BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00007513
         (1361 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003523834.1| PREDICTED: DUF21 domain-containing protein A...   493   e-158
ref|XP_004147782.1| PREDICTED: DUF21 domain-containing protein A...   477   e-152
ref|XP_004154837.1| PREDICTED: LOW QUALITY PROTEIN: DUF21 domain...   473   e-150
ref|XP_002282587.1| PREDICTED: DUF21 domain-containing protein A...   525   e-146
emb|CBI19695.3| unnamed protein product [Vitis vinifera]              525   e-146

>ref|XP_003523834.1| PREDICTED: DUF21 domain-containing protein At1g47330 [Glycine max]
          Length = 487

 Score =  493 bits (1268), Expect(2) = e-158
 Identities = 257/364 (70%), Positives = 290/364 (79%), Gaps = 17/364 (4%)
 Frame = -2

Query: 1291 MAADIPCCGTQFALYILVSIGLVXXXXXXXXXXXXXXXXXXXXLEVLIKSGRPQDRKHAA 1112
            MAA+IPCCGT+F+LY+LV IGLV                    LEVLIKSGRPQDR HA+
Sbjct: 1    MAAEIPCCGTKFSLYVLVIIGLVCFAGLMAGLTLGLMSLGLVDLEVLIKSGRPQDRIHAS 60

Query: 1111 KIFPVVKNQHLLLCTLLIGNSLAMESLPIFLDKLLPPWAAILLSVTLILMFAEILPQAIC 932
            KI+PVVKNQHLLLCTLLIGNSLAME+LPIFLD L+ P AAIL+SVTLILMF EILPQAIC
Sbjct: 61   KIYPVVKNQHLLLCTLLIGNSLAMEALPIFLDSLVHPAAAILISVTLILMFGEILPQAIC 120

Query: 931  TRYGLTVGATVAPFVQLLLWLFFPIAYPISKVLDWMLGKGHAALLRRAELKTFVDFHGNE 752
            TRYGLTVGAT+AP V++LL +FFP++YPISKVLDWMLGKGHAALL+RAELKTFV+FHGNE
Sbjct: 121  TRYGLTVGATLAPLVRVLLIVFFPLSYPISKVLDWMLGKGHAALLKRAELKTFVNFHGNE 180

Query: 751  AGKGGDLTHDETTIIAGALELTEKTVKDAMTPISKAFSIDLDGTLSLETLNAIMTMGHSR 572
            AGKGGDLTHDETTII GAL+LTEKT KDAMTPISKAFS+DLD TL+LETLN+IMT+GHSR
Sbjct: 181  AGKGGDLTHDETTIITGALDLTEKTAKDAMTPISKAFSLDLDATLNLETLNSIMTIGHSR 240

Query: 571  VPVYCGKPTNIVGIILVKNLLTVDPVDAVPLRKMLIRKVPRVSEDMPLYDILNEFQKGHS 392
            VPVY G+ TNI+G++LVKNL  VD   AVPLRKMLIRK+PRVSE+MPLYDILNEFQKGHS
Sbjct: 241  VPVYAGEKTNIIGLVLVKNLFMVDSKAAVPLRKMLIRKIPRVSENMPLYDILNEFQKGHS 300

Query: 391  HIAVVYKDLNGTNEFSKKTND----------------ATQNMASKARSHAADRIDG-NEN 263
            HIAVVY+DLN  NE  KK  D                 + +   K  SH +   DG  + 
Sbjct: 301  HIAVVYRDLNDKNEAPKKVKDGELLDLKDKRKNKGEKTSLDKGEKLESHYSLTTDGAQQA 360

Query: 262  KKSP 251
            KKSP
Sbjct: 361  KKSP 364



 Score = 95.5 bits (236), Expect(2) = e-158
 Identities = 44/52 (84%), Positives = 49/52 (94%)
 Frame = -1

Query: 158 AAPAFKKRHKGCSFCILDLDNAPVPEFPSNEVVVGVISMEDVIEELLQEEIL 3
           A PAFKKRH+GCS+CILDLDN+P+P FP NEVVVGVI+MEDVIEELLQEEIL
Sbjct: 366 ATPAFKKRHRGCSYCILDLDNSPLPVFPPNEVVVGVITMEDVIEELLQEEIL 417


>ref|XP_004147782.1| PREDICTED: DUF21 domain-containing protein At1g47330-like [Cucumis
            sativus]
          Length = 449

 Score =  477 bits (1228), Expect(2) = e-152
 Identities = 249/359 (69%), Positives = 280/359 (77%), Gaps = 12/359 (3%)
 Frame = -2

Query: 1291 MAADIPCCGTQFALYILVSIGLVXXXXXXXXXXXXXXXXXXXXLEVLIKSGRPQDRKHAA 1112
            MA D+ CC ++F L++L+  GLV                    LEVLIKSGRPQDRKHAA
Sbjct: 1    MAEDVRCCESKFFLFLLIIAGLVAFAGLMAGLTLGLMSLGLVDLEVLIKSGRPQDRKHAA 60

Query: 1111 KIFPVVKNQHLLLCTLLIGNSLAMESLPIFLDKLLPPWAAILLSVTLILMFAEILPQAIC 932
            KI PVVKNQHLLLCTLLIGNSLAME+LPIFLD ++PPWAA+L+SVTLILMF EILPQAIC
Sbjct: 61   KILPVVKNQHLLLCTLLIGNSLAMEALPIFLDMIVPPWAAVLVSVTLILMFGEILPQAIC 120

Query: 931  TRYGLTVGATVAPFVQLLLWLFFPIAYPISKVLDWMLGKGHAALLRRAELKTFVDFHGNE 752
            TRYGL VGA +AP V++LL +FFPI+YPISKVLDWMLGKGHA LLRRAELKTFV+FHGNE
Sbjct: 121  TRYGLKVGAIMAPLVRILLIVFFPISYPISKVLDWMLGKGHAVLLRRAELKTFVNFHGNE 180

Query: 751  AGKGGDLTHDETTIIAGALELTEKTVKDAMTPISKAFSIDLDGTLSLETLNAIMTMGHSR 572
            AGKGGDLTHDETTIIAGALELTEKT KDAMT IS AFS+DLD TL LETLNAIMT GHSR
Sbjct: 181  AGKGGDLTHDETTIIAGALELTEKTAKDAMTSISNAFSLDLDATLDLETLNAIMTKGHSR 240

Query: 571  VPVYCGKPTNIVGIILVKNLLTVDPVDAVPLRKMLIRKVPRVSEDMPLYDILNEFQKGHS 392
            VPVY G P NI+G++LVKNLLTVDP D V L+KM+IRK+PRVSEDMPLYDILNEFQKGHS
Sbjct: 241  VPVYSGDPKNIIGLVLVKNLLTVDPEDRVSLKKMIIRKIPRVSEDMPLYDILNEFQKGHS 300

Query: 391  HIAVVYKDLNGTNEFSKKTN--------DATQNMASKARS----HAADRIDGNENKKSP 251
            HIAVV+K     +E   K +         A QN+  K  S      A++  G + KKSP
Sbjct: 301  HIAVVFKKHGHQSETLPKKDIGVNSGDAAAAQNIGMKMESVDAQTVAEKAGGLQTKKSP 359



 Score = 90.1 bits (222), Expect(2) = e-152
 Identities = 42/52 (80%), Positives = 47/52 (90%)
 Frame = -1

Query: 158 AAPAFKKRHKGCSFCILDLDNAPVPEFPSNEVVVGVISMEDVIEELLQEEIL 3
           A PAFKKRH+GCSFCILD++NAP+P FP  E VVGVI+MEDVIEELLQEEIL
Sbjct: 361 ATPAFKKRHRGCSFCILDVENAPLPVFPLGEEVVGVITMEDVIEELLQEEIL 412


>ref|XP_004154837.1| PREDICTED: LOW QUALITY PROTEIN: DUF21 domain-containing protein
            At1g47330-like [Cucumis sativus]
          Length = 449

 Score =  473 bits (1217), Expect(2) = e-150
 Identities = 247/359 (68%), Positives = 278/359 (77%), Gaps = 12/359 (3%)
 Frame = -2

Query: 1291 MAADIPCCGTQFALYILVSIGLVXXXXXXXXXXXXXXXXXXXXLEVLIKSGRPQDRKHAA 1112
            MA D+ CC ++F  ++L+  GLV                    LEVLIKSGRPQDRKHAA
Sbjct: 1    MAEDVRCCESKFFXFLLIIAGLVAFAGLMAGLTLGLMSLGLVDLEVLIKSGRPQDRKHAA 60

Query: 1111 KIFPVVKNQHLLLCTLLIGNSLAMESLPIFLDKLLPPWAAILLSVTLILMFAEILPQAIC 932
            KI PVVKNQHLLLCTLLIGNSLAME+LPIFLD ++PPWAA+L+SVTLILMF EILPQAIC
Sbjct: 61   KILPVVKNQHLLLCTLLIGNSLAMEALPIFLDMIVPPWAAVLVSVTLILMFGEILPQAIC 120

Query: 931  TRYGLTVGATVAPFVQLLLWLFFPIAYPISKVLDWMLGKGHAALLRRAELKTFVDFHGNE 752
            TRYGL VGA +AP V++LL +FFPI+YPISKVLDWMLGKGHA LLRRAELKTFV+FHGNE
Sbjct: 121  TRYGLKVGAIMAPLVRILLIVFFPISYPISKVLDWMLGKGHAVLLRRAELKTFVNFHGNE 180

Query: 751  AGKGGDLTHDETTIIAGALELTEKTVKDAMTPISKAFSIDLDGTLSLETLNAIMTMGHSR 572
            AGKGGDLTHDETTIIAGALELTEKT KDAMT IS AFS+DLD TL LETLNAIMT GHSR
Sbjct: 181  AGKGGDLTHDETTIIAGALELTEKTAKDAMTSISNAFSLDLDATLDLETLNAIMTKGHSR 240

Query: 571  VPVYCGKPTNIVGIILVKNLLTVDPVDAVPLRKMLIRKVPRVSEDMPLYDILNEFQKGHS 392
            VPVY G P NI+G++LVKNLLTVDP D V L+ M+IRK+PRVSEDMPLYDILNEFQKGHS
Sbjct: 241  VPVYSGDPKNIIGLVLVKNLLTVDPEDRVSLKXMIIRKIPRVSEDMPLYDILNEFQKGHS 300

Query: 391  HIAVVYKDLNGTNEFSKKTN--------DATQNMASKARS----HAADRIDGNENKKSP 251
            HIAVV+K     +E   K +         A QN+  K  S      A++  G + KKSP
Sbjct: 301  HIAVVFKKHGHQSETLPKKDIGVNSGDAAAAQNIGMKMESVDAQTVAEKAGGLQTKKSP 359



 Score = 88.6 bits (218), Expect(2) = e-150
 Identities = 41/52 (78%), Positives = 46/52 (88%)
 Frame = -1

Query: 158 AAPAFKKRHKGCSFCILDLDNAPVPEFPSNEVVVGVISMEDVIEELLQEEIL 3
           A PAFKKRH+GCSFCILD++N P+P FP  E VVGVI+MEDVIEELLQEEIL
Sbjct: 361 ATPAFKKRHRGCSFCILDVENVPLPVFPLGEEVVGVITMEDVIEELLQEEIL 412


>ref|XP_002282587.1| PREDICTED: DUF21 domain-containing protein At1g47330-like [Vitis
            vinifera]
          Length = 526

 Score =  525 bits (1352), Expect = e-146
 Identities = 262/329 (79%), Positives = 288/329 (87%)
 Frame = -2

Query: 1291 MAADIPCCGTQFALYILVSIGLVXXXXXXXXXXXXXXXXXXXXLEVLIKSGRPQDRKHAA 1112
            MAAD+ CCGT+F +Y+++ +GLV                    LEVLIKSGRPQDR HAA
Sbjct: 1    MAADVACCGTKFFIYVVIIVGLVGFAGLMAGLTLGLMSLGLVDLEVLIKSGRPQDRIHAA 60

Query: 1111 KIFPVVKNQHLLLCTLLIGNSLAMESLPIFLDKLLPPWAAILLSVTLILMFAEILPQAIC 932
            KIFPVVKNQHLLLCTLLIGNSLAMESLPIFLDKL+PPWAAIL+SVTLILMF EILPQA+C
Sbjct: 61   KIFPVVKNQHLLLCTLLIGNSLAMESLPIFLDKLVPPWAAILISVTLILMFGEILPQALC 120

Query: 931  TRYGLTVGATVAPFVQLLLWLFFPIAYPISKVLDWMLGKGHAALLRRAELKTFVDFHGNE 752
            TRYG+TVGAT+APFV++LL LF+PIAYPISKVLDWMLGKGHAALLRRAELKTFVDFHGNE
Sbjct: 121  TRYGMTVGATMAPFVRVLLLLFYPIAYPISKVLDWMLGKGHAALLRRAELKTFVDFHGNE 180

Query: 751  AGKGGDLTHDETTIIAGALELTEKTVKDAMTPISKAFSIDLDGTLSLETLNAIMTMGHSR 572
            AGKGGDLTHDETTIIAGALELTEKT KDAMTPISKAFS+DLDGTL+LETLNAIMT+GHSR
Sbjct: 181  AGKGGDLTHDETTIIAGALELTEKTAKDAMTPISKAFSLDLDGTLTLETLNAIMTIGHSR 240

Query: 571  VPVYCGKPTNIVGIILVKNLLTVDPVDAVPLRKMLIRKVPRVSEDMPLYDILNEFQKGHS 392
            VPVY GKPTNI+G+ILVKNLL VDP DAVPLRKM+IRK+PRVSE+MPLYDILNEFQKGHS
Sbjct: 241  VPVYAGKPTNIIGLILVKNLLMVDPDDAVPLRKMVIRKIPRVSENMPLYDILNEFQKGHS 300

Query: 391  HIAVVYKDLNGTNEFSKKTNDATQNMASK 305
            HIAVV+KDLN T E   KT D    ++ K
Sbjct: 301  HIAVVFKDLNETKEAQNKTKDGALQVSMK 329



 Score = 93.6 bits (231), Expect = 1e-16
 Identities = 42/52 (80%), Positives = 48/52 (92%)
 Frame = -1

Query: 158 AAPAFKKRHKGCSFCILDLDNAPVPEFPSNEVVVGVISMEDVIEELLQEEIL 3
           + P FKKRH+GCSFCILD++NAP+PEFP NEV VGVI+MEDVIEELLQEEIL
Sbjct: 373 SVPVFKKRHRGCSFCILDVENAPLPEFPPNEVAVGVITMEDVIEELLQEEIL 424


>emb|CBI19695.3| unnamed protein product [Vitis vinifera]
          Length = 524

 Score =  525 bits (1352), Expect = e-146
 Identities = 262/329 (79%), Positives = 288/329 (87%)
 Frame = -2

Query: 1291 MAADIPCCGTQFALYILVSIGLVXXXXXXXXXXXXXXXXXXXXLEVLIKSGRPQDRKHAA 1112
            MAAD+ CCGT+F +Y+++ +GLV                    LEVLIKSGRPQDR HAA
Sbjct: 1    MAADVACCGTKFFIYVVIIVGLVGFAGLMAGLTLGLMSLGLVDLEVLIKSGRPQDRIHAA 60

Query: 1111 KIFPVVKNQHLLLCTLLIGNSLAMESLPIFLDKLLPPWAAILLSVTLILMFAEILPQAIC 932
            KIFPVVKNQHLLLCTLLIGNSLAMESLPIFLDKL+PPWAAIL+SVTLILMF EILPQA+C
Sbjct: 61   KIFPVVKNQHLLLCTLLIGNSLAMESLPIFLDKLVPPWAAILISVTLILMFGEILPQALC 120

Query: 931  TRYGLTVGATVAPFVQLLLWLFFPIAYPISKVLDWMLGKGHAALLRRAELKTFVDFHGNE 752
            TRYG+TVGAT+APFV++LL LF+PIAYPISKVLDWMLGKGHAALLRRAELKTFVDFHGNE
Sbjct: 121  TRYGMTVGATMAPFVRVLLLLFYPIAYPISKVLDWMLGKGHAALLRRAELKTFVDFHGNE 180

Query: 751  AGKGGDLTHDETTIIAGALELTEKTVKDAMTPISKAFSIDLDGTLSLETLNAIMTMGHSR 572
            AGKGGDLTHDETTIIAGALELTEKT KDAMTPISKAFS+DLDGTL+LETLNAIMT+GHSR
Sbjct: 181  AGKGGDLTHDETTIIAGALELTEKTAKDAMTPISKAFSLDLDGTLTLETLNAIMTIGHSR 240

Query: 571  VPVYCGKPTNIVGIILVKNLLTVDPVDAVPLRKMLIRKVPRVSEDMPLYDILNEFQKGHS 392
            VPVY GKPTNI+G+ILVKNLL VDP DAVPLRKM+IRK+PRVSE+MPLYDILNEFQKGHS
Sbjct: 241  VPVYAGKPTNIIGLILVKNLLMVDPDDAVPLRKMVIRKIPRVSENMPLYDILNEFQKGHS 300

Query: 391  HIAVVYKDLNGTNEFSKKTNDATQNMASK 305
            HIAVV+KDLN T E   KT D    ++ K
Sbjct: 301  HIAVVFKDLNETKEAQNKTKDGALQVSMK 329



 Score = 93.6 bits (231), Expect = 1e-16
 Identities = 42/52 (80%), Positives = 48/52 (92%)
 Frame = -1

Query: 158 AAPAFKKRHKGCSFCILDLDNAPVPEFPSNEVVVGVISMEDVIEELLQEEIL 3
           + P FKKRH+GCSFCILD++NAP+PEFP NEV VGVI+MEDVIEELLQEEIL
Sbjct: 371 SVPVFKKRHRGCSFCILDVENAPLPEFPPNEVAVGVITMEDVIEELLQEEIL 422