BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00008274
         (1357 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI22579.3| unnamed protein product [Vitis vinifera]              292   2e-76
ref|XP_002266509.1| PREDICTED: protein timeless homolog [Vitis v...   288   3e-75
ref|XP_003592159.1| Topoisomerase 1-associated factor [Medicago ...   283   7e-74
ref|XP_002331831.1| predicted protein [Populus trichocarpa] gi|2...   260   5e-67
ref|XP_003555400.1| PREDICTED: protein timeless homolog [Glycine...   244   4e-62

>emb|CBI22579.3| unnamed protein product [Vitis vinifera]
          Length = 1217

 Score =  292 bits (747), Expect = 2e-76
 Identities = 184/444 (41%), Positives = 249/444 (56%), Gaps = 15/444 (3%)
 Frame = +3

Query: 3    RRMLRKMKTNPLIFVEILFWKTRKECHYINCESMLTDLTKMKKDYNKMGRTSFDGVDGSF 182
            R+MLRKMK+ PL+FVE+LFWKTR ECHYI  +S+L +L  +KK+  K G  S  G  GS 
Sbjct: 772  RKMLRKMKSQPLLFVEVLFWKTRGECHYITSQSLLHELGSLKKESGKWGNISRHGEIGST 831

Query: 183  EGKGWNRRSIADALGDDEADVMLFHET----NDHNVEKPKSSVGDVEEANKGVTSMPDEG 350
            EGKGW  RSIADALG+DEADV++ HE     ND N  + +  V  +  +     +  D  
Sbjct: 832  EGKGWMHRSIADALGEDEADVVISHEPVYQKNDDNFSEAEEGVTPISSSKIDGKTNSDNV 891

Query: 351  SNGKETMIDERYKKMKVLGSDNELCNQLKDLYEKYKDKENCIHLIVEELD--AKISYREV 524
             +  E   +   K+ + L    E+   +KDLYEK+KD  +C  LI E LD   K+S  +V
Sbjct: 892  GHYAEHESERVSKRKRRLVLSAEVEKNIKDLYEKFKDDRHCSRLIAEALDPDCKVSPVQV 951

Query: 525  SKKLKQLGFSFPQKRRLLK-----TGASNQTGEAANGFGNEITHPNLSGMHENSSVRRPM 689
            S KLKQLG     K+R+L+     + ++NQ  E A   G E  H   S   E S VR+ +
Sbjct: 952  SNKLKQLGLKIAPKKRMLQVDVPLSDSTNQLMEEARAVGEESAHLVCSNNSEGSLVRKSL 1011

Query: 690  HARKRVSAFSEDQESKIKGLFEQFKYHKRCSFMIASALDADGSFTVAQVSRKLKQLGLRA 869
            H RKRV AFS+DQE  I+ L+EQFK HKRC++MIASAL  D   T AQVSRKLKQLGL  
Sbjct: 1012 HTRKRVRAFSKDQEETIRALYEQFKGHKRCTYMIASALAGDDILTAAQVSRKLKQLGLHV 1071

Query: 870  PQKQMLNSHLQLRDEDPD--DNSDEGIDDTETLSSLSKRTKTKHNTSSVTTIPEQKLAEK 1043
            P+++    ++ LRDED +  D +     D ETL SL KR+K +++      +PEQ +  K
Sbjct: 1072 PRRKRAEGNMHLRDEDLNDFDTAKAQDSDDETLLSLRKRSKKENHRLLREEVPEQNIEGK 1131

Query: 1044 FTHEVSDDEPLSSLVGNKTKQKRGNVAERQDQKKYRKVSRXXXXXXXXXXIVGHKHEQPT 1223
               + SDDE LSS++     +K   +  + D+K+                         T
Sbjct: 1132 LM-QSSDDETLSSVL-----KKTRRLPPKLDEKR-------------------------T 1160

Query: 1224 ETANDALHIDDLEAAV--KDSSKK 1289
             T+N  L IDD   +V  KD +K+
Sbjct: 1161 STSNGRLGIDDPSGSVVAKDVTKR 1184


>ref|XP_002266509.1| PREDICTED: protein timeless homolog [Vitis vinifera]
          Length = 1269

 Score =  288 bits (736), Expect = 3e-75
 Identities = 192/461 (41%), Positives = 252/461 (54%), Gaps = 32/461 (6%)
 Frame = +3

Query: 3    RRMLRKMKTNPLIFVEILFWKTRKECHYINCESMLTDLTKMKKDYNKMGRTSFDGVDGSF 182
            R+MLRKMK+ PL+FVE+LFWKTR ECHYI  +S+L +L  +KK+  K G  S  G  GS 
Sbjct: 758  RKMLRKMKSQPLLFVEVLFWKTRGECHYITSQSLLHELGSLKKESGKWGNISRHGEIGST 817

Query: 183  EGKGWNRRSIADALGDDEADVMLFHET----NDHNVEKPKSSVGDVEEANKGVTSMPDEG 350
            EGKGW  RSIADALG+DEADV++ HE     ND N            EA +GVT +    
Sbjct: 818  EGKGWMHRSIADALGEDEADVVISHEPVYQKNDDNFS----------EAEEGVTPISSSK 867

Query: 351  SNGKETMI--------------DERYKKMK---VLGSDNELCNQLKDLYEKYKDKENCIH 479
             +GK                   ER  K K   VL ++ E    +KDLYEK+KD  +C  
Sbjct: 868  IDGKTNSDKYDVQHVGHYAEHESERVSKRKRRLVLSAEVE--KNIKDLYEKFKDDRHCSR 925

Query: 480  LIVEELD--AKISYREVSKKLKQLGFSFPQKRRLLK-----TGASNQTGEAANGFGNEIT 638
            LI E LD   K+S  +VS KLKQLG     K+R+L+     + ++NQ  E A   G E  
Sbjct: 926  LIAEALDPDCKVSPVQVSNKLKQLGLKIAPKKRMLQVDVPLSDSTNQLMEEARAVGEESA 985

Query: 639  HPNLSGMHENSSVRRPMHARKRVSAFSEDQESKIKGLFEQFKYHKRCSFMIASALDADGS 818
            H   S   E S VR+ +H RKRV AFS+DQE  I+ L+EQFK HKRC++MIASAL  D  
Sbjct: 986  HLVCSNNSEGSLVRKSLHTRKRVRAFSKDQEETIRALYEQFKGHKRCTYMIASALAGDDI 1045

Query: 819  FTVAQVSRKLKQLGLRAPQKQMLNSHLQLRDEDPD--DNSDEGIDDTETLSSLSKRTKTK 992
             T AQVSRKLKQLGL  P+++    ++ LRDED +  D +     D ETL SL KR+K +
Sbjct: 1046 LTAAQVSRKLKQLGLHVPRRKRAEGNMHLRDEDLNDFDTAKAQDSDDETLLSLRKRSKKE 1105

Query: 993  HNTSSVTTIPEQKLAEKFTHEVSDDEPLSSLVGNKTKQKRGNVAERQDQKKYRKVSRXXX 1172
            ++      +PEQ +  K   + SDDE LSS++     +K   +  + D+K+         
Sbjct: 1106 NHRLLREEVPEQNIEGKLM-QSSDDETLSSVL-----KKTRRLPPKLDEKR--------- 1150

Query: 1173 XXXXXXXIVGHKHEQPTETANDALHIDDLEAAV--KDSSKK 1289
                            T T+N  L IDD   +V  KD +K+
Sbjct: 1151 ----------------TSTSNGRLGIDDPSGSVVAKDVTKR 1175


>ref|XP_003592159.1| Topoisomerase 1-associated factor [Medicago truncatula]
            gi|355481207|gb|AES62410.1| Topoisomerase 1-associated
            factor [Medicago truncatula]
          Length = 1335

 Score =  283 bits (724), Expect = 7e-74
 Identities = 184/452 (40%), Positives = 253/452 (55%), Gaps = 23/452 (5%)
 Frame = +3

Query: 3    RRMLRKMKTNPLIFVEILFWKTRKECHYINCESMLTDLTKMKKDYNKMGRTSFDGVDGSF 182
            R+ML+KMK  PL+FVE+LFWKTR+ECHYIN E +L +L  +K +      T  DG  GS 
Sbjct: 807  RKMLKKMKKQPLLFVEVLFWKTRRECHYINAEYLLDELGHLKNETKNWNDTQGDGEIGSS 866

Query: 183  EGKGWNRRSIADALGDDEADVMLFHETN-DHNVEKPKSSVGDVEEANKGVTSMPDEGSNG 359
             GK W RRS+ADALGDDEADV++ H++   +N EK    + D+E       S  D   N 
Sbjct: 867  PGKPWTRRSLADALGDDEADVVISHDSRYQNNGEK----LDDIEGIASTSGSKNDRDDNN 922

Query: 360  KETMIDERY----KKMKVLGSDNELCNQLKDLYEKYKDKENCIHLIVEEL--DAKISYRE 521
             E  +++ Y    ++ K L  D EL  Q+KDLYEK+KD  NC   I E L  D KIS  +
Sbjct: 923  GEPWLEDEYQTAPRRKKKLILDAELEIQIKDLYEKFKDDRNCSRRIAEVLDPDGKISTAQ 982

Query: 522  VSKKLKQLGFSFPQKRRLLKTGASNQTGEAA------NGFGNEITHPNLSGMHENSSVRR 683
            +S KLK+LG +   ++   K G +++T   +       G    + H ++ G    S + +
Sbjct: 983  ISSKLKKLGLTIASRK---KKGDADETFSTSPNQLEGGGLAGGVNHTSVEG----SLLIQ 1035

Query: 684  PMHARKRVSAFSEDQESKIKGLFEQFKYHKRCSFMIASALDADGSFTVAQVSRKLKQLGL 863
                RKRVSAF+EDQE++IK LFE FK H+RCS+MIA+ALD DG FT AQVSRKLKQLGL
Sbjct: 1036 HRQKRKRVSAFNEDQEARIKVLFEDFKDHRRCSYMIANALDKDGKFTPAQVSRKLKQLGL 1095

Query: 864  RAPQKQMLNSHLQLRDEDPDDNSDEGI--DDTETLSSLSKRTKTKHNTSSVTTIPEQKLA 1037
              PQK     + Q + ED  D S++ +   D +TL SL KR K K+   S   + EQ   
Sbjct: 1096 CVPQKSFRGKNHQ-KGEDLMDGSNDRMHESDEDTLISLVKRKKGKNRKESSEQLREQTNE 1154

Query: 1038 EKFTHEVSDDEPLSSLVGNKTKQKRGNVAERQDQKKYRKVSRXXXXXXXXXXIVGHKHEQ 1217
            +K + + SDDE L+S++  K K +  +  + Q+Q    K+S+          I+  K + 
Sbjct: 1155 DKLSKDDSDDEMLASILKKKGKNRNISSEQLQEQTGEDKLSKDDSDDEILGSILKKKVKN 1214

Query: 1218 PTE--------TANDALHIDDLEAAVKDSSKK 1289
              E        T  D L  DDL+  +  S  K
Sbjct: 1215 RKESRKQLQEQTGEDKLSKDDLDEEILGSILK 1246


>ref|XP_002331831.1| predicted protein [Populus trichocarpa] gi|222875069|gb|EEF12200.1|
            predicted protein [Populus trichocarpa]
          Length = 1341

 Score =  260 bits (665), Expect = 5e-67
 Identities = 169/387 (43%), Positives = 221/387 (57%), Gaps = 7/387 (1%)
 Frame = +3

Query: 3    RRMLRKMKTNPLIFVEILFWKTRKECHYINCESMLTDLTKMKKDYNKMGRTSFDGVDGSF 182
            RRMLRKMK  PL+FVE+LFWK+RKECHYIN E ML +L  +KK+    G  S +   GS 
Sbjct: 830  RRMLRKMKNQPLLFVEVLFWKSRKECHYINAEYMLHELGHLKKESAGWGNASANKDIGSS 889

Query: 183  EGKGWNRRSIADALGDDEADVMLFHETNDHNVEKPKSSVGDVEEANKGVTSMPDEGSNGK 362
            +GK W  RSIADALG+DEADV++ HE    N        GD  E                
Sbjct: 890  QGKRWAPRSIADALGEDEADVVIPHELGYQN-------GGDAAE---------------H 927

Query: 363  ETMIDERYKKMKVLGSDNELCNQLKDLYEKYKDKENCIHLIVEEL--DAKISYREVSKKL 536
            E++   R K+  VL  + E+  ++KDLYEK+KD ENC HLI E L  D ++S  +V  KL
Sbjct: 928  ESVSVPRRKRRFVLTDEMEM--KIKDLYEKFKDDENCSHLIAESLDPDGQVSRAQVINKL 985

Query: 537  KQLGFSFPQKRRLLKTGASNQTGEAANGFGNEITHPNLSGMHENSSVRRP---MHARKRV 707
            KQLG     K+R    G    T     G   EI     S +H +  +      +  RKRV
Sbjct: 986  KQLGLKVASKKRKRSVGRPFSTNPDQLGENGEIIEKE-SNLHNSIDLEGSLPRLSTRKRV 1044

Query: 708  SAFSEDQESKIKGLFEQFKYHKRCSFMIASALDADGSFTVAQVSRKLKQLGLRAPQKQML 887
             AF++DQE  IK LFEQFK H+RCS+MIA+A+ +D SFT AQVSRKLKQLGLRAP+++  
Sbjct: 1045 RAFNKDQEEMIKALFEQFKDHRRCSYMIANAMGSDNSFTAAQVSRKLKQLGLRAPRQKQS 1104

Query: 888  NSHLQLRDEDPDDNSDEGID-DTETLSSLSKRTKTKHNTSSV-TTIPEQKLAEKFTHEVS 1061
             + + LRDE+ +  S  G D D ETL SL  R+K K +       +P Q +  + +   S
Sbjct: 1105 ETDMHLRDEELNSFSVGGQDSDDETLLSLKNRSKNKDDGGLFGDELPSQNIEGESSD--S 1162

Query: 1062 DDEPLSSLVGNKTKQKRGNVAERQDQK 1142
            DDE L S++  K       VA +Q+ +
Sbjct: 1163 DDELLCSILKPKNNAGDRLVAGKQNNE 1189


>ref|XP_003555400.1| PREDICTED: protein timeless homolog [Glycine max]
          Length = 1171

 Score =  244 bits (623), Expect = 4e-62
 Identities = 155/373 (41%), Positives = 213/373 (57%), Gaps = 11/373 (2%)
 Frame = +3

Query: 3    RRMLRKMKTNPLIFVEILFWKTRKECHYINCESMLTDLTKMKKDYNKMGRTSFDGVDGSF 182
            R+ML+KMK  PL+FVE+LFWKTR+ECHYIN E +L++L  +KK+      T  D   GS 
Sbjct: 736  RKMLKKMKKQPLLFVELLFWKTRRECHYINAEYLLSELGHLKKESANWNNTQGDEEIGSS 795

Query: 183  EGKGWNRRSIADALGDDEADVMLFHETNDHNVEKPKSSVGDVEEANKGVTSMPDEGSNGK 362
              K W RRSIADALG+DEADV++ H++   N                G   M D      
Sbjct: 796  PAKVWTRRSIADALGEDEADVVITHDSGYQN----------------GEQLMED------ 833

Query: 363  ETMIDERYKKMKVLGSDNELCNQLKDLYEKYKDKENCIHLIVEEL--DAKISYREVSKKL 536
            E+ I  R +K  VL  D +L  Q+KDL+EK+KD ++C H I E L  D KIS  ++S  L
Sbjct: 834  ESQIAPRRRKKLVL--DGDLERQIKDLHEKFKDDQHCSHRIAEVLDPDGKISPAQISNML 891

Query: 537  KQLGFSFPQKRRLLKTGASNQTGEAANGFGNEITHPNLSGMHENSSVR-------RPMHA 695
            K+LG +   +R++    A      +     N++    ++G   + SV        + +  
Sbjct: 892  KRLGLAVAPRRKMCDADAEGPLSTSP----NQLDSDKITGATNHKSVNLEGSLLVQHLQK 947

Query: 696  RKRVSAFSEDQESKIKGLFEQFKYHKRCSFMIASALDADGSFTVAQVSRKLKQLGLRAPQ 875
            +KRV AF++DQE+ IK L+EQFK  +RCS+MIA+ALD DG FT AQVSRKLKQLGL  P 
Sbjct: 948  KKRVQAFNKDQEALIKVLYEQFKDQRRCSYMIANALDKDGKFTTAQVSRKLKQLGLSLPL 1007

Query: 876  KQMLNSHLQLRDEDPDDNSDEGID--DTETLSSLSKRTKTKHNTSSVTTIPEQKLAEKFT 1049
            K      +  +  D  D S+E +D  D ETL SL KR K + +  S   +  Q   +K +
Sbjct: 1008 KSS-GGKMHPKGADLMDRSNERMDESDDETLVSLVKRKKMESDKLSRGQLHGQTSEDKLS 1066

Query: 1050 HEVSDDEPLSSLV 1088
             + SDDE LSS++
Sbjct: 1067 KDDSDDEMLSSVL 1079