BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00007731
         (1247 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAL26842.1|AF313452_1 thermal hysteresis protein STHP-64 [Sol...   179   2e-42
gb|AAD16138.1| DNA-binding protein 1, partial [Nicotiana tabacum]     153   1e-34
ref|XP_003518571.1| PREDICTED: WRKY transcription factor 1-like ...   124   4e-26
emb|CBI28412.3| unnamed protein product [Vitis vinifera]              124   4e-26
ref|XP_002272407.1| PREDICTED: probable WRKY transcription facto...   124   4e-26

>gb|AAL26842.1|AF313452_1 thermal hysteresis protein STHP-64 [Solanum dulcamara]
          Length = 591

 Score =  179 bits (453), Expect = 2e-42
 Identities = 113/287 (39%), Positives = 156/287 (54%), Gaps = 4/287 (1%)
 Frame = +1

Query: 337  SPPSVLHDQEEPDNMQQKQSSPEPVISQVSQESINDVVEPEKGLNNLKPVQSLDDVTNAS 516
            +P     D+   +N++QK   P+P     S+  + +           K +   D   N S
Sbjct: 3    TPKEEATDEVFSENLEQKPD-PDPA---TSKSELKETTSKSISGELQKRLSQCDKGINMS 58

Query: 517  QSNQDGSSISIAHETLPVIVYQRHSFSSATRTSASCQEGSSLSTIPEKELDNMLPTQSHG 696
            QSNQ+  S+S   E     V ++    S    S S Q    LS++P K+ D     +S G
Sbjct: 59   QSNQEAISLSRVQENQSENVQKQLGLKSEADASGSSQ----LSSLP-KDSD----AKSRG 109

Query: 697  TIIITSGASEHGTDLSTLPAKMPDTLQQSQEPDTYLSAPQSYQEKYSLSMKQEKNLEKLP 876
                 S +S     +S L     D+  Q Q  +  +   QS Q++ S  +K+EK L+KL 
Sbjct: 110  -----SESSVKDKVVSVLSGMASDSSDQMQTSNMEVFVSQSDQQRVSYQIKREKALDKLQ 164

Query: 877  LRRNPDTGVAALQYVEGSPRSNIPEKPTEDGYNWRKYGQKLVRGNQFVRSYYKCTFPNCP 1056
             RRNPDT V  L   +G   S  PEKP+EDGYNWRKYGQKLV+GN+F RSYYKCT+PNC 
Sbjct: 165  PRRNPDTSVHGLTSDQGMTLSKAPEKPSEDGYNWRKYGQKLVKGNEFTRSYYKCTYPNCL 224

Query: 1057 AKKQVERSHEGNITNINCRGNHEHPK----PQQSSQTSMPIQAIRPE 1185
            AKKQVERSH+G+IT+I+  G HEHP+    PQ S +  +P+Q I+ +
Sbjct: 225  AKKQVERSHDGHITDIHYIGKHEHPETLSVPQMSPELVLPLQMIQQD 271



 Score = 80.1 bits (196), Expect = 1e-12
 Identities = 90/350 (25%), Positives = 143/350 (40%), Gaps = 56/350 (16%)
 Frame = +1

Query: 256  DAISSDKLQQ-GEDPDAVFHESMSTQKRSPPSVLHDQEEPDNMQQKQSSPEPVISQVSQE 432
            DA  S +L    +D DA    S S+ K    SVL       + Q + S+ E  +SQ  Q+
Sbjct: 89   DASGSSQLSSLPKDSDAKSRGSESSVKDKVVSVLSGMASDSSDQMQTSNMEVFVSQSDQQ 148

Query: 433  SINDVVEPEKGLNNLKPVQSLDDVTNASQSNQDGSSISIAHETLPVIVYQRHSFSSA--- 603
             ++  ++ EK L+ L+P ++ D   +   S+Q G ++S A E      Y    +      
Sbjct: 149  RVSYQIKREKALDKLQPRRNPDTSVHGLTSDQ-GMTLSKAPEKPSEDGYNWRKYGQKLVK 207

Query: 604  ----TRTSASCQEGSSLSTIPEKELDNMLPTQSHGTIIITSGASEHGTDLSTLPAKMPDT 771
                TR+   C   + L+   +K+++       H T I   G  EH   LS +P   P+ 
Sbjct: 208  GNEFTRSYYKCTYPNCLA---KKQVERS--HDGHITDIHYIGKHEHPETLS-VPQMSPEL 261

Query: 772  ---LQQSQEPDTYLSAPQSYQEKYSL---------------------------------- 840
               LQ  Q+    ++A ++  EK +                                   
Sbjct: 262  VLPLQMIQQDIPIITALEAEGEKSTTPRETCEPSKPSEVPLALDVVSACGGVKVTPLKQH 321

Query: 841  SMKQEKNLEKLPLRRNPDTGVAALQYV-----EGSPRSNIPEKPTE-----DGYNWRKYG 990
             ++ E + + +P  +     + A  Y         PR +I +  +E     DG  W KYG
Sbjct: 322  KLENEVDKDDVPDSKRQKKDIVATDYTPPVKSHSEPR-HIVQTMSEVDIINDGQRWPKYG 380

Query: 991  QKLVRGNQFVRSYYKCTFPNCPAKKQVER-SHEGNITNINCRGNHEHPKP 1137
            QK+V+GN   RSYY+C+   CP KK VER SH+  +      G+H H  P
Sbjct: 381  QKIVKGNPNPRSYYRCSVAGCPVKKHVERASHDPKLVITTYEGHHVHDFP 430


>gb|AAD16138.1| DNA-binding protein 1, partial [Nicotiana tabacum]
          Length = 454

 Score =  153 bits (386), Expect = 1e-34
 Identities = 80/169 (47%), Positives = 102/169 (60%), Gaps = 4/169 (2%)
 Frame = +1

Query: 739  LSTLPAKMPDTLQQSQEPDTYLSAPQSYQEKYSLSMKQEKNLEKLPLRRNPDTGVAALQY 918
            +S L  K  ++  Q Q     +   +S Q+  +   + EK L+KL  RRNPDT    L  
Sbjct: 50   VSPLSNKASNSSDQIQSQTMEVVVSRSDQQLVTYPARPEKTLDKLQPRRNPDTSGHGLPS 109

Query: 919  VEGSPRSNIPEKPTEDGYNWRKYGQKLVRGNQFVRSYYKCTFPNCPAKKQVERSHEGNIT 1098
              G+    +PEKP EDGYNWRKYGQKLVRGN+F RSYYKCT+PNC AKKQVERSH+G+IT
Sbjct: 110  DRGNTLFKLPEKPLEDGYNWRKYGQKLVRGNEFTRSYYKCTYPNCLAKKQVERSHDGHIT 169

Query: 1099 NINCRGNHEHPKPQQSSQTS----MPIQAIRPEKSSLFGSEVTTSEDKT 1233
            +++  G HEHPK     QT     +P+Q  +P+   L  SE    E  T
Sbjct: 170  DVHYIGKHEHPKTPSGPQTPPGLVVPLQMRQPDIPMLTASEEAEGEKST 218



 Score = 67.4 bits (163), Expect = 7e-09
 Identities = 29/56 (51%), Positives = 36/56 (64%), Gaps = 1/56 (1%)
 Frame = +1

Query: 964  DGYNWRKYGQKLVRGNQFVRSYYKCTFPNCPAKKQVER-SHEGNITNINCRGNHEH 1128
            DG+ WRKYGQK V+GN   RSYY+C+   CP KK VER SH+  +      G H+H
Sbjct: 301  DGHRWRKYGQKFVKGNPNPRSYYRCSIAGCPVKKHVERASHDPKMVITTYEGQHDH 356


>ref|XP_003518571.1| PREDICTED: WRKY transcription factor 1-like [Glycine max]
          Length = 507

 Score =  124 bits (312), Expect = 4e-26
 Identities = 72/163 (44%), Positives = 98/163 (60%), Gaps = 6/163 (3%)
 Frame = +1

Query: 775  QQSQEPDTYLSAPQSYQEKYSLSMKQEKNLEKLPLRRNPDTGVAALQYVEGSPR--SNIP 948
            ++ ++ D    A +S QE  + S+  EK L+      NPDT    L  ++ S    S I 
Sbjct: 55   EEGKDSDAIACALESDQEGSTCSLPLEKPLQ------NPDTLSHELPRLQSSQEFPSIIR 108

Query: 949  EKPTEDGYNWRKYGQKLVRGNQFVRSYYKCTFPNCPAKKQVERSHEGNITNINCRGNHEH 1128
            EK ++DGYNWRKYGQK V+GN+F+RSYYKCT PNC AKKQ+++S+ G+IT+  C G H H
Sbjct: 109  EKVSKDGYNWRKYGQKHVKGNEFIRSYYKCTHPNCLAKKQLQQSNNGHITDSICIGQHNH 168

Query: 1129 PKPQQSSQTS----MPIQAIRPEKSSLFGSEVTTSEDKTTDVH 1245
            P+PQ +S  S    +P+    P KSSL      T EDK +  H
Sbjct: 169  PRPQLNSTVSVECVLPVVEQAPHKSSL-----ATVEDKASVEH 206



 Score = 85.1 bits (209), Expect = 3e-14
 Identities = 98/384 (25%), Positives = 152/384 (39%), Gaps = 23/384 (5%)
 Frame = +1

Query: 157  GEQLTDPIISPEFRSFESRMLKTQMINSKDGVPDAISSDKLQQG-----------EDPDA 303
            G    + +  PE  S  S ++K +     D +  A+ SD  Q+G           ++PD 
Sbjct: 32   GHDTKNDLSKPEEASILSIVVKNEEGKDSDAIACALESD--QEGSTCSLPLEKPLQNPDT 89

Query: 304  VFHESMSTQKRSP-PSVLHDQEEPDNMQQKQSSPEPVISQVSQESINDVVEP----EKGL 468
            + HE    Q     PS++ ++   D    ++   + V       S      P    +K L
Sbjct: 90   LSHELPRLQSSQEFPSIIREKVSKDGYNWRKYGQKHVKGNEFIRSYYKCTHPNCLAKKQL 149

Query: 469  NNLKPVQSLDDVTNASQSN---QDGSSISIAHETLPVIVYQRHSFSSAT-RTSASCQEGS 636
                     D +     ++   Q  S++S+    LPV+    H  S AT    AS + G 
Sbjct: 150  QQSNNGHITDSICIGQHNHPRPQLNSTVSVEC-VLPVVEQAPHKSSLATVEDKASVEHGC 208

Query: 637  SLSTIPEKELDNMLPTQSHGTIIITSGASEHGTDLSTLPAKMPDTLQQSQEPDTYLSAPQ 816
                +P++    + P QS     ++     + + LS   AK  + +  ++EP++      
Sbjct: 209  ----MPQQ----IQPLQSFPPAKVSPVNKLNASHLSLTKAK--NQVHDNEEPES------ 252

Query: 817  SYQEKYSLSMKQEKNLEKLPLRRNPDTGVAALQYVEGSP--RSNIPEKPTEDGYNWRKYG 990
                         K L+K     NPD     +   E     +++       DGY WRKYG
Sbjct: 253  -------------KRLKKD--NTNPDVTRVDMSTRESRVVVQTSSEVDLVNDGYRWRKYG 297

Query: 991  QKLVRGNQFVRSYYKCTFPNCPAKKQVER-SHEGNITNINCRGNHEHPKPQQSSQTSMPI 1167
            QKLV+GN   RSYY+C+ P CP KK VER SH+  +      G H+H  P   + T    
Sbjct: 298  QKLVKGNTNPRSYYRCSNPGCPVKKHVERASHDSKVVITTYEGQHDHEIPPGRTVTQNAA 357

Query: 1168 QAIRPEKSSLFGSEVTTSEDKTTD 1239
               R   S+  G   T SE   TD
Sbjct: 358  TNARTTASN--GKAGTKSEGNITD 379


>emb|CBI28412.3| unnamed protein product [Vitis vinifera]
          Length = 364

 Score =  124 bits (312), Expect = 4e-26
 Identities = 67/129 (51%), Positives = 85/129 (65%), Gaps = 5/129 (3%)
 Frame = +1

Query: 868  KLPLRRNPDTGVAALQ-YVEGSPRSNIPEKPTEDGYNWRKYGQKLVRGNQFVRSYYKCTF 1044
            KL  R+ PDTGV A Q + E    S IPEK +EDGYNWRKYGQK V+GN+F+RSYY+CT 
Sbjct: 35   KLQQRQIPDTGVHASQSHQEAIMPSIIPEKASEDGYNWRKYGQKHVKGNEFIRSYYRCTH 94

Query: 1045 PNCPAKKQVERSHEGNITNINCRGNHEHPKPQQSSQTS----MPIQAIRPEKSSLFGSEV 1212
            PNC  KKQ+ERSH+G IT+I   G H+HPK Q     +    +P+Q  RP++ S      
Sbjct: 95   PNCQVKKQLERSHDGQITDIIYFGKHDHPKLQVDLPLAVGLVVPVQEERPKEPS-----S 149

Query: 1213 TTSEDKTTD 1239
            T  E+K+ D
Sbjct: 150  TVVEEKSLD 158



 Score = 73.6 bits (179), Expect = 1e-10
 Identities = 73/304 (24%), Positives = 118/304 (38%), Gaps = 6/304 (1%)
 Frame = +1

Query: 262  ISSDKLQQGEDPDAVFHESMSTQKRSPPSVLHDQEEPDNMQQKQSSPEPVISQVSQESIN 441
            + S+KLQQ + PD   H S S Q+   PS++ ++   D    ++   + V       S  
Sbjct: 31   VDSNKLQQRQIPDTGVHASQSHQEAIMPSIIPEKASEDGYNWRKYGQKHVKGNEFIRSYY 90

Query: 442  DVVEP----EKGLNNLKPVQSLDDVTNASQSNQDGS-SISIAHETLPVIVYQRHSFSSAT 606
                P    +K L      Q  D +      +      + +A   +  +  +R    S+T
Sbjct: 91   RCTHPNCQVKKQLERSHDGQITDIIYFGKHDHPKLQVDLPLAVGLVVPVQEERPKEPSST 150

Query: 607  RTSASCQEGSSLSTIPEKELDNMLPTQSHGTIIITSGASEHGTDLSTLPAKMPDTLQQSQ 786
                   +G   ++   + +D   P      I ++    +       + ++  D      
Sbjct: 151  VVEEKSLDGDGQTSCQIEPVDAPQPA-----IAVSDDCVDRAL---AVWSRTRDETDNDD 202

Query: 787  EPDTYLSAPQSYQEKYSLSMKQEKNLEKLPLRRNPDTGVAALQYVEGSPRSNIPEKPTED 966
            +PD       S ++K     K   N++  P  +        +Q V      N       D
Sbjct: 203  DPD-------SKRQK-----KDINNVDATPTDKPSGEPRIVVQTVSEVDIVN-------D 243

Query: 967  GYNWRKYGQKLVRGNQFVRSYYKCTFPNCPAKKQVER-SHEGNITNINCRGNHEHPKPQQ 1143
            GY WRKYGQKLV+GN   RSYY+C+   CP KK VER SH+  +      G H+H  P  
Sbjct: 244  GYRWRKYGQKLVKGNTNPRSYYRCSNAGCPVKKHVERASHDPKMVITTYEGQHDHDMPPA 303

Query: 1144 SSQT 1155
             + T
Sbjct: 304  RTVT 307


>ref|XP_002272407.1| PREDICTED: probable WRKY transcription factor 20-like [Vitis
            vinifera]
          Length = 407

 Score =  124 bits (312), Expect = 4e-26
 Identities = 67/129 (51%), Positives = 85/129 (65%), Gaps = 5/129 (3%)
 Frame = +1

Query: 868  KLPLRRNPDTGVAALQ-YVEGSPRSNIPEKPTEDGYNWRKYGQKLVRGNQFVRSYYKCTF 1044
            KL  R+ PDTGV A Q + E    S IPEK +EDGYNWRKYGQK V+GN+F+RSYY+CT 
Sbjct: 78   KLQQRQIPDTGVHASQSHQEAIMPSIIPEKASEDGYNWRKYGQKHVKGNEFIRSYYRCTH 137

Query: 1045 PNCPAKKQVERSHEGNITNINCRGNHEHPKPQQSSQTS----MPIQAIRPEKSSLFGSEV 1212
            PNC  KKQ+ERSH+G IT+I   G H+HPK Q     +    +P+Q  RP++ S      
Sbjct: 138  PNCQVKKQLERSHDGQITDIIYFGKHDHPKLQVDLPLAVGLVVPVQEERPKEPS-----S 192

Query: 1213 TTSEDKTTD 1239
            T  E+K+ D
Sbjct: 193  TVVEEKSLD 201



 Score = 87.4 bits (215), Expect = 7e-15
 Identities = 94/354 (26%), Positives = 132/354 (37%), Gaps = 45/354 (12%)
 Frame = +1

Query: 229  MINSKDGVPDAISSDKLQQGEDPDAVFHESMSTQKRSPPSVLHDQEEPDNMQQKQSSPEP 408
            M  S +GV D ++SDKLQQ +  +A  + S S Q+ + P +     E +      +S   
Sbjct: 1    MGTSMEGVQDDVASDKLQQRQSLNAGVNASQSVQEVTDPIIPEKASEAELNPSFAASSSQ 60

Query: 409  VISQVSQESINDVVEPEKGLNNLKPVQSLDDVTNASQSNQDGSSISIAHETLPVIVYQRH 588
            V    S E   D V+     N L+  Q  D   +ASQS+Q+    SI  E      Y   
Sbjct: 61   VDMGTSGEGAADDVDS----NKLQQRQIPDTGVHASQSHQEAIMPSIIPEKASEDGYNWR 116

Query: 589  SFSSATRTSASCQEGSSLSTIPEKELDNMLPTQSHGTI--IITSGASEHGTDLSTLPAKM 762
             +                 T P  ++   L     G I  II  G  +H      LP  +
Sbjct: 117  KYGQKHVKGNEFIRSYYRCTHPNCQVKKQLERSHDGQITDIIYFGKHDHPKLQVDLPLAV 176

Query: 763  ----PDTLQQSQEPDTYLSAPQSYQEKYSLSMKQEKNLEKLPLRRNPDTGVAALQYVEGS 930
                P   ++ +EP + +   +S       S + E      P     D  V     V   
Sbjct: 177  GLVVPVQEERPKEPSSTVVEEKSLDGDGQTSCQIEPVDAPQPAIAVSDDCVDRALAVWSR 236

Query: 931  PR------------------SNIPEKPTE--------------------DGYNWRKYGQK 996
             R                  +N+   PT+                    DGY WRKYGQK
Sbjct: 237  TRDETDNDDDPDSKRQKKDINNVDATPTDKPSGEPRIVVQTVSEVDIVNDGYRWRKYGQK 296

Query: 997  LVRGNQFVRSYYKCTFPNCPAKKQVER-SHEGNITNINCRGNHEHPKPQQSSQT 1155
            LV+GN   RSYY+C+   CP KK VER SH+  +      G H+H  P   + T
Sbjct: 297  LVKGNTNPRSYYRCSNAGCPVKKHVERASHDPKMVITTYEGQHDHDMPPARTVT 350