BLASTX nr result
ID: Bupleurum21_contig00007933
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00007933
(1702 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAN65790.1| hypothetical protein VITISV_042049 [Vitis vinifera] 520 0.0
ref|XP_002270736.1| PREDICTED: cytochrome P450 77A2 [Vitis vinif... 520 0.0
gb|ADJ57933.1| cytochrome P450 [Solanum torvum] 505 0.0
sp|P37123.1|C77A1_SOLME RecName: Full=Cytochrome P450 77A1; AltN... 489 0.0
sp|P37124.1|C77A2_SOLME RecName: Full=Cytochrome P450 77A2; AltN... 495 e-180
>emb|CAN65790.1| hypothetical protein VITISV_042049 [Vitis vinifera]
Length = 515
Score = 520 bits (1338), Expect(2) = 0.0
Identities = 253/391 (64%), Positives = 311/391 (79%), Gaps = 1/391 (0%)
Frame = +1
Query: 49 TTMEYHILVFVSIALFISSLF-FLTRXXXXXXXXXXXXXXXXXXIGNLFQVVGSGMPFFM 225
T Y+ + F ++A IS L L+R +GNLFQ SG FF
Sbjct: 11 TYSPYYHIFFTALAFLISGLIVLLSRKTKSKKLNLPPGPPGWPIVGNLFQFARSGKQFFQ 70
Query: 226 YIRDVLIPKYGPILTIHMGTRTTIIVSDAELLREALIEKGQIFASRPMENPTRIVFSCNK 405
Y+R+ L PKYGPI T+ MG RT II+S AEL EALIEKGQ FASRP ENPTR VFSCNK
Sbjct: 71 YVRE-LRPKYGPIFTLKMGNRTMIIISSAELAHEALIEKGQSFASRPRENPTRTVFSCNK 129
Query: 406 FAVNSSLYGPVWRSLRRNMVQNMLSASRLKGFRDVREKGMDKLIEKIKEEADANDGTVRV 585
F VN+++YGPVWRSLRRNMVQNMLSAS+++ F ++R+ MDKLI++++ EA+ANDG V V
Sbjct: 130 FTVNAAVYGPVWRSLRRNMVQNMLSASKIREFSNLRDVSMDKLIDRLRSEAEANDGAVWV 189
Query: 586 LKNARFAFFCVLLTMCFGIELEEEMIEKVDDMMKTVLITVDPRIDDFLPLLSPFFYRQRK 765
LKNARFA FC+LL+MCFG+E++EE IE +DD+MKTVLIT+DPR+DD+LPLLSPFF +QRK
Sbjct: 190 LKNARFAVFCILLSMCFGVEMDEETIEVMDDLMKTVLITLDPRLDDYLPLLSPFFSKQRK 249
Query: 766 KALQVRQQQIDTIVPLIKRRRLALENPGSDKTATSFSYLDTLFELEVEGQKVNPSNAQLV 945
A +VR++QI T+VP I+RRR ALENPGSDKTA SFSYLDT+F+L++EG+K +P+N +LV
Sbjct: 250 AATEVRKRQIKTVVPFIERRRAALENPGSDKTAASFSYLDTIFDLKIEGRKSSPTNPELV 309
Query: 946 TLCSEFLNGGTDTTGTGIEWAMARIIENPHIQSRLYDEIKSVAGDKKVDEHDMQKMPYLN 1125
TLCSEFLNGGTDTTGT +EWA+AR+IENP IQS+LY+EIK+ GD+KV E DM+KMPYLN
Sbjct: 310 TLCSEFLNGGTDTTGTAVEWAIARMIENPEIQSKLYEEIKTTVGDRKVQEKDMEKMPYLN 369
Query: 1126 AFCKELLRRHPPTYFTLTHAVVEPTTLGGYD 1218
A KELLR+HPPTYF+LTHAV EP L GYD
Sbjct: 370 AVSKELLRKHPPTYFSLTHAVTEPAKLAGYD 400
Score = 179 bits (453), Expect(2) = 0.0
Identities = 84/108 (77%), Positives = 94/108 (87%)
Frame = +2
Query: 1301 EFFLQGISNDPKLWPNPDKFDPDRFLTGKEDPDITGVKDVRLVPFGIGRRICPGLGMASV 1480
EFFL IS DPKLW NP+KFDPDRFL G ED DITGV V+++PFG+GRRICPGL MA+V
Sbjct: 408 EFFLPPISEDPKLWKNPEKFDPDRFLLGGEDADITGVTGVKMMPFGVGRRICPGLSMATV 467
Query: 1481 HVNLMIARMVQAFEWSAYPENSKIDFTEGLEFTVIMKNSLIAKIKPRM 1624
HVNLM+ARMVQ FEWSAYPENSKIDF+E LEFTV+MKN L AKIKPR+
Sbjct: 468 HVNLMLARMVQDFEWSAYPENSKIDFSEKLEFTVVMKNPLRAKIKPRV 515
>ref|XP_002270736.1| PREDICTED: cytochrome P450 77A2 [Vitis vinifera]
gi|296090394|emb|CBI40213.3| unnamed protein product
[Vitis vinifera]
Length = 515
Score = 520 bits (1338), Expect(2) = 0.0
Identities = 253/391 (64%), Positives = 311/391 (79%), Gaps = 1/391 (0%)
Frame = +1
Query: 49 TTMEYHILVFVSIALFISSLF-FLTRXXXXXXXXXXXXXXXXXXIGNLFQVVGSGMPFFM 225
T Y+ + F ++A IS L L+R +GNLFQ SG FF
Sbjct: 11 TYSPYYHIFFTALAFLISGLIVLLSRKTKSKKLNLPPGPPGWPIVGNLFQFARSGKQFFQ 70
Query: 226 YIRDVLIPKYGPILTIHMGTRTTIIVSDAELLREALIEKGQIFASRPMENPTRIVFSCNK 405
Y+R+ L PKYGPI T+ MG RT II+S AEL EALIEKGQ FASRP ENPTR VFSCNK
Sbjct: 71 YVRE-LRPKYGPIFTLKMGNRTMIIISSAELAHEALIEKGQSFASRPRENPTRTVFSCNK 129
Query: 406 FAVNSSLYGPVWRSLRRNMVQNMLSASRLKGFRDVREKGMDKLIEKIKEEADANDGTVRV 585
F VN+++YGPVWRSLRRNMVQNMLSAS+++ F ++R+ MDKLI++++ EA+ANDG V V
Sbjct: 130 FTVNAAVYGPVWRSLRRNMVQNMLSASKIREFSNLRDVSMDKLIDRLRSEAEANDGAVWV 189
Query: 586 LKNARFAFFCVLLTMCFGIELEEEMIEKVDDMMKTVLITVDPRIDDFLPLLSPFFYRQRK 765
LKNARFA FC+LL+MCFG+E++EE IE +DD+MKTVLIT+DPR+DD+LPLLSPFF +QRK
Sbjct: 190 LKNARFAVFCILLSMCFGVEMDEETIEVMDDLMKTVLITLDPRLDDYLPLLSPFFSKQRK 249
Query: 766 KALQVRQQQIDTIVPLIKRRRLALENPGSDKTATSFSYLDTLFELEVEGQKVNPSNAQLV 945
A +VR++QI T+VP I+RRR ALENPGSDKTA SFSYLDT+F+L++EG+K +P+N +LV
Sbjct: 250 AATEVRKRQIKTVVPFIERRRAALENPGSDKTAASFSYLDTIFDLKIEGRKSSPTNPELV 309
Query: 946 TLCSEFLNGGTDTTGTGIEWAMARIIENPHIQSRLYDEIKSVAGDKKVDEHDMQKMPYLN 1125
TLCSEFLNGGTDTTGT +EWA+AR+IENP IQS+LY+EIK+ GD+KV E DM+KMPYLN
Sbjct: 310 TLCSEFLNGGTDTTGTAVEWAIARMIENPEIQSKLYEEIKTTVGDRKVQEKDMEKMPYLN 369
Query: 1126 AFCKELLRRHPPTYFTLTHAVVEPTTLGGYD 1218
A KELLR+HPPTYF+LTHAV EP L GYD
Sbjct: 370 AVSKELLRKHPPTYFSLTHAVTEPAKLAGYD 400
Score = 177 bits (449), Expect(2) = 0.0
Identities = 83/108 (76%), Positives = 93/108 (86%)
Frame = +2
Query: 1301 EFFLQGISNDPKLWPNPDKFDPDRFLTGKEDPDITGVKDVRLVPFGIGRRICPGLGMASV 1480
EFFL IS DPKLW NP+KFDPDRF G ED DITGV V+++PFG+GRRICPGL MA+V
Sbjct: 408 EFFLPPISEDPKLWKNPEKFDPDRFFLGGEDADITGVTGVKMMPFGVGRRICPGLSMATV 467
Query: 1481 HVNLMIARMVQAFEWSAYPENSKIDFTEGLEFTVIMKNSLIAKIKPRM 1624
HVNLM+ARMVQ FEWSAYPENSKIDF+E LEFTV+MKN L AKIKPR+
Sbjct: 468 HVNLMLARMVQDFEWSAYPENSKIDFSEKLEFTVVMKNPLRAKIKPRV 515
>gb|ADJ57933.1| cytochrome P450 [Solanum torvum]
Length = 511
Score = 505 bits (1301), Expect(2) = 0.0
Identities = 247/387 (63%), Positives = 309/387 (79%), Gaps = 1/387 (0%)
Frame = +1
Query: 61 YHILVFVSIALFISSL-FFLTRXXXXXXXXXXXXXXXXXXIGNLFQVVGSGMPFFMYIRD 237
Y+ L+F +A IS++ +FL++ +GNL QV SG PFF +R+
Sbjct: 12 YYHLIFTMLAFVISTIIYFLSKKAKSKKLKLPPGPPGWPVVGNLLQVARSGKPFFQIMRE 71
Query: 238 VLIPKYGPILTIHMGTRTTIIVSDAELLREALIEKGQIFASRPMENPTRIVFSCNKFAVN 417
L KYGPI T+ MGTRT II+S+A+L+ EAL++KGQ+FASRP ENPTR VFSC+KF+VN
Sbjct: 72 -LRQKYGPIFTLRMGTRTMIILSNADLVHEALVQKGQVFASRPRENPTRSVFSCDKFSVN 130
Query: 418 SSLYGPVWRSLRRNMVQNMLSASRLKGFRDVREKGMDKLIEKIKEEADANDGTVRVLKNA 597
+++YGPVWRSLR+NMVQN LS+ RLK FR VR+ MDK+IEKI+ EADANDG V VLKNA
Sbjct: 131 AAVYGPVWRSLRKNMVQNGLSSIRLKEFRAVRKSAMDKMIEKIRAEADANDGVVWVLKNA 190
Query: 598 RFAFFCVLLTMCFGIELEEEMIEKVDDMMKTVLITVDPRIDDFLPLLSPFFYRQRKKALQ 777
RFA FC+LL MCFG+E++E+ IEK+D MMKTVLI +DPR+DD+LP+LSPFF +QRK A+
Sbjct: 191 RFAVFCILLAMCFGVEMDEKTIEKIDQMMKTVLIVLDPRLDDYLPILSPFFSKQRKHAMD 250
Query: 778 VRQQQIDTIVPLIKRRRLALENPGSDKTATSFSYLDTLFELEVEGQKVNPSNAQLVTLCS 957
VR+QQI+TIVP I++R+ LE+P DKTA SFSYLDTLF+L+VEG+ P+N +LVTLCS
Sbjct: 251 VRKQQIETIVPFIEQRKKILESPEIDKTAASFSYLDTLFDLKVEGRNSAPTNPELVTLCS 310
Query: 958 EFLNGGTDTTGTGIEWAMARIIENPHIQSRLYDEIKSVAGDKKVDEHDMQKMPYLNAFCK 1137
EFLNGGTDTT T IEWA+ RIIENP+IQSRLY+EIK+ G+ KVDE D++KMPYLNA K
Sbjct: 311 EFLNGGTDTTATAIEWAIGRIIENPNIQSRLYEEIKNTVGENKVDEKDIEKMPYLNAVVK 370
Query: 1138 ELLRRHPPTYFTLTHAVVEPTTLGGYD 1218
ELLR+HPPTY +LTHAV+EP LGGYD
Sbjct: 371 ELLRKHPPTYMSLTHAVIEPVKLGGYD 397
Score = 164 bits (414), Expect(2) = 0.0
Identities = 76/108 (70%), Positives = 92/108 (85%)
Frame = +2
Query: 1301 EFFLQGISNDPKLWPNPDKFDPDRFLTGKEDPDITGVKDVRLVPFGIGRRICPGLGMASV 1480
E FL GIS DPKLW NP+KFDPDRF GKED DITGV V+++PFG+GRRICPGL MA+V
Sbjct: 405 EIFLPGISEDPKLWSNPEKFDPDRFYLGKEDADITGVSGVKMIPFGMGRRICPGLNMATV 464
Query: 1481 HVNLMIARMVQAFEWSAYPENSKIDFTEGLEFTVIMKNSLIAKIKPRM 1624
HV+L++AR+VQ FEW A PE +++DFTE LEFTV+MKN+L AKIKPR+
Sbjct: 465 HVSLILARLVQEFEW-ADPEKARVDFTEKLEFTVVMKNTLRAKIKPRI 511
>sp|P37123.1|C77A1_SOLME RecName: Full=Cytochrome P450 77A1; AltName: Full=CYPLXXVIIA1;
AltName: Full=Cytochrome P-450EG6
gi|438243|emb|CAA50647.1| P450 hydroxylase [Solanum
melongena]
Length = 499
Score = 489 bits (1259), Expect(2) = 0.0
Identities = 245/385 (63%), Positives = 302/385 (78%), Gaps = 3/385 (0%)
Frame = +1
Query: 73 VFVSIALFISS-LFFLTRXXXXXXXXXXXXXXXXXXIGNLFQVVGSGMPFFMYIRDVLIP 249
+F + +L S +F LTR +GNLFQV GSG FF YIRD L P
Sbjct: 1 IFTAFSLLFSLFIFLLTRKPKSKTPNLPPGPPGWPIVGNLFQVAGSGKQFFEYIRD-LKP 59
Query: 250 KYGPILTIHMGTRTTIIVSDAELLREALIEKGQIFASRPMENPTRIVFSCNKFAVNSSLY 429
KYG I T+ MG+RT IIV+ AEL EALI+KGQIFASRP ENPTR +FSCNKF+VN+++Y
Sbjct: 60 KYGSIFTLKMGSRTMIIVASAELAHEALIQKGQIFASRPRENPTRTIFSCNKFSVNAAVY 119
Query: 430 GPVWRSLRRNMVQNMLSASRLKGFRDVREKGMDKLIEKIKEEADANDGTVRVLKNARFAF 609
GPVWRSLRRNMVQNMLS SRLK FR+ RE MDKLIE+I+ +A N+ V LKNARFA
Sbjct: 120 GPVWRSLRRNMVQNMLSPSRLKEFREFREIAMDKLIERIRVDAKENNDVVWALKNARFAV 179
Query: 610 FCVLLTMCFGIELE-EEMIEKVDDMMKTVLITVDPRIDDFLPLLSPFF-YRQRKKALQVR 783
F +L+ MCFG+E++ EEMIE+VD MMK VLI +DPRIDDFLP+L F Y+QRK+ +VR
Sbjct: 180 FYILVAMCFGVEMDNEEMIERVDQMMKDVLIVLDPRIDDFLPILRLFVGYKQRKRVNEVR 239
Query: 784 QQQIDTIVPLIKRRRLALENPGSDKTATSFSYLDTLFELEVEGQKVNPSNAQLVTLCSEF 963
++QI+T+VPLI++RR ++NPGSDKTA SFSYLDTLF+++VEG+K P+NA+LVTLCSEF
Sbjct: 240 KRQIETLVPLIEKRRSVVQNPGSDKTAASFSYLDTLFDVKVEGRKSGPTNAELVTLCSEF 299
Query: 964 LNGGTDTTGTGIEWAMARIIENPHIQSRLYDEIKSVAGDKKVDEHDMQKMPYLNAFCKEL 1143
LNGGTDTT T +EW + R++ENP IQ++LY EIK++ GDKKVDE+D++KMPYLNA KEL
Sbjct: 300 LNGGTDTTATALEWGIGRLMENPTIQNQLYQEIKTIVGDKKVDENDIEKMPYLNAVVKEL 359
Query: 1144 LRRHPPTYFTLTHAVVEPTTLGGYD 1218
LR+HPPTYFTLTH+V EP L GYD
Sbjct: 360 LRKHPPTYFTLTHSVTEPVKLAGYD 384
Score = 174 bits (441), Expect(2) = 0.0
Identities = 77/108 (71%), Positives = 97/108 (89%)
Frame = +2
Query: 1301 EFFLQGISNDPKLWPNPDKFDPDRFLTGKEDPDITGVKDVRLVPFGIGRRICPGLGMASV 1480
EFF+ GIS+DP +W +P+KFDPDRFL+G+ED DITGVK+V+++PFG+GRRICPGLGMA+V
Sbjct: 392 EFFVHGISHDPNVWSDPEKFDPDRFLSGREDADITGVKEVKMMPFGVGRRICPGLGMATV 451
Query: 1481 HVNLMIARMVQAFEWSAYPENSKIDFTEGLEFTVIMKNSLIAKIKPRM 1624
HVNLM+ARMVQ FEW AYP N+K+DF+E LEFTV+MKN L AK+K R+
Sbjct: 452 HVNLMLARMVQEFEWFAYPGNNKVDFSEKLEFTVVMKNPLRAKVKLRI 499
>sp|P37124.1|C77A2_SOLME RecName: Full=Cytochrome P450 77A2; AltName: Full=CYPLXXVIIA2;
AltName: Full=Cytochrome P-450EG5
gi|438241|emb|CAA50646.1| CYP77A2 [Solanum melongena]
Length = 511
Score = 495 bits (1274), Expect(2) = e-180
Identities = 243/396 (61%), Positives = 306/396 (77%), Gaps = 1/396 (0%)
Frame = +1
Query: 34 FFIVLTTMEYHILVFVSIALFISSL-FFLTRXXXXXXXXXXXXXXXXXXIGNLFQVVGSG 210
FF + Y+ L+F +A ISS+ +FL++ +GNL QV SG
Sbjct: 3 FFSTSSLSSYYHLIFTILAFVISSIIYFLSKKAESKKLKLPPGPPGWPVVGNLLQVARSG 62
Query: 211 MPFFMYIRDVLIPKYGPILTIHMGTRTTIIVSDAELLREALIEKGQIFASRPMENPTRIV 390
PFF +R+ L KYGPI T+ MGTRT II+S+A+L+ EALI KGQ+FA+RP ENPTR V
Sbjct: 63 KPFFQIMRE-LRQKYGPIFTLRMGTRTMIILSNADLVHEALILKGQVFATRPRENPTRTV 121
Query: 391 FSCNKFAVNSSLYGPVWRSLRRNMVQNMLSASRLKGFRDVREKGMDKLIEKIKEEADAND 570
FSC+KF VN+++YGPVWRSLR+NMVQN LS+ RLK FR VR+ MDK+IEKI+ EADAN+
Sbjct: 122 FSCDKFTVNAAVYGPVWRSLRKNMVQNGLSSIRLKEFRAVRKSAMDKMIEKIRAEADANE 181
Query: 571 GTVRVLKNARFAFFCVLLTMCFGIELEEEMIEKVDDMMKTVLITVDPRIDDFLPLLSPFF 750
G V VLKNARFA FC+LL MCFG+E++E+ IEK+D MMK+VLI +DPR+DD+LP+LSPFF
Sbjct: 182 GVVWVLKNARFAVFCILLAMCFGVEMDEKTIEKIDQMMKSVLIALDPRLDDYLPILSPFF 241
Query: 751 YRQRKKALQVRQQQIDTIVPLIKRRRLALENPGSDKTATSFSYLDTLFELEVEGQKVNPS 930
+QRK A+ VR+QQI TIVP I++R+ LE+P DKTA SFSYLDTLF+L++EG+ P+
Sbjct: 242 SKQRKHAMDVRKQQIKTIVPFIEQRKKILESPEIDKTAASFSYLDTLFDLKIEGRNSTPT 301
Query: 931 NAQLVTLCSEFLNGGTDTTGTGIEWAMARIIENPHIQSRLYDEIKSVAGDKKVDEHDMQK 1110
+LVTLCSEFLNGGTDTT T IEWA+ R+IENP+IQS+LY+EIK G+ K+DE D++K
Sbjct: 302 YPELVTLCSEFLNGGTDTTATAIEWAIGRLIENPNIQSQLYEEIKKTVGENKIDEKDIEK 361
Query: 1111 MPYLNAFCKELLRRHPPTYFTLTHAVVEPTTLGGYD 1218
MPYLNA KELLR+HPPTY +LTHAV EP LGGYD
Sbjct: 362 MPYLNAVVKELLRKHPPTYMSLTHAVTEPAKLGGYD 397
Score = 165 bits (418), Expect(2) = e-180
Identities = 77/108 (71%), Positives = 92/108 (85%)
Frame = +2
Query: 1301 EFFLQGISNDPKLWPNPDKFDPDRFLTGKEDPDITGVKDVRLVPFGIGRRICPGLGMASV 1480
E FL GIS+DP LW P+KFDPDRF GKED DITGV V+++PFG+GRRICPGL MA+V
Sbjct: 405 EIFLPGISDDPNLWSEPEKFDPDRFYLGKEDADITGVSGVKMIPFGMGRRICPGLNMATV 464
Query: 1481 HVNLMIARMVQAFEWSAYPENSKIDFTEGLEFTVIMKNSLIAKIKPRM 1624
HV+LM+AR+VQ FEW A PEN+++DFTE LEFTV+MKN+L AKIKPRM
Sbjct: 465 HVSLMLARLVQEFEW-ADPENTRVDFTEKLEFTVVMKNTLRAKIKPRM 511