BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00008577
         (1358 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002326766.1| predicted protein [Populus trichocarpa] gi|1...   402   0.0  
ref|XP_002265127.1| PREDICTED: probable methyltransferase PMT20 ...   402   0.0  
emb|CBI37452.3| unnamed protein product [Vitis vinifera]              402   0.0  
ref|XP_002304375.1| predicted protein [Populus trichocarpa] gi|2...   396   0.0  
ref|XP_002519866.1| S-adenosylmethionine-dependent methyltransfe...   387   0.0  

>ref|XP_002326766.1| predicted protein [Populus trichocarpa] gi|118486237|gb|ABK94960.1|
           unknown [Populus trichocarpa]
           gi|222834088|gb|EEE72565.1| predicted protein [Populus
           trichocarpa]
          Length = 599

 Score =  402 bits (1033), Expect(2) = 0.0
 Identities = 190/254 (74%), Positives = 205/254 (80%)
 Frame = +3

Query: 6   IYCPEKDRIVASDVSKAVESSNAAVPVALQVKSVSFPECGLDYQDYTPCTDPKRWRKYDV 185
           I+C EKD+I A DV+K V S   +    LQ+KS +FPEC  DYQDYTPCTDPKRW+KY  
Sbjct: 38  IFCSEKDKIEAKDVAKVVSSPKESSIAPLQIKSTAFPECSSDYQDYTPCTDPKRWKKYGN 97

Query: 186 HRLTFMERHCPPLFERKECLVPPPDGYKVPIKWPTSRNECWYRNVPYDWINKQKSNQHWL 365
           HRLTFMERHCPP+FERKECL+PPPDGYK PIKWP SR++CWYRNVPYDWINKQKSNQ+WL
Sbjct: 98  HRLTFMERHCPPVFERKECLIPPPDGYKPPIKWPKSRDQCWYRNVPYDWINKQKSNQNWL 157

Query: 366 QKEGEKFRFPGGGTMFPNGVGAYVDLMQDLIPGMKDGTIRTAIDTGCGVASWGGDLLXXX 545
           +KEG+KF FPGGGTMFP GVGAYVDLMQDLIP MKDGTIRTAIDTGCGVASWGGDLL   
Sbjct: 158 RKEGDKFLFPGGGTMFPRGVGAYVDLMQDLIPEMKDGTIRTAIDTGCGVASWGGDLL--- 214

Query: 546 XXXXXXXXXXXXXXXXXXXXXXXXDRGILTVSLAPRDNHEAQVQFALERGIPAILGIIST 725
                                   DRGILTVSLAPRDNHEAQVQFALERGIPAILGIIST
Sbjct: 215 ------------------------DRGILTVSLAPRDNHEAQVQFALERGIPAILGIIST 250

Query: 726 QRLPFPSNSFDMAH 767
           QRLPFPSN+FDMAH
Sbjct: 251 QRLPFPSNAFDMAH 264



 Score =  297 bits (761), Expect(2) = 0.0
 Identities = 130/165 (78%), Positives = 147/165 (89%), Gaps = 1/165 (0%)
 Frame = +1

Query: 865  WAEFGGIYLLEVHRILRPGGFWVLSGPPVNYENRWRGWNTTVEDQKSDYEKLQDLLTSLC 1044
            W EFGGIYLLEVHRILRPGGFWVLSGPPVNYENRWRGWNTTVE+QKSDYEKLQ+LLTS+C
Sbjct: 272  WTEFGGIYLLEVHRILRPGGFWVLSGPPVNYENRWRGWNTTVEEQKSDYEKLQELLTSMC 331

Query: 1045 FKLFDKKDDIAVWQKSSDDSCYKKLDSPDVYPPKCDDGTEPDSAWYTPLRPCVVVPNKK- 1221
            FKL+DKKDDIAVWQK+SD+SCY KL + D YPPKCDD  EPDSAWYTP+RPCVVVP+ K 
Sbjct: 332  FKLYDKKDDIAVWQKASDNSCYSKLANTDAYPPKCDDSLEPDSAWYTPIRPCVVVPSPKI 391

Query: 1222 RKIALPSLPKWPERLHTAPERVADVRGGSDGSFNHDDSKWKVRAK 1356
            +K  + S+PKWPERLH  PER++D+ GGS  +F HDDSKWK+RAK
Sbjct: 392  KKSVMESIPKWPERLHATPERISDIPGGSASAFKHDDSKWKIRAK 436


>ref|XP_002265127.1| PREDICTED: probable methyltransferase PMT20 [Vitis vinifera]
          Length = 600

 Score =  402 bits (1032), Expect(2) = 0.0
 Identities = 188/254 (74%), Positives = 206/254 (81%)
 Frame = +3

Query: 6   IYCPEKDRIVASDVSKAVESSNAAVPVALQVKSVSFPECGLDYQDYTPCTDPKRWRKYDV 185
           I+C EK+R V  +V KAV+S   +    LQ+KSV+FPEC  +YQDYTPCTDP++W+KY +
Sbjct: 38  IFCSEKERFVTKEVEKAVQSPKESSSSPLQIKSVAFPECSREYQDYTPCTDPRKWKKYGL 97

Query: 186 HRLTFMERHCPPLFERKECLVPPPDGYKVPIKWPTSRNECWYRNVPYDWINKQKSNQHWL 365
           HRLTFMERHCPP+FERKECL+PPPDGYK PIKWP SRNECWYRNVPYDWINKQKSNQ+WL
Sbjct: 98  HRLTFMERHCPPVFERKECLIPPPDGYKPPIKWPKSRNECWYRNVPYDWINKQKSNQNWL 157

Query: 366 QKEGEKFRFPGGGTMFPNGVGAYVDLMQDLIPGMKDGTIRTAIDTGCGVASWGGDLLXXX 545
           +KEGEKF FPGGGTMFP GVGAYVDLMQDLIP M DGT+RTAIDTGCGVASWGGDLL   
Sbjct: 158 RKEGEKFLFPGGGTMFPRGVGAYVDLMQDLIPEMLDGTVRTAIDTGCGVASWGGDLL--- 214

Query: 546 XXXXXXXXXXXXXXXXXXXXXXXXDRGILTVSLAPRDNHEAQVQFALERGIPAILGIIST 725
                                   DRGILT+SLAPRDNHEAQVQFALERGIPAILGIIST
Sbjct: 215 ------------------------DRGILTMSLAPRDNHEAQVQFALERGIPAILGIIST 250

Query: 726 QRLPFPSNSFDMAH 767
           QRLPFPSNSFDMAH
Sbjct: 251 QRLPFPSNSFDMAH 264



 Score =  297 bits (761), Expect(2) = 0.0
 Identities = 131/165 (79%), Positives = 149/165 (90%), Gaps = 1/165 (0%)
 Frame = +1

Query: 865  WAEFGGIYLLEVHRILRPGGFWVLSGPPVNYENRWRGWNTTVEDQKSDYEKLQDLLTSLC 1044
            W EFGGIYLLEVHRILRPGGFWVLSGPPVNYENRWRGWNTTVEDQKSDYEKL +LLTS+C
Sbjct: 272  WTEFGGIYLLEVHRILRPGGFWVLSGPPVNYENRWRGWNTTVEDQKSDYEKLTELLTSMC 331

Query: 1045 FKLFDKKDDIAVWQKSSDDSCYKKLDSPDVYPPKCDDGTEPDSAWYTPLRPCVVVPNKK- 1221
            FKL++KKDDIAVWQKSSD++CY++L SPDVYPPKCDDGTEPD+AWYTPLRPCVVVP  K 
Sbjct: 332  FKLYNKKDDIAVWQKSSDNNCYQQLSSPDVYPPKCDDGTEPDAAWYTPLRPCVVVPEPKY 391

Query: 1222 RKIALPSLPKWPERLHTAPERVADVRGGSDGSFNHDDSKWKVRAK 1356
            +K+ L S+PKWPERL+ AP+R++ + GGS  +F HDDSKWK R K
Sbjct: 392  KKLGLKSVPKWPERLNVAPDRISAIHGGSASTFKHDDSKWKERLK 436


>emb|CBI37452.3| unnamed protein product [Vitis vinifera]
          Length = 577

 Score =  402 bits (1032), Expect(2) = 0.0
 Identities = 188/254 (74%), Positives = 206/254 (81%)
 Frame = +3

Query: 6   IYCPEKDRIVASDVSKAVESSNAAVPVALQVKSVSFPECGLDYQDYTPCTDPKRWRKYDV 185
           I+C EK+R V  +V KAV+S   +    LQ+KSV+FPEC  +YQDYTPCTDP++W+KY +
Sbjct: 15  IFCSEKERFVTKEVEKAVQSPKESSSSPLQIKSVAFPECSREYQDYTPCTDPRKWKKYGL 74

Query: 186 HRLTFMERHCPPLFERKECLVPPPDGYKVPIKWPTSRNECWYRNVPYDWINKQKSNQHWL 365
           HRLTFMERHCPP+FERKECL+PPPDGYK PIKWP SRNECWYRNVPYDWINKQKSNQ+WL
Sbjct: 75  HRLTFMERHCPPVFERKECLIPPPDGYKPPIKWPKSRNECWYRNVPYDWINKQKSNQNWL 134

Query: 366 QKEGEKFRFPGGGTMFPNGVGAYVDLMQDLIPGMKDGTIRTAIDTGCGVASWGGDLLXXX 545
           +KEGEKF FPGGGTMFP GVGAYVDLMQDLIP M DGT+RTAIDTGCGVASWGGDLL   
Sbjct: 135 RKEGEKFLFPGGGTMFPRGVGAYVDLMQDLIPEMLDGTVRTAIDTGCGVASWGGDLL--- 191

Query: 546 XXXXXXXXXXXXXXXXXXXXXXXXDRGILTVSLAPRDNHEAQVQFALERGIPAILGIIST 725
                                   DRGILT+SLAPRDNHEAQVQFALERGIPAILGIIST
Sbjct: 192 ------------------------DRGILTMSLAPRDNHEAQVQFALERGIPAILGIIST 227

Query: 726 QRLPFPSNSFDMAH 767
           QRLPFPSNSFDMAH
Sbjct: 228 QRLPFPSNSFDMAH 241



 Score =  297 bits (761), Expect(2) = 0.0
 Identities = 131/165 (79%), Positives = 149/165 (90%), Gaps = 1/165 (0%)
 Frame = +1

Query: 865  WAEFGGIYLLEVHRILRPGGFWVLSGPPVNYENRWRGWNTTVEDQKSDYEKLQDLLTSLC 1044
            W EFGGIYLLEVHRILRPGGFWVLSGPPVNYENRWRGWNTTVEDQKSDYEKL +LLTS+C
Sbjct: 249  WTEFGGIYLLEVHRILRPGGFWVLSGPPVNYENRWRGWNTTVEDQKSDYEKLTELLTSMC 308

Query: 1045 FKLFDKKDDIAVWQKSSDDSCYKKLDSPDVYPPKCDDGTEPDSAWYTPLRPCVVVPNKK- 1221
            FKL++KKDDIAVWQKSSD++CY++L SPDVYPPKCDDGTEPD+AWYTPLRPCVVVP  K 
Sbjct: 309  FKLYNKKDDIAVWQKSSDNNCYQQLSSPDVYPPKCDDGTEPDAAWYTPLRPCVVVPEPKY 368

Query: 1222 RKIALPSLPKWPERLHTAPERVADVRGGSDGSFNHDDSKWKVRAK 1356
            +K+ L S+PKWPERL+ AP+R++ + GGS  +F HDDSKWK R K
Sbjct: 369  KKLGLKSVPKWPERLNVAPDRISAIHGGSASTFKHDDSKWKERLK 413


>ref|XP_002304375.1| predicted protein [Populus trichocarpa] gi|222841807|gb|EEE79354.1|
           predicted protein [Populus trichocarpa]
          Length = 580

 Score =  396 bits (1018), Expect(2) = 0.0
 Identities = 187/254 (73%), Positives = 203/254 (79%)
 Frame = +3

Query: 6   IYCPEKDRIVASDVSKAVESSNAAVPVALQVKSVSFPECGLDYQDYTPCTDPKRWRKYDV 185
           I+C E+D+I   DV+K V S   +    LQ+KS +FPEC  DYQDYTPCTDP+RW+KY  
Sbjct: 18  IFCSERDKIEVKDVAKVVSSPKESSVAPLQIKSTAFPECSSDYQDYTPCTDPRRWKKYGN 77

Query: 186 HRLTFMERHCPPLFERKECLVPPPDGYKVPIKWPTSRNECWYRNVPYDWINKQKSNQHWL 365
           HRLTFMERHCPP+FERKECLVPPP+GYK PI WP SR++CWYRNVPYDWINKQKSNQ+WL
Sbjct: 78  HRLTFMERHCPPVFERKECLVPPPEGYKPPITWPKSRDQCWYRNVPYDWINKQKSNQNWL 137

Query: 366 QKEGEKFRFPGGGTMFPNGVGAYVDLMQDLIPGMKDGTIRTAIDTGCGVASWGGDLLXXX 545
           +KEGEKF FPGGGTMFP GVGAYVDLMQDLIP MKDGTIRTAIDTGCGVASWGGDLL   
Sbjct: 138 RKEGEKFLFPGGGTMFPRGVGAYVDLMQDLIPEMKDGTIRTAIDTGCGVASWGGDLL--- 194

Query: 546 XXXXXXXXXXXXXXXXXXXXXXXXDRGILTVSLAPRDNHEAQVQFALERGIPAILGIIST 725
                                   DRGILTVSLAPRDNHEAQVQFALERGIPAILGIIST
Sbjct: 195 ------------------------DRGILTVSLAPRDNHEAQVQFALERGIPAILGIIST 230

Query: 726 QRLPFPSNSFDMAH 767
           QRLPFPSN+FDMAH
Sbjct: 231 QRLPFPSNAFDMAH 244



 Score =  295 bits (755), Expect(2) = 0.0
 Identities = 129/165 (78%), Positives = 144/165 (87%), Gaps = 1/165 (0%)
 Frame = +1

Query: 865  WAEFGGIYLLEVHRILRPGGFWVLSGPPVNYENRWRGWNTTVEDQKSDYEKLQDLLTSLC 1044
            W EFGGIYLLEVHRILRPGGFWVLSGPPVNYEN WRGWNTTVE+QKSDYEKLQ+LLTS+C
Sbjct: 252  WTEFGGIYLLEVHRILRPGGFWVLSGPPVNYENHWRGWNTTVEEQKSDYEKLQELLTSMC 311

Query: 1045 FKLFDKKDDIAVWQKSSDDSCYKKLDSPDVYPPKCDDGTEPDSAWYTPLRPCVVVPNKK- 1221
            FKL+DKKDDIAVWQK+SD+SCY KL  PD YPPKCDD  EPDSAWYTP RPCVVVP+ + 
Sbjct: 312  FKLYDKKDDIAVWQKASDNSCYSKLTYPDAYPPKCDDSLEPDSAWYTPFRPCVVVPSPRI 371

Query: 1222 RKIALPSLPKWPERLHTAPERVADVRGGSDGSFNHDDSKWKVRAK 1356
            +K  + S+PKWP+RLH  PER+ DV GGS  +F HDDSKWK+RAK
Sbjct: 372  KKSVMESIPKWPQRLHVTPERILDVHGGSASAFKHDDSKWKIRAK 416


>ref|XP_002519866.1| S-adenosylmethionine-dependent methyltransferase, putative [Ricinus
           communis] gi|223540912|gb|EEF42470.1|
           S-adenosylmethionine-dependent methyltransferase,
           putative [Ricinus communis]
          Length = 501

 Score =  387 bits (995), Expect(2) = 0.0
 Identities = 183/254 (72%), Positives = 201/254 (79%)
 Frame = +3

Query: 6   IYCPEKDRIVASDVSKAVESSNAAVPVALQVKSVSFPECGLDYQDYTPCTDPKRWRKYDV 185
           I+C +++RI  SDV K V S        LQ KS +FPEC  +YQDYTPCTDP++W+KY +
Sbjct: 38  IFCSDRNRIEISDVPKDVASPKETAVAPLQTKSTAFPECSSEYQDYTPCTDPRKWKKYGL 97

Query: 186 HRLTFMERHCPPLFERKECLVPPPDGYKVPIKWPTSRNECWYRNVPYDWINKQKSNQHWL 365
            RLTFMERHCPP+FERKECL+PPPDGYK PIKWP SR++CWYRNVPYDWINKQKSNQ+WL
Sbjct: 98  QRLTFMERHCPPVFERKECLIPPPDGYKSPIKWPKSRDQCWYRNVPYDWINKQKSNQNWL 157

Query: 366 QKEGEKFRFPGGGTMFPNGVGAYVDLMQDLIPGMKDGTIRTAIDTGCGVASWGGDLLXXX 545
           +KEGEKF FPGGGTMFP GVGAYVDLM DLIP MKDGTIRTAIDTGCGVASWGGDLL   
Sbjct: 158 RKEGEKFLFPGGGTMFPRGVGAYVDLMVDLIPEMKDGTIRTAIDTGCGVASWGGDLL--- 214

Query: 546 XXXXXXXXXXXXXXXXXXXXXXXXDRGILTVSLAPRDNHEAQVQFALERGIPAILGIIST 725
                                   DRGILT+SLAPRDNHEAQVQFALERGIPAILGIIST
Sbjct: 215 ------------------------DRGILTLSLAPRDNHEAQVQFALERGIPAILGIIST 250

Query: 726 QRLPFPSNSFDMAH 767
           QRLPFPS+SFDMAH
Sbjct: 251 QRLPFPSSSFDMAH 264



 Score =  293 bits (750), Expect(2) = 0.0
 Identities = 127/165 (76%), Positives = 148/165 (89%), Gaps = 1/165 (0%)
 Frame = +1

Query: 865  WAEFGGIYLLEVHRILRPGGFWVLSGPPVNYENRWRGWNTTVEDQKSDYEKLQDLLTSLC 1044
            W E+GGIYLLE++RILRPGGFWVLSGPPVNYENRWRGWNTT+E+QKSDYEKL++LLT++C
Sbjct: 272  WTEYGGIYLLEINRILRPGGFWVLSGPPVNYENRWRGWNTTIEEQKSDYEKLEELLTAMC 331

Query: 1045 FKLFDKKDDIAVWQKSSDDSCYKKLDSPDVYPPKCDDGTEPDSAWYTPLRPCVVVPN-KK 1221
            FKL++KKDDIAVWQK+SD SC+ KL +PD YPPKCDD  EPDSAWYTPLRPCVVVP+ K 
Sbjct: 332  FKLYNKKDDIAVWQKASDSSCFSKLANPDAYPPKCDDSLEPDSAWYTPLRPCVVVPSPKH 391

Query: 1222 RKIALPSLPKWPERLHTAPERVADVRGGSDGSFNHDDSKWKVRAK 1356
            +K  L S+PKWPERLH APER++D+ GGS  +F HDDSKWKVRAK
Sbjct: 392  KKSVLESIPKWPERLHVAPERISDLHGGSASTFKHDDSKWKVRAK 436