BLASTX nr result
ID: Bupleurum21_contig00005859
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00005859
(1921 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|E0CSI1.2|MTBC1_VITVI RecName: Full=Probable bifunctional meth... 534 e-149
ref|XP_002285049.1| PREDICTED: probable bifunctional methylthior... 534 e-149
emb|CBI20280.3| unnamed protein product [Vitis vinifera] 528 e-147
ref|XP_002528256.1| catalytic, putative [Ricinus communis] gi|29... 527 e-147
ref|XP_002329082.1| predicted protein [Populus trichocarpa] gi|2... 522 e-145
>sp|E0CSI1.2|MTBC1_VITVI RecName: Full=Probable bifunctional methylthioribulose-1-phosphate
dehydratase/enolase-phosphatase E1 1; Includes: RecName:
Full=Methylthioribulose-1-phosphate dehydratase;
Short=MTRu-1-P dehydratase; Includes: RecName:
Full=Enolase-phosphatase E1; AltName:
Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase
Length = 517
Score = 534 bits (1376), Expect = e-149
Identities = 268/355 (75%), Positives = 298/355 (83%)
Frame = -2
Query: 1800 ASTAVNGVKLATATSSQAYLDSHQVKETKNLITELCRQFYRLGWVSGTGGSITIKVHDDC 1621
A+ A+NG+K+A +SQAYL+S V +T+ L+++LCR FY LGWVSGTGGSITIKVHD+
Sbjct: 2 AAAALNGLKMAA--TSQAYLESMPVNDTRKLLSDLCRHFYGLGWVSGTGGSITIKVHDES 59
Query: 1620 IPKPQQLILMSPSGVQKERMVEEDMYVLSPTGSVLFTXXXXXXXXXXXKCSDCAPLFMKA 1441
IPKPQQLI+MSPSGVQKERMV EDMYVLSP+G L + KCSDCAPLFMKA
Sbjct: 60 IPKPQQLIVMSPSGVQKERMVPEDMYVLSPSGCFLSSPSPKPYPNKPPKCSDCAPLFMKA 119
Query: 1440 YEMRNAGAVIHSHGMESCIATMINPLAKEFRITHMEMIKGIQGHGYYDELVVPIIENTAQ 1261
Y MRNAGAVIHSHGMESCI TMI+PL+KEFRITHMEMIKGIQGHGYYDELVVPIIENTA
Sbjct: 120 YLMRNAGAVIHSHGMESCIVTMIDPLSKEFRITHMEMIKGIQGHGYYDELVVPIIENTAH 179
Query: 1260 ERELTESLAAAIEAYPKSTAVLVRNHGVYIWGDSWISAKTQAECYHYLFEAAIKLHQLGL 1081
ERELT++LA AIEAYPK+TAVLVRNHG+YIWGDSWI AKTQAECYHYLF+AAIKL+QLGL
Sbjct: 180 ERELTDALAEAIEAYPKTTAVLVRNHGIYIWGDSWIHAKTQAECYHYLFDAAIKLYQLGL 239
Query: 1080 DCSTPNHGPIRSLKGLPNSDHNINMSVKAGIAVSNNEFEPSHRCIVLDIEGTTTPISFVT 901
D STP+HGPI+ G NMSVKAG SN+E EPS RCIVLDIEGTTTPISFVT
Sbjct: 240 DWSTPDHGPIKKYGG------KTNMSVKAGTVNSNHETEPSRRCIVLDIEGTTTPISFVT 293
Query: 900 DVLFPYARENVGNHLDATYETAETQDDIKLLRDQVNDDLKQGVEGALPIPPDDAG 736
DVLFP+AR NV HL ATYET ETQDDIKLLR QV DL+QG+ GA+PIPPDDAG
Sbjct: 294 DVLFPFARNNVSRHLAATYETDETQDDIKLLRTQVQSDLEQGIVGAVPIPPDDAG 348
Score = 247 bits (631), Expect = 7e-63
Identities = 114/137 (83%), Positives = 132/137 (96%)
Frame = -3
Query: 602 WRTGFQKSDIESVVFDDVPEALEKWHALGIKVYIYSSGSRLAQRLLFGYSNHGDLRKYLC 423
WRTGFQ +++E VVF+DVPEAL+KWHA GIKVYIYSSGSRLAQRLLFGY+N+GDLRKYLC
Sbjct: 381 WRTGFQNNELEGVVFEDVPEALQKWHASGIKVYIYSSGSRLAQRLLFGYTNYGDLRKYLC 440
Query: 422 GYFDTTVGNKKETRSYVEISEYLGVDKPSDILFVTDVYQEGVAAKAAGLDVIISVRPGNG 243
G+FDTTVGNK+ET+SYVEI+E +GVDKPS+ILFVTDVYQE VAAKAAGL+VIIS+RPGNG
Sbjct: 441 GFFDTTVGNKRETKSYVEITESVGVDKPSEILFVTDVYQEAVAAKAAGLEVIISIRPGNG 500
Query: 242 PLPENHGFKTVTSFSEI 192
PLP+NHGFKT+TSFS+I
Sbjct: 501 PLPDNHGFKTITSFSDI 517
>ref|XP_002285049.1| PREDICTED: probable bifunctional methylthioribulose-1-phosphate
dehydratase/enolase-phosphatase E1 1-like [Vitis
vinifera]
Length = 544
Score = 534 bits (1376), Expect = e-149
Identities = 268/355 (75%), Positives = 298/355 (83%)
Frame = -2
Query: 1800 ASTAVNGVKLATATSSQAYLDSHQVKETKNLITELCRQFYRLGWVSGTGGSITIKVHDDC 1621
A+ A+NG+K+A +SQAYL+S V +T+ L+++LCR FY LGWVSGTGGSITIKVHD+
Sbjct: 29 AAAALNGLKMAA--TSQAYLESMPVNDTRKLLSDLCRHFYGLGWVSGTGGSITIKVHDES 86
Query: 1620 IPKPQQLILMSPSGVQKERMVEEDMYVLSPTGSVLFTXXXXXXXXXXXKCSDCAPLFMKA 1441
IPKPQQLI+MSPSGVQKERMV EDMYVLSP+G L + KCSDCAPLFMKA
Sbjct: 87 IPKPQQLIVMSPSGVQKERMVPEDMYVLSPSGCFLSSPSPKPYPNKPPKCSDCAPLFMKA 146
Query: 1440 YEMRNAGAVIHSHGMESCIATMINPLAKEFRITHMEMIKGIQGHGYYDELVVPIIENTAQ 1261
Y MRNAGAVIHSHGMESCI TMI+PL+KEFRITHMEMIKGIQGHGYYDELVVPIIENTA
Sbjct: 147 YLMRNAGAVIHSHGMESCIVTMIDPLSKEFRITHMEMIKGIQGHGYYDELVVPIIENTAH 206
Query: 1260 ERELTESLAAAIEAYPKSTAVLVRNHGVYIWGDSWISAKTQAECYHYLFEAAIKLHQLGL 1081
ERELT++LA AIEAYPK+TAVLVRNHG+YIWGDSWI AKTQAECYHYLF+AAIKL+QLGL
Sbjct: 207 ERELTDALAEAIEAYPKTTAVLVRNHGIYIWGDSWIHAKTQAECYHYLFDAAIKLYQLGL 266
Query: 1080 DCSTPNHGPIRSLKGLPNSDHNINMSVKAGIAVSNNEFEPSHRCIVLDIEGTTTPISFVT 901
D STP+HGPI+ G NMSVKAG SN+E EPS RCIVLDIEGTTTPISFVT
Sbjct: 267 DWSTPDHGPIKKYGG------KTNMSVKAGTVNSNHETEPSRRCIVLDIEGTTTPISFVT 320
Query: 900 DVLFPYARENVGNHLDATYETAETQDDIKLLRDQVNDDLKQGVEGALPIPPDDAG 736
DVLFP+AR NV HL ATYET ETQDDIKLLR QV DL+QG+ GA+PIPPDDAG
Sbjct: 321 DVLFPFARNNVSRHLAATYETDETQDDIKLLRTQVQSDLEQGIVGAVPIPPDDAG 375
Score = 247 bits (631), Expect = 7e-63
Identities = 114/137 (83%), Positives = 132/137 (96%)
Frame = -3
Query: 602 WRTGFQKSDIESVVFDDVPEALEKWHALGIKVYIYSSGSRLAQRLLFGYSNHGDLRKYLC 423
WRTGFQ +++E VVF+DVPEAL+KWHA GIKVYIYSSGSRLAQRLLFGY+N+GDLRKYLC
Sbjct: 408 WRTGFQNNELEGVVFEDVPEALQKWHASGIKVYIYSSGSRLAQRLLFGYTNYGDLRKYLC 467
Query: 422 GYFDTTVGNKKETRSYVEISEYLGVDKPSDILFVTDVYQEGVAAKAAGLDVIISVRPGNG 243
G+FDTTVGNK+ET+SYVEI+E +GVDKPS+ILFVTDVYQE VAAKAAGL+VIIS+RPGNG
Sbjct: 468 GFFDTTVGNKRETKSYVEITESVGVDKPSEILFVTDVYQEAVAAKAAGLEVIISIRPGNG 527
Query: 242 PLPENHGFKTVTSFSEI 192
PLP+NHGFKT+TSFS+I
Sbjct: 528 PLPDNHGFKTITSFSDI 544
>emb|CBI20280.3| unnamed protein product [Vitis vinifera]
Length = 507
Score = 528 bits (1359), Expect = e-147
Identities = 263/343 (76%), Positives = 289/343 (84%)
Frame = -2
Query: 1764 ATSSQAYLDSHQVKETKNLITELCRQFYRLGWVSGTGGSITIKVHDDCIPKPQQLILMSP 1585
A +SQAYL+S V +T+ L+++LCR FY LGWVSGTGGSITIKVHD+ IPKPQQLI+MSP
Sbjct: 2 AATSQAYLESMPVNDTRKLLSDLCRHFYGLGWVSGTGGSITIKVHDESIPKPQQLIVMSP 61
Query: 1584 SGVQKERMVEEDMYVLSPTGSVLFTXXXXXXXXXXXKCSDCAPLFMKAYEMRNAGAVIHS 1405
SGVQKERMV EDMYVLSP+G L + KCSDCAPLFMKAY MRNAGAVIHS
Sbjct: 62 SGVQKERMVPEDMYVLSPSGCFLSSPSPKPYPNKPPKCSDCAPLFMKAYLMRNAGAVIHS 121
Query: 1404 HGMESCIATMINPLAKEFRITHMEMIKGIQGHGYYDELVVPIIENTAQERELTESLAAAI 1225
HGMESCI TMI+PL+KEFRITHMEMIKGIQGHGYYDELVVPIIENTA ERELT++LA AI
Sbjct: 122 HGMESCIVTMIDPLSKEFRITHMEMIKGIQGHGYYDELVVPIIENTAHERELTDALAEAI 181
Query: 1224 EAYPKSTAVLVRNHGVYIWGDSWISAKTQAECYHYLFEAAIKLHQLGLDCSTPNHGPIRS 1045
EAYPK+TAVLVRNHG+YIWGDSWI AKTQAECYHYLF+AAIKL+QLGLD STP+HGPI+
Sbjct: 182 EAYPKTTAVLVRNHGIYIWGDSWIHAKTQAECYHYLFDAAIKLYQLGLDWSTPDHGPIKK 241
Query: 1044 LKGLPNSDHNINMSVKAGIAVSNNEFEPSHRCIVLDIEGTTTPISFVTDVLFPYARENVG 865
G NMSVKAG SN+E EPS RCIVLDIEGTTTPISFVTDVLFP+AR NV
Sbjct: 242 YGG------KTNMSVKAGTVNSNHETEPSRRCIVLDIEGTTTPISFVTDVLFPFARNNVS 295
Query: 864 NHLDATYETAETQDDIKLLRDQVNDDLKQGVEGALPIPPDDAG 736
HL ATYET ETQDDIKLLR QV DL+QG+ GA+PIPPDDAG
Sbjct: 296 RHLAATYETDETQDDIKLLRTQVQSDLEQGIVGAVPIPPDDAG 338
Score = 247 bits (631), Expect = 7e-63
Identities = 114/137 (83%), Positives = 132/137 (96%)
Frame = -3
Query: 602 WRTGFQKSDIESVVFDDVPEALEKWHALGIKVYIYSSGSRLAQRLLFGYSNHGDLRKYLC 423
WRTGFQ +++E VVF+DVPEAL+KWHA GIKVYIYSSGSRLAQRLLFGY+N+GDLRKYLC
Sbjct: 371 WRTGFQNNELEGVVFEDVPEALQKWHASGIKVYIYSSGSRLAQRLLFGYTNYGDLRKYLC 430
Query: 422 GYFDTTVGNKKETRSYVEISEYLGVDKPSDILFVTDVYQEGVAAKAAGLDVIISVRPGNG 243
G+FDTTVGNK+ET+SYVEI+E +GVDKPS+ILFVTDVYQE VAAKAAGL+VIIS+RPGNG
Sbjct: 431 GFFDTTVGNKRETKSYVEITESVGVDKPSEILFVTDVYQEAVAAKAAGLEVIISIRPGNG 490
Query: 242 PLPENHGFKTVTSFSEI 192
PLP+NHGFKT+TSFS+I
Sbjct: 491 PLPDNHGFKTITSFSDI 507
>ref|XP_002528256.1| catalytic, putative [Ricinus communis]
gi|296439679|sp|B9SQI7.1|MTBC_RICCO RecName:
Full=Probable bifunctional methylthioribulose-1-phosphate
dehydratase/enolase-phosphatase E1; Includes: RecName:
Full=Methylthioribulose-1-phosphate dehydratase;
Short=MTRu-1-P dehydratase; Includes: RecName:
Full=Enolase-phosphatase E1; AltName:
Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase
gi|223532342|gb|EEF34141.1| catalytic, putative [Ricinus
communis]
Length = 527
Score = 527 bits (1357), Expect = e-147
Identities = 268/358 (74%), Positives = 298/358 (83%), Gaps = 1/358 (0%)
Frame = -2
Query: 1806 AAASTAVNG-VKLATATSSQAYLDSHQVKETKNLITELCRQFYRLGWVSGTGGSITIKVH 1630
AA + AVNG VK+A SQAYL+S VKET+ LI++LCRQFY LGWVSGTGGSITIKVH
Sbjct: 5 AAPAVAVNGGVKVA----SQAYLESKAVKETRVLISDLCRQFYNLGWVSGTGGSITIKVH 60
Query: 1629 DDCIPKPQQLILMSPSGVQKERMVEEDMYVLSPTGSVLFTXXXXXXXXXXXKCSDCAPLF 1450
DD IPKP QLILMSPSGVQKERM EDMYVL+ GS+L + KCSDC PLF
Sbjct: 61 DDSIPKPNQLILMSPSGVQKERMEPEDMYVLAANGSILSSPSPKPYPHKPPKCSDCGPLF 120
Query: 1449 MKAYEMRNAGAVIHSHGMESCIATMINPLAKEFRITHMEMIKGIQGHGYYDELVVPIIEN 1270
+KAYEM NAGAVIHSHGMESC+ TMINPL+KEF+ITHMEMIKGI+GHGYYDELVVPIIEN
Sbjct: 121 LKAYEMCNAGAVIHSHGMESCLVTMINPLSKEFKITHMEMIKGIKGHGYYDELVVPIIEN 180
Query: 1269 TAQERELTESLAAAIEAYPKSTAVLVRNHGVYIWGDSWISAKTQAECYHYLFEAAIKLHQ 1090
TA E ELT+SLA AIE YPK+TAVLVRNHG+YIWGDSWISAKTQAECYHYLF+AAIKLHQ
Sbjct: 181 TAYENELTDSLAKAIEEYPKTTAVLVRNHGIYIWGDSWISAKTQAECYHYLFDAAIKLHQ 240
Query: 1089 LGLDCSTPNHGPIRSLKGLPNSDHNINMSVKAGIAVSNNEFEPSHRCIVLDIEGTTTPIS 910
LGLD STPNHG I+++K L S+ +IN SVKAG+ SN+ + CIVLDIEGTTTPI+
Sbjct: 241 LGLDWSTPNHGLIQNVKALIGSNRDINTSVKAGLKDSNHGMQSLPGCIVLDIEGTTTPIT 300
Query: 909 FVTDVLFPYARENVGNHLDATYETAETQDDIKLLRDQVNDDLKQGVEGALPIPPDDAG 736
FV DVLFPYAR+NVG HL ATYETAETQDDIKLLR QV DDL +GV A+ IPPDDAG
Sbjct: 301 FVADVLFPYARDNVGRHLYATYETAETQDDIKLLRTQVEDDLARGVNEAVSIPPDDAG 358
Score = 230 bits (586), Expect = 1e-57
Identities = 107/137 (78%), Positives = 124/137 (90%)
Frame = -3
Query: 602 WRTGFQKSDIESVVFDDVPEALEKWHALGIKVYIYSSGSRLAQRLLFGYSNHGDLRKYLC 423
WRTGFQ +++E VV+DDVPEALEKWHALGIKVYIYSSGSRLAQRL+FG +N+GDLRKYL
Sbjct: 391 WRTGFQNNELEGVVYDDVPEALEKWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLS 450
Query: 422 GYFDTTVGNKKETRSYVEISEYLGVDKPSDILFVTDVYQEGVAAKAAGLDVIISVRPGNG 243
G+FDTTVGNK+ETRSY+EISE +GVD+PS+ILFVTDV QE VAAK AGL+ +IS+R GN
Sbjct: 451 GFFDTTVGNKRETRSYIEISESVGVDRPSEILFVTDVVQEAVAAKGAGLEAVISIRQGNA 510
Query: 242 PLPENHGFKTVTSFSEI 192
PLPENHGFKT+ S SEI
Sbjct: 511 PLPENHGFKTINSLSEI 527
>ref|XP_002329082.1| predicted protein [Populus trichocarpa]
gi|296439678|sp|B9N1F9.1|MTBC_POPTR RecName:
Full=Probable bifunctional methylthioribulose-1-phosphate
dehydratase/enolase-phosphatase E1; Includes: RecName:
Full=Methylthioribulose-1-phosphate dehydratase;
Short=MTRu-1-P dehydratase; Includes: RecName:
Full=Enolase-phosphatase E1; AltName:
Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase
gi|222869751|gb|EEF06882.1| predicted protein [Populus
trichocarpa]
Length = 504
Score = 522 bits (1345), Expect = e-145
Identities = 259/345 (75%), Positives = 286/345 (82%)
Frame = -2
Query: 1770 ATATSSQAYLDSHQVKETKNLITELCRQFYRLGWVSGTGGSITIKVHDDCIPKPQQLILM 1591
A +SQAYL+S VK+T+ LI +LC+QFY LGWVSGTGGSITIK HDD IPK QQLILM
Sbjct: 3 AAKVASQAYLESKAVKDTRVLIADLCKQFYTLGWVSGTGGSITIKAHDDSIPKRQQLILM 62
Query: 1590 SPSGVQKERMVEEDMYVLSPTGSVLFTXXXXXXXXXXXKCSDCAPLFMKAYEMRNAGAVI 1411
SPSGVQKERM EDMYVL+ GS+L + KCSDCAPLF+KAY+MRNAGAVI
Sbjct: 63 SPSGVQKERMEPEDMYVLATNGSILSSPSPKPYPYKPPKCSDCAPLFLKAYDMRNAGAVI 122
Query: 1410 HSHGMESCIATMINPLAKEFRITHMEMIKGIQGHGYYDELVVPIIENTAQERELTESLAA 1231
HSHGMESC+ TMINPL+KEFRITHMEMIKGIQGHGYYDELVVPIIENTA E ELT+SLA
Sbjct: 123 HSHGMESCLVTMINPLSKEFRITHMEMIKGIQGHGYYDELVVPIIENTAYENELTDSLAK 182
Query: 1230 AIEAYPKSTAVLVRNHGVYIWGDSWISAKTQAECYHYLFEAAIKLHQLGLDCSTPNHGPI 1051
AIEAYPK+TAVLVRNHG+YIWGDSWISAKTQAECYHYLF+AAIKLHQ+GLD STPNHGPI
Sbjct: 183 AIEAYPKTTAVLVRNHGIYIWGDSWISAKTQAECYHYLFDAAIKLHQIGLDWSTPNHGPI 242
Query: 1050 RSLKGLPNSDHNINMSVKAGIAVSNNEFEPSHRCIVLDIEGTTTPISFVTDVLFPYAREN 871
+ N+ VKAG+ SNN EP RCIVLDIEGTTTPI+FV DVLFPYAR+N
Sbjct: 243 Q------------NVKVKAGMNNSNNRIEPLPRCIVLDIEGTTTPITFVADVLFPYARDN 290
Query: 870 VGNHLDATYETAETQDDIKLLRDQVNDDLKQGVEGALPIPPDDAG 736
VG HL ATY+TAET+DDI LLR QV DDL QGV+GA+PIP DDAG
Sbjct: 291 VGRHLSATYDTAETKDDINLLRTQVEDDLAQGVDGAIPIPTDDAG 335
Score = 241 bits (614), Expect = 7e-61
Identities = 112/137 (81%), Positives = 128/137 (93%)
Frame = -3
Query: 602 WRTGFQKSDIESVVFDDVPEALEKWHALGIKVYIYSSGSRLAQRLLFGYSNHGDLRKYLC 423
WRTG++ +++E VV+DDVPEALEKWHALGIKVYIYSSGSRLAQRL+FG +N+GDLRKYL
Sbjct: 368 WRTGYENNELEGVVYDDVPEALEKWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLS 427
Query: 422 GYFDTTVGNKKETRSYVEISEYLGVDKPSDILFVTDVYQEGVAAKAAGLDVIISVRPGNG 243
G+FDTTVGNKKETRSY+EISE LGVDKPSDILFVTDV+QE AAK AGLDV+IS+RPGN
Sbjct: 428 GFFDTTVGNKKETRSYIEISESLGVDKPSDILFVTDVFQEAFAAKGAGLDVMISIRPGNA 487
Query: 242 PLPENHGFKTVTSFSEI 192
PLPENHGFKT+TSF+EI
Sbjct: 488 PLPENHGFKTITSFAEI 504