BLASTX nr result
ID: Bupleurum21_contig00008272
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00008272
(2336 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002267490.2| PREDICTED: probable ubiquitin-conjugating en... 482 e-133
ref|XP_002329823.1| predicted protein [Populus trichocarpa] gi|2... 461 e-127
gb|ACB12194.1| PHO2 [Nicotiana benthamiana] 436 e-119
ref|XP_002305135.1| predicted protein [Populus trichocarpa] gi|2... 429 e-117
ref|XP_002519066.1| ubiquitin conjugating enzyme, putative [Rici... 425 e-116
>ref|XP_002267490.2| PREDICTED: probable ubiquitin-conjugating enzyme E2 24-like [Vitis
vinifera] gi|297735992|emb|CBI23966.3| unnamed protein
product [Vitis vinifera]
Length = 944
Score = 482 bits (1241), Expect = e-133
Identities = 249/447 (55%), Positives = 318/447 (71%), Gaps = 12/447 (2%)
Frame = +1
Query: 1024 MYGGQACSILSNLEETIEKIDDFLSFERGFVHGDIVSSAEDPSGQMGKVINVDMIVDLEN 1203
MYGG+A SILS+LEETI KIDDFLSFERGF+HGDIV S DPSGQMG+V++VDM+VDLEN
Sbjct: 28 MYGGKAQSILSSLEETIGKIDDFLSFERGFIHGDIVCSVADPSGQMGRVVDVDMLVDLEN 87
Query: 1204 VYGTKTRHISSNKLGKIRSISVGDYVVGGPWIGKVENIVDHVTILFDDGTKCEYNTMGPE 1383
VYG + ++S KL KIRSIS+GDYVV GPW+G+V +VD VTILFDDG KCE +
Sbjct: 88 VYGKVIKDVNSKKLLKIRSISIGDYVVLGPWLGRVSKVVDSVTILFDDGAKCEVTATDHK 147
Query: 1384 KLIPISPDFLDDPQYPYYPGQRVRVELSSIDKSSRWLCGPRKEKRDEGTICAVDAGTVYV 1563
K+ PISPD L+DPQY YYPGQRV+V LS++ KS+RWLCG KE R +GT+CAV+AG V V
Sbjct: 148 KIFPISPDLLEDPQYQYYPGQRVQVRLSTLSKSARWLCGAWKENRTDGTVCAVEAGLVSV 207
Query: 1564 DWLACVLVGP-KKLAAPLRLQNSKNLTLLSCSSHSSWQLGDWCTLPCTDDMGVIEHLYPY 1740
DWLA LVG L AP Q+SK LTLLSC SH++WQLGDWC LP +D GV+E
Sbjct: 208 DWLASALVGSGLSLPAPPCWQDSKKLTLLSCFSHANWQLGDWCMLPVSDRKGVMEKNCLS 267
Query: 1741 SSNG----GLPQSERVYER-SFAPKLQDLYVIVKRMTKADVLWQDGSKSYGLDSHALFPV 1905
S G + ER ++R + + + ++++V+VK TK DV WQDGS S GLD +LFP+
Sbjct: 268 VSTCEHILGHMKLERGFKRMNLSFERENIFVMVKIKTKVDVQWQDGSCSVGLDPQSLFPI 327
Query: 1906 NIIDAHDFWPDQYVQNS---DDPHNTDNQRWGVVRSVDAKERTVRVRWETSKTNQLTDLE 2076
NI+DAH+FWP+Q+V DDP+ + +QRWGVV VDAKER V+V+W+ N+ DLE
Sbjct: 328 NIVDAHEFWPEQFVLEKGTCDDPNVSSSQRWGVVEVVDAKERIVKVKWKNFALNEGNDLE 387
Query: 2077 AFGKEEIVSAYQLVEHPDLSYSIGDAVFKMHKGNLSDIATGKNYNDDGTGKGFLNETADI 2256
EE VSAY+LVEHPD SY +GD VF++ + L D A G+N+N++ + + + +
Sbjct: 388 EGLMEETVSAYELVEHPDYSYCLGDFVFRLERNQLVDQADGQNFNNNAIAEMGMGKEIPL 447
Query: 2257 CHE---EDQNDMSSISYLSQIGIIVGF 2328
E +DQN+ S Y S IG +VGF
Sbjct: 448 KGETCSKDQNEYSDKYYSSHIGNVVGF 474
>ref|XP_002329823.1| predicted protein [Populus trichocarpa] gi|222870885|gb|EEF08016.1|
predicted protein [Populus trichocarpa]
Length = 924
Score = 461 bits (1187), Expect = e-127
Identities = 241/445 (54%), Positives = 305/445 (68%), Gaps = 8/445 (1%)
Frame = +1
Query: 1024 MYGGQACSILSNLEETIEKIDDFLSFERGFVHGDIVSSAEDPSGQMGKVINVDMIVDLEN 1203
+YGG+A SI S+LEE+I KIDDFLSFERGFVHGDIVSS DPSGQMG+V+NV+M+V+LEN
Sbjct: 29 LYGGRASSIFSSLEESIGKIDDFLSFERGFVHGDIVSSVTDPSGQMGRVVNVNMLVNLEN 88
Query: 1204 VYGTKTRHISSNKLGKIRSISVGDYVVGGPWIGKVENIVDHVTILFDDGTKCEYNTMGPE 1383
+G + + S KL KIRSISVGDYVV GPWIG+V+ +VD+VT++FDDGT CE + E
Sbjct: 89 RHGKIIKDVDSKKLLKIRSISVGDYVVHGPWIGRVDKVVDNVTVVFDDGTSCEVTAVDQE 148
Query: 1384 KLIPISPDFLDDPQYPYYPGQRVRVELSSIDKSSRWLCGPRKEKRDEGTICAVDAGTVYV 1563
KL+PIS + L+DP YPYYPGQRVR+ LS++ KS+RWLCG KE +D GT+ AV AG VYV
Sbjct: 149 KLLPISSNILEDPTYPYYPGQRVRIRLSAVSKSARWLCGVWKENQDVGTVSAVKAGLVYV 208
Query: 1564 DWLACVLVGPKKLAAPLRLQNSKNLTLLSCSSHSSWQLGDWCTLPCTDDMGVIEHLYPYS 1743
DWLAC LV L AP RLQ+++NLTLLSC H +WQLGDWC LP D G+ ++ +
Sbjct: 209 DWLACALV-DLSLPAPQRLQDARNLTLLSCFLHENWQLGDWCMLPLADCKGMNGQIFFDA 267
Query: 1744 SNGGLPQSERVYERSFAPK-----LQDLYVIVKRMTKADVLWQDGSKSYGLDSHALFPVN 1908
S + + +R F + QD++VIVK T DV+WQDG S GLDS +L PVN
Sbjct: 268 SIIKIIKEDRRIGHGFKGQNPCLNFQDIFVIVKTKTIVDVVWQDGGCSQGLDSQSLLPVN 327
Query: 1909 IIDAHDFWPDQYVQNS---DDPHNTDNQRWGVVRSVDAKERTVRVRWETSKTNQLTDLEA 2079
I++AHDFWP Q+V DDPH + NQ+WGVV VDAKERTV V+W+ NQ+ ++ +
Sbjct: 328 IVNAHDFWPGQFVLEKGACDDPHVSGNQKWGVVNCVDAKERTVMVKWKFIGVNQVNNVGS 387
Query: 2080 FGKEEIVSAYQLVEHPDLSYSIGDAVFKMHKGNLSDIATGKNYNDDGTGKGFLNETADIC 2259
EE VSAY+LVEHPD SYS GD VFK NL K++ + TG AD
Sbjct: 388 GQIEETVSAYELVEHPDYSYSYGDIVFK----NLDQ--ANKDHVNRETGM-----NADAP 436
Query: 2260 HEEDQNDMSSISYLSQIGIIVGFDN 2334
E + + YL IG + GF++
Sbjct: 437 LEGSDHGKDQVDYLCCIGYVTGFED 461
>gb|ACB12194.1| PHO2 [Nicotiana benthamiana]
Length = 921
Score = 436 bits (1121), Expect = e-119
Identities = 222/444 (50%), Positives = 296/444 (66%), Gaps = 7/444 (1%)
Frame = +1
Query: 1024 MYGGQACSILSNLEETIEKIDDFLSFERGFVHGDIVSSAEDPSGQMGKVINVDMIVDLEN 1203
+YGG ACSILS+LEE+I IDDFLSFER F++GDIV + DPSGQ+GKVINV+MIVDLEN
Sbjct: 27 LYGGHACSILSSLEESIGNIDDFLSFERVFMYGDIVCAVNDPSGQIGKVINVEMIVDLEN 86
Query: 1204 VYGTKTRHISSNKLGKIRSISVGDYVVGGPWIGKVENIVDHVTILFDDGTKCEYNTMGPE 1383
+YG+K + ++S L KIR ISVGDYVV GPW+GKVE IVD V +LFDDG K E+ G E
Sbjct: 87 IYGSKIQDVNSKDLVKIRPISVGDYVVMGPWLGKVEKIVDKVKVLFDDGAKSEFAAEGSE 146
Query: 1384 KLIPISPDFLDDPQYPYYPGQRVRVELSSIDKSSRWLCGPRKEKRDEGTICAVDAGTVYV 1563
L PISPD ++DPQYP+YPGQRV+V+ ++ S+ WLCG + KRD+GTI +V+AG +YV
Sbjct: 147 ILTPISPDLVEDPQYPFYPGQRVQVQSAAASGSTNWLCGVKSGKRDQGTIYSVEAGVMYV 206
Query: 1564 DWLACVLVGPKKLAAPLRLQNSKNLTLLSCSSHSSWQLGDWCTLPCTDDMGV----IEHL 1731
DW+ C +G +K+ +P L +S+ LTLLSC SH+ WQLGD C LP D I+
Sbjct: 207 DWIGCGSLGCEKVLSPPNLLDSEKLTLLSCFSHAKWQLGDCCLLPVADSKNFLQQSIQRS 266
Query: 1732 YPYSSNGGLPQSERVYERSFAPKLQDLYVIVKRMTKADVLWQDGSKSYGLDSHALFPVNI 1911
PY + Q + + + + + + VI+K TK DV WQDGS + GLDS ++FPVN+
Sbjct: 267 PPYGAMKEDMQLNKASQSNRSSTVPQVAVILKTRTKVDVSWQDGSVTTGLDSDSVFPVNV 326
Query: 1912 IDAHDFWPDQYVQNS---DDPHNTDNQRWGVVRSVDAKERTVRVRWETSKTNQLTDLEAF 2082
+DAH+FWP+Q+V DD +RWGVVR VDAKERTV+V+W T N+
Sbjct: 327 VDAHEFWPEQFVLEKGYCDDSSVPSPKRWGVVRCVDAKERTVKVKWTTFSLNEPNKFGVE 386
Query: 2083 GKEEIVSAYQLVEHPDLSYSIGDAVFKMHKGNLSDIATGKNYNDDGTGKGFLNETADICH 2262
EEIVSAY+L++HPD SY GDAV K + + + Y++ ++ +I
Sbjct: 387 QTEEIVSAYELMDHPDYSYCFGDAVCKFCEDQVFSLDGKSMYSE----MDINSQLKNIDK 442
Query: 2263 EEDQNDMSSISYLSQIGIIVGFDN 2334
D +D + +L IGIIVGF++
Sbjct: 443 RRDNSDFTGYDHLPCIGIIVGFED 466
>ref|XP_002305135.1| predicted protein [Populus trichocarpa] gi|222848099|gb|EEE85646.1|
predicted protein [Populus trichocarpa]
Length = 925
Score = 429 bits (1104), Expect = e-117
Identities = 226/446 (50%), Positives = 299/446 (67%), Gaps = 9/446 (2%)
Frame = +1
Query: 1024 MYGGQACSILSNLEETIEKIDDFLSFERGFVHGDIVSSAEDPSGQMGKVINVDMIVDLEN 1203
+Y G+A SILS+LEE+ KIDDFLSFERGF+HGD+V SA DPSGQMG+V+NV+M+V+LE+
Sbjct: 29 LYCGRASSILSSLEESKGKIDDFLSFERGFIHGDVVCSATDPSGQMGRVVNVNMLVNLES 88
Query: 1204 VYGTKTRHISSNKLGKIRSISVGDYVVGGPWIGKVENIVDHVTILFDDGTKCEYNTMGPE 1383
+G + + S KL KIRSISVGDYVV GPWIG+V+ +++ VT++FDDGT CE + E
Sbjct: 89 SHGRIIKDVDSKKLLKIRSISVGDYVVHGPWIGRVDEVIESVTVIFDDGTSCEVTAVDQE 148
Query: 1384 KLIPISPDFLDDPQYPYYPGQRVRVELSSIDKSSRWLCGPRKEKRDEGTICAVDAGTVYV 1563
KL ISP+ L+D +PYYPGQRV++ LS++ KS+ WLCG E +D GT+ AV AG V V
Sbjct: 149 KLSRISPNMLEDSMHPYYPGQRVQIRLSALSKSATWLCGAWNENQDVGTVSAVKAGLVSV 208
Query: 1564 DWLACVLVGPK-KLAAPLRLQNSKNLTLLSCSSHSSWQLGDWCTLPCTDDMGVIEHLYPY 1740
DWLAC LVG L AP RLQ++K L LLSC H +WQLGDWC LP D G E +
Sbjct: 209 DWLACALVGSDLSLPAPQRLQDAKKLNLLSCFLHENWQLGDWCMLPLADCTGGKERILFD 268
Query: 1741 SSNGGLPQSERVYERSF-----APKLQDLYVIVKRMTKADVLWQDGSKSYGLDSHALFPV 1905
+S + + +R + F +P QD++VIVK T +V+WQDGS S GLDS +L PV
Sbjct: 269 ASIIEIIEKDRKRGQGFTGLNPSPIFQDIFVIVKTKTIVNVVWQDGSCSQGLDSKSLLPV 328
Query: 1906 NIIDAHDFWPDQYVQNS---DDPHNTDNQRWGVVRSVDAKERTVRVRWETSKTNQLTDLE 2076
NI++AHDFWP Q+V +DP+ + NQ+WG V VDAKE+ V+V+W++ NQ +
Sbjct: 329 NIVNAHDFWPGQFVLGKGACEDPYVSGNQKWGFVNCVDAKEQIVKVKWKSFGVNQADIVG 388
Query: 2077 AFGKEEIVSAYQLVEHPDLSYSIGDAVFKMHKGNLSDIATGKNYNDDGTGKGFLNETADI 2256
+ EE VSAY+LVEHP+ S+S GD VFK NL K++ + TG G
Sbjct: 389 SDQIEETVSAYELVEHPEYSFSYGDIVFK----NLDQ--ANKDHLNRDTGMGADAALEGC 442
Query: 2257 CHEEDQNDMSSISYLSQIGIIVGFDN 2334
H +DQ + YLS IG + GF++
Sbjct: 443 AHGKDQ-----VDYLSCIGCVTGFED 463
>ref|XP_002519066.1| ubiquitin conjugating enzyme, putative [Ricinus communis]
gi|223541729|gb|EEF43277.1| ubiquitin conjugating enzyme,
putative [Ricinus communis]
Length = 925
Score = 425 bits (1092), Expect = e-116
Identities = 230/448 (51%), Positives = 305/448 (68%), Gaps = 13/448 (2%)
Frame = +1
Query: 1024 MYGGQACSILSNLEETIEKIDDFLSFERGFVHGDIVSSAEDPSGQMGKVINVDMIVDLEN 1203
+YGG A ILS+LEE+I KIDDFLSFERGFV G++V S +PSGQMG+V+NV M+VDLEN
Sbjct: 23 LYGGHANCILSSLEESIGKIDDFLSFERGFVRGEVVCSVANPSGQMGRVVNVKMVVDLEN 82
Query: 1204 VYGTKTRHISSNKLGKIRSISVGDYVVGGPWIGKVENIVDHVTILFDDGTKCEYNTMGPE 1383
V+G + + S +L KI S+SVGDYVV GPWIG+V+ +V +VTI+FDDG+KCE E
Sbjct: 83 VHGKIIKAVDSKELLKICSMSVGDYVVNGPWIGRVDKVVHNVTIIFDDGSKCEVIAADKE 142
Query: 1384 KLIPISPDFLDDPQYPYYPGQRVRVELSSIDKSSRWLCGPRKEKRDEGTICAVDAGTVYV 1563
KL+P+SP+ L+D YPYYPGQRV+V L ++ K +R LCG KE +D GT+ +V+AG ++V
Sbjct: 143 KLLPVSPNILEDSTYPYYPGQRVQVRLPAVSK-TRSLCGAWKENQDVGTVSSVNAGLMFV 201
Query: 1564 DWLACVLVG-PKKLAAPLRLQNSKNLTLLSCSSHSSWQLGDWCTLPCTDDMGVIEH-LYP 1737
DWLAC LVG L AP LQ+ K+LTLL CSS+ WQLGDWC LP D GV + LY
Sbjct: 202 DWLACALVGCDMSLPAPRHLQDVKDLTLLPCSSYEHWQLGDWCMLPFADFKGVKKQMLYD 261
Query: 1738 YSSNGGLPQSERV---YERSFAPKLQDLYVIVKRMTKADVLWQDGSKSYGLDSHALFPVN 1908
S+ + +++++ ++R L++++VIVK T DVLWQDGS S LDSH+L PVN
Sbjct: 262 ASTLELIKENDKMGKGFKRQDHSNLEEIFVIVKIKTIVDVLWQDGSCSLELDSHSLLPVN 321
Query: 1909 IIDAHDFWPDQYVQNS---DDPHNTDNQRWGVVRSVDAKERTVRVRWETSKTNQLTDL-- 2073
+++AH+FWP QYV DDP+ DN++WGVV +VDAKERTV+V+W + NQ D+
Sbjct: 322 VVNAHEFWPGQYVVEKGACDDPNVPDNRKWGVVSAVDAKERTVKVKWRLTVANQANDVGS 381
Query: 2074 EAFGKEEIVSAYQLVEHPDLSYSIGDAVFKMHKGNLSDIATGKNYNDDGTGKGFLNETAD 2253
+ E VSAY+LVE+PD SY GD VFK + +D+ K G+ + ET
Sbjct: 382 NLVSQGETVSAYELVEYPDFSYCYGDIVFK--TVDQADMHRLK-------GETSMGETVA 432
Query: 2254 ICHEE---DQNDMSSISYLSQIGIIVGF 2328
I +E DQ+D YLS IG + GF
Sbjct: 433 IEGKECGKDQSDYPCDGYLSCIGYVSGF 460