BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00008433
         (1241 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002520518.1| protein with unknown function [Ricinus commu...   310   6e-82
ref|XP_003543527.1| PREDICTED: transcription initiation factor I...   283   6e-74
ref|XP_002282322.1| PREDICTED: uncharacterized protein LOC100241...   283   6e-74
ref|XP_003543528.1| PREDICTED: transcription initiation factor I...   281   2e-73
ref|XP_002298340.1| predicted protein [Populus trichocarpa] gi|2...   277   5e-72

>ref|XP_002520518.1| protein with unknown function [Ricinus communis]
           gi|223540360|gb|EEF41931.1| protein with unknown
           function [Ricinus communis]
          Length = 383

 Score =  310 bits (793), Expect = 6e-82
 Identities = 167/297 (56%), Positives = 197/297 (66%), Gaps = 13/297 (4%)
 Frame = +2

Query: 5   NGGPGDAVLLELQGLWEAKMMQAGSILGPIERSAVPRPIPGGPITPVHDLNVXXXXXXXX 184
           N GPG+ VL ELQ +WE KMMQAG I GPIERS+  + +PGG ITPVHDLNV        
Sbjct: 29  NNGPGETVLNELQAIWELKMMQAGVICGPIERSSGAKTVPGGVITPVHDLNVPYEGTEEY 88

Query: 185 XXXXADXXXXXXXXXXXXXXXXXGSGQTPLPGTAQTP-----GTAELYNIPTGGTPFTPA 349
               A+                     TPLPGTAQTP       + +YNIPTG T    +
Sbjct: 89  ETPTAEILFPP----------------TPLPGTAQTPLPGSVDNSSMYNIPTGPT----S 128

Query: 350 EYP---SDNGGATEVKPGRPSQFMQPPAPWLNQRPPLDVNVAYNEGREEINRGASHEPTT 520
           EYP   SD+GG+TE K GRPS +MQPP+PW+NQRPPLDVN+AY EGREE +RG S++P T
Sbjct: 129 EYPTPVSDSGGSTEAKAGRPSPYMQPPSPWINQRPPLDVNIAYVEGREEADRGTSNQPLT 188

Query: 521 QDFFTVAAGKRKRDDFPSQYN--GYIPQQDGAGDAILD--ESKEGQVTSAANEEFGRGMR 688
           QDFFT A+GKRKR+DF +QYN  G+IPQQDGAGD   +  + +   V SA  E      R
Sbjct: 189 QDFFTGASGKRKREDFAAQYNNGGFIPQQDGAGDTPFEVLQVERDTVISADIEILSSVTR 248

Query: 689 LSSRIPQADGPTPDPYDDALSTPNIYNYQGVVNEDYNVVNTPAP-ELQAATPAPVTQ 856
              +IPQ DGP PDPYDD LSTPNIYNYQGV NEDYN+ NTPAP +LQA+TPA + Q
Sbjct: 249 RGLKIPQLDGPIPDPYDDVLSTPNIYNYQGVANEDYNIANTPAPNDLQASTPAIIPQ 305



 Score = 99.0 bits (245), Expect = 2e-18
 Identities = 45/47 (95%), Positives = 46/47 (97%)
 Frame = +2

Query: 1022 NTQHLVLAQFDKVTRTKSKWKCTLKDGIMHINNKDILFNKANGEFDF 1162
            NTQHLVLAQFDKVTRTKS+WKCTLKDGIMHINNKDILFNKA GEFDF
Sbjct: 337  NTQHLVLAQFDKVTRTKSRWKCTLKDGIMHINNKDILFNKATGEFDF 383


>ref|XP_003543527.1| PREDICTED: transcription initiation factor IIA large subunit-like
           isoform 1 [Glycine max]
          Length = 391

 Score =  283 bits (724), Expect = 6e-74
 Identities = 159/302 (52%), Positives = 192/302 (63%), Gaps = 17/302 (5%)
 Frame = +2

Query: 2   NNGGPGDAVLLELQGLWEAKMMQAGSILGPIERSAVPRPIPGGPITPVHDLNVXXXXXXX 181
           NNGGPG+ VL ELQ +WE+KMMQAG++LGPIERS   +P PGGPITPVHDLNV       
Sbjct: 27  NNGGPGEEVLKELQAMWESKMMQAGAVLGPIERSTAAKPTPGGPITPVHDLNVPYEGTDE 86

Query: 182 XXXXXADXXXXXXXXXXXXXXXXXGSGQTPLPGTAQTPGTAELYNIPTGGTPFTPAEYPS 361
                AD                    QTPLPGT    G    YNIPTG     P++YPS
Sbjct: 87  YETPTADMLFPPTPLQTPI--------QTPLPGT----GDNSTYNIPTG-----PSDYPS 129

Query: 362 ---DNGGATEVKPGRPSQFMQPPAPWLNQRPPLDVNVAYNEGREEINRGASHEPTTQDFF 532
              D GG  + K  RP+ +MQ P+PW+NQRPPLDVNVAY EGR+E +RG S++P TQDFF
Sbjct: 130 SGNDTGGNADGKGARPAPYMQSPSPWMNQRPPLDVNVAYVEGRDEADRGTSNQPPTQDFF 189

Query: 533 TVAAGKRKRDDFPSQYN--GYIPQQDGAGDAI-----LDESKEGQVTSAANEEFGRGM-- 685
           T+ +GKRKR+D  SQYN  GYIPQQDGAGDA      ++ S  G ++  ++    +G   
Sbjct: 190 TMPSGKRKRNDLTSQYNAGGYIPQQDGAGDATHGDFEIEVSGRG-ISINSHHTISKGKMS 248

Query: 686 ----RLSSRIPQADGPTPDPYDDALSTPNIYNYQGVVNEDYNVVNTPA-PELQAATPAPV 850
               R +SRIPQ DGP P   DD LSTPNIYNY  V +EDYN+ NTPA PE+ A+TPA +
Sbjct: 249 ADLERSTSRIPQLDGPIPYDDDDVLSTPNIYNYGEVFSEDYNISNTPAPPEVPASTPALL 308

Query: 851 TQ 856
            Q
Sbjct: 309 VQ 310



 Score = 95.5 bits (236), Expect = 2e-17
 Identities = 43/47 (91%), Positives = 45/47 (95%)
 Frame = +2

Query: 1022 NTQHLVLAQFDKVTRTKSKWKCTLKDGIMHINNKDILFNKANGEFDF 1162
            NT HLVLAQFDKVTRTKS+WKCTLKDGIMHINNKDILFNKA GEF+F
Sbjct: 345  NTHHLVLAQFDKVTRTKSRWKCTLKDGIMHINNKDILFNKATGEFEF 391


>ref|XP_002282322.1| PREDICTED: uncharacterized protein LOC100241598 [Vitis vinifera]
           gi|297746029|emb|CBI16085.3| unnamed protein product
           [Vitis vinifera]
          Length = 393

 Score =  283 bits (724), Expect = 6e-74
 Identities = 160/302 (52%), Positives = 190/302 (62%), Gaps = 21/302 (6%)
 Frame = +2

Query: 2   NNGGPGDAVLLELQGLWEAKMMQAGSILGPIERSAVPRPIPGGPIT--PVHDLNVXXXXX 175
           NNGGPG++VL ELQG+WE KM+QAG + GPIERS  P+   G P    PVHDLNV     
Sbjct: 28  NNGGPGESVLSELQGIWEMKMVQAGVVTGPIERSTAPKQTSGAPAPTPPVHDLNVPYEGT 87

Query: 176 XXXXXXXADXXXXXXXXXXXXXXXXXGSGQTPLPGTAQTPGTAELYNIPTGGTPFTPAEY 355
                  A+                    QTPLPG     G   +YNIPTG     P EY
Sbjct: 88  EEYETPTAEILFPPTPLQTPI--------QTPLPGM----GDNSMYNIPTG-----PTEY 130

Query: 356 PS--DNGGATEVKPGRPSQFMQPPAPWLNQRPPL--DVNVAYNEGREEINRGASHEPTTQ 523
           P+  D GGAT++K GRP  +M PP+PW+ QRPPL  DVNVAY EGR+E +RG S +P TQ
Sbjct: 131 PAAQDGGGATDMKSGRPPSYMPPPSPWMQQRPPLSVDVNVAYVEGRDEGDRGNSQQPLTQ 190

Query: 524 DFFTVAAGKRKRDDFPSQYN--GYIPQQDGAGDAILD----ESKEGQ--------VTSAA 661
           DFF +++GKRKR+DFPSQY+  GYIPQQDGAGD   +    E  +G         +T A 
Sbjct: 191 DFFMMSSGKRKREDFPSQYHTSGYIPQQDGAGDPAPEVFEVEVSQGSNSIKGHDILTKAN 250

Query: 662 NEEFGRGMRLSSRIPQADGPTPDPYDDALSTPNIYNYQGVVNEDYNVVNTPAP-ELQAAT 838
            E F +      RIPQ DGP PDPY+D LSTPNIYNYQGVVNEDYN+VNTPAP ++QA T
Sbjct: 251 REIFPQVAGSYVRIPQLDGPIPDPYEDVLSTPNIYNYQGVVNEDYNIVNTPAPNDIQAGT 310

Query: 839 PA 844
           PA
Sbjct: 311 PA 312



 Score =  102 bits (254), Expect = 2e-19
 Identities = 47/47 (100%), Positives = 47/47 (100%)
 Frame = +2

Query: 1022 NTQHLVLAQFDKVTRTKSKWKCTLKDGIMHINNKDILFNKANGEFDF 1162
            NTQHLVLAQFDKVTRTKSKWKCTLKDGIMHINNKDILFNKANGEFDF
Sbjct: 347  NTQHLVLAQFDKVTRTKSKWKCTLKDGIMHINNKDILFNKANGEFDF 393


>ref|XP_003543528.1| PREDICTED: transcription initiation factor IIA large subunit-like
           isoform 2 [Glycine max]
          Length = 384

 Score =  281 bits (720), Expect = 2e-73
 Identities = 159/295 (53%), Positives = 188/295 (63%), Gaps = 10/295 (3%)
 Frame = +2

Query: 2   NNGGPGDAVLLELQGLWEAKMMQAGSILGPIERSAVPRPIPGGPITPVHDLNVXXXXXXX 181
           NNGGPG+ VL ELQ +WE+KMMQAG++LGPIERS   +P PGGPITPVHDLNV       
Sbjct: 27  NNGGPGEEVLKELQAMWESKMMQAGAVLGPIERSTAAKPTPGGPITPVHDLNVPYEGTDE 86

Query: 182 XXXXXADXXXXXXXXXXXXXXXXXGSGQTPLPGTAQTPGTAELYNIPTGGTPFTPAEYPS 361
                AD                    QTPLPGT    G    YNIPTG     P++YPS
Sbjct: 87  YETPTADMLFPPTPLQTPI--------QTPLPGT----GDNSTYNIPTG-----PSDYPS 129

Query: 362 ---DNGGATEVKPGRPSQFMQPPAPWLNQRPPLDVNVAYNEGREEINRGASHEPTTQDFF 532
              D GG  + K  RP+ +MQ P+PW+NQRPPLDVNVAY EGR+E +RG S++P TQDFF
Sbjct: 130 SGNDTGGNADGKGARPAPYMQSPSPWMNQRPPLDVNVAYVEGRDEADRGTSNQPPTQDFF 189

Query: 533 TVAAGKRKRDDFPSQYN--GYIPQQDGAGDAILDESKEGQVTSAANEEFGRGM----RLS 694
           T+ +GKRKR+D  SQYN  GYIPQQDGAGDA   +  E +    A    G+      R +
Sbjct: 190 TMPSGKRKRNDLTSQYNAGGYIPQQDGAGDATHGDF-EIEFLKIAYISKGKMSADLERST 248

Query: 695 SRIPQADGPTPDPYDDALSTPNIYNYQGVVNEDYNVVNTPA-PELQAATPAPVTQ 856
           SRIPQ DGP P   DD LSTPNIYNY  V +EDYN+ NTPA PE+ A+TPA + Q
Sbjct: 249 SRIPQLDGPIPYDDDDVLSTPNIYNYGEVFSEDYNISNTPAPPEVPASTPALLVQ 303



 Score = 95.5 bits (236), Expect = 2e-17
 Identities = 43/47 (91%), Positives = 45/47 (95%)
 Frame = +2

Query: 1022 NTQHLVLAQFDKVTRTKSKWKCTLKDGIMHINNKDILFNKANGEFDF 1162
            NT HLVLAQFDKVTRTKS+WKCTLKDGIMHINNKDILFNKA GEF+F
Sbjct: 338  NTHHLVLAQFDKVTRTKSRWKCTLKDGIMHINNKDILFNKATGEFEF 384


>ref|XP_002298340.1| predicted protein [Populus trichocarpa] gi|222845598|gb|EEE83145.1|
           predicted protein [Populus trichocarpa]
          Length = 409

 Score =  277 bits (708), Expect = 5e-72
 Identities = 156/310 (50%), Positives = 194/310 (62%), Gaps = 25/310 (8%)
 Frame = +2

Query: 2   NNGGPGDAVLLELQGLWEAKMMQAGSILGPIERSAVPRPIPGGPITPVHDLNVXXXXXXX 181
           NNGGPG+ VL ELQGLWE K+MQAG + GPI RS+  + +  G +TPVHDLNV       
Sbjct: 27  NNGGPGETVLSELQGLWEKKLMQAGVLSGPIVRSSANKQLVPGGLTPVHDLNVPYEGTEE 86

Query: 182 XXXXXADXXXXXXXXXXXXXXXXXGSGQTPLPGTAQTP-----GTAELYNIPTGGTPFTP 346
                A+                 G+ QTPLPG   TP       + +YNI TG +    
Sbjct: 87  YETPTAEILFPPTPLPGSAQTPLPGNVQTPLPGNVPTPLPGSVDNSSMYNISTGSS---- 142

Query: 347 AEYP---SDNGGATEVKPGRPSQFMQPPAPWLNQRPPLDVNV------------AYNEGR 481
           ++YP   SD GG+T+VK GRPS FMQ P+P ++QRPPLDVNV            AY EGR
Sbjct: 143 SDYPTPVSDAGGSTDVKAGRPSHFMQSPSPLMHQRPPLDVNVGKVLTNKCCAFVAYVEGR 202

Query: 482 EEINRGASHEPTTQDFFTVAAGKRKRDDFPSQYN--GYIPQQDGAGDAILDESKE--GQV 649
           +E++RG SH+  TQDFF +++GKRKR DF  +YN  G+IPQQDGA D+  + S+     +
Sbjct: 203 DEVDRGGSHQTLTQDFF-MSSGKRKRGDFAPKYNNGGFIPQQDGAVDSASEVSQVECDTI 261

Query: 650 TSAANEEFGRGMRLSSRIPQADGPTPDPYDDALSTPNIYNYQGVVNEDYNVVNTPAP-EL 826
           T+   E   R  R   +IPQ DGP PDPYDD LSTPNIYNYQGV NEDYN+ NTPAP +L
Sbjct: 262 TTKNREILARVSRSYVKIPQVDGPIPDPYDDMLSTPNIYNYQGVANEDYNIANTPAPNDL 321

Query: 827 QAATPAPVTQ 856
           QA+TPA V+Q
Sbjct: 322 QASTPAVVSQ 331



 Score = 95.5 bits (236), Expect = 2e-17
 Identities = 41/47 (87%), Positives = 46/47 (97%)
 Frame = +2

Query: 1022 NTQHLVLAQFDKVTRTKSKWKCTLKDGIMHINNKDILFNKANGEFDF 1162
            NTQHL+LAQFDKVTRTKS+WKCTLKDG+MHINN+DILFNKA GEF+F
Sbjct: 363  NTQHLILAQFDKVTRTKSRWKCTLKDGVMHINNRDILFNKATGEFEF 409