BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00007337
         (891 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI25377.3| unnamed protein product [Vitis vinifera]              444   e-122
ref|XP_002274607.1| PREDICTED: N(2),N(2)-dimethylguanosine tRNA ...   444   e-122
ref|XP_002319867.1| predicted protein [Populus trichocarpa] gi|2...   429   e-118
ref|XP_004158980.1| PREDICTED: tRNA (guanine(26)-N(2))-dimethylt...   422   e-116
ref|XP_004146475.1| PREDICTED: tRNA (guanine(26)-N(2))-dimethylt...   422   e-116

>emb|CBI25377.3| unnamed protein product [Vitis vinifera]
          Length = 338

 Score =  444 bits (1143), Expect = e-122
 Identities = 207/291 (71%), Positives = 244/291 (83%), Gaps = 1/291 (0%)
 Frame = +2

Query: 17  RRWTVTNDDANRVMMECCLRRDYYDFIDVDSFGSDSTFLRSAFGALRFGGLLYITSTDGY 196
           RRW VT+ DANRVM EC LRRD+YD IDVDSFGS+S+FLRSA+ AL+  GL Y+TSTDGY
Sbjct: 48  RRWVVTHTDANRVMTECYLRRDFYDLIDVDSFGSESSFLRSAYNALKLDGLFYVTSTDGY 107

Query: 197 TSGGHRPLHSLASYGAYVCPMPYCNELGLRMLIGGAVREASVLGYYVTPLFSYYSYHGPV 376
           TSGGHRP HSLASYGA+V PMPY NE+GLRMLIGGA+REASVLGY +TPLFSYYSYHGPV
Sbjct: 108 TSGGHRPHHSLASYGAFVRPMPYSNEVGLRMLIGGALREASVLGYRITPLFSYYSYHGPV 167

Query: 377 FRVLLRVDQGKIPISRHYNFIRYCNECGNSEAVAWDKLGQLSCPC-NTNVLGSLVVSGPL 553
           FRV+LR+++GK+P +R+Y FI YC +CGNS+A +W++LG++SCPC +  V  SLVVSGPL
Sbjct: 168 FRVMLRMNRGKLPENRNYGFISYCTKCGNSQAFSWNELGRISCPCSDAKVSSSLVVSGPL 227

Query: 554 WTGPLHSESYLKNMLTLAEAWGWIGNSNGVDLEKLLKHMMDEADPRLPFGYIKLDEVSSR 733
           W GPLH+ SYL  ML LAE WGW GN     L KLLK M+DE+DP+LPFGYIKLDEV+SR
Sbjct: 228 WIGPLHNASYLTEMLNLAEQWGWTGNDTETHLGKLLKQMIDESDPKLPFGYIKLDEVASR 287

Query: 734 GKVNQPPLRRMMSSIQKNGYAASRSHIESNALKTDCPMSECIMIAKELQAC 886
            K+N PPLR MMS++ K GYA SRSHI SNA+KT+CPM+ CI IAKELQ C
Sbjct: 288 AKINSPPLRTMMSALNKEGYAVSRSHIASNAIKTNCPMAVCIRIAKELQQC 338


>ref|XP_002274607.1| PREDICTED: N(2),N(2)-dimethylguanosine tRNA methyltransferase Trm1
            [Vitis vinifera] gi|147780177|emb|CAN75521.1|
            hypothetical protein VITISV_043594 [Vitis vinifera]
          Length = 424

 Score =  444 bits (1143), Expect = e-122
 Identities = 207/291 (71%), Positives = 244/291 (83%), Gaps = 1/291 (0%)
 Frame = +2

Query: 17   RRWTVTNDDANRVMMECCLRRDYYDFIDVDSFGSDSTFLRSAFGALRFGGLLYITSTDGY 196
            RRW VT+ DANRVM EC LRRD+YD IDVDSFGS+S+FLRSA+ AL+  GL Y+TSTDGY
Sbjct: 134  RRWVVTHTDANRVMTECYLRRDFYDLIDVDSFGSESSFLRSAYNALKLDGLFYVTSTDGY 193

Query: 197  TSGGHRPLHSLASYGAYVCPMPYCNELGLRMLIGGAVREASVLGYYVTPLFSYYSYHGPV 376
            TSGGHRP HSLASYGA+V PMPY NE+GLRMLIGGA+REASVLGY +TPLFSYYSYHGPV
Sbjct: 194  TSGGHRPHHSLASYGAFVRPMPYSNEVGLRMLIGGALREASVLGYRITPLFSYYSYHGPV 253

Query: 377  FRVLLRVDQGKIPISRHYNFIRYCNECGNSEAVAWDKLGQLSCPC-NTNVLGSLVVSGPL 553
            FRV+LR+++GK+P +R+Y FI YC +CGNS+A +W++LG++SCPC +  V  SLVVSGPL
Sbjct: 254  FRVMLRMNRGKLPENRNYGFISYCTKCGNSQAFSWNELGRISCPCSDAKVSSSLVVSGPL 313

Query: 554  WTGPLHSESYLKNMLTLAEAWGWIGNSNGVDLEKLLKHMMDEADPRLPFGYIKLDEVSSR 733
            W GPLH+ SYL  ML LAE WGW GN     L KLLK M+DE+DP+LPFGYIKLDEV+SR
Sbjct: 314  WIGPLHNASYLTEMLNLAEQWGWTGNDTETHLGKLLKQMIDESDPKLPFGYIKLDEVASR 373

Query: 734  GKVNQPPLRRMMSSIQKNGYAASRSHIESNALKTDCPMSECIMIAKELQAC 886
             K+N PPLR MMS++ K GYA SRSHI SNA+KT+CPM+ CI IAKELQ C
Sbjct: 374  AKINSPPLRTMMSALNKEGYAVSRSHIASNAIKTNCPMAVCIRIAKELQQC 424


>ref|XP_002319867.1| predicted protein [Populus trichocarpa] gi|222858243|gb|EEE95790.1|
            predicted protein [Populus trichocarpa]
          Length = 428

 Score =  429 bits (1102), Expect = e-118
 Identities = 202/290 (69%), Positives = 241/290 (83%), Gaps = 2/290 (0%)
 Frame = +2

Query: 17   RRWTVTNDDANRVMMECCLRRDYYDFIDVDSFGSD-STFLRSAFGALRFGGLLYITSTDG 193
            R+W VT+ DANRV+ EC L++D++D ID+DSFGSD S FLRSA   L F GLLY+TSTDG
Sbjct: 132  RKWVVTHLDANRVLSECYLQKDFFDLIDIDSFGSDISLFLRSAMNTLSFDGLLYVTSTDG 191

Query: 194  YTSGGHRPLHSLASYGAYVCPMPYCNELGLRMLIGGAVREASVLGYYVTPLFSYYSYHGP 373
            ++SGGH P HSLA+YGAYV PMPY NE+GLRMLIGGAVREASVLGYY+TPLFSYYSYHGP
Sbjct: 192  HSSGGHHPDHSLAAYGAYVRPMPYSNEVGLRMLIGGAVREASVLGYYITPLFSYYSYHGP 251

Query: 374  VFRVLLRVDQGKIPISRHYNFIRYCNECGNSEAVAWDKLGQLSCPCN-TNVLGSLVVSGP 550
            VFRV+LRV++GK+  +RHY FI YCN CGNS+  +W +LGQ+SC C+ ++   SLV+SGP
Sbjct: 252  VFRVMLRVNRGKLLENRHYGFITYCNNCGNSQEFSWAELGQISCSCSGSHGSRSLVISGP 311

Query: 551  LWTGPLHSESYLKNMLTLAEAWGWIGNSNGVDLEKLLKHMMDEADPRLPFGYIKLDEVSS 730
            LWTGPLHS +Y+  M+ LAE WGWIGN    DLEKLLK M+DE+DPRL  GYIK+DEV+S
Sbjct: 312  LWTGPLHSAAYISKMINLAEEWGWIGNGAETDLEKLLKQMLDESDPRLKSGYIKMDEVAS 371

Query: 731  RGKVNQPPLRRMMSSIQKNGYAASRSHIESNALKTDCPMSECIMIAKELQ 880
            R K+N PPLR MMS++QK GYA SRSHI SNA+KTDCPMS CI I+KELQ
Sbjct: 372  RAKINSPPLRTMMSAMQKEGYATSRSHIASNAIKTDCPMSVCIRISKELQ 421


>ref|XP_004158980.1| PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase-like [Cucumis
            sativus]
          Length = 440

 Score =  422 bits (1085), Expect = e-116
 Identities = 197/291 (67%), Positives = 240/291 (82%), Gaps = 1/291 (0%)
 Frame = +2

Query: 17   RRWTVTNDDANRVMMECCLRRDYYDFIDVDSFGSDSTFLRSAFGALRFGGLLYITSTDGY 196
            RRW VTN DANRVM E  L++D++DFIDVDSFGSDS+FLR A  AL+  GLLY+TSTDG+
Sbjct: 143  RRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGF 202

Query: 197  TSGGHRPLHSLASYGAYVCPMPYCNELGLRMLIGGAVREASVLGYYVTPLFSYYSYHGPV 376
            +SGGHRP  SLA+YGAYV PMP+ NE+GLRMLIGG  REASVLG+Y TPLFSYYSYHGPV
Sbjct: 203  SSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYATPLFSYYSYHGPV 262

Query: 377  FRVLLRVDQGKIPISRHYNFIRYCNECGNSEAVAWDKLGQLSCPCN-TNVLGSLVVSGPL 553
            FRV+LR+++G+I  +RHY F+ YC +CGNS+A +W +LGQ+SCPCN + V  SLVVSGPL
Sbjct: 263  FRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCNDSRVSESLVVSGPL 322

Query: 554  WTGPLHSESYLKNMLTLAEAWGWIGNSNGVDLEKLLKHMMDEADPRLPFGYIKLDEVSSR 733
            W GPLHS  Y++++LTLA+ W WIGN  G DLEKLL+ M+DE+D +LP GYIK+DEV+SR
Sbjct: 323  WIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQMVDESDSKLPVGYIKMDEVASR 382

Query: 734  GKVNQPPLRRMMSSIQKNGYAASRSHIESNALKTDCPMSECIMIAKELQAC 886
             KVN PPL  MMS + K GYAASRSHI SNA+KT+CPM+ECI IA+  + C
Sbjct: 383  AKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIAQLQRNC 433


>ref|XP_004146475.1| PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase-like [Cucumis
            sativus]
          Length = 467

 Score =  422 bits (1085), Expect = e-116
 Identities = 197/291 (67%), Positives = 240/291 (82%), Gaps = 1/291 (0%)
 Frame = +2

Query: 17   RRWTVTNDDANRVMMECCLRRDYYDFIDVDSFGSDSTFLRSAFGALRFGGLLYITSTDGY 196
            RRW VTN DANRVM E  L++D++DFIDVDSFGSDS+FLR A  AL+  GLLY+TSTDG+
Sbjct: 143  RRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGF 202

Query: 197  TSGGHRPLHSLASYGAYVCPMPYCNELGLRMLIGGAVREASVLGYYVTPLFSYYSYHGPV 376
            +SGGHRP  SLA+YGAYV PMP+ NE+GLRMLIGG  REASVLG+Y TPLFSYYSYHGPV
Sbjct: 203  SSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYATPLFSYYSYHGPV 262

Query: 377  FRVLLRVDQGKIPISRHYNFIRYCNECGNSEAVAWDKLGQLSCPCN-TNVLGSLVVSGPL 553
            FRV+LR+++G+I  +RHY F+ YC +CGNS+A +W +LGQ+SCPCN + V  SLVVSGPL
Sbjct: 263  FRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCNDSRVSESLVVSGPL 322

Query: 554  WTGPLHSESYLKNMLTLAEAWGWIGNSNGVDLEKLLKHMMDEADPRLPFGYIKLDEVSSR 733
            W GPLHS  Y++++LTLA+ W WIGN  G DLEKLL+ M+DE+D +LP GYIK+DEV+SR
Sbjct: 323  WIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQMVDESDSKLPVGYIKMDEVASR 382

Query: 734  GKVNQPPLRRMMSSIQKNGYAASRSHIESNALKTDCPMSECIMIAKELQAC 886
             KVN PPL  MMS + K GYAASRSHI SNA+KT+CPM+ECI IA+  + C
Sbjct: 383  AKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIAQLQRNC 433