BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00008074
         (340 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABG76785.1| ribosome-inactivating protein [Panax ginseng]           99   3e-19
gb|AFK73425.1| type 2 ribosomal inactivating protein [Jatropha c...    82   6e-14
gb|ADF45510.1| type 2 ribosome-inactivating protein precursor [C...    80   1e-13
gb|ACV60361.2| putative type 2 ribosome-inactivating protein [Ca...    80   1e-13
gb|ADF45512.1| type 2 ribosome-inactivating protein precursor [C...    80   2e-13

>gb|ABG76785.1| ribosome-inactivating protein [Panax ginseng]
          Length = 443

 Score = 99.4 bits (246), Expect = 3e-19
 Identities = 60/142 (42%), Positives = 76/142 (53%), Gaps = 30/142 (21%)
 Frame = -2

Query: 336 ITSDEDNAVFFPDATVIAFEDSWDKLSESIQSSVAGVLFPAFVY---SDASEPVTSITPT 166
           I +D     F P  T+I+ E++WD LS SIQ SV G   P+       D S  V+S+TP 
Sbjct: 222 IRADGSRQNFRPTRTMISLENNWDTLSTSIQQSVEGRFTPSVELVNREDQSVLVSSVTPG 281

Query: 165 FGLNIALLRYVSSTGN---------------------------IPEPTVRIIGRNGLCAD 67
             LNIA++ +V +  N                           IPEPT+RI GRNGLCAD
Sbjct: 282 LILNIAVMIFVCNNPNPNQLISPMRHVNVDHVMGINDDHICSIIPEPTIRISGRNGLCAD 341

Query: 66  VQDAFYDDGNPIILWPCKSNSD 1
           V+D  + DGNPIIL+PCKSN D
Sbjct: 342 VKDRSFHDGNPIILFPCKSNED 363


>gb|AFK73425.1| type 2 ribosomal inactivating protein [Jatropha curcas]
          Length = 551

 Score = 81.6 bits (200), Expect = 6e-14
 Identities = 49/129 (37%), Positives = 63/129 (48%), Gaps = 22/129 (17%)
 Frame = -2

Query: 330 SDEDNAVFFPDATVIAFEDSWDKLSESIQSSVAGVLFPAFVYSDASEPVT---------- 181
           S E    F PD T+ + E++W+ LS ++Q S  GV          SEPV           
Sbjct: 209 STEKGQRFRPDGTIYSLENNWEALSTAVQESRGGVF---------SEPVQLQRLNVKFNL 259

Query: 180 -SITPTFGLNIALLRYVSSTGNIP-----------EPTVRIIGRNGLCADVQDAFYDDGN 37
            S+T     N+ L+ ++S   N             EPTVRI+GRNGLC DV D  Y DGN
Sbjct: 260 DSVTRELVANLGLMLFISCKDNSTSSGGGGCATDFEPTVRILGRNGLCLDVSDEIYSDGN 319

Query: 36  PIILWPCKS 10
            I +WPCKS
Sbjct: 320 KIHMWPCKS 328


>gb|ADF45510.1| type 2 ribosome-inactivating protein precursor [Camellia sinensis]
           gi|294861498|gb|ADF45511.1| type 2 ribosome-inactivating
           protein precursor [Camellia sinensis]
          Length = 570

 Score = 80.5 bits (197), Expect = 1e-13
 Identities = 48/134 (35%), Positives = 67/134 (50%), Gaps = 31/134 (23%)
 Frame = -2

Query: 309 FFPDATVIAFEDSWDKLSESIQSSVAGVLF-------PAFVYSDASEPVTSITPTFGLNI 151
           F PDA +++ E++W  LS +IQ S  GV         P + + +      SI      N+
Sbjct: 223 FRPDAGMLSLENNWGALSYAIQQSNQGVFSSPVQLQRPDYTFFNVDSATLSIVR----NL 278

Query: 150 ALLRYVSSTGNI------------------------PEPTVRIIGRNGLCADVQDAFYDD 43
           AL+ +V +  +                         PEPT+RI GRNGLC DV+D  Y+D
Sbjct: 279 ALMLFVCNINHASSQFSPLIRSVVAPVDDDDDTCAEPEPTIRISGRNGLCVDVRDGQYND 338

Query: 42  GNPIILWPCKSNSD 1
           GNPI LWPC+SN+D
Sbjct: 339 GNPIQLWPCESNAD 352


>gb|ACV60361.2| putative type 2 ribosome-inactivating protein [Camellia sinensis]
           gi|294861494|gb|ADF45509.1| type 2 ribosome-inactivating
           protein precursor [Camellia sinensis]
          Length = 570

 Score = 80.5 bits (197), Expect = 1e-13
 Identities = 48/134 (35%), Positives = 67/134 (50%), Gaps = 31/134 (23%)
 Frame = -2

Query: 309 FFPDATVIAFEDSWDKLSESIQSSVAGVLF-------PAFVYSDASEPVTSITPTFGLNI 151
           F PDA +++ E++W  LS +IQ S  GV         P + + +      SI      N+
Sbjct: 223 FRPDAGMLSLENNWGALSYAIQQSNQGVFSSPVQLQRPDYTFFNVDSATLSIVR----NL 278

Query: 150 ALLRYVSSTGNI------------------------PEPTVRIIGRNGLCADVQDAFYDD 43
           AL+ +V +  +                         PEPT+RI GRNGLC DV+D  Y+D
Sbjct: 279 ALMLFVCNINHASSQFSPLIRSVVAPVDDDDDTCAEPEPTIRISGRNGLCVDVRDGQYND 338

Query: 42  GNPIILWPCKSNSD 1
           GNPI LWPC+SN+D
Sbjct: 339 GNPIQLWPCESNTD 352


>gb|ADF45512.1| type 2 ribosome-inactivating protein precursor [Camellia sinensis]
          Length = 570

 Score = 80.1 bits (196), Expect = 2e-13
 Identities = 49/130 (37%), Positives = 65/130 (50%), Gaps = 27/130 (20%)
 Frame = -2

Query: 309 FFPDATVIAFEDSWDKLSESIQSSVAGVLF-------PAFVYSDASEPVTSITPTFGLNI 151
           F PDA +++ E++W  LS +IQ S  GV         P + + +      SI     L +
Sbjct: 223 FRPDAGMLSLENNWGALSYAIQQSNQGVFSSPVQLQRPDYTFFNVDSATLSIVRNLALML 282

Query: 150 -------------ALLRYV-------SSTGNIPEPTVRIIGRNGLCADVQDAFYDDGNPI 31
                         L+R V         T   PEPT+RI GRNGLC DV+D  Y+DGNPI
Sbjct: 283 FVCNINQASSQFSPLIRSVVAPVDDDDDTCAEPEPTIRISGRNGLCVDVRDGQYNDGNPI 342

Query: 30  ILWPCKSNSD 1
            LWPC+SN+D
Sbjct: 343 QLWPCESNTD 352