BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00007504
         (627 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

pdb|2CJP|A Chain A, Structure Of Potato (Solanum Tuberosum) Epox...   270   1e-70
gb|AAA81889.1| epoxide hydrolase [Solanum tuberosum]                  270   2e-70
gb|AAA81892.1| epoxide hydrolase [Solanum tuberosum]                  269   3e-70
gb|AAA81891.1| epoxide hydrolase [Solanum tuberosum]                  269   4e-70
pdb|3CXU|A Chain A, Structure Of A Y149f Mutant Of Epoxide Hydro...   269   4e-70

>pdb|2CJP|A Chain A, Structure Of Potato (Solanum Tuberosum) Epoxide Hydrolase
           I (Steh1) gi|110590994|pdb|2CJP|B Chain B, Structure Of
           Potato (Solanum Tuberosum) Epoxide Hydrolase I (Steh1)
          Length = 328

 Score =  270 bits (691), Expect = 1e-70
 Identities = 126/203 (62%), Positives = 154/203 (75%)
 Frame = +2

Query: 5   SHN*KKMEEIKHTVISVNGLNMHVAEKGEGPIILFLHGFPELWYSWRHQINFMASHGYRA 184
           SH+   M++I+H +++VNGLNMH+AE GEGP ILF+HGFPELWYSWRHQ+ ++A  GYRA
Sbjct: 2   SHHHHHMKKIEHKMVAVNGLNMHLAELGEGPTILFIHGFPELWYSWRHQMVYLAERGYRA 61

Query: 185 VAPDLRGYGDTTGAPTDDVSKFTTXXXXXXXXXXXXAIAAEDEKVFVVGHDWGAFIAWHL 364
           VAPDLRGYGDTTGAP +D SKF+             AIA  +EKVFVV HDWGA IAWHL
Sbjct: 62  VAPDLRGYGDTTGAPLNDPSKFSILHLVGDVVALLEAIAPNEEKVFVVAHDWGALIAWHL 121

Query: 365 CLFRPDKVKALVNMSLEFIPWNPKGDYCEQMRGIYGDNHYMYRFQVPGEIESELEPIGFK 544
           CLFRPDKVKALVN+S+ F   NPK +  E ++ IYG++HY+ RFQVPGEIE+E  PIG K
Sbjct: 122 CLFRPDKVKALVNLSVHFSKRNPKMNVVEGLKAIYGEDHYISRFQVPGEIEAEFAPIGAK 181

Query: 545 NLMKIFLTSRNPGPLLFPKGHSL 613
           +++K  LT R+P P  FPKG  L
Sbjct: 182 SVLKKILTYRDPAPFYFPKGKGL 204


>gb|AAA81889.1| epoxide hydrolase [Solanum tuberosum]
          Length = 321

 Score =  270 bits (690), Expect = 2e-70
 Identities = 125/197 (63%), Positives = 152/197 (77%)
 Frame = +2

Query: 23  MEEIKHTVISVNGLNMHVAEKGEGPIILFLHGFPELWYSWRHQINFMASHGYRAVAPDLR 202
           ME+I+H +++VNGLNMH+AE G+GP ILFLHGFPELWYSWRHQ+ ++A  GYRAVAPDLR
Sbjct: 1   MEKIEHKMVAVNGLNMHIAELGQGPTILFLHGFPELWYSWRHQMVYLAECGYRAVAPDLR 60

Query: 203 GYGDTTGAPTDDVSKFTTXXXXXXXXXXXXAIAAEDEKVFVVGHDWGAFIAWHLCLFRPD 382
           GYGDTTGA  +D SKF+             AIA  +EKVFVV HDWGA IAWHLCLFRPD
Sbjct: 61  GYGDTTGASLNDPSKFSILHLVGDVVALLEAIAPNEEKVFVVAHDWGALIAWHLCLFRPD 120

Query: 383 KVKALVNMSLEFIPWNPKGDYCEQMRGIYGDNHYMYRFQVPGEIESELEPIGFKNLMKIF 562
           KVKALVN+S+ F+P NPK +  E ++ IYG++HY+ RFQVPGEIE+E  PIG K+++K  
Sbjct: 121 KVKALVNLSVHFLPRNPKMNTVEWLKAIYGEDHYISRFQVPGEIEAEFAPIGAKSILKKI 180

Query: 563 LTSRNPGPLLFPKGHSL 613
           LT R+P P  FPKG  L
Sbjct: 181 LTYRDPAPFYFPKGKGL 197


>gb|AAA81892.1| epoxide hydrolase [Solanum tuberosum]
          Length = 321

 Score =  269 bits (688), Expect = 3e-70
 Identities = 125/197 (63%), Positives = 151/197 (76%)
 Frame = +2

Query: 23  MEEIKHTVISVNGLNMHVAEKGEGPIILFLHGFPELWYSWRHQINFMASHGYRAVAPDLR 202
           ME+I+H +++VNGLNMH+AE GEGP ILF+HGFPELWYSWRHQ+ ++A  GYRAVAPDLR
Sbjct: 1   MEKIEHKMVAVNGLNMHLAELGEGPTILFIHGFPELWYSWRHQMVYLAERGYRAVAPDLR 60

Query: 203 GYGDTTGAPTDDVSKFTTXXXXXXXXXXXXAIAAEDEKVFVVGHDWGAFIAWHLCLFRPD 382
           GYGDTTGAP +D SKF+             AIA  +EKVFVV HDWGA IAWHLCLFRPD
Sbjct: 61  GYGDTTGAPLNDPSKFSILHLVGDVVALLEAIAPNEEKVFVVAHDWGALIAWHLCLFRPD 120

Query: 383 KVKALVNMSLEFIPWNPKGDYCEQMRGIYGDNHYMYRFQVPGEIESELEPIGFKNLMKIF 562
           KVKALVN+S+ F   NPK +  E ++ IYG++HY+ RFQVPGEIE+E  PIG K+++K  
Sbjct: 121 KVKALVNLSVHFSKRNPKMNVVEGLKAIYGEDHYISRFQVPGEIEAEFAPIGAKSVLKKI 180

Query: 563 LTSRNPGPLLFPKGHSL 613
           LT R+P P  FPKG  L
Sbjct: 181 LTYRDPAPFYFPKGKGL 197


>gb|AAA81891.1| epoxide hydrolase [Solanum tuberosum]
          Length = 321

 Score =  269 bits (687), Expect = 4e-70
 Identities = 124/197 (62%), Positives = 151/197 (76%)
 Frame = +2

Query: 23  MEEIKHTVISVNGLNMHVAEKGEGPIILFLHGFPELWYSWRHQINFMASHGYRAVAPDLR 202
           ME+I+H +++VNGLNMH+AE G+GP ILFLHGFPELWYSWRHQ+ ++A  GYRAVAP LR
Sbjct: 1   MEKIEHKMVAVNGLNMHIAELGQGPTILFLHGFPELWYSWRHQMVYLAERGYRAVAPVLR 60

Query: 203 GYGDTTGAPTDDVSKFTTXXXXXXXXXXXXAIAAEDEKVFVVGHDWGAFIAWHLCLFRPD 382
           GYGDTTGAP +D SKF+             AIA  +EKVFVV HDWGA IAWHLCLFRPD
Sbjct: 61  GYGDTTGAPLNDPSKFSILQLVGDVVALLEAIAPNEEKVFVVAHDWGALIAWHLCLFRPD 120

Query: 383 KVKALVNMSLEFIPWNPKGDYCEQMRGIYGDNHYMYRFQVPGEIESELEPIGFKNLMKIF 562
           KVKALVN S+ F+P NPK +  E ++ +YG++HY+ RFQVPGEIE+E  PIG K+++K  
Sbjct: 121 KVKALVNSSVHFLPRNPKMNTVEGLKAVYGEDHYISRFQVPGEIEAEFAPIGAKSVLKKI 180

Query: 563 LTSRNPGPLLFPKGHSL 613
           LT R+P P  FPKG  L
Sbjct: 181 LTFRDPAPFYFPKGKGL 197


>pdb|3CXU|A Chain A, Structure Of A Y149f Mutant Of Epoxide Hydrolase From
           Solanum Tuberosum gi|193885363|pdb|3CXU|B Chain B,
           Structure Of A Y149f Mutant Of Epoxide Hydrolase From
           Solanum Tuberosum
          Length = 328

 Score =  269 bits (687), Expect = 4e-70
 Identities = 125/203 (61%), Positives = 154/203 (75%)
 Frame = +2

Query: 5   SHN*KKMEEIKHTVISVNGLNMHVAEKGEGPIILFLHGFPELWYSWRHQINFMASHGYRA 184
           SH+   M++I+H +++VNGLNMH+AE GEGP ILF+HGFPELWYSWRHQ+ ++A  GYRA
Sbjct: 2   SHHHHHMKKIEHKMVAVNGLNMHLAELGEGPTILFIHGFPELWYSWRHQMVYLAERGYRA 61

Query: 185 VAPDLRGYGDTTGAPTDDVSKFTTXXXXXXXXXXXXAIAAEDEKVFVVGHDWGAFIAWHL 364
           VAPDLRGYGDTTGAP +D SKF+             AIA  +EKVFVV HDWGA IAWHL
Sbjct: 62  VAPDLRGYGDTTGAPLNDPSKFSILHLVGDVVALLEAIAPNEEKVFVVAHDWGALIAWHL 121

Query: 365 CLFRPDKVKALVNMSLEFIPWNPKGDYCEQMRGIYGDNHYMYRFQVPGEIESELEPIGFK 544
           CLFRPDKVKALVN+S+ F   NPK +  E ++ I+G++HY+ RFQVPGEIE+E  PIG K
Sbjct: 122 CLFRPDKVKALVNLSVHFSKRNPKMNVVEGLKAIFGEDHYISRFQVPGEIEAEFAPIGAK 181

Query: 545 NLMKIFLTSRNPGPLLFPKGHSL 613
           +++K  LT R+P P  FPKG  L
Sbjct: 182 SVLKKILTYRDPAPFYFPKGKGL 204