BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00008466
         (1457 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002266888.1| PREDICTED: probable serine/threonine-protein...   607   e-171
ref|XP_004134295.1| PREDICTED: probable serine/threonine-protein...   593   e-167
ref|XP_002523302.1| kinase, putative [Ricinus communis] gi|22353...   591   e-166
ref|XP_004155253.1| PREDICTED: LOW QUALITY PROTEIN: probable ser...   590   e-166
ref|XP_002320210.1| predicted protein [Populus trichocarpa] gi|2...   583   e-164

>ref|XP_002266888.1| PREDICTED: probable serine/threonine-protein kinase WNK4-like [Vitis
            vinifera]
          Length = 625

 Score =  607 bits (1564), Expect = e-171
 Identities = 309/425 (72%), Positives = 353/425 (83%), Gaps = 5/425 (1%)
 Frame = +1

Query: 55   DDPKEVDCVEVDPKGRYVRYKEILGKGAFKTVYKAFDQLHGIEVAWNRVKIDDVLKSSVD 234
            +D  E   VE DP GRYVR+KEILGKGAFKTVYKAFD++ GIE+AWN+VKIDDVL+S  D
Sbjct: 5    EDEDEAAYVEKDPTGRYVRFKEILGKGAFKTVYKAFDEVDGIEIAWNQVKIDDVLRSPED 64

Query: 235  LEKLYSEVHLLRSVKHDNIIKFYDSWVDDKKKTVNMITELFTSGSLRQYRKKHKCVDMKA 414
            LEKLYSEVHLL+S+KH+NIIKFY+SWVDDKKKTVNMITELFTSGSLRQYRKKHK VDMKA
Sbjct: 65   LEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKTVNMITELFTSGSLRQYRKKHKNVDMKA 124

Query: 415  IKNWARQILCGLDYLHSQN--IIHRDLKCDNIFVNGNHGEIKIGDLGLATVMQQPPAKSV 588
            IKNWARQ+L GL YLHS N  IIHRDLKCDNIFVNGNHGE+KIGDLGLA VMQQP A+SV
Sbjct: 125  IKNWARQVLRGLVYLHSHNPPIIHRDLKCDNIFVNGNHGEVKIGDLGLAIVMQQPTARSV 184

Query: 589  IGTPEFMAPEMYDEEYNELVDVYSFGLCILEMVTFEYPYCECKNPAQIYKKVSSGVKPAS 768
            IGTPEFMAPE+Y+EEYNELVD+YSFG+C+LEMVTFEYPY ECKNPAQIYKKV+SG+KPAS
Sbjct: 185  IGTPEFMAPELYEEEYNELVDIYSFGMCMLEMVTFEYPYNECKNPAQIYKKVTSGIKPAS 244

Query: 769  LGKVSDPKVKEFIEKCLLPASNRLPARELLKDPFLQLEKPKETIHTQLLLPAQLPKSSSL 948
            L KV+D ++KEFI KCL PAS RLPA+ELLKDPF Q E PKE I   L LP++ PKS  L
Sbjct: 245  LCKVTDLQIKEFIVKCLAPASERLPAKELLKDPFFQSENPKEPIRVPLQLPSRSPKSIIL 304

Query: 949  LSCGPLSMDIDYEY---NHSVCTESSGRTPCSPCSPVLELQRMHQNNEFRLKGMKNPDNS 1119
               GP SMDID ++   + S  TE++G    SP  PVLE QRM++++EFRL+  K  DNS
Sbjct: 305  SKSGPFSMDIDPDHPQLSSSTSTENNG----SPDFPVLEFQRMYKSSEFRLRAKKINDNS 360

Query: 1120 IALTLRIANLSGRVKNIHFQFYLHTDTALSVASEMVEQLELADHDVAFIAEFIDYLINRI 1299
            I+LTLR  +  G VKNIHF F L TDT  SV  EMVEQLELA+H+VAFIA+FIDY+I R+
Sbjct: 361  ISLTLRTVDSYGPVKNIHFPFSLDTDTVHSVVGEMVEQLELAEHEVAFIADFIDYVIMRL 420

Query: 1300 LHGSK 1314
            L G K
Sbjct: 421  LPGWK 425


>ref|XP_004134295.1| PREDICTED: probable serine/threonine-protein kinase WNK4-like
            [Cucumis sativus]
          Length = 663

 Score =  593 bits (1529), Expect = e-167
 Identities = 297/413 (71%), Positives = 347/413 (84%), Gaps = 3/413 (0%)
 Frame = +1

Query: 73   DCVEVDPKGRYVRYKEILGKGAFKTVYKAFDQLHGIEVAWNRVKIDDVLKSSVDLEKLYS 252
            +  E DP GRYVRY EILG+GAFKTVYKAFD++ GIEVAWN+V+ID  L+S  DLEKLYS
Sbjct: 6    NAAEKDPTGRYVRYDEILGRGAFKTVYKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYS 65

Query: 253  EVHLLRSVKHDNIIKFYDSWVDDKKKTVNMITELFTSGSLRQYRKKHKCVDMKAIKNWAR 432
            EVHLL+S+KH+NIIKFY+SWVDDKKKTVNMITELFTSGSLRQYRKKHK VDMKAIKNWAR
Sbjct: 66   EVHLLKSLKHENIIKFYNSWVDDKKKTVNMITELFTSGSLRQYRKKHKHVDMKAIKNWAR 125

Query: 433  QILCGLDYLHSQN--IIHRDLKCDNIFVNGNHGEIKIGDLGLATVMQQPPAKSVIGTPEF 606
            QIL GL YLHS +  IIHRDLK DNIF+NGNHGE+KIGDLGLA VMQQP A+SVIGTPEF
Sbjct: 126  QILRGLVYLHSHDPPIIHRDLKGDNIFINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEF 185

Query: 607  MAPEMYDEEYNELVDVYSFGLCILEMVTFEYPYCECKNPAQIYKKVSSGVKPASLGKVSD 786
            MAPE+Y+EEYNELVDVYSFG+C+LEMVTFEYPY ECKNPAQI++KV+SG+KPASL KVSD
Sbjct: 186  MAPELYEEEYNELVDVYSFGMCMLEMVTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSD 245

Query: 787  PKVKEFIEKCLLPASNRLPARELLKDPFLQLEKPKETIHTQLLLPAQLPKSSSLLSCGPL 966
            P+  EFI KCL+P   RL A+ELLKD FLQ+E PKE+    L L  Q+ KS +L   GP+
Sbjct: 246  PRTMEFINKCLVPVHERLSAKELLKDSFLQVENPKESARNPLQLSNQVSKSINLPKSGPI 305

Query: 967  SMDIDYEYN-HSVCTESSGRTPCSPCSPVLELQRMHQNNEFRLKGMKNPDNSIALTLRIA 1143
            SMDID +   HS+ T +   +  SP  PV+E Q M++NNEFRL+G KN DNS+ALTLRIA
Sbjct: 306  SMDIDIDQKIHSLSTYAESNSG-SPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIA 364

Query: 1144 NLSGRVKNIHFQFYLHTDTALSVASEMVEQLELADHDVAFIAEFIDYLINRIL 1302
            + +GRV+NIHF FYL +DTALSVA+EM EQLEL +HDV FIAEFID+LI +++
Sbjct: 365  DSNGRVRNIHFTFYLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLI 417


>ref|XP_002523302.1| kinase, putative [Ricinus communis] gi|223537390|gb|EEF39018.1|
            kinase, putative [Ricinus communis]
          Length = 693

 Score =  591 bits (1523), Expect = e-166
 Identities = 300/438 (68%), Positives = 351/438 (80%), Gaps = 4/438 (0%)
 Frame = +1

Query: 13   GLLSTVNKNGLTEIDDPKEV--DCVEVDPKGRYVRYKEILGKGAFKTVYKAFDQLHGIEV 186
            G L+  + NG     +P +   D VE DP  RYVRY E+LGKGAFKTVYK FD++ GIEV
Sbjct: 8    GTLALASNNGTYSALEPPDAKGDFVETDPTSRYVRYDEVLGKGAFKTVYKGFDEVDGIEV 67

Query: 187  AWNRVKIDDVLKSSVDLEKLYSEVHLLRSVKHDNIIKFYDSWVDDKKKTVNMITELFTSG 366
            AWN+V+I DVL+S  DLEKL+SEVHLLRS+KH+NI++  +SWVDDKKKT+NMITELFTSG
Sbjct: 68   AWNQVRIADVLRSPKDLEKLHSEVHLLRSLKHENIMELCNSWVDDKKKTINMITELFTSG 127

Query: 367  SLRQYRKKHKCVDMKAIKNWARQILCGLDYLHSQN--IIHRDLKCDNIFVNGNHGEIKIG 540
            +LRQYRKKHK VDMKAIKNWARQIL GL YLH  N  IIHRDLKCDNIFVNG++G +KIG
Sbjct: 128  NLRQYRKKHKNVDMKAIKNWARQILQGLVYLHGHNPPIIHRDLKCDNIFVNGHNGVVKIG 187

Query: 541  DLGLATVMQQPPAKSVIGTPEFMAPEMYDEEYNELVDVYSFGLCILEMVTFEYPYCECKN 720
            DLGLA +MQQP A SVIGTPEFMA E+Y+EEYNEL+D+YSFG+C+LEMVTFEYPY EC+N
Sbjct: 188  DLGLAIIMQQPTATSVIGTPEFMATELYEEEYNELIDIYSFGMCMLEMVTFEYPYSECRN 247

Query: 721  PAQIYKKVSSGVKPASLGKVSDPKVKEFIEKCLLPASNRLPARELLKDPFLQLEKPKETI 900
            PAQIYKKV+SG+KPASLG VSDP+VKEFI KCL+PAS RL A+ELLKDPFLQ +   E +
Sbjct: 248  PAQIYKKVTSGIKPASLGNVSDPQVKEFILKCLVPASERLSAKELLKDPFLQPQNLVEPV 307

Query: 901  HTQLLLPAQLPKSSSLLSCGPLSMDIDYEYNHSVCTESSGRTPCSPCSPVLELQRMHQNN 1080
               LLLP Q PKSSS    GPLSMDID +Y     +  +G        PV E QR H+N 
Sbjct: 308  RDPLLLPNQYPKSSSATKSGPLSMDIDADYKQISSSTCTGSNNEGTRFPVPEYQREHKNK 367

Query: 1081 EFRLKGMKNPDNSIALTLRIANLSGRVKNIHFQFYLHTDTALSVASEMVEQLELADHDVA 1260
             F+LKG ++ DNS++LTLRIA+ SGRV NIHF FYL +DTALSVASEMV+QLELADHDVA
Sbjct: 368  VFKLKGKESDDNSVSLTLRIADSSGRVWNIHFLFYLDSDTALSVASEMVDQLELADHDVA 427

Query: 1261 FIAEFIDYLINRILHGSK 1314
            FIAEFID+LI ++  G K
Sbjct: 428  FIAEFIDHLIMKLSPGWK 445


>ref|XP_004155253.1| PREDICTED: LOW QUALITY PROTEIN: probable serine/threonine-protein
            kinase WNK4-like [Cucumis sativus]
          Length = 713

 Score =  590 bits (1522), Expect = e-166
 Identities = 296/413 (71%), Positives = 346/413 (83%), Gaps = 3/413 (0%)
 Frame = +1

Query: 73   DCVEVDPKGRYVRYKEILGKGAFKTVYKAFDQLHGIEVAWNRVKIDDVLKSSVDLEKLYS 252
            +  E DP GRYVRY EILG+GAFKTVYKAFD++ GIEVAWN+V+ID  L+S  DLEKLYS
Sbjct: 72   NAAEKDPTGRYVRYDEILGRGAFKTVYKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYS 131

Query: 253  EVHLLRSVKHDNIIKFYDSWVDDKKKTVNMITELFTSGSLRQYRKKHKCVDMKAIKNWAR 432
            EVHLL+S+KH+NIIKFY+SWVDDKKKTVNMITELFTSGSLRQYRKKHK VDMKAIKNWAR
Sbjct: 132  EVHLLKSLKHENIIKFYNSWVDDKKKTVNMITELFTSGSLRQYRKKHKHVDMKAIKNWAR 191

Query: 433  QILCGLDYLHSQN--IIHRDLKCDNIFVNGNHGEIKIGDLGLATVMQQPPAKSVIGTPEF 606
            QIL GL YLHS +  IIHRDLK DNIF+NGNHGE+KIGDLGLA VMQQP A+SVIGTPEF
Sbjct: 192  QILRGLVYLHSHDPPIIHRDLKGDNIFINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEF 251

Query: 607  MAPEMYDEEYNELVDVYSFGLCILEMVTFEYPYCECKNPAQIYKKVSSGVKPASLGKVSD 786
            MAPE+Y+EEYNELVDVYSFG+C+LEMVTFEYPY ECKNPAQI++KV+SG+KPASL KVSD
Sbjct: 252  MAPELYEEEYNELVDVYSFGMCMLEMVTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSD 311

Query: 787  PKVKEFIEKCLLPASNRLPARELLKDPFLQLEKPKETIHTQLLLPAQLPKSSSLLSCGPL 966
            P+  EFI KCL+P   RL A+ELLKD FLQ+E PKE+    L L  Q+ KS +L   GP+
Sbjct: 312  PRTMEFINKCLVPVHERLSAKELLKDSFLQVENPKESARNPLQLSNQVSKSINLPKSGPI 371

Query: 967  SMDIDYEYN-HSVCTESSGRTPCSPCSPVLELQRMHQNNEFRLKGMKNPDNSIALTLRIA 1143
            SMDID +   HS+ T +   +  SP  PV+E Q M++NNEFRL+G KN DNS+ALTLRIA
Sbjct: 372  SMDIDIDQKIHSLSTYAESNSG-SPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIA 430

Query: 1144 NLSGRVKNIHFQFYLHTDTALSVASEMVEQLELADHDVAFIAEFIDYLINRIL 1302
            + +GRV+NIHF  YL +DTALSVA+EM EQLEL +HDV FIAEFID+LI +++
Sbjct: 431  DSNGRVRNIHFTXYLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLI 483


>ref|XP_002320210.1| predicted protein [Populus trichocarpa] gi|222860983|gb|EEE98525.1|
            predicted protein [Populus trichocarpa]
          Length = 425

 Score =  583 bits (1503), Expect = e-164
 Identities = 299/420 (71%), Positives = 346/420 (82%), Gaps = 6/420 (1%)
 Frame = +1

Query: 73   DCVEVDPKGRYVRYKEILGKGAFKTVYKAFDQLHGIEVAWNRVKIDDVLKSSVDLEKLYS 252
            D VE DP GRYVRY ++LGKGAFKTVY+AFD++ GIEVAWN+V+IDDVL+S  D EKLYS
Sbjct: 9    DLVEKDPTGRYVRYDDVLGKGAFKTVYRAFDEVDGIEVAWNQVRIDDVLRSPEDFEKLYS 68

Query: 253  EVHLLRSVKHDNIIKFYDSWVDDKKKTVNMITELFTSGSLRQYRKKHKCVDMKAIKNWAR 432
            EV+LLRS++H+NIIKF +SWVDDK KT+NMITELFTSG+LRQYR+KH+ +D+KAIKNWAR
Sbjct: 69   EVYLLRSLRHENIIKFSNSWVDDKNKTINMITELFTSGNLRQYRRKHRNIDIKAIKNWAR 128

Query: 433  QILCGLDYLHSQN--IIHRDLKCDNIFVNGNHGEIKIGDLGLATVMQQPPAKSVIGTPEF 606
            QIL GL YLH  +  IIHRDLKCDNIFVNGNHGE+KIGDLGLA VMQ P AKSVIGTPEF
Sbjct: 129  QILRGLVYLHGHSPPIIHRDLKCDNIFVNGNHGEVKIGDLGLAIVMQNPTAKSVIGTPEF 188

Query: 607  MAPEMYDEEYNELVDVYSFGLCILEMVTFEYPYCECKNPAQIYKKVSSGVKPASLGKVSD 786
            MAPE+Y+EEYNELVD+YSFG+CILE+VT  YPY EC+N AQIYKKV+SG+KPASL  V +
Sbjct: 189  MAPELYEEEYNELVDIYSFGMCILELVTCNYPYSECRNAAQIYKKVTSGIKPASLKTVGN 248

Query: 787  PKVKEFIEKCLLPASNRLPARELLKDPFLQLEKP-KETIHTQLLLPAQLPKSSSLLSCGP 963
             ++KEFIEKCLLPAS RL A+ELLK PFLQLE P  E I   L +P Q P S SL   GP
Sbjct: 249  TQIKEFIEKCLLPASERLSAKELLKHPFLQLEIPILEQICDPLPVPDQFPISLSLPKSGP 308

Query: 964  LSMDIDY---EYNHSVCTESSGRTPCSPCSPVLELQRMHQNNEFRLKGMKNPDNSIALTL 1134
            L MD+D    + + S CT S+     SP SPVLE QR H+NNEF LKGMKN DNS +LTL
Sbjct: 309  LFMDMDIDEKQLSESTCTGSNNG---SPKSPVLEYQRAHKNNEFGLKGMKNDDNSASLTL 365

Query: 1135 RIANLSGRVKNIHFQFYLHTDTALSVASEMVEQLELADHDVAFIAEFIDYLINRILHGSK 1314
            RI++L GRV+NIHF FYL +DTA+SVASEMVEQLELADHDVAFIAE ID LI ++L G K
Sbjct: 366  RISDLGGRVRNIHFIFYLDSDTAISVASEMVEQLELADHDVAFIAELIDSLIVKLLPGWK 425