BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00005888
         (1139 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAT39511.1| ferulate 5-hydroxylase [Camptotheca acuminata]         510   e-142
emb|CAN73481.1| hypothetical protein VITISV_026695 [Vitis vinifera]   505   e-141
ref|XP_002272644.1| PREDICTED: cytochrome P450 84A1 [Vitis vinif...   505   e-141
gb|ABC69406.1| CYP84A14v1 [Nicotiana tabacum]                         501   e-139
gb|AAS92625.1| coniferylalcohol 5-hydroxylase [Centaurium erythr...   500   e-139

>gb|AAT39511.1| ferulate 5-hydroxylase [Camptotheca acuminata]
          Length = 514

 Score =  510 bits (1314), Expect = e-142
 Identities = 255/347 (73%), Positives = 294/347 (84%)
 Frame = +1

Query: 1    LVFGLTRNIIYRAAFGTMSREGEDEFIKILQEFSKLFGAFNICDFVPGLTWADPQGFMGR 180
            LVF LTR+IIYRAAFG+ S EG+DEFIKILQEFSKLFGAFNI DF+PGL W DPQG   R
Sbjct: 177  LVFSLTRDIIYRAAFGSSSHEGQDEFIKILQEFSKLFGAFNIADFIPGLGWVDPQGLNAR 236

Query: 181  LVKARASLDGFIDTIIDEHIEKKKSSRNGFIDEGNSDMVYELLAFYSEDQHHQKPKVKDS 360
            LV+ARASLDGFID+IID+HIEK +  R   +D  + DMV +LLAFYS++      KV +S
Sbjct: 237  LVQARASLDGFIDSIIDDHIEKHR--RRNVVDS-DGDMVDDLLAFYSDEA-----KVTES 288

Query: 361  AEDINNSAVKLTRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKRVQQELNNVV 540
             +D+ N A++LTRDNIKAIIMDVMFGGTETVASAIEWAM+ELM+SPEDLKRVQQEL  VV
Sbjct: 289  -DDLQN-AIRLTRDNIKAIIMDVMFGGTETVASAIEWAMAELMKSPEDLKRVQQELAEVV 346

Query: 541  GLNRRVEESDFDKLTFLKCCIKETXXXXXXXXXXXXETAKDAEVGGYYVPAKARVIINSW 720
            GL+RR+EESDFDKLT+LKC +KET            ETA+D EV GYY+PA++RV+IN+W
Sbjct: 347  GLDRRLEESDFDKLTYLKCALKETLRLHPPIPLLLHETAEDTEVAGYYIPARSRVMINAW 406

Query: 721  AINRDPNSWEDPDMFKPSRFLKEGMPDFKGSNFEFLPFGSGRRSCPGMQLGLYALEIAVA 900
            AI RD NSW++P+ FKPSRFLKEG+PDFKGSNFEF+PFGSGRRSCPGMQLGLYALE+AVA
Sbjct: 407  AIGRDKNSWDEPETFKPSRFLKEGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALEMAVA 466

Query: 901  HLLHCFDWELPDGMKPSEVDTDDVFGLTAPRATRLVAVPTPRLLCPI 1041
            HLLHCF W LPDGMKPSE+DTDDVFGLTAPRAT  VAVP+PRLLCP+
Sbjct: 467  HLLHCFTWTLPDGMKPSELDTDDVFGLTAPRATLFVAVPSPRLLCPL 513


>emb|CAN73481.1| hypothetical protein VITISV_026695 [Vitis vinifera]
          Length = 493

 Score =  505 bits (1301), Expect = e-141
 Identities = 251/347 (72%), Positives = 293/347 (84%)
 Frame = +1

Query: 1    LVFGLTRNIIYRAAFGTMSREGEDEFIKILQEFSKLFGAFNICDFVPGLTWADPQGFMGR 180
            LVF LT+NIIYRAAFGT S+EG+DEFI ILQEFSKLFGAFNI DF+P L+W DPQG   R
Sbjct: 155  LVFTLTKNIIYRAAFGTSSKEGQDEFISILQEFSKLFGAFNIADFIPWLSWVDPQGLNAR 214

Query: 181  LVKARASLDGFIDTIIDEHIEKKKSSRNGFIDEGNSDMVYELLAFYSEDQHHQKPKVKDS 360
            L KAR SLDGFID IID+H++KKK + +   DE ++DMV +LLAFYSE+      KV +S
Sbjct: 215  LAKARKSLDGFIDDIIDDHMQKKKLNNDS--DEVDTDMVDDLLAFYSEEA-----KVNES 267

Query: 361  AEDINNSAVKLTRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKRVQQELNNVV 540
             ED+ N A++LTRDNIKAIIMDVMFGGTETVASAIEWAM+E+M+SPEDLK+VQQEL +VV
Sbjct: 268  -EDLQN-AIELTRDNIKAIIMDVMFGGTETVASAIEWAMAEMMKSPEDLKKVQQELADVV 325

Query: 541  GLNRRVEESDFDKLTFLKCCIKETXXXXXXXXXXXXETAKDAEVGGYYVPAKARVIINSW 720
            GLNRRVEESD +KLT+LKC +KET            ETA+DAEV GY++PA++RV+IN+W
Sbjct: 326  GLNRRVEESDLEKLTYLKCALKETLRLHPPIPLLLHETAEDAEVAGYHIPARSRVMINAW 385

Query: 721  AINRDPNSWEDPDMFKPSRFLKEGMPDFKGSNFEFLPFGSGRRSCPGMQLGLYALEIAVA 900
            AI RD NSW++P+ FKPSRFLK G+PDFKGSNFEF+PFGSGRRSCPGMQLGLYALE+AV 
Sbjct: 386  AIGRDKNSWDEPETFKPSRFLKAGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALELAVV 445

Query: 901  HLLHCFDWELPDGMKPSEVDTDDVFGLTAPRATRLVAVPTPRLLCPI 1041
            HLLHCF WELPDGMKPSE+D  DVFGLTAPRATRLVAVP+PRL CP+
Sbjct: 446  HLLHCFTWELPDGMKPSELDMGDVFGLTAPRATRLVAVPSPRLECPL 492


>ref|XP_002272644.1| PREDICTED: cytochrome P450 84A1 [Vitis vinifera]
          Length = 514

 Score =  505 bits (1300), Expect = e-141
 Identities = 251/347 (72%), Positives = 293/347 (84%)
 Frame = +1

Query: 1    LVFGLTRNIIYRAAFGTMSREGEDEFIKILQEFSKLFGAFNICDFVPGLTWADPQGFMGR 180
            LVF LT+NIIYRAAFGT S+EG+DEFI ILQEFSKLFGAFNI DF+P L+W DPQG   R
Sbjct: 176  LVFTLTKNIIYRAAFGTSSKEGQDEFISILQEFSKLFGAFNIADFIPWLSWVDPQGLNAR 235

Query: 181  LVKARASLDGFIDTIIDEHIEKKKSSRNGFIDEGNSDMVYELLAFYSEDQHHQKPKVKDS 360
            L KAR SLDGFID IID+H++KKK + +   DE ++DMV +LLAFYSE+      KV +S
Sbjct: 236  LAKARKSLDGFIDDIIDDHMQKKKLNNDS--DEVDTDMVDDLLAFYSEEA-----KVNES 288

Query: 361  AEDINNSAVKLTRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKRVQQELNNVV 540
             ED+ N A++LTRDNIKAIIMDVMFGGTETVASAIEWAM+E+M+SPEDLK+VQQEL +VV
Sbjct: 289  -EDLQN-AIELTRDNIKAIIMDVMFGGTETVASAIEWAMAEMMKSPEDLKKVQQELADVV 346

Query: 541  GLNRRVEESDFDKLTFLKCCIKETXXXXXXXXXXXXETAKDAEVGGYYVPAKARVIINSW 720
            GLNRRVEESD +KLT+LKC +KET            ETA+DAEV GY++PA++RV+IN+W
Sbjct: 347  GLNRRVEESDLEKLTYLKCVLKETLRLHPPIPLLLHETAEDAEVAGYHIPARSRVMINAW 406

Query: 721  AINRDPNSWEDPDMFKPSRFLKEGMPDFKGSNFEFLPFGSGRRSCPGMQLGLYALEIAVA 900
            AI RD NSW++P+ FKPSRFLK G+PDFKGSNFEF+PFGSGRRSCPGMQLGLYALE+AV 
Sbjct: 407  AIGRDKNSWDEPETFKPSRFLKAGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALELAVV 466

Query: 901  HLLHCFDWELPDGMKPSEVDTDDVFGLTAPRATRLVAVPTPRLLCPI 1041
            HLLHCF WELPDGMKPSE+D  DVFGLTAPRATRLVAVP+PRL CP+
Sbjct: 467  HLLHCFTWELPDGMKPSELDMGDVFGLTAPRATRLVAVPSPRLECPL 513


>gb|ABC69406.1| CYP84A14v1 [Nicotiana tabacum]
          Length = 525

 Score =  501 bits (1289), Expect = e-139
 Identities = 253/349 (72%), Positives = 291/349 (83%), Gaps = 2/349 (0%)
 Frame = +1

Query: 1    LVFGLTRNIIYRAAFGTMSREGEDEFIKILQEFSKLFGAFNICDFVPGLTWADPQGFMGR 180
            LVF LTRNIIYRAAFGT S +G+ EFIKI+QEFSKLFGAFNI DF+P L W   Q    R
Sbjct: 183  LVFSLTRNIIYRAAFGTCSEDGQGEFIKIMQEFSKLFGAFNIADFIPWLGWVGKQSLNIR 242

Query: 181  LVKARASLDGFIDTIIDEHIEKKKSSRNGFIDEGN--SDMVYELLAFYSEDQHHQKPKVK 354
            L KARASLDGFID+IID+HI +KK+  NG  D G+  +DMV ELLAFYSE+      KV 
Sbjct: 243  LAKARASLDGFIDSIIDDHIIRKKAYVNGKNDGGDRETDMVDELLAFYSEEA-----KVT 297

Query: 355  DSAEDINNSAVKLTRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKRVQQELNN 534
            +S ED+ N A++LT+DNIKAIIMDVMFGGTETVASAIEWAM+ELMRSPEDLK+VQQEL N
Sbjct: 298  ES-EDLQN-AIRLTKDNIKAIIMDVMFGGTETVASAIEWAMAELMRSPEDLKKVQQELAN 355

Query: 535  VVGLNRRVEESDFDKLTFLKCCIKETXXXXXXXXXXXXETAKDAEVGGYYVPAKARVIIN 714
            VVGLNR+VEESDF+KLT+L+CC+KET            ETA+++ V GY++PAK+ VIIN
Sbjct: 356  VVGLNRKVEESDFEKLTYLRCCLKETLRLHPPIPLLLHETAEESTVSGYHIPAKSHVIIN 415

Query: 715  SWAINRDPNSWEDPDMFKPSRFLKEGMPDFKGSNFEFLPFGSGRRSCPGMQLGLYALEIA 894
            S+AI RD NSWEDP+ +KPSRFLKEG+PDFKG NFEF+PFGSGRRSCPGMQLGLYALE+A
Sbjct: 416  SFAIGRDKNSWEDPETYKPSRFLKEGVPDFKGGNFEFIPFGSGRRSCPGMQLGLYALEMA 475

Query: 895  VAHLLHCFDWELPDGMKPSEVDTDDVFGLTAPRATRLVAVPTPRLLCPI 1041
            VAHLLHCF WELPDGMKPSE+  DD+FGLTAPRA RLVAVPTPRLLCP+
Sbjct: 476  VAHLLHCFTWELPDGMKPSELKMDDIFGLTAPRANRLVAVPTPRLLCPL 524


>gb|AAS92625.1| coniferylalcohol 5-hydroxylase [Centaurium erythraea]
          Length = 519

 Score =  500 bits (1288), Expect = e-139
 Identities = 246/347 (70%), Positives = 296/347 (85%)
 Frame = +1

Query: 1    LVFGLTRNIIYRAAFGTMSREGEDEFIKILQEFSKLFGAFNICDFVPGLTWADPQGFMGR 180
            +VFGLT+++IYRAAFGT S EG+DEFIKILQEFSKLFGAFN+ DF+P L+W DPQG   R
Sbjct: 180  MVFGLTKDVIYRAAFGTSSHEGQDEFIKILQEFSKLFGAFNLADFIPWLSWFDPQGLNKR 239

Query: 181  LVKARASLDGFIDTIIDEHIEKKKSSRNGFIDEGNSDMVYELLAFYSEDQHHQKPKVKDS 360
            L KARASLDGFID+IID+H+ +KK  ++G  D   SDMV ELL F+S++      KV +S
Sbjct: 240  LEKARASLDGFIDSIIDDHMNRKKI-KSGSNDVVESDMVDELLVFFSDEA-----KVLES 293

Query: 361  AEDINNSAVKLTRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKRVQQELNNVV 540
             ED+ NS +KLTRDNIKAIIMDVMFGGTETVASAIEWAM+ELMR+P+DLKRVQQEL++VV
Sbjct: 294  -EDLQNS-IKLTRDNIKAIIMDVMFGGTETVASAIEWAMAELMRNPDDLKRVQQELSDVV 351

Query: 541  GLNRRVEESDFDKLTFLKCCIKETXXXXXXXXXXXXETAKDAEVGGYYVPAKARVIINSW 720
            GL+R+V+ESDF+KLT+ KCC+KET            ETA+ +E+ GY +PAK+RV+IN+W
Sbjct: 352  GLHRKVQESDFEKLTYFKCCLKETLRLHPPIPLLLHETAEASEIAGYRIPAKSRVMINAW 411

Query: 721  AINRDPNSWEDPDMFKPSRFLKEGMPDFKGSNFEFLPFGSGRRSCPGMQLGLYALEIAVA 900
            AI RD +SW+DPD F+P+RFL+EG+PDFKGSNFEF+PFGSGRRSCPGMQLGLYALE+AVA
Sbjct: 412  AIGRDKDSWKDPDTFRPARFLEEGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALEVAVA 471

Query: 901  HLLHCFDWELPDGMKPSEVDTDDVFGLTAPRATRLVAVPTPRLLCPI 1041
            HLLHCF WELPDGMKPSE+D  DVFGLTAPRA+RLVAVP+PRLLCP+
Sbjct: 472  HLLHCFTWELPDGMKPSELDMGDVFGLTAPRASRLVAVPSPRLLCPL 518