BLASTX nr result
ID: Bupleurum21_contig00007852
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00007852
(2273 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002278367.1| PREDICTED: KH domain-containing protein At4g... 384 e-104
emb|CAN73780.1| hypothetical protein VITISV_042181 [Vitis vinifera] 384 e-104
ref|XP_003531160.1| PREDICTED: KH domain-containing protein At4g... 371 e-100
ref|XP_002305041.1| predicted protein [Populus trichocarpa] gi|2... 355 4e-95
ref|XP_004169687.1| PREDICTED: KH domain-containing protein At4g... 344 5e-92
>ref|XP_002278367.1| PREDICTED: KH domain-containing protein At4g18375 [Vitis vinifera]
Length = 623
Score = 384 bits (987), Expect = e-104
Identities = 199/328 (60%), Positives = 236/328 (71%)
Frame = +3
Query: 3 SGKVINTIRQDSRAKVKVVDPFPGAKDRVLTIYCYXXXXXXXXXXXXXXXXXPLCAAQEA 182
SGKVIN+IRQD+RAK+KVVDPFPGAKDRV+TIYCY PLC AQ+A
Sbjct: 55 SGKVINSIRQDTRAKIKVVDPFPGAKDRVITIYCYVKEKEEVEVDDEFNDKQPLCPAQDA 114
Query: 183 LLMVHTVISNAMVTAGDSDKRQKDKEECQILVPSSQSANIIGKSGSTIKKLRSKTRTNIR 362
LL VH I NA+ + +SDK++KDKEECQILVPSSQSAN+IGK+G+TIK+LRSKTR NI+
Sbjct: 115 LLKVHAAIVNAVASLENSDKKKKDKEECQILVPSSQSANVIGKAGATIKRLRSKTRANIK 174
Query: 363 VTPKDASDPTHSCAMHFDNFIQITGDSEAVKRALFAISAIMYKFGPKEDISLDTXXXXXX 542
+T KD++DP+HSCAM FDNF+ I GDSEAVK+ALFAISAIMYKF P+E+I LDT
Sbjct: 175 ITAKDSTDPSHSCAMEFDNFVLIAGDSEAVKKALFAISAIMYKFTPREEIPLDTTVPEAP 234
Query: 543 XXXXXXXXXXMYPAAGLYPSLDSIVNSRSLSSIMSATHVPELSSYADKRSVWPVYSSSLP 722
+YPA G YPS DSIV++RS+ +++ATHVPEL Y+D S WPVYSS+LP
Sbjct: 235 PSIIIPSDVPIYPAGGFYPSADSIVSARSVPPVLAATHVPELQGYSDMGSTWPVYSSNLP 294
Query: 723 VVSGYGGASRSEELIIKVLCSSSKXXXXXXXXXXXXXXXXXXXXXXXXVDDKKIDGNECA 902
VVSGY GASRSEELII+VLC K VDD K D +EC
Sbjct: 295 VVSGY-GASRSEELIIRVLCPFDKIGRVIGRGGSSIKSVREASGAHVEVDDTKADRDECL 353
Query: 903 ITITSTEKLDDVKSMAVEAVLLLQEKIN 986
IT+TSTE +DD+KSMAVEAVLLLQ KIN
Sbjct: 354 ITVTSTESVDDLKSMAVEAVLLLQAKIN 381
Score = 165 bits (418), Expect = 4e-38
Identities = 99/215 (46%), Positives = 122/215 (56%), Gaps = 2/215 (0%)
Frame = +2
Query: 1154 NEIRKRTKADVHISKGDKPKRAD--DELXXXXXXXXXXRDALIQIXXXXXXXXXXXXEGN 1327
NEIRKRT+ADV ISK ++PK AD DEL RDAL+QI +
Sbjct: 411 NEIRKRTRADVRISKSERPKCADANDELIEVVGEVGSVRDALVQIVLRLRDDALKDRDTG 470
Query: 1328 RIXXXXXXXXXXXXXXXXXXMPTVLSSISQAGHPGYEKRGDGGSGINMLSSDSFYGYGSL 1507
+ +VL S Y++R + GSG+ +LSS S YGYGSL
Sbjct: 471 H--NTSAGTDSLYSGGAGLSVSSVLPSAPPVAPLSYDQRAETGSGLGLLSSSSLYGYGSL 528
Query: 1508 PTXXXXXXXXXXXXXXXXXXXXPPTDLEMVVPAHAVGKVMGKGGANLENIRKISGAVVDV 1687
P+ +MV+PA+AVGKVMGKGGAN+ NIRKISGAV+++
Sbjct: 529 SVGENAYGSMSSYSSKLYGGLPTPSTFDMVIPANAVGKVMGKGGANIANIRKISGAVIEI 588
Query: 1688 SHSKSSRGDRVAVISGTSEQKRAAENLIKAFIMST 1792
S SKSSRGDRVA+ISGT EQKR AENLI+AFIM+T
Sbjct: 589 SDSKSSRGDRVALISGTPEQKRTAENLIQAFIMAT 623
>emb|CAN73780.1| hypothetical protein VITISV_042181 [Vitis vinifera]
Length = 637
Score = 384 bits (987), Expect = e-104
Identities = 199/328 (60%), Positives = 236/328 (71%)
Frame = +3
Query: 3 SGKVINTIRQDSRAKVKVVDPFPGAKDRVLTIYCYXXXXXXXXXXXXXXXXXPLCAAQEA 182
SGKVIN+IRQD+RAK+KVVDPFPGAKDRV+TIYCY PLC AQ+A
Sbjct: 55 SGKVINSIRQDTRAKIKVVDPFPGAKDRVITIYCYVKEKEEVEVDDEFNDKQPLCPAQDA 114
Query: 183 LLMVHTVISNAMVTAGDSDKRQKDKEECQILVPSSQSANIIGKSGSTIKKLRSKTRTNIR 362
LL VH I NA+ + +SDK++KDKEECQILVPSSQSAN+IGK+G+TIK+LRSKTR NI+
Sbjct: 115 LLKVHAAIVNAVASLENSDKKKKDKEECQILVPSSQSANVIGKAGATIKRLRSKTRANIK 174
Query: 363 VTPKDASDPTHSCAMHFDNFIQITGDSEAVKRALFAISAIMYKFGPKEDISLDTXXXXXX 542
+T KD++DP+HSCAM FDNF+ I GDSEAVK+ALFAISAIMYKF P+E+I LDT
Sbjct: 175 ITAKDSTDPSHSCAMEFDNFVLIAGDSEAVKKALFAISAIMYKFTPREEIPLDTTVPEAP 234
Query: 543 XXXXXXXXXXMYPAAGLYPSLDSIVNSRSLSSIMSATHVPELSSYADKRSVWPVYSSSLP 722
+YPA G YPS DSIV++RS+ +++ATHVPEL Y+D S WPVYSS+LP
Sbjct: 235 PSIIIPSDVPIYPAGGFYPSADSIVSARSVPPVLAATHVPELQGYSDMGSTWPVYSSNLP 294
Query: 723 VVSGYGGASRSEELIIKVLCSSSKXXXXXXXXXXXXXXXXXXXXXXXXVDDKKIDGNECA 902
VVSGY GASRSEELII+VLC K VDD K D +EC
Sbjct: 295 VVSGY-GASRSEELIIRVLCPFDKIGRVIGRGGSSIKSVREASGAHVEVDDTKADRDECL 353
Query: 903 ITITSTEKLDDVKSMAVEAVLLLQEKIN 986
IT+TSTE +DD+KSMAVEAVLLLQ KIN
Sbjct: 354 ITVTSTESVDDLKSMAVEAVLLLQAKIN 381
Score = 155 bits (393), Expect = 3e-35
Identities = 99/229 (43%), Positives = 122/229 (53%), Gaps = 16/229 (6%)
Frame = +2
Query: 1154 NEIRKRTKADVHISKGDKPKRAD--DELXXXXXXXXXXRDALIQIXXXXXXXXXXXXEGN 1327
NEIRKRT+ADV ISK ++PK AD DEL RDAL+QI +
Sbjct: 411 NEIRKRTRADVRISKSERPKCADANDELIEVVGEVGSVRDALVQIVLRLRDDALKDRDTG 470
Query: 1328 RIXXXXXXXXXXXXXXXXXXMPTVLSSISQAGHPGYEKRGDGGSGINMLSSDSFYGYGSL 1507
+ +VL S Y++R + GSG+ +LSS S YGYGSL
Sbjct: 471 H--NTSAGTDSLYSGGAGLSVSSVLPSAPPVAPLSYDQRAETGSGLGLLSSSSLYGYGSL 528
Query: 1508 P--------------TXXXXXXXXXXXXXXXXXXXXPPTDLEMVVPAHAVGKVMGKGGAN 1645
P+ +MV+PA+AVGKVMGKGGAN
Sbjct: 529 SLFFAVKVVVVFNGEVGENAYGSMSSYSSKLYGGLPTPSTFDMVIPANAVGKVMGKGGAN 588
Query: 1646 LENIRKISGAVVDVSHSKSSRGDRVAVISGTSEQKRAAENLIKAFIMST 1792
+ NIRKISGAV+++S SKSSRGDRVA+ISGT EQKR AENLI+AFIM+T
Sbjct: 589 IANIRKISGAVIEISDSKSSRGDRVALISGTPEQKRTAENLIQAFIMAT 637
>ref|XP_003531160.1| PREDICTED: KH domain-containing protein At4g18375-like [Glycine max]
Length = 625
Score = 371 bits (952), Expect = e-100
Identities = 193/328 (58%), Positives = 234/328 (71%)
Frame = +3
Query: 3 SGKVINTIRQDSRAKVKVVDPFPGAKDRVLTIYCYXXXXXXXXXXXXXXXXXPLCAAQEA 182
+GKVIN+IRQ++RAKVKVVDPFPG+KDRV+TIYCY PLCAAQ+A
Sbjct: 55 NGKVINSIRQETRAKVKVVDPFPGSKDRVITIYCYVKEKEDVEIDDEFAGKEPLCAAQDA 114
Query: 183 LLMVHTVISNAMVTAGDSDKRQKDKEECQILVPSSQSANIIGKSGSTIKKLRSKTRTNIR 362
LL VH I+N++ GDS+K++KD++ECQILVPSSQSANIIGK+G+TIKKLRSKTR NI+
Sbjct: 115 LLKVHVAIANSIAAIGDSEKKRKDRDECQILVPSSQSANIIGKAGATIKKLRSKTRANIK 174
Query: 363 VTPKDASDPTHSCAMHFDNFIQITGDSEAVKRALFAISAIMYKFGPKEDISLDTXXXXXX 542
VT KDA+DPTHSCAM FDNF+ ITG+SEAVKRALFA+S+IMYKFGP+EDISLDT
Sbjct: 175 VTAKDAADPTHSCAMEFDNFVVITGESEAVKRALFAVSSIMYKFGPREDISLDTAVPEAP 234
Query: 543 XXXXXXXXXXMYPAAGLYPSLDSIVNSRSLSSIMSATHVPELSSYADKRSVWPVYSSSLP 722
+YP GLYP+ D IV R++ I+ AT+VP+L YAD + WP+YSS+LP
Sbjct: 235 PSIIIPSDVPVYPPGGLYPASDPIVTPRAVPQIIGATNVPDLQGYADAGNSWPMYSSALP 294
Query: 723 VVSGYGGASRSEELIIKVLCSSSKXXXXXXXXXXXXXXXXXXXXXXXXVDDKKIDGNECA 902
VVSG GASRSEELII++LC S K VDD K + +EC
Sbjct: 295 VVSGV-GASRSEELIIRMLCPSDKIGRVIGKGGSTIKSMRQASGAHIEVDDSKANFDECL 353
Query: 903 ITITSTEKLDDVKSMAVEAVLLLQEKIN 986
I IT+TE D+KSMAVEAVLL+Q KIN
Sbjct: 354 IIITTTESPSDLKSMAVEAVLLMQGKIN 381
Score = 166 bits (421), Expect = 2e-38
Identities = 101/217 (46%), Positives = 127/217 (58%), Gaps = 4/217 (1%)
Frame = +2
Query: 1154 NEIRKRTKADVHISKGDKPKRAD--DELXXXXXXXXXXRDALIQIXXXXXXXXXXXXEGN 1327
NEIRKRTKADV ISKGDKPK AD DEL RDALIQI +
Sbjct: 411 NEIRKRTKADVRISKGDKPKCADANDELVEVGGSVDCVRDALIQIILRLRDDVLRERDTG 470
Query: 1328 RIXXXXXXXXXXXXXXXXXXMPTVLSSISQAGHPG-YEKRGDGGSGINMLSSDSFYG-YG 1501
+P+++ S+ P Y+ R + G+G+ MLS S YG YG
Sbjct: 471 H--NPSIGAESLYPGSAGLSLPSMMHSVPPVAAPMVYDHRAESGAGLGMLSPSSPYGGYG 528
Query: 1502 SLPTXXXXXXXXXXXXXXXXXXXXPPTDLEMVVPAHAVGKVMGKGGANLENIRKISGAVV 1681
SLP PP+ L+M++PA+AVGKV+GKGGAN+ NIRKISGA +
Sbjct: 529 SLPMGDNGYGSMSSYATKLYGGLPPPSTLDMLIPANAVGKVLGKGGANIANIRKISGASI 588
Query: 1682 DVSHSKSSRGDRVAVISGTSEQKRAAENLIKAFIMST 1792
++S +KS+RGDR+A+ISGT EQKRAAENLI+AFIM+T
Sbjct: 589 EISDNKSARGDRIALISGTPEQKRAAENLIQAFIMAT 625
>ref|XP_002305041.1| predicted protein [Populus trichocarpa] gi|222848005|gb|EEE85552.1|
predicted protein [Populus trichocarpa]
Length = 623
Score = 355 bits (910), Expect = 4e-95
Identities = 186/328 (56%), Positives = 222/328 (67%)
Frame = +3
Query: 3 SGKVINTIRQDSRAKVKVVDPFPGAKDRVLTIYCYXXXXXXXXXXXXXXXXXPLCAAQEA 182
SGKVIN+IR +SRA+VKVVDPFPGA RV+TIYC PLC AQ+A
Sbjct: 54 SGKVINSIRNESRARVKVVDPFPGAMYRVITIYCNVKEKGDADVDDDFHQADPLCPAQDA 113
Query: 183 LLMVHTVISNAMVTAGDSDKRQKDKEECQILVPSSQSANIIGKSGSTIKKLRSKTRTNIR 362
LL VH ISNA+ GDSDKR +DK+ECQILVP+SQSANIIGK+G+TIKKLRSKTR NI+
Sbjct: 114 LLKVHAAISNAVAALGDSDKRCRDKKECQILVPTSQSANIIGKAGATIKKLRSKTRANIK 173
Query: 363 VTPKDASDPTHSCAMHFDNFIQITGDSEAVKRALFAISAIMYKFGPKEDISLDTXXXXXX 542
+T KDASDPTHSCAM FDNF+ ITG+SEAVK+ALFA+SAIMYKF PKE+I L+T
Sbjct: 174 ITAKDASDPTHSCAMDFDNFLLITGESEAVKKALFAVSAIMYKFSPKEEIPLETTVPEAP 233
Query: 543 XXXXXXXXXXMYPAAGLYPSLDSIVNSRSLSSIMSATHVPELSSYADKRSVWPVYSSSLP 722
+Y G YP+ D IV+SRS+ I+ ATH+PE Y D RS WP+YSS++P
Sbjct: 234 PSIIISSDVPIYQPGGFYPNADPIVSSRSVPPILGATHIPEFQGYGDMRSSWPIYSSTVP 293
Query: 723 VVSGYGGASRSEELIIKVLCSSSKXXXXXXXXXXXXXXXXXXXXXXXXVDDKKIDGNECA 902
VV +G SRS ELII++LC K VDD K D +EC
Sbjct: 294 VVPSFGNTSRS-ELIIRLLCPFDKIGRVIGKGGSTIKSIRQVSGARIEVDDTKADRDECI 352
Query: 903 ITITSTEKLDDVKSMAVEAVLLLQEKIN 986
IT+ +TE DD+KSMAVEA+LLLQ KIN
Sbjct: 353 ITVIATESPDDLKSMAVEAILLLQGKIN 380
Score = 164 bits (416), Expect = 7e-38
Identities = 99/216 (45%), Positives = 125/216 (57%), Gaps = 3/216 (1%)
Frame = +2
Query: 1154 NEIRKRTKADVHISKGDKPKRAD--DELXXXXXXXXXXRDALIQIXXXXXXXXXXXXEGN 1327
NEIRKRT ADV ISK DK K AD DEL RDAL+QI +G
Sbjct: 410 NEIRKRTNADVCISKVDKLKCADSNDELVEVAGEVGSVRDALVQIVLRLRDDVLKEKDGG 469
Query: 1328 RIXXXXXXXXXXXXXXXXXXMPTVLSSISQAGHPGYEKRGDGGSGINMLSSDSFYGYGSL 1507
+P++L S+ GY++R + GSG+ + SS S YGYGSL
Sbjct: 470 --LNSSVGTDSVYPVHAGISIPSILPSVPPVAPMGYDQRAESGSGLGLFSSSSLYGYGSL 527
Query: 1508 PTXXXXXXXXXXXXXXXXXXXXPP-TDLEMVVPAHAVGKVMGKGGANLENIRKISGAVVD 1684
P PP + LEM+VPA+AVGKV+GKGGAN+ NIRKISGA+++
Sbjct: 528 PMGENSYGSLASYSLSKLYEGLPPPSTLEMLVPANAVGKVIGKGGANIANIRKISGAMIE 587
Query: 1685 VSHSKSSRGDRVAVISGTSEQKRAAENLIKAFIMST 1792
+S +KS+RGDR+A ISG EQK+AAENLI+AFIM+T
Sbjct: 588 ISDAKSARGDRIAYISGKPEQKQAAENLIQAFIMAT 623
>ref|XP_004169687.1| PREDICTED: KH domain-containing protein At4g18375-like, partial
[Cucumis sativus]
Length = 531
Score = 344 bits (883), Expect = 5e-92
Identities = 179/329 (54%), Positives = 222/329 (67%), Gaps = 1/329 (0%)
Frame = +3
Query: 3 SGKVINTIRQDSRAKVKVVDPFPGAKDRVLTIYCYXXXXXXXXXXXXXXXXXPLCAAQEA 182
SGKVIN+IRQ++RAK+KVVDPFPGAKDRV+TI+C+ LCAAQ+A
Sbjct: 54 SGKVINSIRQETRAKIKVVDPFPGAKDRVITIFCFVKDKEDVEVDDEFNDRQTLCAAQDA 113
Query: 183 LLMVHTVISNAMVTAGDSDKRQKDKEECQILVPSSQSANIIGKSGSTIKKLRSKTRTNIR 362
LL VH I+NA+ +AGD D++Q+DKE+CQIL+PSSQSAN+IGK+GSTIKKLRSKTRT+I+
Sbjct: 114 LLKVHAAIANALASAGDLDRKQRDKEQCQILIPSSQSANVIGKAGSTIKKLRSKTRTSIK 173
Query: 363 VTPKDASDPTHSCAMHFDNFIQITGDSEAVKRALFAISAIMYKFGPKEDISLDTXXXXXX 542
++PKDA+DP HSCAM FDNF I G+ E V+RALFAIS+IMYKF P+E+I LDT
Sbjct: 174 ISPKDATDPIHSCAMDFDNFAVIAGEPEGVRRALFAISSIMYKFPPREEIPLDTNVNEAP 233
Query: 543 XXXXXXXXXXMYPAAGLYPSLDSIVNSRSLSSIMSATHVPELSSYADKRSVWPVYSSSLP 722
+Y A GLYPS D I+ RS+ ++ A HV +L Y + + WP+YSSSLP
Sbjct: 234 PNIIIPSDVPLYSAGGLYPSADPILPPRSIPPMLGAQHVQDLHDYPESGNAWPLYSSSLP 293
Query: 723 VV-SGYGGASRSEELIIKVLCSSSKXXXXXXXXXXXXXXXXXXXXXXXXVDDKKIDGNEC 899
VV G G SEEL+++VLC VDD K D +EC
Sbjct: 294 VVPGGISGHPPSEELVVRVLCPFDNIGRVIGKGGGTIKSIRQASGARVEVDDTKRDCDEC 353
Query: 900 AITITSTEKLDDVKSMAVEAVLLLQEKIN 986
ITI+S E LDD+KSMAVE VLLLQEKIN
Sbjct: 354 IITISSFESLDDLKSMAVETVLLLQEKIN 382
Score = 65.9 bits (159), Expect = 5e-08
Identities = 46/121 (38%), Positives = 57/121 (47%), Gaps = 3/121 (2%)
Frame = +2
Query: 1154 NEIRKRTKADVHISKGDKPKRAD--DELXXXXXXXXXXRDALIQIXXXXXXXXXXXXEGN 1327
NEIRK T+AD+ ISKGDK K A DEL RDAL+QI +
Sbjct: 412 NEIRKSTRADIRISKGDKLKCASSSDELVEVTGKVGCVRDALVQIVLRLRDDALKERDVG 471
Query: 1328 RIXXXXXXXXXXXXXXXXXXMPTVLSSISQAGHP-GYEKRGDGGSGINMLSSDSFYGYGS 1504
MP+VL S+S P GY++R + GSG+ +LSS YG GS
Sbjct: 472 H--NSAIVTDSMYSSGSSFSMPSVLPSVSPGAPPMGYDQRAESGSGLGVLSSSGLYGSGS 529
Query: 1505 L 1507
L
Sbjct: 530 L 530