BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00007751
         (1534 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002284901.1| PREDICTED: uncharacterized protein LOC100258...   442   e-175
emb|CBI16268.3| unnamed protein product [Vitis vinifera]              442   e-175
gb|AFK76482.1| tRNA ligase [Solanum melongena]                        423   e-168
ref|XP_002323444.1| predicted protein [Populus trichocarpa] gi|2...   423   e-163
ref|XP_004147268.1| PREDICTED: uncharacterized protein LOC101214...   427   e-162

>ref|XP_002284901.1| PREDICTED: uncharacterized protein LOC100258617 [Vitis vinifera]
          Length = 1165

 Score =  442 bits (1138), Expect(2) = e-175
 Identities = 211/260 (81%), Positives = 238/260 (91%)
 Frame = -1

Query: 1534 IPGCAKSALCKEILSSTGGLGDDRPLHSLMGDLVKGKYWQKVAEHRKKKPYSIMLADKNA 1355
            IPGCAKSALCKEILS+ GG GDDRP+HSLMGDL+KG+YW KVAE R++KP SI+LADKNA
Sbjct: 758  IPGCAKSALCKEILSAPGGFGDDRPVHSLMGDLIKGRYWPKVAEERRRKPCSIILADKNA 817

Query: 1354 PNEDVWRQIESMCRSTKASAVPVVPDSEGTETNPFSLDALAVFTFRVLQRVNHPGNLDKA 1175
            PNE+VWRQIE MCRST+ASAVPVVPDSEGT++NPFSLDALAVF FRVLQRVNHPGNLDKA
Sbjct: 818  PNEEVWRQIEDMCRSTRASAVPVVPDSEGTDSNPFSLDALAVFMFRVLQRVNHPGNLDKA 877

Query: 1174 SPNACYVLLVFYHLYEGKNRREFESELIERFGSLVKMPLLKPDRIPLPESIKSTLEEGIN 995
            SPNA YVLL+FYHLYEGK+R+EFESELIERFGSLVKMPLLK DR  +P+S+K+ LEEGIN
Sbjct: 878  SPNAGYVLLMFYHLYEGKSRKEFESELIERFGSLVKMPLLKSDRSTMPDSVKNCLEEGIN 937

Query: 994  LYKLHSNRHGRSDSTKGTYAQEWAKWEKQLRDILSENVEYLNSVQVPFESTVKQVLEQLK 815
            LY+LH+NRHGR +STKGTYA EW+KWEKQLRDIL +N EYL S+QVPFES+V+QVLEQLK
Sbjct: 938  LYRLHTNRHGRLESTKGTYANEWSKWEKQLRDILFDNAEYLTSIQVPFESSVRQVLEQLK 997

Query: 814  IIAKGDYRTPSTVKRKLGSI 755
             IAKGDY TP T KRK G+I
Sbjct: 998  SIAKGDYPTPGTEKRKFGTI 1017



 Score =  202 bits (513), Expect(2) = e-175
 Identities = 98/148 (66%), Positives = 121/148 (81%), Gaps = 2/148 (1%)
 Frame = -2

Query: 759  VYAAVNLPITEIQKLIDNLGEKDSKLKAFLKDKNM--ATKNAHLTLAHKRSHGVTAVANF 586
            V+AAV+LP+TEIQ L+ NL EK+ K++AF KDK++  + +NAH+TLAHKRSHGVTAVAN+
Sbjct: 1018 VFAAVSLPVTEIQSLLANLAEKNPKVEAFFKDKHLENSLRNAHVTLAHKRSHGVTAVANY 1077

Query: 585  GSVLHYNVPVDMTGLLFSDKLAALEAHPGSCDGEKVTSKNVWPHVTLWTAEGIAAKEANN 406
            G  L+  VPVD T LLFSDK+AALEA+PGS DGE++TSKN WPHVTLWT  G+A KEAN 
Sbjct: 1078 GLFLNRQVPVDFTALLFSDKMAALEAYPGSVDGERITSKNQWPHVTLWTGAGVAPKEANM 1137

Query: 405  LPQLFSEGKAARIVIDPPVTGIGTVEFY 322
            LP+L SEG A RI I PP+T  GT+EF+
Sbjct: 1138 LPELISEGTATRIDISPPITISGTLEFF 1165


>emb|CBI16268.3| unnamed protein product [Vitis vinifera]
          Length = 1029

 Score =  442 bits (1138), Expect(2) = e-175
 Identities = 211/260 (81%), Positives = 238/260 (91%)
 Frame = -1

Query: 1534 IPGCAKSALCKEILSSTGGLGDDRPLHSLMGDLVKGKYWQKVAEHRKKKPYSIMLADKNA 1355
            IPGCAKSALCKEILS+ GG GDDRP+HSLMGDL+KG+YW KVAE R++KP SI+LADKNA
Sbjct: 622  IPGCAKSALCKEILSAPGGFGDDRPVHSLMGDLIKGRYWPKVAEERRRKPCSIILADKNA 681

Query: 1354 PNEDVWRQIESMCRSTKASAVPVVPDSEGTETNPFSLDALAVFTFRVLQRVNHPGNLDKA 1175
            PNE+VWRQIE MCRST+ASAVPVVPDSEGT++NPFSLDALAVF FRVLQRVNHPGNLDKA
Sbjct: 682  PNEEVWRQIEDMCRSTRASAVPVVPDSEGTDSNPFSLDALAVFMFRVLQRVNHPGNLDKA 741

Query: 1174 SPNACYVLLVFYHLYEGKNRREFESELIERFGSLVKMPLLKPDRIPLPESIKSTLEEGIN 995
            SPNA YVLL+FYHLYEGK+R+EFESELIERFGSLVKMPLLK DR  +P+S+K+ LEEGIN
Sbjct: 742  SPNAGYVLLMFYHLYEGKSRKEFESELIERFGSLVKMPLLKSDRSTMPDSVKNCLEEGIN 801

Query: 994  LYKLHSNRHGRSDSTKGTYAQEWAKWEKQLRDILSENVEYLNSVQVPFESTVKQVLEQLK 815
            LY+LH+NRHGR +STKGTYA EW+KWEKQLRDIL +N EYL S+QVPFES+V+QVLEQLK
Sbjct: 802  LYRLHTNRHGRLESTKGTYANEWSKWEKQLRDILFDNAEYLTSIQVPFESSVRQVLEQLK 861

Query: 814  IIAKGDYRTPSTVKRKLGSI 755
             IAKGDY TP T KRK G+I
Sbjct: 862  SIAKGDYPTPGTEKRKFGTI 881



 Score =  202 bits (513), Expect(2) = e-175
 Identities = 98/148 (66%), Positives = 121/148 (81%), Gaps = 2/148 (1%)
 Frame = -2

Query: 759  VYAAVNLPITEIQKLIDNLGEKDSKLKAFLKDKNM--ATKNAHLTLAHKRSHGVTAVANF 586
            V+AAV+LP+TEIQ L+ NL EK+ K++AF KDK++  + +NAH+TLAHKRSHGVTAVAN+
Sbjct: 882  VFAAVSLPVTEIQSLLANLAEKNPKVEAFFKDKHLENSLRNAHVTLAHKRSHGVTAVANY 941

Query: 585  GSVLHYNVPVDMTGLLFSDKLAALEAHPGSCDGEKVTSKNVWPHVTLWTAEGIAAKEANN 406
            G  L+  VPVD T LLFSDK+AALEA+PGS DGE++TSKN WPHVTLWT  G+A KEAN 
Sbjct: 942  GLFLNRQVPVDFTALLFSDKMAALEAYPGSVDGERITSKNQWPHVTLWTGAGVAPKEANM 1001

Query: 405  LPQLFSEGKAARIVIDPPVTGIGTVEFY 322
            LP+L SEG A RI I PP+T  GT+EF+
Sbjct: 1002 LPELISEGTATRIDISPPITISGTLEFF 1029


>gb|AFK76482.1| tRNA ligase [Solanum melongena]
          Length = 1167

 Score =  423 bits (1088), Expect(2) = e-168
 Identities = 196/260 (75%), Positives = 238/260 (91%)
 Frame = -1

Query: 1534 IPGCAKSALCKEILSSTGGLGDDRPLHSLMGDLVKGKYWQKVAEHRKKKPYSIMLADKNA 1355
            IPGCAKSALCKEIL++ GGLGDDRP++SLMGDL+KG+YWQKVA+ R++KPYSIMLADKNA
Sbjct: 761  IPGCAKSALCKEILNAPGGLGDDRPVNSLMGDLIKGRYWQKVADERRRKPYSIMLADKNA 820

Query: 1354 PNEDVWRQIESMCRSTKASAVPVVPDSEGTETNPFSLDALAVFTFRVLQRVNHPGNLDKA 1175
            PNE+VW+QIE+MC ST ASA+PV+PDSEGTETNPFS+DALAVF FRVL RVNHPGNLDK+
Sbjct: 821  PNEEVWKQIENMCLSTGASAIPVIPDSEGTETNPFSIDALAVFIFRVLHRVNHPGNLDKS 880

Query: 1174 SPNACYVLLVFYHLYEGKNRREFESELIERFGSLVKMPLLKPDRIPLPESIKSTLEEGIN 995
            SPNA YV+L+FYHLY+GK+R+EFESELIERFGSLV++P+LKP+R PLP+S++S +EEG++
Sbjct: 881  SPNAGYVMLMFYHLYDGKSRQEFESELIERFGSLVRIPVLKPERSPLPDSVRSIIEEGLS 940

Query: 994  LYKLHSNRHGRSDSTKGTYAQEWAKWEKQLRDILSENVEYLNSVQVPFESTVKQVLEQLK 815
            LY+LH+ +HGR +STKGTY QEW KWEKQLRDIL  N +YLNS+QVPFE  VK+VLEQLK
Sbjct: 941  LYRLHTTKHGRLESTKGTYVQEWVKWEKQLRDILLGNADYLNSIQVPFEFAVKEVLEQLK 1000

Query: 814  IIAKGDYRTPSTVKRKLGSI 755
            +IA+G+Y  P+  KRKLGSI
Sbjct: 1001 VIARGEYAVPAE-KRKLGSI 1019



 Score =  197 bits (500), Expect(2) = e-168
 Identities = 95/148 (64%), Positives = 120/148 (81%), Gaps = 2/148 (1%)
 Frame = -2

Query: 759  VYAAVNLPITEIQKLIDNLGEKDSKLKAFLKDKNMAT--KNAHLTLAHKRSHGVTAVANF 586
            V+AA++LP+ EI  L+++L +KD K+  F+KDK+M +  + AHLTLAHKRSHGVTAVAN+
Sbjct: 1020 VFAAISLPVPEILGLLNDLAKKDPKVGDFIKDKSMESSIQKAHLTLAHKRSHGVTAVANY 1079

Query: 585  GSVLHYNVPVDMTGLLFSDKLAALEAHPGSCDGEKVTSKNVWPHVTLWTAEGIAAKEANN 406
            GS LH  VPVD+  LLFSDKLAALEA PGS +GEK+ SKN WPH+TLW+  G+AAK+AN 
Sbjct: 1080 GSFLHQKVPVDVAALLFSDKLAALEAEPGSVEGEKINSKNSWPHITLWSGAGVAAKDANT 1139

Query: 405  LPQLFSEGKAARIVIDPPVTGIGTVEFY 322
            LPQL S+GKA RI I+PPVT  GT+EF+
Sbjct: 1140 LPQLLSQGKATRIDINPPVTITGTLEFF 1167


>ref|XP_002323444.1| predicted protein [Populus trichocarpa] gi|222868074|gb|EEF05205.1|
            predicted protein [Populus trichocarpa]
          Length = 1152

 Score =  423 bits (1087), Expect(2) = e-163
 Identities = 197/260 (75%), Positives = 233/260 (89%)
 Frame = -1

Query: 1534 IPGCAKSALCKEILSSTGGLGDDRPLHSLMGDLVKGKYWQKVAEHRKKKPYSIMLADKNA 1355
            IPGCAKS LCKE+L++ GGLGDDRP+HSLMGDL+KGKYWQK+A+ R+KKPYS++LADKNA
Sbjct: 734  IPGCAKSVLCKELLNAPGGLGDDRPVHSLMGDLIKGKYWQKIADERRKKPYSVILADKNA 793

Query: 1354 PNEDVWRQIESMCRSTKASAVPVVPDSEGTETNPFSLDALAVFTFRVLQRVNHPGNLDKA 1175
            PNE+VWRQIE MCRST+ASAVPV+PDSEGT++NPFSLDALAVF FRVLQRVNHPGNLDK+
Sbjct: 794  PNEEVWRQIEGMCRSTQASAVPVIPDSEGTDSNPFSLDALAVFMFRVLQRVNHPGNLDKS 853

Query: 1174 SPNACYVLLVFYHLYEGKNRREFESELIERFGSLVKMPLLKPDRIPLPESIKSTLEEGIN 995
            SPNA +VLL+FYHLY+GKNR EFESELIERFGSLVKMPLL+PDR  LP+ ++  LEEGIN
Sbjct: 854  SPNAGFVLLMFYHLYDGKNRTEFESELIERFGSLVKMPLLRPDRSSLPDPVRLILEEGIN 913

Query: 994  LYKLHSNRHGRSDSTKGTYAQEWAKWEKQLRDILSENVEYLNSVQVPFESTVKQVLEQLK 815
            LY+LH+N HGR +STKG+Y +EW KWEKQLR++L  + E+LNS+QVPFES VKQV EQL+
Sbjct: 914  LYRLHTNAHGRLESTKGSYGKEWVKWEKQLREVLIGSAEHLNSIQVPFESAVKQVSEQLQ 973

Query: 814  IIAKGDYRTPSTVKRKLGSI 755
             I KG+Y  PST  RKLG+I
Sbjct: 974  NIIKGEYTPPSTEMRKLGTI 993



 Score =  179 bits (454), Expect(2) = e-163
 Identities = 94/159 (59%), Positives = 112/159 (70%), Gaps = 13/159 (8%)
 Frame = -2

Query: 759  VYAAVNLPITEIQKLIDNLG-----------EKDSKLKAFLKDKNMA--TKNAHLTLAHK 619
            ++AAV+LP TEI  L+D                + K+K+FLKDK+M    K AHLTLAHK
Sbjct: 994  IFAAVSLPATEISSLLDKASIMLCYYFLPSLSNNPKVKSFLKDKDMEHNLKKAHLTLAHK 1053

Query: 618  RSHGVTAVANFGSVLHYNVPVDMTGLLFSDKLAALEAHPGSCDGEKVTSKNVWPHVTLWT 439
            RSHGVTAVA +G +LH  VPV++T LLF+D++AALEA  GS DGEKV  KN WPHVTLWT
Sbjct: 1054 RSHGVTAVARYGHLLHQKVPVELTALLFTDEMAALEAEVGSVDGEKVIPKNEWPHVTLWT 1113

Query: 438  AEGIAAKEANNLPQLFSEGKAARIVIDPPVTGIGTVEFY 322
             E IAAKEAN LPQL  EGKA RI I+PP+   G +EFY
Sbjct: 1114 GEKIAAKEANRLPQLLLEGKAIRIEINPPIIISGELEFY 1152


>ref|XP_004147268.1| PREDICTED: uncharacterized protein LOC101214899 [Cucumis sativus]
          Length = 1135

 Score =  427 bits (1097), Expect(2) = e-162
 Identities = 200/260 (76%), Positives = 236/260 (90%)
 Frame = -1

Query: 1534 IPGCAKSALCKEILSSTGGLGDDRPLHSLMGDLVKGKYWQKVAEHRKKKPYSIMLADKNA 1355
            IPGCAKSALCKEIL + G LGDDRP+++LMGDL+KG+YWQKVA+ R++KPYSIMLADKNA
Sbjct: 728  IPGCAKSALCKEILKAPGALGDDRPVNTLMGDLIKGRYWQKVADDRRRKPYSIMLADKNA 787

Query: 1354 PNEDVWRQIESMCRSTKASAVPVVPDSEGTETNPFSLDALAVFTFRVLQRVNHPGNLDKA 1175
            PNE+VWRQIE MCRST+ASAVPV+PDSEGT++NPFSLDALAVF FRVLQRVNHPGNLDKA
Sbjct: 788  PNEEVWRQIEDMCRSTRASAVPVIPDSEGTDSNPFSLDALAVFMFRVLQRVNHPGNLDKA 847

Query: 1174 SPNACYVLLVFYHLYEGKNRREFESELIERFGSLVKMPLLKPDRIPLPESIKSTLEEGIN 995
            SPNA YVLL+FYHLY+GK+RREFE ELI+RFGSLVKMPLLK DR PLP+ +K+ LEEGI+
Sbjct: 848  SPNAGYVLLMFYHLYDGKSRREFEGELIDRFGSLVKMPLLKSDRNPLPDDLKTILEEGIS 907

Query: 994  LYKLHSNRHGRSDSTKGTYAQEWAKWEKQLRDILSENVEYLNSVQVPFESTVKQVLEQLK 815
            LYKLH++RHGR DSTKG+YA+EWAKWEKQLR+ L  N EYLN++QVPFE  V+ VLEQLK
Sbjct: 908  LYKLHTSRHGRVDSTKGSYAKEWAKWEKQLRETLFSNTEYLNAIQVPFELAVQDVLEQLK 967

Query: 814  IIAKGDYRTPSTVKRKLGSI 755
             ++KGDY++P T +RK G+I
Sbjct: 968  KVSKGDYKSPITERRKSGAI 987



 Score =  171 bits (434), Expect(2) = e-162
 Identities = 85/148 (57%), Positives = 112/148 (75%), Gaps = 2/148 (1%)
 Frame = -2

Query: 759  VYAAVNLPITEIQKLIDNLGEKDSKLKAFLKD--KNMATKNAHLTLAHKRSHGVTAVANF 586
            V+AAV+LP+ EIQ L+  L +K+S+++AFL++  K+   K AH+TLAHKRSHGV  VA++
Sbjct: 988  VFAAVSLPVQEIQNLLGTLAKKNSRIEAFLREHYKDYKLKGAHVTLAHKRSHGVKGVADY 1047

Query: 585  GSVLHYNVPVDMTGLLFSDKLAALEAHPGSCDGEKVTSKNVWPHVTLWTAEGIAAKEANN 406
            G   +  VPV++T LLFSDK+AA EA  GS + E+V SKN WPHVTLWT EG+AAKEAN 
Sbjct: 1048 GIFENKEVPVELTALLFSDKMAAFEARLGSIENERVISKNEWPHVTLWTREGVAAKEANA 1107

Query: 405  LPQLFSEGKAARIVIDPPVTGIGTVEFY 322
            LPQL SEGKA  + I+PP+   G V+F+
Sbjct: 1108 LPQLVSEGKATLVEINPPIIISGMVKFF 1135