BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00007554
         (1289 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002277399.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   382   e-103
ref|XP_003540274.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   382   e-103
gb|ACU19018.1| unknown [Glycine max]                                  378   e-102
ref|XP_003543397.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   376   e-102
ref|XP_002322768.1| predicted protein [Populus trichocarpa] gi|2...   367   3e-99

>ref|XP_002277399.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A [Vitis vinifera]
            gi|296084011|emb|CBI24399.3| unnamed protein product
            [Vitis vinifera]
          Length = 393

 Score =  382 bits (981), Expect = e-103
 Identities = 208/390 (53%), Positives = 245/390 (62%), Gaps = 2/390 (0%)
 Frame = +3

Query: 90   PRMEDDKKAENHLTSAAAFVEGGVQDACDDACSICLEVFSESEPSTVTVCKHEFHLQCIL 269
            P ME+  K+E HLTSAAAFVEGG+Q+ACDDACSICLE F +S+PSTVT CKHEFHLQCIL
Sbjct: 4    PGMEESNKSECHLTSAAAFVEGGIQEACDDACSICLEAFCDSDPSTVTSCKHEFHLQCIL 63

Query: 270  EWCQRSSQCPMCWQPISLKDPSSQELLDAIERERSIRATPSRNTAIFHHPTLGDFELQHL 449
            EWCQRSSQCPMCWQPISLKDP+SQELL+A+ERER+ R  PSRN  IFHHPTLGDFELQHL
Sbjct: 64   EWCQRSSQCPMCWQPISLKDPTSQELLEAVERERTFRFNPSRNATIFHHPTLGDFELQHL 123

Query: 450  PVGATDAELEDRIIQHLXXXXXXXXXXXXXXXEGQRNRSSAQGRPHFLVF-XXXXXXXXX 626
            PVGA DAELE+RIIQHL               EGQR RSSAQGRP FLVF          
Sbjct: 124  PVGANDAELEERIIQHLAAAAAMGRARHIARREGQRTRSSAQGRPQFLVFSAHPSTPQAG 183

Query: 627  XXXXXXXQRGEGENDPYIIESGSTPLQLGMNEESVQPNAPPLSVQSIQASGS-XXXXXXX 803
                   QRGE E  P I  +  + L   + EES +     LS Q+ + + S        
Sbjct: 184  PASSSPAQRGEDEPAPAITAAMPSSLPTAVGEESSRLTTSQLSSQAEEITASASGSIIHT 243

Query: 804  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLKSRFNAMSSKYKESITKSTKGWK 983
                                                LKSRFNA+S +YKESI+KST+GWK
Sbjct: 244  TNQHGTSSNNRRSPGQSLPSGQDKAGPSEFQSFSETLKSRFNAVSMRYKESISKSTRGWK 303

Query: 984  EKLFSRNSSATDSGSEVRREVNVDVSTVSRMMGNLETNDNIPPPSISVSDTLEGRSVLEN 1163
            E+LFSRN+S  D GS+VRREVN  ++TVSRMM +LET DN      SVSD+ E  S    
Sbjct: 304  ERLFSRNTSMADIGSDVRREVNAGIATVSRMMEHLETRDNSRTTPSSVSDSSEDTSSPSL 363

Query: 1164 GNQQTSRNDGNTSLNERNVQASCAATSASD 1253
            G+Q+ +    +  L + NVQASCAA+S ++
Sbjct: 364  GDQRMAETSAHNPLGDTNVQASCAASSGTN 393


>ref|XP_003540274.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A-like [Glycine max]
          Length = 401

 Score =  382 bits (980), Expect = e-103
 Identities = 206/387 (53%), Positives = 246/387 (63%), Gaps = 2/387 (0%)
 Frame = +3

Query: 99   EDDKKAENHLTSAAAFVEGGVQDACDDACSICLEVFSESEPSTVTVCKHEFHLQCILEWC 278
            +++ K+E HLTSAAAFVEGG+QDACDDACSICLE F +S+PSTVT CKHEFHLQCILEWC
Sbjct: 16   DNNNKSEAHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVTSCKHEFHLQCILEWC 75

Query: 279  QRSSQCPMCWQPISLKDPSSQELLDAIERERSIRATPSRNTAIFHHPTLGDFELQHLPVG 458
            QRSSQCPMCWQPISLKDP+SQELL+A+ERER+ R  PSRN  IFHHPTLGDFELQHLPVG
Sbjct: 76   QRSSQCPMCWQPISLKDPTSQELLEAVERERNFRFNPSRNATIFHHPTLGDFELQHLPVG 135

Query: 459  ATDAELEDRIIQHLXXXXXXXXXXXXXXXEGQRNRSSAQGRPHFLVF-XXXXXXXXXXXX 635
            A+DA+LE+RIIQHL               EGQRNRSSAQGRPHFLVF             
Sbjct: 136  ASDADLEERIIQHLAAAAAMGRARHIARREGQRNRSSAQGRPHFLVFSTNPNSPPMAPAS 195

Query: 636  XXXXQRGEGENDPYIIESGSTPLQLGMNEESVQPNAPPLSVQSIQASGSXXXXXXXXXXX 815
                QRG+ E  P I  +  +P      E S     PP+  + + ASGS           
Sbjct: 196  SSPSQRGDDEPIPTIAVANLSPTPATGEESSQLTLVPPIQAEQVSASGS-GSTALATDHQ 254

Query: 816  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLKSRFNAMSSKYKESITKSTKGWKEKLF 995
                                            LKS+ NA+S++YKESI+KST+GWKE+ F
Sbjct: 255  GSSYNNRRSPNQSSPSSQDRAGPSELQSFSESLKSKLNAVSTRYKESISKSTRGWKERWF 314

Query: 996  SRNSSATDSGSEVRREVNVDVSTVSRMMGNLETNDNIPPPSISVSDTLEGRSVLE-NGNQ 1172
            SRN++ +D GSEVRREVN  ++TVSRMM  LET+DN    S S    +E  SV E NG  
Sbjct: 315  SRNNTMSDLGSEVRREVNAGIATVSRMMERLETSDNNRSNSNSAPSNIEDGSVQEYNGQT 374

Query: 1173 QTSRNDGNTSLNERNVQASCAATSASD 1253
             T  N+G+  L + N +ASCAA S+S+
Sbjct: 375  LTPGNEGDGLLRDNNTKASCAAGSSSN 401


>gb|ACU19018.1| unknown [Glycine max]
          Length = 401

 Score =  378 bits (970), Expect = e-102
 Identities = 204/387 (52%), Positives = 244/387 (63%), Gaps = 2/387 (0%)
 Frame = +3

Query: 99   EDDKKAENHLTSAAAFVEGGVQDACDDACSICLEVFSESEPSTVTVCKHEFHLQCILEWC 278
            +++ K+E HLTSAAAFVEGG+QDACDDACSICLE F +S+PSTVT CKHEFHLQCILEWC
Sbjct: 16   DNNNKSEAHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVTSCKHEFHLQCILEWC 75

Query: 279  QRSSQCPMCWQPISLKDPSSQELLDAIERERSIRATPSRNTAIFHHPTLGDFELQHLPVG 458
            QRSSQCPMCWQPISLKDP+SQELL+A+ERER+ R  PSRN  IFHHPTLGDFELQHLPVG
Sbjct: 76   QRSSQCPMCWQPISLKDPTSQELLEAVERERNFRFNPSRNATIFHHPTLGDFELQHLPVG 135

Query: 459  ATDAELEDRIIQHLXXXXXXXXXXXXXXXEGQRNRSSAQGRPHFLVF-XXXXXXXXXXXX 635
            A+DA+LE+RIIQHL               EGQRNRSSAQGRPHFL F             
Sbjct: 136  ASDADLEERIIQHLAAAAAMGRARHIARREGQRNRSSAQGRPHFLAFSTNPNSPPMAPAS 195

Query: 636  XXXXQRGEGENDPYIIESGSTPLQLGMNEESVQPNAPPLSVQSIQASGSXXXXXXXXXXX 815
                QRG+ E  P I  +  +P      E S     PP+  + + ASGS           
Sbjct: 196  SSPSQRGDDEPIPTIAVANLSPTPATGEESSQLTLVPPIQAEQVSASGS-GSTALATDHQ 254

Query: 816  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLKSRFNAMSSKYKESITKSTKGWKEKLF 995
                                            LKS+ NA+S++YKESI+KST+GWKE+ F
Sbjct: 255  GSSYNNRRSPNQSSPSSQDRAGPSELQSFSESLKSKLNAVSTRYKESISKSTRGWKERWF 314

Query: 996  SRNSSATDSGSEVRREVNVDVSTVSRMMGNLETNDNIPPPSISVSDTLEGRSVLE-NGNQ 1172
            SRN++ +D GSEVRREVN  ++TVSRMM  LET+DN    S S    +E  SV E NG  
Sbjct: 315  SRNNTMSDLGSEVRREVNAGIATVSRMMERLETSDNNRSNSNSAPSNIEDGSVQEYNGQT 374

Query: 1173 QTSRNDGNTSLNERNVQASCAATSASD 1253
             T  N+G+  L + N +ASCAA  +S+
Sbjct: 375  LTPGNEGDGLLRDNNTKASCAAGFSSN 401


>ref|XP_003543397.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A-like [Glycine max]
          Length = 398

 Score =  376 bits (966), Expect = e-102
 Identities = 206/389 (52%), Positives = 247/389 (63%), Gaps = 3/389 (0%)
 Frame = +3

Query: 96   MEDDKKAENHLTSAAAFVEGGVQDACDDACSICLEVFSESEPSTVTVCKHEFHLQCILEW 275
            + DD K+E HLTSAAAFVEGG+QDACDDACSICLE F +S+PSTVT CKHEFHLQCILEW
Sbjct: 12   INDDNKSEAHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVTSCKHEFHLQCILEW 71

Query: 276  CQRSSQCPMCWQPISLKDPSSQELLDAIERERSIRATPSRNTAIFHHPTLGDFELQHLPV 455
            CQRSSQCPMCWQPISLKDP+SQELL+A+ERER+ R  PSRN  IFHHPTLGDFELQHLPV
Sbjct: 72   CQRSSQCPMCWQPISLKDPTSQELLEAVERERNFRFNPSRNATIFHHPTLGDFELQHLPV 131

Query: 456  GATDAELEDRIIQHLXXXXXXXXXXXXXXXEGQRNRSSAQGRPHFLVF-XXXXXXXXXXX 632
            GA+DA+LE+RIIQHL               EGQRNRSSAQGR HFLVF            
Sbjct: 132  GASDADLEERIIQHLAAAAAMGRARHVARREGQRNRSSAQGRQHFLVFSTHPNSPPMAPA 191

Query: 633  XXXXXQRGEGENDPYIIESGSTPLQLGMNEESVQPN-APPLSVQSIQASGSXXXXXXXXX 809
                 QRG+ E  P I  +  +P      EES QP    P+  + + ASGS         
Sbjct: 192  SSSPSQRGDDEPTPTIDVANLSPTP-ATGEESSQPTLVSPVQAEQVSASGS-GTTALATD 249

Query: 810  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLKSRFNAMSSKYKESITKSTKGWKEK 989
                                              LKS+ NA+S++YKESI+KST+GWKE+
Sbjct: 250  HQGSSYNNRRSPNQSSPSSQDRAGPSELQSFSESLKSKLNAVSTRYKESISKSTRGWKER 309

Query: 990  LFSRNSSATDSGSEVRREVNVDVSTVSRMMGNLETNDNIPPPSISVSDTLEGRSVLENGN 1169
             FSRN++ +D GSEVRREVN  ++TVSRMM  LET DN    S S    +E  SV  + +
Sbjct: 310  WFSRNNTMSDLGSEVRREVNAGIATVSRMMERLETRDNNRSNSNSAPSNIEDGSVQGSND 369

Query: 1170 QQ-TSRNDGNTSLNERNVQASCAATSASD 1253
            Q  T+ N+G + + + N +ASCAA S+S+
Sbjct: 370  QTLTAGNEGGSLVRDNNTKASCAAGSSSN 398


>ref|XP_002322768.1| predicted protein [Populus trichocarpa] gi|222867398|gb|EEF04529.1|
            predicted protein [Populus trichocarpa]
          Length = 387

 Score =  367 bits (943), Expect = 3e-99
 Identities = 199/386 (51%), Positives = 241/386 (62%), Gaps = 1/386 (0%)
 Frame = +3

Query: 96   MEDDKKAENHLTSAAAFVEGGVQDACDDACSICLEVFSESEPSTVTVCKHEFHLQCILEW 275
            M++ KK E H+TSAAAFVEGG+QD+CDDACSICLE F ES+PSTVT CKHEFHLQCILEW
Sbjct: 1    MDEAKKPEVHMTSAAAFVEGGIQDSCDDACSICLEEFCESDPSTVTNCKHEFHLQCILEW 60

Query: 276  CQRSSQCPMCWQPISLKDPSSQELLDAIERERSIRATPSRNTAIFHHPTLGDFELQHLPV 455
            CQRSS+CPMC Q ISLKDP+SQEL +A+ERER IRA PSRN  IFHHPTLGDFELQHLPV
Sbjct: 61   CQRSSECPMCLQSISLKDPTSQELFEAVERERKIRAAPSRNATIFHHPTLGDFELQHLPV 120

Query: 456  GATDAELEDRIIQHLXXXXXXXXXXXXXXXEGQRNRSSAQGRPHFLVFXXXXXXXXXXXX 635
            G +D+ELE+RIIQHL               EGQRNR+S+ GRPHFLVF            
Sbjct: 121  GVSDSELEERIIQHLAAAAAMGRTRHFGRREGQRNRTSSHGRPHFLVFSTHPGAQPSGHV 180

Query: 636  XXXXQRGEGENDPYIIESGSTPLQL-GMNEESVQPNAPPLSVQSIQASGSXXXXXXXXXX 812
                 +  GEN+P  +   S   QL  + +E  Q      SV + Q+S +          
Sbjct: 181  SSSLTQLGGENEPAPVSVASPSTQLTSVGDEPPQQALQSPSVLTDQSSSASGSTAMPANN 240

Query: 813  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLKSRFNAMSSKYKESITKSTKGWKEKL 992
                                             LKSR NA+S +YKESI++ST+GWKE+L
Sbjct: 241  QGVSFNNRSASSQSSLPNQDRAGPSELQSFSESLKSRLNAVSMRYKESISRSTRGWKERL 300

Query: 993  FSRNSSATDSGSEVRREVNVDVSTVSRMMGNLETNDNIPPPSISVSDTLEGRSVLENGNQ 1172
            FSRNSS +D  S+VRREVN  ++TVSRMM  LET D+     +SVS+ L  RS +E  NQ
Sbjct: 301  FSRNSSMSDISSDVRREVNAGIATVSRMMERLETRDDGGDNQVSVSNHLTDRSNVERSNQ 360

Query: 1173 QTSRNDGNTSLNERNVQASCAATSAS 1250
             T+     + LNE +  A+CAA+SAS
Sbjct: 361  NTAATHIESPLNEGSTPAACAASSAS 386