BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00006670
         (655 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAT12274.1| obtusifoliol 14alpha-demethylase [Solanum chacoense]   393   e-107
ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum ly...   392   e-107
gb|ADN39418.1| obtusifoliol-14-demethylase [Withania somnifera]       392   e-107
gb|AAL40888.1| obtusifoliol-14-demethylase [Nicotiana tabacum]        387   e-106
gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana ...   386   e-105

>gb|AAT12274.1| obtusifoliol 14alpha-demethylase [Solanum chacoense]
          Length = 487

 Score =  393 bits (1009), Expect = e-107
 Identities = 187/217 (86%), Positives = 204/217 (94%)
 Frame = -3

Query: 653 ISVIFPYLPIPAHRRRDQARKKLAEIFGRIINSRKQAGKSENDMLQCFMDSKYKNGRPTT 474
           ISVIFPYLPIPAHRRRD ARKKLAEIF  IINSRK+ GK+ENDMLQCF+DSKYK+GRPTT
Sbjct: 212 ISVIFPYLPIPAHRRRDNARKKLAEIFANIINSRKRTGKAENDMLQCFIDSKYKDGRPTT 271

Query: 473 DSEVTGLLIAALFAGQHTSSITSTWTGAYLLANKKYLSAVSDEQKTIMKKHGNNVDHDIL 294
           + E+TGLLIAALFAGQHTSSITSTWTG+YLL N KY+SAV DEQK +MKKHGN VDHDIL
Sbjct: 272 EGEITGLLIAALFAGQHTSSITSTWTGSYLLTNNKYMSAVVDEQKNLMKKHGNKVDHDIL 331

Query: 293 SEMEALHRSIKEALRLHPPLIMLLRSSHSDFSVTTREGKEYDIPKGHIVATSPAFANRLP 114
           SEM+ L+R IKEALRLHPPLIMLLRSSHSDFSVTTREGKEYDIPKGHIVATSPAFANRLP
Sbjct: 332 SEMDVLYRCIKEALRLHPPLIMLLRSSHSDFSVTTREGKEYDIPKGHIVATSPAFANRLP 391

Query: 113 HIYKDPDTFDPDRFGPGRDEDKAAGPFSYISFGGGRH 3
           HI+K+P+++DPDRFGPGR+EDKAAG FSYISFGGGRH
Sbjct: 392 HIFKNPESYDPDRFGPGREEDKAAGAFSYISFGGGRH 428


>ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum lycopersicum]
           gi|299735181|gb|ADJ37071.1| obtusifoliol
           14alpha-demethylase [Solanum lycopersicum]
          Length = 487

 Score =  392 bits (1007), Expect = e-107
 Identities = 188/217 (86%), Positives = 203/217 (93%)
 Frame = -3

Query: 653 ISVIFPYLPIPAHRRRDQARKKLAEIFGRIINSRKQAGKSENDMLQCFMDSKYKNGRPTT 474
           ISVIFPYLPIPAHRRRD ARKKLAEIF  IINSRK+ GK+ENDMLQCF+DSKYK+GRPTT
Sbjct: 212 ISVIFPYLPIPAHRRRDNARKKLAEIFANIINSRKRTGKAENDMLQCFIDSKYKDGRPTT 271

Query: 473 DSEVTGLLIAALFAGQHTSSITSTWTGAYLLANKKYLSAVSDEQKTIMKKHGNNVDHDIL 294
           + E+TGLLIAALFAGQHTSSITSTWTG+YLL N KY+SAV DEQK +MKKHGN VDHDIL
Sbjct: 272 EGEITGLLIAALFAGQHTSSITSTWTGSYLLTNDKYMSAVVDEQKNLMKKHGNKVDHDIL 331

Query: 293 SEMEALHRSIKEALRLHPPLIMLLRSSHSDFSVTTREGKEYDIPKGHIVATSPAFANRLP 114
           SEME L+R IKEALRLHPPLIMLLRSSHS+FSVTTREGKEYDI KGHIVATSPAFANRLP
Sbjct: 332 SEMEVLYRCIKEALRLHPPLIMLLRSSHSEFSVTTREGKEYDISKGHIVATSPAFANRLP 391

Query: 113 HIYKDPDTFDPDRFGPGRDEDKAAGPFSYISFGGGRH 3
           HI+K+PDT+DPDRFGPGR+EDKAAG FSYISFGGGRH
Sbjct: 392 HIFKNPDTYDPDRFGPGREEDKAAGAFSYISFGGGRH 428


>gb|ADN39418.1| obtusifoliol-14-demethylase [Withania somnifera]
          Length = 487

 Score =  392 bits (1006), Expect = e-107
 Identities = 188/217 (86%), Positives = 202/217 (93%)
 Frame = -3

Query: 653 ISVIFPYLPIPAHRRRDQARKKLAEIFGRIINSRKQAGKSENDMLQCFMDSKYKNGRPTT 474
           ISVIFPYLPIPAHRRRD+ARKKLAEIF  IINSRK+ GK+ENDMLQCF+DSKYK+GR TT
Sbjct: 212 ISVIFPYLPIPAHRRRDKARKKLAEIFANIINSRKRTGKAENDMLQCFIDSKYKDGRATT 271

Query: 473 DSEVTGLLIAALFAGQHTSSITSTWTGAYLLANKKYLSAVSDEQKTIMKKHGNNVDHDIL 294
           + E+TGLLIAALFAGQHTSSITSTWTGAYLL NKKY+SAV DEQK +MKKHGN VDHDIL
Sbjct: 272 EGEITGLLIAALFAGQHTSSITSTWTGAYLLCNKKYMSAVVDEQKNLMKKHGNKVDHDIL 331

Query: 293 SEMEALHRSIKEALRLHPPLIMLLRSSHSDFSVTTREGKEYDIPKGHIVATSPAFANRLP 114
           SEME L+R IKEALRLHPPLIMLLR SHSDF VTT+EGKEYDIPKGHIVATSPAFANRLP
Sbjct: 332 SEMEVLYRCIKEALRLHPPLIMLLRHSHSDFCVTTKEGKEYDIPKGHIVATSPAFANRLP 391

Query: 113 HIYKDPDTFDPDRFGPGRDEDKAAGPFSYISFGGGRH 3
           HIYK+PDT+DPDRF PGR+EDKAAG FSYISFGGGRH
Sbjct: 392 HIYKNPDTYDPDRFSPGREEDKAAGAFSYISFGGGRH 428


>gb|AAL40888.1| obtusifoliol-14-demethylase [Nicotiana tabacum]
          Length = 487

 Score =  387 bits (995), Expect = e-106
 Identities = 186/217 (85%), Positives = 200/217 (92%)
 Frame = -3

Query: 653 ISVIFPYLPIPAHRRRDQARKKLAEIFGRIINSRKQAGKSENDMLQCFMDSKYKNGRPTT 474
           ISVIFPYLPIPAHRRRD ARKKLAEIF  II+SRK+ GK+E+DMLQCF+DSKYK+GR TT
Sbjct: 212 ISVIFPYLPIPAHRRRDNARKKLAEIFANIIDSRKRTGKAESDMLQCFIDSKYKDGRATT 271

Query: 473 DSEVTGLLIAALFAGQHTSSITSTWTGAYLLANKKYLSAVSDEQKTIMKKHGNNVDHDIL 294
           DSE+TGLLIAALFAGQHTSSITSTWTGAYLL N KY+SAV DEQK +MKKHGN VDHDIL
Sbjct: 272 DSEITGLLIAALFAGQHTSSITSTWTGAYLLCNNKYMSAVVDEQKNLMKKHGNKVDHDIL 331

Query: 293 SEMEALHRSIKEALRLHPPLIMLLRSSHSDFSVTTREGKEYDIPKGHIVATSPAFANRLP 114
           SEME L+R IKE LRLHPPLIMLLRSSHSDF+V TREGKEYDIPKGHIVATSPAFANRLP
Sbjct: 332 SEMEVLYRCIKEVLRLHPPLIMLLRSSHSDFTVKTREGKEYDIPKGHIVATSPAFANRLP 391

Query: 113 HIYKDPDTFDPDRFGPGRDEDKAAGPFSYISFGGGRH 3
           H+YK+PDT+DPDRF PGRDEDK AG FSYISFGGGRH
Sbjct: 392 HVYKNPDTYDPDRFTPGRDEDKVAGAFSYISFGGGRH 428


>gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana tabacum]
          Length = 487

 Score =  386 bits (992), Expect = e-105
 Identities = 185/217 (85%), Positives = 200/217 (92%)
 Frame = -3

Query: 653 ISVIFPYLPIPAHRRRDQARKKLAEIFGRIINSRKQAGKSENDMLQCFMDSKYKNGRPTT 474
           ISVIFPYLPIPAHRRRD ARKKLAEIF  II+SRK+ GK+E+DMLQCF+DSKYK+GR TT
Sbjct: 212 ISVIFPYLPIPAHRRRDNARKKLAEIFANIIDSRKRTGKAESDMLQCFIDSKYKDGRATT 271

Query: 473 DSEVTGLLIAALFAGQHTSSITSTWTGAYLLANKKYLSAVSDEQKTIMKKHGNNVDHDIL 294
           +SE+TGLLIAALFAGQHTSSITSTW GAYLL N KY+SAV DEQK +MKKHGN VDHDIL
Sbjct: 272 ESEITGLLIAALFAGQHTSSITSTWAGAYLLCNNKYMSAVVDEQKNLMKKHGNKVDHDIL 331

Query: 293 SEMEALHRSIKEALRLHPPLIMLLRSSHSDFSVTTREGKEYDIPKGHIVATSPAFANRLP 114
           SEME L+R IKEALRLHPPLIMLLRSSHSDF+V TREGKEYDIPKGHIVATSPAFANRLP
Sbjct: 332 SEMEVLYRCIKEALRLHPPLIMLLRSSHSDFTVKTREGKEYDIPKGHIVATSPAFANRLP 391

Query: 113 HIYKDPDTFDPDRFGPGRDEDKAAGPFSYISFGGGRH 3
           H+YK+PDT+DPDRF PGRDEDK AG FSYISFGGGRH
Sbjct: 392 HVYKNPDTYDPDRFAPGRDEDKVAGAFSYISFGGGRH 428