BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00008558
         (1983 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002281595.1| PREDICTED: E3 ubiquitin-protein ligase At4g1...   385   e-111
ref|XP_003546278.1| PREDICTED: E3 ubiquitin-protein ligase At4g1...   370   e-106
emb|CBI28122.3| unnamed protein product [Vitis vinifera]              366   e-105
ref|XP_003543658.1| PREDICTED: E3 ubiquitin-protein ligase At4g1...   365   e-105
ref|XP_002517522.1| ring finger protein, putative [Ricinus commu...   360   e-104

>ref|XP_002281595.1| PREDICTED: E3 ubiquitin-protein ligase At4g11680-like [Vitis
            vinifera]
          Length = 427

 Score =  385 bits (990), Expect(2) = e-111
 Identities = 208/377 (55%), Positives = 251/377 (66%)
 Frame = -2

Query: 1799 MASSSPKAVIENRTDSYPLLMEQPEDHESNDHVIDIERRXXXXXXXXXXXXXXXXSLGFH 1620
            MA SS +   E++TD YPLLME+PE++E+++H++DI                   S   H
Sbjct: 1    MAVSSQEPSCESQTDRYPLLMERPENYENHEHIVDIVSASDASSSSSSDEDSAHVSNPTH 60

Query: 1619 HNEERSSSSPRATITRSPLSTSNLQNPPSPSVMRRGEGYGRRHWSPFNTMLWISIELAFV 1440
            H E R  +     I++S  S+S++    + S++ R  G GRR WSPFN+ LWISIE+ F 
Sbjct: 61   H-ENRPLTDAWVPISQSSPSSSDVSESRNSSIITRAHGPGRRRWSPFNSGLWISIEIVFT 119

Query: 1439 LGQIIATIIVLALSRHENPQTPLFAWVVGYATGCVASLPLLYWRYLHRNQAPEQGSTQLQ 1260
               I+A+I+VL+LSR E PQTPLFAWVVGYA GC ASLP+L+WRYL RN   EQG   + 
Sbjct: 120  TSLIVASIVVLSLSRDEKPQTPLFAWVVGYAAGCAASLPILFWRYLRRNHDGEQGLNHML 179

Query: 1259 QDSSQSNPHAEPNSYITISLTRPPDEEEGQNNFSRTQNNQRINVDNPRLIALVDQFKMAL 1080
            Q SSQ +   E NSYITISLTR   EE   +  + T N   +   N RL  L D +KMAL
Sbjct: 180  QGSSQGSTTLESNSYITISLTRSAAEENLPDASTNTWNGHTMGASNARLSVLADYYKMAL 239

Query: 1079 DCFFAVWFVVGNVWIFGGNASAADAPNLYRLCIVFLTFSCIGYAMPFILCAMXXXXXXXX 900
            DCFFAVWFVVGNVWIFGG++S   APNLYRLCIVFLTFSCIGYAMPFILCAM        
Sbjct: 240  DCFFAVWFVVGNVWIFGGHSSFTAAPNLYRLCIVFLTFSCIGYAMPFILCAMICCCLPCI 299

Query: 899  XXXLGVREDVNQMRGASEESINALPKHKFQLKRNGXXXXXXXXXSGVDEGGIVAAGTEKE 720
               LG   D++Q+RGAS ESINALP +KF+LK+NG         SGV EGG+V AGTEKE
Sbjct: 300  ISILG-HGDLSQVRGASAESINALPTYKFKLKKNG-SGNSGEISSGV-EGGVVGAGTEKE 356

Query: 719  RVISGEDAVCCICLARY 669
            R ISGEDAVCCICL RY
Sbjct: 357  RAISGEDAVCCICLTRY 373



 Score = 45.8 bits (107), Expect(2) = e-111
 Identities = 20/35 (57%), Positives = 26/35 (74%)
 Frame = -1

Query: 594 CVDKWLKINASCPLCKFEIVGSNENPSSTVDPNQQ 490
           CVDKWLKINASCPLCK E+  +  +  S+ + +QQ
Sbjct: 392 CVDKWLKINASCPLCKCEVGENTGSLPSSSNSSQQ 426


>ref|XP_003546278.1| PREDICTED: E3 ubiquitin-protein ligase At4g11680-like [Glycine max]
          Length = 424

 Score =  370 bits (951), Expect(2) = e-106
 Identities = 196/365 (53%), Positives = 244/365 (66%)
 Frame = -2

Query: 1763 RTDSYPLLMEQPEDHESNDHVIDIERRXXXXXXXXXXXXXXXXSLGFHHNEERSSSSPRA 1584
            +TD +PLLMEQPE H  + HVIDI R                  L    NE++++   + 
Sbjct: 13   QTDQHPLLMEQPETHNGHQHVIDITRNGEALTTSSRNDQYSEMHLP--QNEDQAAGDAQN 70

Query: 1583 TITRSPLSTSNLQNPPSPSVMRRGEGYGRRHWSPFNTMLWISIELAFVLGQIIATIIVLA 1404
            +  ++  S++   N  + + +RRGEGYG    SP N+ LWIS+EL   + QIIA+I+VL+
Sbjct: 71   SSHQTSSSSAPRLNSRNSASLRRGEGYGHHGRSPLNSGLWISVELVVTVSQIIASIVVLS 130

Query: 1403 LSRHENPQTPLFAWVVGYATGCVASLPLLYWRYLHRNQAPEQGSTQLQQDSSQSNPHAEP 1224
            LSR+ENPQ PLFAW+VGYA+GCVA+LP+LYWR+ +RNQ+ EQ ++Q  Q SS SNP    
Sbjct: 131  LSRNENPQAPLFAWIVGYASGCVATLPILYWRFRNRNQSNEQDTSQASQGSSGSNP--PD 188

Query: 1223 NSYITISLTRPPDEEEGQNNFSRTQNNQRINVDNPRLIALVDQFKMALDCFFAVWFVVGN 1044
             SY +I ++   DEE G    S ++N         RL  LVD FKMALDCFFAVWFVVGN
Sbjct: 189  RSYNSIHVSHVSDEENGHATRSASRNTIMPGAFTSRLNGLVDHFKMALDCFFAVWFVVGN 248

Query: 1043 VWIFGGNASAADAPNLYRLCIVFLTFSCIGYAMPFILCAMXXXXXXXXXXXLGVREDVNQ 864
            VWIFGG+ S +DAP LYRLCIVFLTFSCIGYAMPFILCA            LG+RED +Q
Sbjct: 249  VWIFGGHTSPSDAPQLYRLCIVFLTFSCIGYAMPFILCATICCCLPCIISVLGIREDFSQ 308

Query: 863  MRGASEESINALPKHKFQLKRNGXXXXXXXXXSGVDEGGIVAAGTEKERVISGEDAVCCI 684
             RGA+ ESINALP  KF+LK N          + +DEGGI+AAGTEKER+ISGEDAVCCI
Sbjct: 309  NRGATVESINALPIFKFKLK-NNENGDDQDVNAAIDEGGILAAGTEKERMISGEDAVCCI 367

Query: 683  CLARY 669
            CLA+Y
Sbjct: 368  CLAKY 372



 Score = 44.3 bits (103), Expect(2) = e-106
 Identities = 18/23 (78%), Positives = 20/23 (86%)
 Frame = -1

Query: 594 CVDKWLKINASCPLCKFEIVGSN 526
           CVDKWLKINA+CPLCK E+  SN
Sbjct: 391 CVDKWLKINATCPLCKNEVGTSN 413


>emb|CBI28122.3| unnamed protein product [Vitis vinifera]
          Length = 407

 Score =  366 bits (940), Expect(2) = e-105
 Identities = 197/357 (55%), Positives = 237/357 (66%)
 Frame = -2

Query: 1739 MEQPEDHESNDHVIDIERRXXXXXXXXXXXXXXXXSLGFHHNEERSSSSPRATITRSPLS 1560
            ME+PE++E+++H++DI                   S   HH E R  +     I++S  S
Sbjct: 1    MERPENYENHEHIVDIVSASDASSSSSSDEDSAHVSNPTHH-ENRPLTDAWVPISQSSPS 59

Query: 1559 TSNLQNPPSPSVMRRGEGYGRRHWSPFNTMLWISIELAFVLGQIIATIIVLALSRHENPQ 1380
            +S++    + S++ R  G GRR WSPFN+ LWISIE+ F    I+A+I+VL+LSR E PQ
Sbjct: 60   SSDVSESRNSSIITRAHGPGRRRWSPFNSGLWISIEIVFTTSLIVASIVVLSLSRDEKPQ 119

Query: 1379 TPLFAWVVGYATGCVASLPLLYWRYLHRNQAPEQGSTQLQQDSSQSNPHAEPNSYITISL 1200
            TPLFAWVVGYA GC ASLP+L+WRYL RN   EQG   + Q SSQ +   E NSYITISL
Sbjct: 120  TPLFAWVVGYAAGCAASLPILFWRYLRRNHDGEQGLNHMLQGSSQGSTTLESNSYITISL 179

Query: 1199 TRPPDEEEGQNNFSRTQNNQRINVDNPRLIALVDQFKMALDCFFAVWFVVGNVWIFGGNA 1020
            TR   EE   +  + T N   +   N RL  L D +KMALDCFFAVWFVVGNVWIFGG++
Sbjct: 180  TRSAAEENLPDASTNTWNGHTMGASNARLSVLADYYKMALDCFFAVWFVVGNVWIFGGHS 239

Query: 1019 SAADAPNLYRLCIVFLTFSCIGYAMPFILCAMXXXXXXXXXXXLGVREDVNQMRGASEES 840
            S   APNLYRLCIVFLTFSCIGYAMPFILCAM           LG   D++Q+RGAS ES
Sbjct: 240  SFTAAPNLYRLCIVFLTFSCIGYAMPFILCAMICCCLPCIISILG-HGDLSQVRGASAES 298

Query: 839  INALPKHKFQLKRNGXXXXXXXXXSGVDEGGIVAAGTEKERVISGEDAVCCICLARY 669
            INALP +KF+LK+NG         SGV EGG+V AGTEKER ISGEDAVCCICL RY
Sbjct: 299  INALPTYKFKLKKNG-SGNSGEISSGV-EGGVVGAGTEKERAISGEDAVCCICLTRY 353



 Score = 45.8 bits (107), Expect(2) = e-105
 Identities = 20/35 (57%), Positives = 26/35 (74%)
 Frame = -1

Query: 594 CVDKWLKINASCPLCKFEIVGSNENPSSTVDPNQQ 490
           CVDKWLKINASCPLCK E+  +  +  S+ + +QQ
Sbjct: 372 CVDKWLKINASCPLCKCEVGENTGSLPSSSNSSQQ 406


>ref|XP_003543658.1| PREDICTED: E3 ubiquitin-protein ligase At4g11680-like [Glycine max]
          Length = 419

 Score =  365 bits (937), Expect(2) = e-105
 Identities = 194/365 (53%), Positives = 242/365 (66%)
 Frame = -2

Query: 1763 RTDSYPLLMEQPEDHESNDHVIDIERRXXXXXXXXXXXXXXXXSLGFHHNEERSSSSPRA 1584
            +TD +PLLMEQPE    + HVIDI R                  L    N+++ +   + 
Sbjct: 13   QTDQHPLLMEQPETRNGHQHVIDITRNGEALTTSYRNDQHSEMHLT--QNQDQPAGDAQD 70

Query: 1583 TITRSPLSTSNLQNPPSPSVMRRGEGYGRRHWSPFNTMLWISIELAFVLGQIIATIIVLA 1404
            +  ++  S++   N  + + +RRG+GYG R  SP N+ LWIS+EL   + QIIA+I+VL+
Sbjct: 71   SSHQTTSSSAPRLNSRNSASLRRGDGYGHRGRSPLNSGLWISVELVVTVSQIIASIVVLS 130

Query: 1403 LSRHENPQTPLFAWVVGYATGCVASLPLLYWRYLHRNQAPEQGSTQLQQDSSQSNPHAEP 1224
            LSR+ENPQ PLFAW+VGY +GCVA+LP+LYWR+ +RNQ+ EQ ++Q  Q SS SNP    
Sbjct: 131  LSRNENPQAPLFAWIVGYGSGCVATLPILYWRFRNRNQSNEQDTSQASQGSSGSNP--PD 188

Query: 1223 NSYITISLTRPPDEEEGQNNFSRTQNNQRINVDNPRLIALVDQFKMALDCFFAVWFVVGN 1044
             SY +I ++   DEE G    S ++N         RL  LVD FKMALDCFFAVWFVVGN
Sbjct: 189  RSYTSIYVSHVSDEENGHATQSASRNTIMPGAFTSRLNGLVDHFKMALDCFFAVWFVVGN 248

Query: 1043 VWIFGGNASAADAPNLYRLCIVFLTFSCIGYAMPFILCAMXXXXXXXXXXXLGVREDVNQ 864
            VWIFGG+ S +DAP LYRLCIVFLTFSCIGYAMPFILCA            LG+RED +Q
Sbjct: 249  VWIFGGHTSPSDAPQLYRLCIVFLTFSCIGYAMPFILCATICCCLPCIISVLGIREDFSQ 308

Query: 863  MRGASEESINALPKHKFQLKRNGXXXXXXXXXSGVDEGGIVAAGTEKERVISGEDAVCCI 684
             RGA+ ESINALP  KF+LK N          S +DEGGI+AAGTEKER+ISGEDAVCCI
Sbjct: 309  NRGATVESINALPIFKFKLK-NNENGDDQDANSAIDEGGILAAGTEKERMISGEDAVCCI 367

Query: 683  CLARY 669
            CLA+Y
Sbjct: 368  CLAKY 372



 Score = 44.3 bits (103), Expect(2) = e-105
 Identities = 18/23 (78%), Positives = 20/23 (86%)
 Frame = -1

Query: 594 CVDKWLKINASCPLCKFEIVGSN 526
           CVDKWLKINA+CPLCK E+  SN
Sbjct: 391 CVDKWLKINATCPLCKNEVGTSN 413


>ref|XP_002517522.1| ring finger protein, putative [Ricinus communis]
            gi|223543154|gb|EEF44686.1| ring finger protein, putative
            [Ricinus communis]
          Length = 421

 Score =  360 bits (923), Expect(2) = e-104
 Identities = 193/375 (51%), Positives = 250/375 (66%)
 Frame = -2

Query: 1793 SSSPKAVIENRTDSYPLLMEQPEDHESNDHVIDIERRXXXXXXXXXXXXXXXXSLGFHHN 1614
            S  P + I  + D YPLLME+ + H+ ++H+I I                    L +H  
Sbjct: 5    SFEPNSTIPTQIDQYPLLMERMDSHDDHEHIISITGNEDGSSNSSHNGQPPDIDLPYH-- 62

Query: 1613 EERSSSSPRATITRSPLSTSNLQNPPSPSVMRRGEGYGRRHWSPFNTMLWISIELAFVLG 1434
            E+RSSS+ +     S  S+ N  N  + +  RRG+GY RR  SPFN+ LWISIEL   +G
Sbjct: 63   EDRSSSAHQ-----SSSSSLNRLNSRNSTFARRGDGYDRRRRSPFNSGLWISIELFVTVG 117

Query: 1433 QIIATIIVLALSRHENPQTPLFAWVVGYATGCVASLPLLYWRYLHRNQAPEQGSTQLQQD 1254
            QIIA+I+VL+LSR+ENPQ PLFAWVVGYA+GCVA+LP+LYWR+ +RNQ+ +Q S Q  Q 
Sbjct: 118  QIIASIVVLSLSRNENPQAPLFAWVVGYASGCVATLPILYWRFRNRNQSGQQDSHQSHQG 177

Query: 1253 SSQSNPHAEPNSYITISLTRPPDEEEGQNNFSRTQNNQRINVDNPRLIALVDQFKMALDC 1074
            SSQ+NP  + +SY  IS+T+  +E+  ++  +   N Q     + RL  L+D FKMALDC
Sbjct: 178  SSQNNP-PDTSSYTAISVTQASEEDNFRSELA-IANGQTGRPLSSRLNGLMDHFKMALDC 235

Query: 1073 FFAVWFVVGNVWIFGGNASAADAPNLYRLCIVFLTFSCIGYAMPFILCAMXXXXXXXXXX 894
            FFAVWFVVGNVWIFGG+++  DAP LY+LCIVFLTFSCIGYAMPFILCA           
Sbjct: 236  FFAVWFVVGNVWIFGGHSTPTDAPKLYKLCIVFLTFSCIGYAMPFILCATICCCLPCIIS 295

Query: 893  XLGVREDVNQMRGASEESINALPKHKFQLKRNGXXXXXXXXXSGVDEGGIVAAGTEKERV 714
             LG RED +Q RGA+ ESIN+LP +KF+L+++          SG  EGG++A GTEKER 
Sbjct: 296  LLGFREDFSQTRGATTESINSLPIYKFKLQKS--ESVNDHDNSGTGEGGVLAEGTEKERA 353

Query: 713  ISGEDAVCCICLARY 669
            ISGEDA+CCICL +Y
Sbjct: 354  ISGEDAICCICLNKY 368



 Score = 48.5 bits (114), Expect(2) = e-104
 Identities = 22/35 (62%), Positives = 25/35 (71%)
 Frame = -1

Query: 594 CVDKWLKINASCPLCKFEIVGSNENPSSTVDPNQQ 490
           CVDKWLKINASCPLCK E+  S+    S  D +QQ
Sbjct: 387 CVDKWLKINASCPLCKSELGESSSAQPSARDSSQQ 421