BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00008548
         (1682 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002266914.2| PREDICTED: vacuolar amino acid transporter 1...   721   0.0  
ref|XP_004136638.1| PREDICTED: vacuolar amino acid transporter 1...   700   0.0  
ref|XP_003518096.1| PREDICTED: vacuolar amino acid transporter 1...   670   0.0  
ref|XP_003537196.1| PREDICTED: vacuolar amino acid transporter 1...   668   0.0  
ref|XP_002881760.1| amino acid transporter family protein [Arabi...   657   0.0  

>ref|XP_002266914.2| PREDICTED: vacuolar amino acid transporter 1-like [Vitis vinifera]
          Length = 537

 Score =  721 bits (1862), Expect = 0.0
 Identities = 358/541 (66%), Positives = 436/541 (80%), Gaps = 5/541 (0%)
 Frame = -2

Query: 1612 ERDRGESEYFLENEEDD-IECNNSVDGTSFTGSDVDDDDNEVVHGSG--KSPTSTFSSQQ 1442
            E+   +SE+    E+D   +  N ++ +S         D EV +G     SP+  FSSQQ
Sbjct: 5    EKQERDSEFLFVIEDDGGDDVVNRIESSS--------GDEEVAYGDEGYTSPSVVFSSQQ 56

Query: 1441 WPRSYREATDSYTIAASPNFGLIRRSSTFRPSFYDSSARTSFEDVSKTSLLGEYDKNYKR 1262
            WP+SYRE TDSYTIAASPNFG++  S   R S  D   +++ +   KT LL E +KNY++
Sbjct: 57   WPQSYRETTDSYTIAASPNFGILGSSLGLRRSSLDIFTKSNLDLDGKTPLLTEQEKNYQK 116

Query: 1261 EELDRVSRS--SWLEKASLHEDFSGELPIGHGCSLTQTIFNGVNVLAGVGILSTPYTIKE 1088
            E+ DR+SR+  S  EKAS HE  +GELPI +GCS TQT+FNGVNVLAG+G+LSTPY +KE
Sbjct: 117  EDTDRISRTQLSLSEKASFHEQLTGELPISYGCSFTQTVFNGVNVLAGIGLLSTPYAVKE 176

Query: 1087 GGWASLGVLVLFAVICCYTASLMRHCFESRKGILSYPDIGEAAFGKYGRLFISIVLYMEL 908
             GWASL VLVLFAV+CCYTASL+R+C ES++GI++YPDIGEAAFG+YGRLF+S VLY EL
Sbjct: 177  AGWASLAVLVLFAVVCCYTASLLRYCLESKEGIITYPDIGEAAFGRYGRLFVSFVLYTEL 236

Query: 907  YSYCVEFITLEGDNLTRLFPGTSLNWACFNLDSMHLFGILTAIAVLPTVWLRDIRVISYL 728
            YSYCVEFI LEGDNLTRLFPG SLNW  F LDS+HLFGILTA+ VLPTVWLRD+RVIS L
Sbjct: 237  YSYCVEFIILEGDNLTRLFPGASLNWGGFQLDSLHLFGILTALIVLPTVWLRDLRVISCL 296

Query: 727  SAGGVLATVVIVICVLFLGTINGIGFHESGQVVNFSRMPIAIGVYGFCYSGHAVFPNIYQ 548
            SAGGVLATV+IV+ V+F+G   GIGFH++G++VN+  +P +IGVYGFC+SGH VFPNIYQ
Sbjct: 297  SAGGVLATVLIVVSVIFVGITAGIGFHQTGKLVNWGGIPFSIGVYGFCFSGHTVFPNIYQ 356

Query: 547  SMADKTKFTKALIVSFSLCVLLYGSVAIMGFLMFGEDTLSQITLNMPYHFFASKVALWTT 368
            SMADKTKF+KALIVS  LCV++YG +AI+GFLMFG+ T+SQITLN+P H FASK+ALWTT
Sbjct: 357  SMADKTKFSKALIVSILLCVIIYGGIAIVGFLMFGQGTMSQITLNIPRHVFASKIALWTT 416

Query: 367  VVNPLTKYALLMNPLARSIEELLPVGVSSSIWCFILLRTALVASSVCVAFLLPFFGLVMA 188
            V+NP TKYALLMNPLARSIEELLPV +S+S WCFILLRTALV SSVCVAFLLPFFGLVM+
Sbjct: 417  VINPFTKYALLMNPLARSIEELLPVRISNSFWCFILLRTALVISSVCVAFLLPFFGLVMS 476

Query: 187  LIGSLLSVLVAIIMPALCFLRILGPKATSVQKIMSTTIVVVGIATAILGTYSSLLEIMKN 8
            LIGSLLSVLV++I+P LC+LRI+G KAT  Q I+S+ +  +G+  AILGTYSSL +I + 
Sbjct: 477  LIGSLLSVLVSVIIPTLCYLRIMGNKATKAQVIVSSGVAALGVICAILGTYSSLSQIARQ 536

Query: 7    Y 5
            Y
Sbjct: 537  Y 537


>ref|XP_004136638.1| PREDICTED: vacuolar amino acid transporter 1-like [Cucumis sativus]
          Length = 539

 Score =  700 bits (1807), Expect = 0.0
 Identities = 353/543 (65%), Positives = 437/543 (80%), Gaps = 6/543 (1%)
 Frame = -2

Query: 1615 AERDRGESEYFLENE---EDDIECNNSV-DGTSFTGSDVDDDDNEVVHGSGKSPTSTFSS 1448
            A+ +  +SE+FL+ +   +DD+E + S+ D +S T SD DD  N    G G   TSTF+S
Sbjct: 3    AKTNDPDSEFFLDGDVYADDDLERSKSMEDRSSSTYSDADDAPN----GLG-GRTSTFTS 57

Query: 1447 QQWPRSYREATDSYTIAASPNFGLIRRSSTFRPSFYDSSARTSFEDVSKTSLLGEYDKNY 1268
            QQWP+SYRE TDSYTI ASPNF + R  S  + S ++ S R+S E  +K  LL +   + 
Sbjct: 58   QQWPQSYRETTDSYTIMASPNFAMFRVPSILQTSLHNLS-RSSLEMDAKAPLLSDPGGDD 116

Query: 1267 KREELDRVSRSSWL--EKASLHEDFSGELPIGHGCSLTQTIFNGVNVLAGVGILSTPYTI 1094
            + E+   +SR   L  E+ ++ +  +GELPIG GCS TQTIFNG+NVLAGVG+LSTP+T+
Sbjct: 117  QNEDFYNISRLQSLRSERTTISKQMTGELPIGRGCSFTQTIFNGINVLAGVGLLSTPFTV 176

Query: 1093 KEGGWASLGVLVLFAVICCYTASLMRHCFESRKGILSYPDIGEAAFGKYGRLFISIVLYM 914
            KE GWASLGVLVLFA++CCYTA+LMR+CFE R+G+++YPDIGEAA+GK+GRLF+SI+LY+
Sbjct: 177  KEAGWASLGVLVLFAIVCCYTATLMRYCFEKREGVITYPDIGEAAYGKFGRLFVSIILYL 236

Query: 913  ELYSYCVEFITLEGDNLTRLFPGTSLNWACFNLDSMHLFGILTAIAVLPTVWLRDIRVIS 734
            ELY YCVEFI LEGDNLT LFPG SL+     LDSMHLFGILTA+ VLPTVWLRD+RVIS
Sbjct: 237  ELYCYCVEFIILEGDNLTSLFPGASLSLGSIQLDSMHLFGILTALIVLPTVWLRDLRVIS 296

Query: 733  YLSAGGVLATVVIVICVLFLGTINGIGFHESGQVVNFSRMPIAIGVYGFCYSGHAVFPNI 554
            YLSAGGV+AT++I +C++FLGT  G+GFH++G+VVN+  +P AIGVYGFCYSGH VFPNI
Sbjct: 297  YLSAGGVIATILIGLCLIFLGTFGGVGFHQTGEVVNWKGVPFAIGVYGFCYSGHTVFPNI 356

Query: 553  YQSMADKTKFTKALIVSFSLCVLLYGSVAIMGFLMFGEDTLSQITLNMPYHFFASKVALW 374
            YQSMADKTKFTKALIV F LCVL+YG VAIMGFLMFG++TLSQITLNMP H   SKVA W
Sbjct: 357  YQSMADKTKFTKALIVCFILCVLIYGGVAIMGFLMFGQNTLSQITLNMPKHAVTSKVAGW 416

Query: 373  TTVVNPLTKYALLMNPLARSIEELLPVGVSSSIWCFILLRTALVASSVCVAFLLPFFGLV 194
            TTV+NP TKYALLMNPLARSIEELLP  +S+S  CFILLRTALVASSVCVAF+LPFFGLV
Sbjct: 417  TTVINPFTKYALLMNPLARSIEELLPPRISASYGCFILLRTALVASSVCVAFILPFFGLV 476

Query: 193  MALIGSLLSVLVAIIMPALCFLRILGPKATSVQKIMSTTIVVVGIATAILGTYSSLLEIM 14
            M+LIGSLLS+LV++I+P +C+L+I+G  AT +Q   S  +V +GI +AI+GTYSSL +I 
Sbjct: 477  MSLIGSLLSILVSVIVPTMCYLKIMGSDATKIQITSSIVVVGLGIVSAIMGTYSSLSKIA 536

Query: 13   KNY 5
            K+Y
Sbjct: 537  KSY 539


>ref|XP_003518096.1| PREDICTED: vacuolar amino acid transporter 1-like [Glycine max]
          Length = 531

 Score =  670 bits (1729), Expect = 0.0
 Identities = 326/538 (60%), Positives = 419/538 (77%), Gaps = 2/538 (0%)
 Frame = -2

Query: 1612 ERDRGESEYFLENEEDDI--ECNNSVDGTSFTGSDVDDDDNEVVHGSGKSPTSTFSSQQW 1439
            E+D+   ++  +  +DD+  E    ++G  +      D D+     + ++   +FSSQQW
Sbjct: 10   EKDKDLDQFLFDRNDDDLVDEEQQEIEGVKYESESSSDGDD----ANRRAQPDSFSSQQW 65

Query: 1438 PRSYREATDSYTIAASPNFGLIRRSSTFRPSFYDSSARTSFEDVSKTSLLGEYDKNYKRE 1259
            P+SY+E TDSYTIAA+PNF  + R  +F  S +D+ ++++ +   KT  L          
Sbjct: 66   PQSYKETTDSYTIAAAPNFESVLRGPSFIYSSFDNRSKSNLDIDGKTPFLSA-------- 117

Query: 1258 ELDRVSRSSWLEKASLHEDFSGELPIGHGCSLTQTIFNGVNVLAGVGILSTPYTIKEGGW 1079
              + + +S+W EKAS+    SGELPIG+GCS TQTIFNG+NV+AGVG+LSTPYT+ + GW
Sbjct: 118  -AEGIRQSTWWEKASVQRLVSGELPIGYGCSFTQTIFNGINVMAGVGLLSTPYTVNQAGW 176

Query: 1078 ASLGVLVLFAVICCYTASLMRHCFESRKGILSYPDIGEAAFGKYGRLFISIVLYMELYSY 899
             S+ V++LFAV+CCYTA+L+R+CFE+R+ I++YPDIGEAAFG+YGR+ +SI+LY ELYSY
Sbjct: 177  MSMAVMLLFAVMCCYTATLLRYCFENREEIITYPDIGEAAFGRYGRIAVSIILYTELYSY 236

Query: 898  CVEFITLEGDNLTRLFPGTSLNWACFNLDSMHLFGILTAIAVLPTVWLRDIRVISYLSAG 719
            CVEFITLEGDNLT LFPGTSL+   F LDSMHLFG+LTA+ +LPTVWL+D+R+ISYLSAG
Sbjct: 237  CVEFITLEGDNLTSLFPGTSLDLGGFQLDSMHLFGVLTALIILPTVWLKDLRIISYLSAG 296

Query: 718  GVLATVVIVICVLFLGTINGIGFHESGQVVNFSRMPIAIGVYGFCYSGHAVFPNIYQSMA 539
            GV+ATV+I+ICV  +GTI+G+GFH +GQ+V ++ +P AIGVYGFC++GH+VFPNIYQSMA
Sbjct: 297  GVIATVLIIICVFCVGTIDGVGFHHTGQLVKWNGIPFAIGVYGFCFAGHSVFPNIYQSMA 356

Query: 538  DKTKFTKALIVSFSLCVLLYGSVAIMGFLMFGEDTLSQITLNMPYHFFASKVALWTTVVN 359
            DK +FTKALI+ F LCVL+YG  AIMG+LMFG+ TLSQITLNMP   FASKVALWTTV+N
Sbjct: 357  DKKQFTKALIICFVLCVLIYGGTAIMGYLMFGDGTLSQITLNMPPGTFASKVALWTTVIN 416

Query: 358  PLTKYALLMNPLARSIEELLPVGVSSSIWCFILLRTALVASSVCVAFLLPFFGLVMALIG 179
               KYALLMNPLARS+EELLP  +SSS WCFILLRT LVAS+VCVAFL+PFFGLVMALIG
Sbjct: 417  ---KYALLMNPLARSLEELLPDRISSSYWCFILLRTTLVASTVCVAFLVPFFGLVMALIG 473

Query: 178  SLLSVLVAIIMPALCFLRILGPKATSVQKIMSTTIVVVGIATAILGTYSSLLEIMKNY 5
            SL S+LV+ IMP+LCFL+I+G KAT  Q ++S  I   G+   ILGTYSSLL I  +Y
Sbjct: 474  SLFSILVSAIMPSLCFLKIIGKKATRTQVVLSVAIAAFGVICGILGTYSSLLSIADSY 531


>ref|XP_003537196.1| PREDICTED: vacuolar amino acid transporter 1-like [Glycine max]
          Length = 548

 Score =  668 bits (1724), Expect = 0.0
 Identities = 327/535 (61%), Positives = 418/535 (78%), Gaps = 1/535 (0%)
 Frame = -2

Query: 1606 DRGESEYFLENEEDDIECNNSVDGTSFTGSDVDDDDNEVVHGS-GKSPTSTFSSQQWPRS 1430
            D  E +  ++ E+ +IE    V   S + SD DDDD    H + G++   +FSSQQWP+S
Sbjct: 26   DGDEEDAVVDKEQQEIE---GVKYQSESSSDGDDDDGGGRHAANGRAQPDSFSSQQWPQS 82

Query: 1429 YREATDSYTIAASPNFGLIRRSSTFRPSFYDSSARTSFEDVSKTSLLGEYDKNYKREELD 1250
            Y+E TDSYTIAA+PNF  + R  +F  S +D+ ++++ +   KT  L   +         
Sbjct: 83   YKETTDSYTIAAAPNFESVLRGPSFIYSSFDNRSKSNLDIDGKTPFLSAPEG-------- 134

Query: 1249 RVSRSSWLEKASLHEDFSGELPIGHGCSLTQTIFNGVNVLAGVGILSTPYTIKEGGWASL 1070
             + +S+W EKAS+    SGELPIG+GCS TQTIFNG+NV+AGVG+LSTP+T+ + GW SL
Sbjct: 135  -IRQSTWWEKASVERLVSGELPIGYGCSFTQTIFNGINVIAGVGLLSTPFTVNQAGWMSL 193

Query: 1069 GVLVLFAVICCYTASLMRHCFESRKGILSYPDIGEAAFGKYGRLFISIVLYMELYSYCVE 890
             V++LFAV+CCYTA+L+R+CFESR+GI++YPD+GEAAFG+YGR+ +SI+LY ELYSYCVE
Sbjct: 194  AVMLLFAVMCCYTATLLRYCFESREGIITYPDVGEAAFGRYGRIAVSIILYTELYSYCVE 253

Query: 889  FITLEGDNLTRLFPGTSLNWACFNLDSMHLFGILTAIAVLPTVWLRDIRVISYLSAGGVL 710
            FITLEGDNLT LFPGTSL+   F LDSMH+FG+LTA+ +LPTVWL+D+R+ISYLS GGV+
Sbjct: 254  FITLEGDNLTSLFPGTSLDLGGFKLDSMHMFGVLTALIILPTVWLKDLRIISYLSGGGVV 313

Query: 709  ATVVIVICVLFLGTINGIGFHESGQVVNFSRMPIAIGVYGFCYSGHAVFPNIYQSMADKT 530
            AT++I+ICV  +GTI+ +GFH +GQ+V ++ +P AIGVYGFC++GH+VFPNIYQSMADK 
Sbjct: 314  ATILIMICVFCVGTIDSVGFHHTGQLVKWNGIPFAIGVYGFCFAGHSVFPNIYQSMADKK 373

Query: 529  KFTKALIVSFSLCVLLYGSVAIMGFLMFGEDTLSQITLNMPYHFFASKVALWTTVVNPLT 350
            +FTKALI+ F LCVL+YG  A MG+LMFG+ TLSQITLNMP   FASKVALWTTV++   
Sbjct: 374  QFTKALIICFVLCVLIYGGTASMGYLMFGDGTLSQITLNMPPGAFASKVALWTTVISLYN 433

Query: 349  KYALLMNPLARSIEELLPVGVSSSIWCFILLRTALVASSVCVAFLLPFFGLVMALIGSLL 170
            KYALLMNPLARS+EELLP  +SSS WCF+LLRT LVAS+VCVAFL+PFFGLVMALIGSL 
Sbjct: 434  KYALLMNPLARSLEELLPDRISSSYWCFMLLRTTLVASTVCVAFLVPFFGLVMALIGSLF 493

Query: 169  SVLVAIIMPALCFLRILGPKATSVQKIMSTTIVVVGIATAILGTYSSLLEIMKNY 5
            S+LV+ IMP+LCFL+I+G KAT  Q  +S  I   G+   ILGTYSSLL I  +Y
Sbjct: 494  SILVSAIMPSLCFLKIIGKKATKTQVALSVAIAAFGVICGILGTYSSLLSIADSY 548


>ref|XP_002881760.1| amino acid transporter family protein [Arabidopsis lyrata subsp.
            lyrata] gi|297327599|gb|EFH58019.1| amino acid
            transporter family protein [Arabidopsis lyrata subsp.
            lyrata]
          Length = 534

 Score =  657 bits (1696), Expect = 0.0
 Identities = 341/536 (63%), Positives = 420/536 (78%), Gaps = 7/536 (1%)
 Frame = -2

Query: 1591 EYFLENEED--DIECNNSV--DGTSFTGSDVDDDDNEVVHGSGKSPTSTFSSQQWPRSYR 1424
            ++  E++ED  D+E N+S   + +    SD+ D   +   G   +     S  QWP+S+R
Sbjct: 13   DFTFEDDEDNEDLEENSSKYENDSETDQSDLGDLPGDA-DGGDDNDEPFISQVQWPQSFR 71

Query: 1423 EATDSYTIAASPNFGLIRRSSTFRPSFYDSSARTSFEDVSKTSLLGEYDKNYKREELDRV 1244
            E TDSYTIAASP FG +R +    PSFY +S R++ +  SK  LL E     + E+ D+ 
Sbjct: 72   ETTDSYTIAASPIFGSLRSN----PSFYRAS-RSNLDVESKAPLLPE-----RHEDSDKA 121

Query: 1243 S--RSSWLEKASLHEDFSGELPIG-HGCSLTQTIFNGVNVLAGVGILSTPYTIKEGGWAS 1073
            S  +S+W  K S    F+ ELPIG +GCS+TQTIFN +NV+AGVG+LSTPYT+KE GWAS
Sbjct: 122  SATQSAWSHKGS----FADELPIGGYGCSVTQTIFNAINVMAGVGLLSTPYTVKEAGWAS 177

Query: 1072 LGVLVLFAVICCYTASLMRHCFESRKGILSYPDIGEAAFGKYGRLFISIVLYMELYSYCV 893
            + +L+LFAVICCYTA+LM+ CFE++ GI++YPDIGEAAFGKYGR+ I ++LY ELYSYCV
Sbjct: 178  MVILLLFAVICCYTATLMKDCFENKTGIITYPDIGEAAFGKYGRILICMLLYTELYSYCV 237

Query: 892  EFITLEGDNLTRLFPGTSLNWACFNLDSMHLFGILTAIAVLPTVWLRDIRVISYLSAGGV 713
            EFI LEGDNLT LFPGTSL+   F LDS HLFGILTA+ VLPTVWL+D+R+ISYLSAGGV
Sbjct: 238  EFIILEGDNLTGLFPGTSLDLLGFRLDSKHLFGILTALIVLPTVWLKDLRIISYLSAGGV 297

Query: 712  LATVVIVICVLFLGTINGIGFHESGQVVNFSRMPIAIGVYGFCYSGHAVFPNIYQSMADK 533
            +AT +I + V FLGT  GIGFH +GQ V ++ +P AIG+YGFCYSGH+VFPNIYQSMADK
Sbjct: 298  IATGLIAVSVFFLGTTGGIGFHHTGQAVKWNGIPFAIGIYGFCYSGHSVFPNIYQSMADK 357

Query: 532  TKFTKALIVSFSLCVLLYGSVAIMGFLMFGEDTLSQITLNMPYHFFASKVALWTTVVNPL 353
            TKF KA+I  F LCVLLYG VAIMG+LMFGE TLSQITLNMP + F SKVA WTTVVNP 
Sbjct: 358  TKFNKAVITCFILCVLLYGGVAIMGYLMFGEATLSQITLNMPQNQFFSKVAQWTTVVNPF 417

Query: 352  TKYALLMNPLARSIEELLPVGVSSSIWCFILLRTALVASSVCVAFLLPFFGLVMALIGSL 173
            TKYALLMNPLARSIEELLP  +S +IWCF+LLRTALVASSVC AFL+PFFGL+MALIGSL
Sbjct: 418  TKYALLMNPLARSIEELLPDRMSENIWCFLLLRTALVASSVCSAFLIPFFGLMMALIGSL 477

Query: 172  LSVLVAIIMPALCFLRILGPKATSVQKIMSTTIVVVGIATAILGTYSSLLEIMKNY 5
            LS+LVAI+MPALCF++I+G KAT  Q I+S+TIV +G+ +  LGTYSS+ +I++NY
Sbjct: 478  LSILVAIVMPALCFIKIMGNKATRTQMILSSTIVAIGLVSGTLGTYSSVAKIIRNY 533