BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00007503
         (1545 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAA81889.1| epoxide hydrolase [Solanum tuberosum]                  342   1e-91
gb|AAA81891.1| epoxide hydrolase [Solanum tuberosum]                  342   1e-91
gb|AAA81892.1| epoxide hydrolase [Solanum tuberosum]                  338   2e-90
pdb|2CJP|A Chain A, Structure Of Potato (Solanum Tuberosum) Epox...   338   2e-90
pdb|3CXU|A Chain A, Structure Of A Y149f Mutant Of Epoxide Hydro...   337   5e-90

>gb|AAA81889.1| epoxide hydrolase [Solanum tuberosum]
          Length = 321

 Score =  342 bits (878), Expect = 1e-91
 Identities = 170/323 (52%), Positives = 214/323 (66%), Gaps = 1/323 (0%)
 Frame = +3

Query: 3   ELWYSWRHQINFMASHGYRAVAPDLRGYGDTTGAPTDDVSKFTTXXXXXXXXXXXXAIAG 182
           ELWYSWRHQ+ ++A  GYRAVAPDLRGYGDTTGA  +D SKF+             AIA 
Sbjct: 35  ELWYSWRHQMVYLAECGYRAVAPDLRGYGDTTGASLNDPSKFSILHLVGDVVALLEAIAP 94

Query: 183 EDEKVFVVGHDWGAFIAWHLCLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 362
            +EKVFVV HDWGA IAWHLCL                                      
Sbjct: 95  NEEKVFVVAHDWGALIAWHLCLFRPDKVKAL----------------------------- 125

Query: 363 XXXXVNMSLEFIPWNPKGDYCEQIRGIYGDNHYVYRFQVPGEIESVLEPIGFKNLMKIFL 542
               VN+S+ F+P NPK +  E ++ IYG++HY+ RFQVPGEIE+   PIG K+++K  L
Sbjct: 126 ----VNLSVHFLPRNPKMNTVEWLKAIYGEDHYISRFQVPGEIEAEFAPIGAKSILKKIL 181

Query: 543 TSRNPGPLLFPKGHSL-VNPDAPPPNIVLPSWISEEDIDYYASKFEKTGLTGGLNYYRAL 719
           T R+P P  FPKG  L   PDAP   + L SW+SEE++DYYA+KFE+TG TGG+NYYRAL
Sbjct: 182 TYRDPAPFYFPKGKGLEALPDAP---VALSSWLSEEELDYYANKFEQTGFTGGVNYYRAL 238

Query: 720 HLTWEHTAVWHKGKVMVPTKFVVGDLDLTYNVPGIKEYIHDGGMQKDVPLLQEVVVLEDT 899
            ++WE TA W   +V VPTKF+VG+ DL Y++PG KEYIH+GG ++DVPLL+EVVVLE +
Sbjct: 239 PISWELTAPWTGAQVKVPTKFIVGEFDLVYHIPGAKEYIHNGGFKEDVPLLEEVVVLEGS 298

Query: 900 AHFINQERANEVNKHILEFFQKF 968
           AHF+NQER +E++KHI +F QKF
Sbjct: 299 AHFVNQERPHEISKHIYDFIQKF 321


>gb|AAA81891.1| epoxide hydrolase [Solanum tuberosum]
          Length = 321

 Score =  342 bits (878), Expect = 1e-91
 Identities = 170/323 (52%), Positives = 211/323 (65%), Gaps = 1/323 (0%)
 Frame = +3

Query: 3   ELWYSWRHQINFMASHGYRAVAPDLRGYGDTTGAPTDDVSKFTTXXXXXXXXXXXXAIAG 182
           ELWYSWRHQ+ ++A  GYRAVAP LRGYGDTTGAP +D SKF+             AIA 
Sbjct: 35  ELWYSWRHQMVYLAERGYRAVAPVLRGYGDTTGAPLNDPSKFSILQLVGDVVALLEAIAP 94

Query: 183 EDEKVFVVGHDWGAFIAWHLCLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 362
            +EKVFVV HDWGA IAWHLCL                                      
Sbjct: 95  NEEKVFVVAHDWGALIAWHLCLFRPDKVKAL----------------------------- 125

Query: 363 XXXXVNMSLEFIPWNPKGDYCEQIRGIYGDNHYVYRFQVPGEIESVLEPIGFKNLMKIFL 542
               VN S+ F+P NPK +  E ++ +YG++HY+ RFQVPGEIE+   PIG K+++K  L
Sbjct: 126 ----VNSSVHFLPRNPKMNTVEGLKAVYGEDHYISRFQVPGEIEAEFAPIGAKSVLKKIL 181

Query: 543 TSRNPGPLLFPKGHSL-VNPDAPPPNIVLPSWISEEDIDYYASKFEKTGLTGGLNYYRAL 719
           T R+P P  FPKG  L   PDAP   + L SW+SEE++DYYA+KFE+TG TGG+NYYRAL
Sbjct: 182 TFRDPAPFYFPKGKGLEALPDAP---VALSSWLSEEELDYYANKFEQTGFTGGVNYYRAL 238

Query: 720 HLTWEHTAVWHKGKVMVPTKFVVGDLDLTYNVPGIKEYIHDGGMQKDVPLLQEVVVLEDT 899
            + WE TA W   +V VPTKF+VG+ DL Y++PG KEYIH+GG +KDVPLL+EVVVLE  
Sbjct: 239 SINWELTAPWTGAQVKVPTKFIVGEFDLVYHIPGAKEYIHNGGFKKDVPLLEEVVVLEGA 298

Query: 900 AHFINQERANEVNKHILEFFQKF 968
           AHF+NQER +E++KHI +F QKF
Sbjct: 299 AHFVNQERPHEISKHIYDFIQKF 321


>gb|AAA81892.1| epoxide hydrolase [Solanum tuberosum]
          Length = 321

 Score =  338 bits (868), Expect = 2e-90
 Identities = 169/323 (52%), Positives = 210/323 (65%), Gaps = 1/323 (0%)
 Frame = +3

Query: 3   ELWYSWRHQINFMASHGYRAVAPDLRGYGDTTGAPTDDVSKFTTXXXXXXXXXXXXAIAG 182
           ELWYSWRHQ+ ++A  GYRAVAPDLRGYGDTTGAP +D SKF+             AIA 
Sbjct: 35  ELWYSWRHQMVYLAERGYRAVAPDLRGYGDTTGAPLNDPSKFSILHLVGDVVALLEAIAP 94

Query: 183 EDEKVFVVGHDWGAFIAWHLCLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 362
            +EKVFVV HDWGA IAWHLCL                                      
Sbjct: 95  NEEKVFVVAHDWGALIAWHLCLFRPDKVKAL----------------------------- 125

Query: 363 XXXXVNMSLEFIPWNPKGDYCEQIRGIYGDNHYVYRFQVPGEIESVLEPIGFKNLMKIFL 542
               VN+S+ F   NPK +  E ++ IYG++HY+ RFQVPGEIE+   PIG K+++K  L
Sbjct: 126 ----VNLSVHFSKRNPKMNVVEGLKAIYGEDHYISRFQVPGEIEAEFAPIGAKSVLKKIL 181

Query: 543 TSRNPGPLLFPKGHSL-VNPDAPPPNIVLPSWISEEDIDYYASKFEKTGLTGGLNYYRAL 719
           T R+P P  FPKG  L   PDAP   + L SW+SEE++DYYA+KFE+TG TG +NYYRAL
Sbjct: 182 TYRDPAPFYFPKGKGLEAIPDAP---VALSSWLSEEELDYYANKFEQTGFTGAVNYYRAL 238

Query: 720 HLTWEHTAVWHKGKVMVPTKFVVGDLDLTYNVPGIKEYIHDGGMQKDVPLLQEVVVLEDT 899
            + WE TA W   +V VPTKF+VG+ DL Y++PG KEYIH+GG +KDVPLL+EVVVLE  
Sbjct: 239 PINWELTAPWTGAQVKVPTKFIVGEFDLVYHIPGAKEYIHNGGFKKDVPLLEEVVVLEGA 298

Query: 900 AHFINQERANEVNKHILEFFQKF 968
           AHF++QER +E++KHI +F QKF
Sbjct: 299 AHFVSQERPHEISKHIYDFIQKF 321


>pdb|2CJP|A Chain A, Structure Of Potato (Solanum Tuberosum) Epoxide Hydrolase
           I (Steh1) gi|110590994|pdb|2CJP|B Chain B, Structure Of
           Potato (Solanum Tuberosum) Epoxide Hydrolase I (Steh1)
          Length = 328

 Score =  338 bits (868), Expect = 2e-90
 Identities = 169/323 (52%), Positives = 210/323 (65%), Gaps = 1/323 (0%)
 Frame = +3

Query: 3   ELWYSWRHQINFMASHGYRAVAPDLRGYGDTTGAPTDDVSKFTTXXXXXXXXXXXXAIAG 182
           ELWYSWRHQ+ ++A  GYRAVAPDLRGYGDTTGAP +D SKF+             AIA 
Sbjct: 42  ELWYSWRHQMVYLAERGYRAVAPDLRGYGDTTGAPLNDPSKFSILHLVGDVVALLEAIAP 101

Query: 183 EDEKVFVVGHDWGAFIAWHLCLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 362
            +EKVFVV HDWGA IAWHLCL                                      
Sbjct: 102 NEEKVFVVAHDWGALIAWHLCLFRPDKVKAL----------------------------- 132

Query: 363 XXXXVNMSLEFIPWNPKGDYCEQIRGIYGDNHYVYRFQVPGEIESVLEPIGFKNLMKIFL 542
               VN+S+ F   NPK +  E ++ IYG++HY+ RFQVPGEIE+   PIG K+++K  L
Sbjct: 133 ----VNLSVHFSKRNPKMNVVEGLKAIYGEDHYISRFQVPGEIEAEFAPIGAKSVLKKIL 188

Query: 543 TSRNPGPLLFPKGHSL-VNPDAPPPNIVLPSWISEEDIDYYASKFEKTGLTGGLNYYRAL 719
           T R+P P  FPKG  L   PDAP   + L SW+SEE++DYYA+KFE+TG TG +NYYRAL
Sbjct: 189 TYRDPAPFYFPKGKGLEAIPDAP---VALSSWLSEEELDYYANKFEQTGFTGAVNYYRAL 245

Query: 720 HLTWEHTAVWHKGKVMVPTKFVVGDLDLTYNVPGIKEYIHDGGMQKDVPLLQEVVVLEDT 899
            + WE TA W   +V VPTKF+VG+ DL Y++PG KEYIH+GG +KDVPLL+EVVVLE  
Sbjct: 246 PINWELTAPWTGAQVKVPTKFIVGEFDLVYHIPGAKEYIHNGGFKKDVPLLEEVVVLEGA 305

Query: 900 AHFINQERANEVNKHILEFFQKF 968
           AHF++QER +E++KHI +F QKF
Sbjct: 306 AHFVSQERPHEISKHIYDFIQKF 328


>pdb|3CXU|A Chain A, Structure Of A Y149f Mutant Of Epoxide Hydrolase From
           Solanum Tuberosum gi|193885363|pdb|3CXU|B Chain B,
           Structure Of A Y149f Mutant Of Epoxide Hydrolase From
           Solanum Tuberosum
          Length = 328

 Score =  337 bits (864), Expect = 5e-90
 Identities = 168/323 (52%), Positives = 210/323 (65%), Gaps = 1/323 (0%)
 Frame = +3

Query: 3   ELWYSWRHQINFMASHGYRAVAPDLRGYGDTTGAPTDDVSKFTTXXXXXXXXXXXXAIAG 182
           ELWYSWRHQ+ ++A  GYRAVAPDLRGYGDTTGAP +D SKF+             AIA 
Sbjct: 42  ELWYSWRHQMVYLAERGYRAVAPDLRGYGDTTGAPLNDPSKFSILHLVGDVVALLEAIAP 101

Query: 183 EDEKVFVVGHDWGAFIAWHLCLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 362
            +EKVFVV HDWGA IAWHLCL                                      
Sbjct: 102 NEEKVFVVAHDWGALIAWHLCLFRPDKVKAL----------------------------- 132

Query: 363 XXXXVNMSLEFIPWNPKGDYCEQIRGIYGDNHYVYRFQVPGEIESVLEPIGFKNLMKIFL 542
               VN+S+ F   NPK +  E ++ I+G++HY+ RFQVPGEIE+   PIG K+++K  L
Sbjct: 133 ----VNLSVHFSKRNPKMNVVEGLKAIFGEDHYISRFQVPGEIEAEFAPIGAKSVLKKIL 188

Query: 543 TSRNPGPLLFPKGHSL-VNPDAPPPNIVLPSWISEEDIDYYASKFEKTGLTGGLNYYRAL 719
           T R+P P  FPKG  L   PDAP   + L SW+SEE++DYYA+KFE+TG TG +NYYRAL
Sbjct: 189 TYRDPAPFYFPKGKGLEAIPDAP---VALSSWLSEEELDYYANKFEQTGFTGAVNYYRAL 245

Query: 720 HLTWEHTAVWHKGKVMVPTKFVVGDLDLTYNVPGIKEYIHDGGMQKDVPLLQEVVVLEDT 899
            + WE TA W   +V VPTKF+VG+ DL Y++PG KEYIH+GG +KDVPLL+EVVVLE  
Sbjct: 246 PINWELTAPWTGAQVKVPTKFIVGEFDLVYHIPGAKEYIHNGGFKKDVPLLEEVVVLEGA 305

Query: 900 AHFINQERANEVNKHILEFFQKF 968
           AHF++QER +E++KHI +F QKF
Sbjct: 306 AHFVSQERPHEISKHIYDFIQKF 328