRLFVARDVFLVFLAVQTVIAAIGGFAYIMDKDGNFRNSFDDGWDRILSRHPI 180

Query: 596 PFYYCXXXXXXXXXXXXXXXXXHCSAFNGSDPRMAACQNCCYGWGILDCFPASMEACXXX 775
           PFYYC                 HCS+ N +DPRMA CQNCCYGWGILDCFPASMEAC   
Sbjct: 181 PFYYCIGVVAFFVLIGFFGLILHCSSLNSNDPRMAGCQNCCYGWGILDCFPASMEACFAL 240

Query: 776 XXXXXXXXXXXXXXYAFLAATMAIQRIWQRHYHILTKRELTQEYVVKDLHGCYTPPKLDA 955
                         Y FLA+TMAIQRIWQRHYHILTKRELT+EY+V+DL G Y PPK+D 
Sbjct: 241 VVVFVVIFAILGVAYGFLASTMAIQRIWQRHYHILTKRELTKEYIVEDLQGSYFPPKIDP 300

Query: 956 EHEER 970
           EHE R
Sbjct: 301 EHESR 305


>gb|AFK48121.1| unknown [Lotus japonicus]
          Length = 307

 Score =  408 bits (1048), Expect = e-111
 Identities = 195/300 (65%), Positives = 218/300 (72%), Gaps = 5/300 (1%)
 Frame = +2

Query: 86  MNGELQLETLAD-RNPSDLDPLIDNHNRGXXXXXXXXXXXXX----GSVPCCRICLESGD 250
           M GE+QL+     +NPSD DPL+ +H+                   GS+PCCRICLES  
Sbjct: 1   MKGEVQLQPPPIMQNPSDSDPLLLDHDEDESPLSSAEIKDEEDVEAGSLPCCRICLESDS 60

Query: 251 NDDDDLISPCMCKGTQQFVHRMCLDHWRSVKEGFAFSHCTTCKAQFHLEVVKLEDSSWRK 430
           + +D+LISPCMCKGTQQFVHR CLDHWRSVKEGFAFSHCTTCKAQFHL V   ED+SWRK
Sbjct: 61  DPEDELISPCMCKGTQQFVHRSCLDHWRSVKEGFAFSHCTTCKAQFHLRVESYEDNSWRK 120

Query: 431 MKFRLFVARDIFLVFLAVQTVIAAIAGLAYLLDKDGAFRNSFDDSWDRILSKHPIPFYYC 610
           +KFRLFVARD+FLVF+AVQTVIAAI G AY++DKDG FRNSFDD WDRILSKHPIPFYYC
Sbjct: 121 IKFRLFVARDVFLVFVAVQTVIAAIGGFAYIMDKDGTFRNSFDDGWDRILSKHPIPFYYC 180

Query: 611 XXXXXXXXXXXXXXXXXHCSAFNGSDPRMAACQNCCYGWGILDCFPASMEACXXXXXXXX 790
                            HCS+ N +DPRMA CQNCCYGWGILDCFPAS+EAC        
Sbjct: 181 IGVLAFFVLIGFFGLILHCSSLNSNDPRMAGCQNCCYGWGILDCFPASVEACFALVVVFV 240

Query: 791 XXXXXXXXXYAFLAATMAIQRIWQRHYHILTKRELTQEYVVKDLHGCYTPPKLDAEHEER 970
                    Y FLA TMAIQRIWQR YHILTKRELT+EY+V+DL GCY+PPKLD EHE R
Sbjct: 241 VIFAILGIAYGFLATTMAIQRIWQRLYHILTKRELTKEYIVEDLGGCYSPPKLDPEHEAR 300


>ref|NP_001241911.1| uncharacterized protein LOC100818950 [Glycine max]
           gi|255641889|gb|ACU21213.1| unknown [Glycine max]
          Length = 309

 Score =  408 bits (1048), Expect = e-111
 Identities = 195/302 (64%), Positives = 217/302 (71%), Gaps = 7/302 (2%)
 Frame = +2

Query: 86  MNGELQLETLAD-RNPSDLDPLIDNHNR------GXXXXXXXXXXXXXGSVPCCRICLES 244
           M GE+QL+  A  +NPSD DPL+ N                       G +PCCRICLES
Sbjct: 1   MKGEVQLQPPAVMQNPSDSDPLLHNQEEEDGSPGSSGEIKNEEEDVEAGLLPCCRICLES 60

Query: 245 GDNDDDDLISPCMCKGTQQFVHRMCLDHWRSVKEGFAFSHCTTCKAQFHLEVVKLEDSSW 424
             + +D+LISPCMCKGTQQFVHR CLDHWRSVKEGFAFSHCTTCKAQFHL V   ED+SW
Sbjct: 61  DSDPEDELISPCMCKGTQQFVHRSCLDHWRSVKEGFAFSHCTTCKAQFHLRVESFEDNSW 120

Query: 425 RKMKFRLFVARDIFLVFLAVQTVIAAIAGLAYLLDKDGAFRNSFDDSWDRILSKHPIPFY 604
           RK+KFRLFVARD+FLVFLAVQTVIAAI G +Y++DKDG+FRNSFDD WDRILS+HPIPFY
Sbjct: 121 RKIKFRLFVARDVFLVFLAVQTVIAAIGGFSYIMDKDGSFRNSFDDGWDRILSRHPIPFY 180

Query: 605 YCXXXXXXXXXXXXXXXXXHCSAFNGSDPRMAACQNCCYGWGILDCFPASMEACXXXXXX 784
           YC                 HCS+ N +DPRMA CQNCCYGW +LDCFPASMEAC      
Sbjct: 181 YCIGVLAFFVLIGFFGLILHCSSLNSNDPRMAGCQNCCYGWCLLDCFPASMEACFALVVV 240

Query: 785 XXXXXXXXXXXYAFLAATMAIQRIWQRHYHILTKRELTQEYVVKDLHGCYTPPKLDAEHE 964
                      Y FLAATMAIQRIWQRHYHILTKRELT+EY+V+DL GCY PPKLD EHE
Sbjct: 241 FVVIFAILGIAYGFLAATMAIQRIWQRHYHILTKRELTKEYIVEDLGGCYFPPKLDPEHE 300

Query: 965 ER 970
            R
Sbjct: 301 GR 302