BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00005927
         (1195 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAS83469.1| cycloartenol synthase [Bupleurum kaoi]                 442   0.0  
sp|O82139.1|CAS1_PANGI RecName: Full=Cycloartenol Synthase gi|36...   414   e-179
gb|ABY60426.1| cycloartenol synthase [Panax notoginseng]              403   e-174
gb|AFC67276.1| cycloartenol synthase [Eleutherococcus senticosus]     403   e-172
gb|AAS01524.1| cycloartenol synthase [Centella asiatica]              399   e-171

>gb|AAS83469.1| cycloartenol synthase [Bupleurum kaoi]
          Length = 757

 Score =  442 bits (1138), Expect(3) = 0.0
 Identities = 199/201 (99%), Positives = 199/201 (99%)
 Frame = -3

Query: 1193 LGEGANDGQGAMEKGRQWILDRGGATNITSWGKMWLSVLGVFEWSGNNPLPPETWLVPYI 1014
            LGEGANDGQGAMEKGRQWILDRGGAT ITSWGKMWLSVLGVFEWSGNNPLPPETWLVPYI
Sbjct: 187  LGEGANDGQGAMEKGRQWILDRGGATKITSWGKMWLSVLGVFEWSGNNPLPPETWLVPYI 246

Query: 1013 LPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPIIISLRKELFTIPYHEIDWNQARNQCA 834
            LPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPIIISLRKELFTIPYHEIDWNQARNQCA
Sbjct: 247  LPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPIIISLRKELFTIPYHEIDWNQARNQCA 306

Query: 833  KEDLYYPHPLLQDILWASLDKVWEPIFMHWPAKKFREKSLCTVMEHIHYEDENTQYICIG 654
            KEDLYYPHPLLQDILW SLDKVWEPIFMHWPAKKFREKSLCTVMEHIHYEDENTQYICIG
Sbjct: 307  KEDLYYPHPLLQDILWDSLDKVWEPIFMHWPAKKFREKSLCTVMEHIHYEDENTQYICIG 366

Query: 653  PVNKVLNMLCCWVEDPNSEAF 591
            PVNKVLNMLCCWVEDPNSEAF
Sbjct: 367  PVNKVLNMLCCWVEDPNSEAF 387



 Score =  164 bits (415), Expect(3) = 0.0
 Identities = 74/75 (98%), Positives = 75/75 (100%)
 Frame = -2

Query: 501 WDTAFAVQAIISTNLIEQYGPTLKKAHLFIKNSQVLDNCPGDLKTWYRHISKGAWPFSTA 322
           W+TAFAVQAIISTNLIEQYGPTLKKAHLFIKNSQVLDNCPGDLKTWYRHISKGAWPFSTA
Sbjct: 416 WNTAFAVQAIISTNLIEQYGPTLKKAHLFIKNSQVLDNCPGDLKTWYRHISKGAWPFSTA 475

Query: 321 DHGWPISDCTAEGFK 277
           DHGWPISDCTAEGFK
Sbjct: 476 DHGWPISDCTAEGFK 490



 Score =  125 bits (315), Expect(3) = 0.0
 Identities = 60/60 (100%), Positives = 60/60 (100%)
 Frame = -1

Query: 181 PSEVVGEPLDTKRLHDAVNVILSLQNMDGSYATYELTRSYGWLESINPAETFGDIVIDYP 2
           PSEVVGEPLDTKRLHDAVNVILSLQNMDGSYATYELTRSYGWLESINPAETFGDIVIDYP
Sbjct: 499 PSEVVGEPLDTKRLHDAVNVILSLQNMDGSYATYELTRSYGWLESINPAETFGDIVIDYP 558


>sp|O82139.1|CAS1_PANGI RecName: Full=Cycloartenol Synthase gi|3688598|dbj|BAA33460.1|
            Cycloartenol Synthase [Panax ginseng]
          Length = 758

 Score =  414 bits (1063), Expect(3) = e-179
 Identities = 184/201 (91%), Positives = 192/201 (95%)
 Frame = -3

Query: 1193 LGEGANDGQGAMEKGRQWILDRGGATNITSWGKMWLSVLGVFEWSGNNPLPPETWLVPYI 1014
            LGEGANDGQGAMEKGRQWILD G AT ITSWGKMWLSVLGVFEWSGNNPLPPETWL+PYI
Sbjct: 187  LGEGANDGQGAMEKGRQWILDHGSATAITSWGKMWLSVLGVFEWSGNNPLPPETWLLPYI 246

Query: 1013 LPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPIIISLRKELFTIPYHEIDWNQARNQCA 834
            LP HPGRMWCH RMVYLPMSYLYGKRFVGPITP ++SLRKE+F++PYHEIDWNQARN CA
Sbjct: 247  LPIHPGRMWCHRRMVYLPMSYLYGKRFVGPITPTVLSLRKEVFSVPYHEIDWNQARNLCA 306

Query: 833  KEDLYYPHPLLQDILWASLDKVWEPIFMHWPAKKFREKSLCTVMEHIHYEDENTQYICIG 654
            KEDLYYPHPL+QDILWASLDKVWEPIFMHWPAKK REKSL TVMEHIHYEDENT+YICIG
Sbjct: 307  KEDLYYPHPLIQDILWASLDKVWEPIFMHWPAKKLREKSLRTVMEHIHYEDENTRYICIG 366

Query: 653  PVNKVLNMLCCWVEDPNSEAF 591
            PVNKVLNMLCCWVEDPNSEAF
Sbjct: 367  PVNKVLNMLCCWVEDPNSEAF 387



 Score =  152 bits (385), Expect(3) = e-179
 Identities = 66/77 (85%), Positives = 72/77 (93%)
 Frame = -2

Query: 501 WDTAFAVQAIISTNLIEQYGPTLKKAHLFIKNSQVLDNCPGDLKTWYRHISKGAWPFSTA 322
           WDTAFAVQAIISTNL E+YGPTL+KAH F+KNSQVLD+CPGDL  WYRH+SKGAWPFSTA
Sbjct: 416 WDTAFAVQAIISTNLAEEYGPTLRKAHTFMKNSQVLDDCPGDLDAWYRHVSKGAWPFSTA 475

Query: 321 DHGWPISDCTAEGFKVL 271
           DHGWPISDCTAEGFK +
Sbjct: 476 DHGWPISDCTAEGFKAV 492



 Score =  111 bits (278), Expect(3) = e-179
 Identities = 52/60 (86%), Positives = 55/60 (91%)
 Frame = -1

Query: 181 PSEVVGEPLDTKRLHDAVNVILSLQNMDGSYATYELTRSYGWLESINPAETFGDIVIDYP 2
           PSE+VGEPLD KRL+DAVNVILSLQN DG YATYELTRSY WLE +NPAETFGDIVIDYP
Sbjct: 499 PSELVGEPLDAKRLYDAVNVILSLQNSDGGYATYELTRSYSWLELVNPAETFGDIVIDYP 558


>gb|ABY60426.1| cycloartenol synthase [Panax notoginseng]
          Length = 758

 Score =  403 bits (1036), Expect(3) = e-174
 Identities = 180/201 (89%), Positives = 187/201 (93%)
 Frame = -3

Query: 1193 LGEGANDGQGAMEKGRQWILDRGGATNITSWGKMWLSVLGVFEWSGNNPLPPETWLVPYI 1014
            LGEG NDGQGAMEKGRQWILD GGAT I+SWGKMWLSVLGVFEWSGNNPLPPE WL PYI
Sbjct: 187  LGEGTNDGQGAMEKGRQWILDHGGATAISSWGKMWLSVLGVFEWSGNNPLPPEIWLFPYI 246

Query: 1013 LPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPIIISLRKELFTIPYHEIDWNQARNQCA 834
            LPFHPGRMWCHCRMVYLPMSYLYGKRFVGPIT  ++SLRKELFT+PYHEIDWNQARN CA
Sbjct: 247  LPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITSTVLSLRKELFTVPYHEIDWNQARNLCA 306

Query: 833  KEDLYYPHPLLQDILWASLDKVWEPIFMHWPAKKFREKSLCTVMEHIHYEDENTQYICIG 654
            KEDLYYPHPL+QDILWAS+DKV EPIFM WP KK REKSL TVMEHIHYEDENT+YICIG
Sbjct: 307  KEDLYYPHPLIQDILWASIDKVLEPIFMCWPGKKLREKSLRTVMEHIHYEDENTRYICIG 366

Query: 653  PVNKVLNMLCCWVEDPNSEAF 591
            PVNKVLNMLCCW EDPNSEAF
Sbjct: 367  PVNKVLNMLCCWAEDPNSEAF 387



 Score =  148 bits (374), Expect(3) = e-174
 Identities = 64/77 (83%), Positives = 71/77 (92%)
 Frame = -2

Query: 501 WDTAFAVQAIISTNLIEQYGPTLKKAHLFIKNSQVLDNCPGDLKTWYRHISKGAWPFSTA 322
           WDTAFAVQAIIST L +++GPTL+KAH+FIKNSQVLD+CPGDL  WYRHISKGAWPFST 
Sbjct: 416 WDTAFAVQAIISTKLTDEFGPTLRKAHMFIKNSQVLDDCPGDLNFWYRHISKGAWPFSTV 475

Query: 321 DHGWPISDCTAEGFKVL 271
           DHGWPISDCTAEGFK +
Sbjct: 476 DHGWPISDCTAEGFKAV 492



 Score =  109 bits (272), Expect(3) = e-174
 Identities = 52/59 (88%), Positives = 54/59 (91%)
 Frame = -1

Query: 178 SEVVGEPLDTKRLHDAVNVILSLQNMDGSYATYELTRSYGWLESINPAETFGDIVIDYP 2
           SE+VGEPLD KRL+DAVNVILSLQN DG YATYELTRSY WLE INPAETFGDIVIDYP
Sbjct: 500 SELVGEPLDAKRLYDAVNVILSLQNSDGGYATYELTRSYSWLELINPAETFGDIVIDYP 558


>gb|AFC67276.1| cycloartenol synthase [Eleutherococcus senticosus]
          Length = 758

 Score =  403 bits (1036), Expect(3) = e-172
 Identities = 180/201 (89%), Positives = 187/201 (93%)
 Frame = -3

Query: 1193 LGEGANDGQGAMEKGRQWILDRGGATNITSWGKMWLSVLGVFEWSGNNPLPPETWLVPYI 1014
            LGEGANDGQGAMEKGRQWILD GGA  ITSWGKMWLSVLGVFEWSGNNPLPPE WL+PYI
Sbjct: 187  LGEGANDGQGAMEKGRQWILDHGGAATITSWGKMWLSVLGVFEWSGNNPLPPEIWLLPYI 246

Query: 1013 LPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPIIISLRKELFTIPYHEIDWNQARNQCA 834
            LPF PGRMWCHCRMVYLPMSYLYGKRFVGPIT  ++SLRKELFT+PYHEIDWNQARN CA
Sbjct: 247  LPFPPGRMWCHCRMVYLPMSYLYGKRFVGPITSTVLSLRKELFTVPYHEIDWNQARNLCA 306

Query: 833  KEDLYYPHPLLQDILWASLDKVWEPIFMHWPAKKFREKSLCTVMEHIHYEDENTQYICIG 654
            KEDLYYPHPL+QDILWAS+DKV EPIFM WP KK REKSL TVMEHIHYEDENT+YICIG
Sbjct: 307  KEDLYYPHPLIQDILWASIDKVLEPIFMRWPGKKLREKSLRTVMEHIHYEDENTRYICIG 366

Query: 653  PVNKVLNMLCCWVEDPNSEAF 591
            PVNKVLNMLCCW EDPNSEAF
Sbjct: 367  PVNKVLNMLCCWAEDPNSEAF 387



 Score =  139 bits (351), Expect(3) = e-172
 Identities = 61/77 (79%), Positives = 69/77 (89%)
 Frame = -2

Query: 501 WDTAFAVQAIISTNLIEQYGPTLKKAHLFIKNSQVLDNCPGDLKTWYRHISKGAWPFSTA 322
           WDTAFAVQAIIST L +++GPTL+KAH+FIKNSQVLD+CPGD+  WYRHISKGA PFSTA
Sbjct: 416 WDTAFAVQAIISTKLTDEFGPTLRKAHMFIKNSQVLDDCPGDIDFWYRHISKGARPFSTA 475

Query: 321 DHGWPISDCTAEGFKVL 271
           DHGWP SDC AEGFK +
Sbjct: 476 DHGWPTSDCAAEGFKAV 492



 Score =  112 bits (279), Expect(3) = e-172
 Identities = 53/60 (88%), Positives = 55/60 (91%)
 Frame = -1

Query: 181 PSEVVGEPLDTKRLHDAVNVILSLQNMDGSYATYELTRSYGWLESINPAETFGDIVIDYP 2
           PSE+VGEPLD KRL+DAVNVILSLQN DG YATYELTRSY WLE INPAETFGDIVIDYP
Sbjct: 499 PSELVGEPLDAKRLYDAVNVILSLQNSDGGYATYELTRSYSWLELINPAETFGDIVIDYP 558


>gb|AAS01524.1| cycloartenol synthase [Centella asiatica]
          Length = 757

 Score =  399 bits (1025), Expect(3) = e-171
 Identities = 177/201 (88%), Positives = 185/201 (92%)
 Frame = -3

Query: 1193 LGEGANDGQGAMEKGRQWILDRGGATNITSWGKMWLSVLGVFEWSGNNPLPPETWLVPYI 1014
            LGEG  DGQGAMEKGRQWILD GGAT ITSWG+MWLSVLGVFEWSGNNPLPPE WL PY 
Sbjct: 187  LGEGTKDGQGAMEKGRQWILDHGGATAITSWGRMWLSVLGVFEWSGNNPLPPEIWLFPYN 246

Query: 1013 LPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPIIISLRKELFTIPYHEIDWNQARNQCA 834
            LPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITP ++SLRKELFT+PYHEIDWNQARN CA
Sbjct: 247  LPFHPGRMWCHCRMVYLPMSYLYGKRFVGPITPTVLSLRKELFTVPYHEIDWNQARNLCA 306

Query: 833  KEDLYYPHPLLQDILWASLDKVWEPIFMHWPAKKFREKSLCTVMEHIHYEDENTQYICIG 654
            KEDLYYPHPL+QDILWAS+DKV EPI M WP KK REK+L TVMEHIHYEDENT+YICIG
Sbjct: 307  KEDLYYPHPLVQDILWASIDKVLEPILMRWPGKKLREKALRTVMEHIHYEDENTRYICIG 366

Query: 653  PVNKVLNMLCCWVEDPNSEAF 591
            PVNKVLNMLCCW EDPNSEAF
Sbjct: 367  PVNKVLNMLCCWAEDPNSEAF 387



 Score =  144 bits (362), Expect(3) = e-171
 Identities = 64/77 (83%), Positives = 70/77 (90%)
 Frame = -2

Query: 501 WDTAFAVQAIISTNLIEQYGPTLKKAHLFIKNSQVLDNCPGDLKTWYRHISKGAWPFSTA 322
           WDTAFAVQAIIST+L E  GPTL+KAH F+K+SQVLD+CPGDL  WYRHISKGAWPFSTA
Sbjct: 416 WDTAFAVQAIISTSLTEYCGPTLRKAHTFMKDSQVLDDCPGDLDFWYRHISKGAWPFSTA 475

Query: 321 DHGWPISDCTAEGFKVL 271
           DHGWPISDCTAEGFK +
Sbjct: 476 DHGWPISDCTAEGFKAV 492



 Score =  110 bits (274), Expect(3) = e-171
 Identities = 52/60 (86%), Positives = 55/60 (91%)
 Frame = -1

Query: 181 PSEVVGEPLDTKRLHDAVNVILSLQNMDGSYATYELTRSYGWLESINPAETFGDIVIDYP 2
           P+E+VGEPLD KRL+DAVNVILSLQN DG YATYELTRSY WLE INPAETFGDIVIDYP
Sbjct: 499 PAELVGEPLDAKRLYDAVNVILSLQNSDGGYATYELTRSYRWLELINPAETFGDIVIDYP 558