BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00007474
         (760 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|C0SJS2.1|C71AJ_PASSA RecName: Full=Psoralen synthase; AltName...   242   5e-62
emb|CBI14925.3| unnamed protein product [Vitis vinifera]              241   1e-61
sp|C0SJS3.1|ANGS_PASSA RecName: Full=Angelicin synthase; AltName...   240   2e-61
gb|AEI59775.1| cytochrome P450 [Helianthus annuus]                    239   4e-61
emb|CAN67678.1| hypothetical protein VITISV_035274 [Vitis vinifera]   239   4e-61

>sp|C0SJS2.1|C71AJ_PASSA RecName: Full=Psoralen synthase; AltName: Full=Cytochrome P450
           CYP71AJ3 gi|140083734|gb|ABO84853.1| cytochrome P450
           [Pastinaca sativa]
          Length = 473

 Score =  242 bits (618), Expect = 5e-62
 Identities = 134/253 (52%), Positives = 173/253 (68%), Gaps = 1/253 (0%)
 Frame = +3

Query: 3   IKDSGSDIVNLSELFVRLLSGIVSRIAVGKKYTXXXXXXXXXXXXFKELFEEIAQLIGLF 182
           I++S S ++NLSE+ + L   +V R A+G  Y             +K L  +I  ++G  
Sbjct: 152 IENSKSKVINLSEMLIELTGNVVCRAALGSGYNVDS---------YKSLLLQIMDMLGYS 202

Query: 183 -SVGDYIPWLYWVDILSGLKGRVEKAAKEVDAFLEVVIEDHISAMDNGVVSDDFLYILLE 359
            S+ D+ P L WVD ++GLKG+VEKAA  VDAFLE V+++H +   +   + DF+ ILLE
Sbjct: 203 RSIEDFFPSLGWVDWITGLKGKVEKAANGVDAFLEGVLKNHTNPSTSSA-NKDFVSILLE 261

Query: 360 NQKLNADTSAFSIDKESIKGVILNMFFDGTDTTAAVLEWTMAWLIKCPDIMSKLQSEVRE 539
            Q+ +A +S   +DKE IK +I +M   GT+T A  LEWT+  LIK PD MSKLQ EVRE
Sbjct: 262 IQEADAGSS---MDKECIKSLIWDMLGAGTETIATALEWTIGALIKSPDAMSKLQKEVRE 318

Query: 540 IGRGKSRISGDDIEKMHYLKAVIKESMRINIPVPLLVPREARQDVKVMGYDIRSGTQVII 719
           IG+GKSRI   D+ KM YLKAV+KESMR+    PLLVPREARQDVK MGYDI+SGTQV+I
Sbjct: 319 IGKGKSRIEEGDLVKMDYLKAVMKESMRLYFTAPLLVPREARQDVKFMGYDIKSGTQVLI 378

Query: 720 NAWAIARDPAVWD 758
           NAWAIARDP+ WD
Sbjct: 379 NAWAIARDPSSWD 391


>emb|CBI14925.3| unnamed protein product [Vitis vinifera]
          Length = 457

 Score =  241 bits (615), Expect = 1e-61
 Identities = 124/251 (49%), Positives = 172/251 (68%), Gaps = 4/251 (1%)
 Frame = +3

Query: 18  SDIVNLSELFVRLLSGIVSRIAVGKKYTXXXXXXXXXXXXFKELFEEIAQLIGLFSVGDY 197
           S  ++LS +F+RL + ++ R+A G+KY+            F+ L  E  +L+G F+VGDY
Sbjct: 122 STSMDLSGMFMRLTNDVICRVAFGRKYSGDERGKK-----FRRLLGEFVELLGGFNVGDY 176

Query: 198 IPWLYWVDILSGLKGRVEKAAKEVDAFLEVVIEDHIS----AMDNGVVSDDFLYILLENQ 365
           IPWL WV+ ++G   +VE+ AKE D FL+ V+E+H+     ++  G    DF+ +LLE Q
Sbjct: 177 IPWLAWVEYVNGWSAKVERVAKEFDEFLDGVVEEHLDGGTGSIAKGDNEKDFVDVLLEIQ 236

Query: 366 KLNADTSAFSIDKESIKGVILNMFFDGTDTTAAVLEWTMAWLIKCPDIMSKLQSEVREIG 545
           +    T  FS+D++SIK +IL++F  GTDTT  VLEW M  L++ P  M +LQ+EVR I 
Sbjct: 237 R--DGTLGFSMDRDSIKALILDIFAGGTDTTYTVLEWAMTELLRHPKAMKELQNEVRGIT 294

Query: 546 RGKSRISGDDIEKMHYLKAVIKESMRINIPVPLLVPREARQDVKVMGYDIRSGTQVIINA 725
           RGK  I+ DD+EKMHYLKAVIKE++R++ P+PLLVPRE+ QDV +MGY I +GT VIINA
Sbjct: 295 RGKEHITEDDLEKMHYLKAVIKETLRLHPPIPLLVPRESSQDVNIMGYHIPAGTMVIINA 354

Query: 726 WAIARDPAVWD 758
           WAI RDP  WD
Sbjct: 355 WAIGRDPMSWD 365


>sp|C0SJS3.1|ANGS_PASSA RecName: Full=Angelicin synthase; AltName: Full=Cytochrome P450
           CYP71AJ4 gi|140083745|gb|ABO84854.1| cytochrome P450
           [Pastinaca sativa]
          Length = 478

 Score =  240 bits (613), Expect = 2e-61
 Identities = 126/255 (49%), Positives = 178/255 (69%), Gaps = 3/255 (1%)
 Frame = +3

Query: 3   IKDSGSDIVNLSELFVRLLSGIVSRIAVGKKYTXXXXXXXXXXXXFKELFEEIAQLIGL- 179
           I++S S+++NL E  ++L   +V R++VG +Y             +++L  E+ +++   
Sbjct: 152 IENSASEVINLGEQLIQLTRNVVCRVSVGSEYLSGHKGKL-----YQKLLAEVTEMLAYT 206

Query: 180 FSVGDYIPWLYWVDILSGLKGRVEKAAKEVDAFLEVVIEDHISAM--DNGVVSDDFLYIL 353
           +S+GD+IP L WVD LSG K +VEK AKEVDAFLE  + DHI  M  + G  +DDFL IL
Sbjct: 207 YSIGDFIPLLGWVDWLSGSKAKVEKTAKEVDAFLEGALRDHIKTMASNKGSANDDFLSIL 266

Query: 354 LENQKLNADTSAFSIDKESIKGVILNMFFDGTDTTAAVLEWTMAWLIKCPDIMSKLQSEV 533
           LE ++ +A ++   +D+E IK ++ +M   GT+TT+  LEW +A +IK PD+M KLQ EV
Sbjct: 267 LEIREADAGST---LDEECIKAIVWDMILGGTETTSTTLEWIVAAIIKNPDVMFKLQKEV 323

Query: 534 REIGRGKSRISGDDIEKMHYLKAVIKESMRINIPVPLLVPREARQDVKVMGYDIRSGTQV 713
           REIG+GKS+I   D+ KM+YLKAV+KESMR+ I    L+PREA+QDVK+MGYDI SGTQV
Sbjct: 324 REIGKGKSKIEEVDLVKMNYLKAVMKESMRLYI-TAFLLPREAKQDVKLMGYDISSGTQV 382

Query: 714 IINAWAIARDPAVWD 758
           +IN W  ARDP++WD
Sbjct: 383 LINTWETARDPSLWD 397


>gb|AEI59775.1| cytochrome P450 [Helianthus annuus]
          Length = 491

 Score =  239 bits (611), Expect = 4e-61
 Identities = 119/252 (47%), Positives = 176/252 (69%)
 Frame = +3

Query: 3   IKDSGSDIVNLSELFVRLLSGIVSRIAVGKKYTXXXXXXXXXXXXFKELFEEIAQLIGLF 182
           I+ +   ++NLSEL   L + ++SR+A+G+KY              K + + I +L+  F
Sbjct: 162 IQQANESVINLSELINTLTNNVISRVALGRKYERNEV---------KAILDGIVELLASF 212

Query: 183 SVGDYIPWLYWVDILSGLKGRVEKAAKEVDAFLEVVIEDHISAMDNGVVSDDFLYILLEN 362
           SVG+YIPWL W+D L GL  R ++ AK+ D F+E V+++H +  ++ V   D + ILLE 
Sbjct: 213 SVGNYIPWLGWIDKLRGLHRRADELAKDQDDFVEGVLKEHENKKESHVERKDLVDILLEI 272

Query: 363 QKLNADTSAFSIDKESIKGVILNMFFDGTDTTAAVLEWTMAWLIKCPDIMSKLQSEVREI 542
           Q+ N+  + F +++  IK +I++MF  GTDTT+  LEWT+  L++ P +M KLQ E +++
Sbjct: 273 QRDNS--TGFQLERYMIKAIIMDMFGAGTDTTSTTLEWTLTELLRNPRVMKKLQQEAQKV 330

Query: 543 GRGKSRISGDDIEKMHYLKAVIKESMRINIPVPLLVPREARQDVKVMGYDIRSGTQVIIN 722
           G+G+S I   DI+KM YLKAV+KES+R++ PVPLLVPRE+ ++VK+MGYDI SGTQVIIN
Sbjct: 331 GQGRSFIPEGDIDKMPYLKAVLKESLRLHTPVPLLVPRESTKEVKIMGYDIPSGTQVIIN 390

Query: 723 AWAIARDPAVWD 758
           AWAIARDP++WD
Sbjct: 391 AWAIARDPSIWD 402


>emb|CAN67678.1| hypothetical protein VITISV_035274 [Vitis vinifera]
          Length = 505

 Score =  239 bits (611), Expect = 4e-61
 Identities = 123/251 (49%), Positives = 172/251 (68%), Gaps = 4/251 (1%)
 Frame = +3

Query: 18  SDIVNLSELFVRLLSGIVSRIAVGKKYTXXXXXXXXXXXXFKELFEEIAQLIGLFSVGDY 197
           S  ++LS +F+RL + ++ R+A G+KY+            F+ L  E  +L+G F+VGDY
Sbjct: 170 STSMDLSGMFMRLTNDVICRVAFGRKYSGDERGKK-----FRRLLGEFVELLGGFNVGDY 224

Query: 198 IPWLYWVDILSGLKGRVEKAAKEVDAFLEVVIEDHIS----AMDNGVVSDDFLYILLENQ 365
           IPWL WV+ ++G   +VE+ AKE D FL+ V+E+H+     ++  G    DF+ +LLE Q
Sbjct: 225 IPWLAWVEYVNGWXAKVERVAKEFDEFLDGVVEEHLDGGTGSIAKGDNEKDFVDVLLEIQ 284

Query: 366 KLNADTSAFSIDKESIKGVILNMFFDGTDTTAAVLEWTMAWLIKCPDIMSKLQSEVREIG 545
           +    T  FS+D++SIK +IL++F  GTDTT  VLEW M  L++ P  M +LQ+EVR I 
Sbjct: 285 R--DGTLGFSMDRDSIKALILDIFAGGTDTTYTVLEWAMTELLRHPKAMKELQNEVRGIT 342

Query: 546 RGKSRISGDDIEKMHYLKAVIKESMRINIPVPLLVPREARQDVKVMGYDIRSGTQVIINA 725
           RGK  I+ DD+EKMHYLKAVIKE++R++ P+PLLVPRE+ QDV +MGY I +GT VIINA
Sbjct: 343 RGKEHITEDDLEKMHYLKAVIKETLRLHPPIPLLVPRESSQDVNIMGYHIPAGTMVIINA 402

Query: 726 WAIARDPAVWD 758
           WA+ RDP  WD
Sbjct: 403 WAMGRDPMSWD 413