BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00008592
         (1394 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002281380.1| PREDICTED: probable N(2),N(2)-dimethylguanos...   694   0.0  
ref|XP_003544005.1| PREDICTED: probable N(2),N(2)-dimethylguanos...   691   0.0  
ref|XP_003554958.1| PREDICTED: probable N(2),N(2)-dimethylguanos...   688   0.0  
ref|XP_003554957.1| PREDICTED: probable N(2),N(2)-dimethylguanos...   688   0.0  
ref|XP_002306060.1| predicted protein [Populus trichocarpa] gi|2...   686   0.0  

>ref|XP_002281380.1| PREDICTED: probable N(2),N(2)-dimethylguanosine tRNA
            methyltransferase 2 [Vitis vinifera]
            gi|147815608|emb|CAN65976.1| hypothetical protein
            VITISV_036159 [Vitis vinifera]
            gi|298204459|emb|CBI16939.3| unnamed protein product
            [Vitis vinifera]
          Length = 579

 Score =  694 bits (1792), Expect = 0.0
 Identities = 328/367 (89%), Positives = 352/367 (95%)
 Frame = -1

Query: 1394 HPKEFDVVDLDPYGSPSVFLDSAVQSIADGGILMCTATDMAVLCGGNGEVCYSKYGSYPL 1215
            HPKEFDVVDLDPYGSPSVFLDSAVQ +ADGG+LMCTATDMAVLCGGNGE CYSKYGSYPL
Sbjct: 197  HPKEFDVVDLDPYGSPSVFLDSAVQCVADGGMLMCTATDMAVLCGGNGEACYSKYGSYPL 256

Query: 1214 RAKYCHEMALRILLACIESHANRYKRYIVPILSVQMDFYVRVFVRVYTSASAMKNTPLKL 1035
            R KYCHEMALRILLA IESHANRYKRYIVP+LSVQMDFYVRVFVR+YTSASAMK+TPLKL
Sbjct: 257  RGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVFVRIYTSASAMKSTPLKL 316

Query: 1034 SYIYQCVGCDSFHLQPIGRTVSKNNSVRYLPGFGPVVPQECSDCGKKYNMGGPIWSAPIH 855
            SY+YQC GCDSFHLQPIGRTVSKNNSVRYLPGFGPVVPQECSDCGKK+NMGGPIWSAPIH
Sbjct: 317  SYVYQCTGCDSFHLQPIGRTVSKNNSVRYLPGFGPVVPQECSDCGKKFNMGGPIWSAPIH 376

Query: 854  DQDWVSSILEDVKAMKNRYPAYNRISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAV 675
            DQ+WV+SIL DVK+MK+RYPAYNRISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAV
Sbjct: 377  DQEWVTSILADVKSMKDRYPAYNRISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAV 436

Query: 674  IFRSAVINAGYRISGTHVNPLGLKSDAPMNVIWDIMRCWVKSHPVKAQPSEHAGSIILAK 495
            IFRSAV+NAGYRISGTHVNPLG+KSDAPM+VIWDIMRCWVK+HPVKAQ  + +GS+ILAK
Sbjct: 437  IFRSAVLNAGYRISGTHVNPLGMKSDAPMDVIWDIMRCWVKNHPVKAQQPDQSGSVILAK 496

Query: 494  EPELQANFARAVASLSKAQAKKIARFLPNPESHWGPKLRAGRTITSKHVSLLGPKAINGT 315
            EP LQANFARAVASLSKAQ KK+ARFLPNPE HWGPKLRAGR ITSKHVSLLGP+A+NG+
Sbjct: 497  EPVLQANFARAVASLSKAQTKKVARFLPNPERHWGPKLRAGRQITSKHVSLLGPEAVNGS 556

Query: 314  TNHKEGD 294
             NH+E +
Sbjct: 557  MNHQEDE 563


>ref|XP_003544005.1| PREDICTED: probable N(2),N(2)-dimethylguanosine tRNA
            methyltransferase 1-like [Glycine max]
          Length = 582

 Score =  691 bits (1783), Expect = 0.0
 Identities = 327/367 (89%), Positives = 349/367 (95%)
 Frame = -1

Query: 1394 HPKEFDVVDLDPYGSPSVFLDSAVQSIADGGILMCTATDMAVLCGGNGEVCYSKYGSYPL 1215
            HPKEFDVVDLDPYGSPSVFLDSAVQS  DGG+LMCTATDMAVLCGGNGEVCYSKYGSYPL
Sbjct: 212  HPKEFDVVDLDPYGSPSVFLDSAVQSTVDGGMLMCTATDMAVLCGGNGEVCYSKYGSYPL 271

Query: 1214 RAKYCHEMALRILLACIESHANRYKRYIVPILSVQMDFYVRVFVRVYTSASAMKNTPLKL 1035
            R KYCHEMALRI+LACIESHANRYKRYIVP+LSVQMDFY+RVFVR+YTSASAMKNTPLKL
Sbjct: 272  RGKYCHEMALRIVLACIESHANRYKRYIVPVLSVQMDFYLRVFVRIYTSASAMKNTPLKL 331

Query: 1034 SYIYQCVGCDSFHLQPIGRTVSKNNSVRYLPGFGPVVPQECSDCGKKYNMGGPIWSAPIH 855
            SY+YQC GCDSFHLQPIGRT+SKN SVRYLPGFGPVVPQECSDCGKK+NMGGPIWSAPIH
Sbjct: 332  SYVYQCTGCDSFHLQPIGRTISKNTSVRYLPGFGPVVPQECSDCGKKFNMGGPIWSAPIH 391

Query: 854  DQDWVSSILEDVKAMKNRYPAYNRISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAV 675
            DQ+WV++IL DVK MK+RYPAY+RISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSA+
Sbjct: 392  DQEWVAAILADVKYMKDRYPAYDRISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAI 451

Query: 674  IFRSAVINAGYRISGTHVNPLGLKSDAPMNVIWDIMRCWVKSHPVKAQPSEHAGSIILAK 495
            IFRSAVINAGYRISGTHVNPLGLKSDAPM+VIWDIMRCWVK+HPVK QP++  GSIILAK
Sbjct: 452  IFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKPQPADQPGSIILAK 511

Query: 494  EPELQANFARAVASLSKAQAKKIARFLPNPESHWGPKLRAGRTITSKHVSLLGPKAINGT 315
            EP LQANFARAVASLSKAQAKK+ARFLPNPE HWGPKLRAGR ITSKHVSLLGP AING 
Sbjct: 512  EPALQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQITSKHVSLLGPDAINGA 571

Query: 314  TNHKEGD 294
             N ++ +
Sbjct: 572  LNQEDDE 578


>ref|XP_003554958.1| PREDICTED: probable N(2),N(2)-dimethylguanosine tRNA
            methyltransferase 1-like isoform 2 [Glycine max]
          Length = 645

 Score =  688 bits (1775), Expect = 0.0
 Identities = 324/367 (88%), Positives = 350/367 (95%)
 Frame = -1

Query: 1394 HPKEFDVVDLDPYGSPSVFLDSAVQSIADGGILMCTATDMAVLCGGNGEVCYSKYGSYPL 1215
            H KEFDVVDLDPYGSPS+FLDSAVQS+ DGG+LMCTATDMAVLCGGNGEVCYSKYGSYPL
Sbjct: 264  HAKEFDVVDLDPYGSPSMFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVCYSKYGSYPL 323

Query: 1214 RAKYCHEMALRILLACIESHANRYKRYIVPILSVQMDFYVRVFVRVYTSASAMKNTPLKL 1035
            R KYCHEMALRI+LACIESHANRYKRYIVP+LSVQMDFY+RVFVR+YTSASAMKNTPLKL
Sbjct: 324  RGKYCHEMALRIVLACIESHANRYKRYIVPVLSVQMDFYLRVFVRIYTSASAMKNTPLKL 383

Query: 1034 SYIYQCVGCDSFHLQPIGRTVSKNNSVRYLPGFGPVVPQECSDCGKKYNMGGPIWSAPIH 855
            SY+YQC GCDSFHLQPIGRT+SKN SVRYLPGFGPVVPQEC DCGKK+NMGGPIWSAPIH
Sbjct: 384  SYVYQCTGCDSFHLQPIGRTISKNTSVRYLPGFGPVVPQECRDCGKKFNMGGPIWSAPIH 443

Query: 854  DQDWVSSILEDVKAMKNRYPAYNRISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAV 675
            DQ+WV++IL DVK+MK+RYPAY+RISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSA+
Sbjct: 444  DQEWVAAILADVKSMKDRYPAYDRISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAI 503

Query: 674  IFRSAVINAGYRISGTHVNPLGLKSDAPMNVIWDIMRCWVKSHPVKAQPSEHAGSIILAK 495
            IFRSAVINAGYRISGTHVNPLGLKSDAPM+VIWDIMRCWVK+HPVK QP++  GSIILAK
Sbjct: 504  IFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKPQPADQPGSIILAK 563

Query: 494  EPELQANFARAVASLSKAQAKKIARFLPNPESHWGPKLRAGRTITSKHVSLLGPKAINGT 315
            EP LQANFARAVASLSKAQAKK+ARFLPNPE HWGPKLRAGR ITSKHVSLLGP+AING 
Sbjct: 564  EPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQITSKHVSLLGPEAINGA 623

Query: 314  TNHKEGD 294
             N ++ +
Sbjct: 624  LNQEDDE 630


>ref|XP_003554957.1| PREDICTED: probable N(2),N(2)-dimethylguanosine tRNA
            methyltransferase 1-like isoform 1 [Glycine max]
          Length = 638

 Score =  688 bits (1775), Expect = 0.0
 Identities = 324/367 (88%), Positives = 350/367 (95%)
 Frame = -1

Query: 1394 HPKEFDVVDLDPYGSPSVFLDSAVQSIADGGILMCTATDMAVLCGGNGEVCYSKYGSYPL 1215
            H KEFDVVDLDPYGSPS+FLDSAVQS+ DGG+LMCTATDMAVLCGGNGEVCYSKYGSYPL
Sbjct: 257  HAKEFDVVDLDPYGSPSMFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVCYSKYGSYPL 316

Query: 1214 RAKYCHEMALRILLACIESHANRYKRYIVPILSVQMDFYVRVFVRVYTSASAMKNTPLKL 1035
            R KYCHEMALRI+LACIESHANRYKRYIVP+LSVQMDFY+RVFVR+YTSASAMKNTPLKL
Sbjct: 317  RGKYCHEMALRIVLACIESHANRYKRYIVPVLSVQMDFYLRVFVRIYTSASAMKNTPLKL 376

Query: 1034 SYIYQCVGCDSFHLQPIGRTVSKNNSVRYLPGFGPVVPQECSDCGKKYNMGGPIWSAPIH 855
            SY+YQC GCDSFHLQPIGRT+SKN SVRYLPGFGPVVPQEC DCGKK+NMGGPIWSAPIH
Sbjct: 377  SYVYQCTGCDSFHLQPIGRTISKNTSVRYLPGFGPVVPQECRDCGKKFNMGGPIWSAPIH 436

Query: 854  DQDWVSSILEDVKAMKNRYPAYNRISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAV 675
            DQ+WV++IL DVK+MK+RYPAY+RISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSA+
Sbjct: 437  DQEWVAAILADVKSMKDRYPAYDRISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAI 496

Query: 674  IFRSAVINAGYRISGTHVNPLGLKSDAPMNVIWDIMRCWVKSHPVKAQPSEHAGSIILAK 495
            IFRSAVINAGYRISGTHVNPLGLKSDAPM+VIWDIMRCWVK+HPVK QP++  GSIILAK
Sbjct: 497  IFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKPQPADQPGSIILAK 556

Query: 494  EPELQANFARAVASLSKAQAKKIARFLPNPESHWGPKLRAGRTITSKHVSLLGPKAINGT 315
            EP LQANFARAVASLSKAQAKK+ARFLPNPE HWGPKLRAGR ITSKHVSLLGP+AING 
Sbjct: 557  EPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQITSKHVSLLGPEAINGA 616

Query: 314  TNHKEGD 294
             N ++ +
Sbjct: 617  LNQEDDE 623


>ref|XP_002306060.1| predicted protein [Populus trichocarpa] gi|222849024|gb|EEE86571.1|
            predicted protein [Populus trichocarpa]
          Length = 582

 Score =  686 bits (1769), Expect = 0.0
 Identities = 324/367 (88%), Positives = 351/367 (95%)
 Frame = -1

Query: 1394 HPKEFDVVDLDPYGSPSVFLDSAVQSIADGGILMCTATDMAVLCGGNGEVCYSKYGSYPL 1215
            HPKEFDVVDLDPYGSPSVFLDSAVQS+ DGG+LMCTATDMAVLCGGNGEVCYSKYGSYPL
Sbjct: 197  HPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVCYSKYGSYPL 256

Query: 1214 RAKYCHEMALRILLACIESHANRYKRYIVPILSVQMDFYVRVFVRVYTSASAMKNTPLKL 1035
            R KYCHEMALRILLA IESHANRYKRYIVP+LSVQM+FYVRVFVRVYTSASAMKNTPLKL
Sbjct: 257  RGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMNFYVRVFVRVYTSASAMKNTPLKL 316

Query: 1034 SYIYQCVGCDSFHLQPIGRTVSKNNSVRYLPGFGPVVPQECSDCGKKYNMGGPIWSAPIH 855
            SY+YQC+GCDS+HLQPIGRT+SK +SVR+LPGFGPVVPQECSDCGKKYNMGGPIWSAPIH
Sbjct: 317  SYVYQCIGCDSYHLQPIGRTMSKGSSVRHLPGFGPVVPQECSDCGKKYNMGGPIWSAPIH 376

Query: 854  DQDWVSSILEDVKAMKNRYPAYNRISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAV 675
            DQ+WV+S+L+ VK+MK+RYPAY+RISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAV
Sbjct: 377  DQEWVTSVLDGVKSMKDRYPAYDRISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAV 436

Query: 674  IFRSAVINAGYRISGTHVNPLGLKSDAPMNVIWDIMRCWVKSHPVKAQPSEHAGSIILAK 495
            IFRSAVINAGYRISGTHVNPLGLKSDAPM+VIWDIMRCWVK+HPVKAQ  +  GSIILAK
Sbjct: 437  IFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKAQAPDQPGSIILAK 496

Query: 494  EPELQANFARAVASLSKAQAKKIARFLPNPESHWGPKLRAGRTITSKHVSLLGPKAINGT 315
            EP LQANFARAVASLSKAQAKK+ARFLPNPE HWGPKLRAGR IT+KH+SLLGP+A+NG 
Sbjct: 497  EPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQITTKHISLLGPEAVNGH 556

Query: 314  TNHKEGD 294
             NH+  +
Sbjct: 557  LNHENSE 563