BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00005847
         (1698 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFK34139.1| unknown [Lotus japonicus]                              459   e-151
ref|XP_002264748.1| PREDICTED: 3-mercaptopyruvate sulfurtransfer...   455   e-150
gb|ABK94242.1| unknown [Populus trichocarpa]                          458   e-150
ref|XP_002310670.1| predicted protein [Populus trichocarpa] gi|2...   456   e-149
ref|XP_002513368.1| thiosulfate sulfertansferase, putative [Rici...   456   e-149

>gb|AFK34139.1| unknown [Lotus japonicus]
          Length = 384

 Score =  459 bits (1182), Expect(2) = e-151
 Identities = 227/324 (70%), Positives = 263/324 (81%), Gaps = 2/324 (0%)
 Frame = +3

Query: 495  MAATLMSRTLFATTRILQSSLTPHNTRFFSSSVFKMKPIHYWIAPSYTIR--KSFGYFXX 668
            MA+TL+S+ L     +  SS   H  R  SS +F  +       P+YT +  K+  +   
Sbjct: 3    MASTLLSKMLLGPRLVHSSSFLTHQPRVLSS-LFTKRVYCVRAVPAYTTQNSKTTRWASC 61

Query: 669  XXXXXXXXXXXXPVQSMSTSEPVVSVEWLHANLRDPDMKVLDASWYMPNEQRNPLQEYQV 848
                          QS+ST+EPVVSV+WL+ NL++PDMKVLDASWYMP+EQRNP+QEYQV
Sbjct: 62   MASSIVGRRATYSTQSVSTNEPVVSVDWLYDNLKEPDMKVLDASWYMPDEQRNPIQEYQV 121

Query: 849  AHIPGALFFDVDGISDQATNLPHMLPSEDAFAAAVSALGIENKDGVVVYDGKGIFSAARV 1028
            AHIPGALFFDVDGI+D+ TNLPHMLPSE+AFAAAVSALGI+NKD VVVYDGKG+FSAARV
Sbjct: 122  AHIPGALFFDVDGIADRTTNLPHMLPSEEAFAAAVSALGIQNKDDVVVYDGKGLFSAARV 181

Query: 1029 WWMFRVFGHDNVWVLDGGLPRWRASGFDVESSASSDAILKASAASDAIEKVYHGQTVAPL 1208
            WWMFRVFGHD VWVLDGGLPRWRASG+DVESSASSDAILKASAAS+AIEKVY GQ V P+
Sbjct: 182  WWMFRVFGHDRVWVLDGGLPRWRASGYDVESSASSDAILKASAASEAIEKVYQGQAVGPI 241

Query: 1209 TFQAKFQPHLVWNLEQVQRNIEDNSHQLIDARSKPRFDGAAPEPRKGIRSGHVPGSKCIP 1388
            TFQ KFQPHLVW L+QV++NIE+ +HQ +DARSKPRFDGAAPEPRKGIRSGHVPGSKCIP
Sbjct: 242  TFQTKFQPHLVWTLDQVKKNIEEKTHQQLDARSKPRFDGAAPEPRKGIRSGHVPGSKCIP 301

Query: 1389 FPQMLDGSQSLLPAESLKEKFEQE 1460
            F Q+LDGSQ+LLPA  LK++FEQE
Sbjct: 302  FSQLLDGSQTLLPAAELKKRFEQE 325



 Score =  103 bits (257), Expect(2) = e-151
 Identities = 46/52 (88%), Positives = 51/52 (98%)
 Frame = +1

Query: 1540 KEKFEQEGISLDRPIVTSCGTGVTACILALGLHRLGKTDVAVYDGSWTEWGA 1695
            K++FEQEGISL+ P+VTSCGTGVTACILALGLHRLGK+DVAVYDGSWTEWGA
Sbjct: 319  KKRFEQEGISLESPVVTSCGTGVTACILALGLHRLGKSDVAVYDGSWTEWGA 370


>ref|XP_002264748.1| PREDICTED: 3-mercaptopyruvate sulfurtransferase [Vitis vinifera]
            gi|147820684|emb|CAN69823.1| hypothetical protein
            VITISV_030676 [Vitis vinifera]
            gi|297736153|emb|CBI24191.3| unnamed protein product
            [Vitis vinifera]
          Length = 380

 Score =  455 bits (1170), Expect(2) = e-150
 Identities = 227/323 (70%), Positives = 258/323 (79%), Gaps = 1/323 (0%)
 Frame = +3

Query: 495  MAATLMSRTLFATTRILQSSLTPHNTRFFSSSVFKMKPIHYWIAPSYTIRKSFGYFXXXX 674
            MAA L +RTLF   R+++SSL  +      +S F  +P +      Y + +S G+     
Sbjct: 1    MAAALFARTLFGN-RLIRSSLALYQKPQIFTSFFNKRPFYSRPNAVYLVYRSSGFSTCAM 59

Query: 675  XXXXXXXXXX-PVQSMSTSEPVVSVEWLHANLRDPDMKVLDASWYMPNEQRNPLQEYQVA 851
                         QS+S +EPVVSV+WLHAN+R+PD+KVLDASWYMP+EQRNPLQEYQVA
Sbjct: 60   ASSTSGTRSSFSTQSVSPNEPVVSVDWLHANIREPDLKVLDASWYMPDEQRNPLQEYQVA 119

Query: 852  HIPGALFFDVDGISDQATNLPHMLPSEDAFAAAVSALGIENKDGVVVYDGKGIFSAARVW 1031
            HIPGALFFDVDGISDQ TNLPHMLPSE+AFAAAVSALGIENKDGVVVYDGKGIFSAARVW
Sbjct: 120  HIPGALFFDVDGISDQTTNLPHMLPSEEAFAAAVSALGIENKDGVVVYDGKGIFSAARVW 179

Query: 1032 WMFRVFGHDNVWVLDGGLPRWRASGFDVESSASSDAILKASAASDAIEKVYHGQTVAPLT 1211
            WMFRVFGH+ +WVLDGGLP WRASGFDVESSAS DAILKASAAS+AIEKVY  Q V P+T
Sbjct: 180  WMFRVFGHERIWVLDGGLPTWRASGFDVESSASGDAILKASAASEAIEKVYRRQAVGPIT 239

Query: 1212 FQAKFQPHLVWNLEQVQRNIEDNSHQLIDARSKPRFDGAAPEPRKGIRSGHVPGSKCIPF 1391
            FQ +FQPHLVW LEQV +NIE+ +HQ IDARSK RFDG APEPRKGIRSGHVP SKCIPF
Sbjct: 240  FQTRFQPHLVWTLEQVNQNIENKTHQHIDARSKARFDGIAPEPRKGIRSGHVPSSKCIPF 299

Query: 1392 PQMLDGSQSLLPAESLKEKFEQE 1460
             QMLD SQ LLPA+ LK++F+QE
Sbjct: 300  SQMLDSSQKLLPADELKKRFDQE 322



 Score =  105 bits (262), Expect(2) = e-150
 Identities = 46/53 (86%), Positives = 51/53 (96%)
 Frame = +1

Query: 1540 KEKFEQEGISLDRPIVTSCGTGVTACILALGLHRLGKTDVAVYDGSWTEWGAH 1698
            K++F+QEGISLD P+VTSCGTGVTACILALGLHRLGK+DV VYDGSWTEWGAH
Sbjct: 316  KKRFDQEGISLDSPVVTSCGTGVTACILALGLHRLGKSDVTVYDGSWTEWGAH 368


>gb|ABK94242.1| unknown [Populus trichocarpa]
          Length = 372

 Score =  458 bits (1178), Expect(2) = e-150
 Identities = 228/329 (69%), Positives = 262/329 (79%), Gaps = 7/329 (2%)
 Frame = +3

Query: 495  MAATLMSRTLFATTRILQSSLTPHNTRFFSSSVF-------KMKPIHYWIAPSYTIRKSF 653
            MA+T+++++L     I  S    H T    +S+F       +  PIH    PS  +R   
Sbjct: 1    MASTILTKSLLGHRLIHSSFSLSHKTPQIFTSLFPGRTFQKRADPIHTSYKPSQWVRCVV 60

Query: 654  GYFXXXXXXXXXXXXXXPVQSMSTSEPVVSVEWLHANLRDPDMKVLDASWYMPNEQRNPL 833
            G                  QS+S +EPVVSV+WLHANLR+PDMKVLDASWYMP+EQRNP+
Sbjct: 61   G-----------TRANFSTQSLSLNEPVVSVDWLHANLREPDMKVLDASWYMPDEQRNPI 109

Query: 834  QEYQVAHIPGALFFDVDGISDQATNLPHMLPSEDAFAAAVSALGIENKDGVVVYDGKGIF 1013
            QEYQVAHIPGALFFDVDGI+DQ TNLPHMLPSE+AFAAAVSALGIENKDG+VVYDGKGIF
Sbjct: 110  QEYQVAHIPGALFFDVDGIADQTTNLPHMLPSEEAFAAAVSALGIENKDGLVVYDGKGIF 169

Query: 1014 SAARVWWMFRVFGHDNVWVLDGGLPRWRASGFDVESSASSDAILKASAASDAIEKVYHGQ 1193
            SAARVWWMFRVFGH+ VWVLDGGLPRWRASG+DVESSAS DAILKASAAS+AIEKVY GQ
Sbjct: 170  SAARVWWMFRVFGHEKVWVLDGGLPRWRASGYDVESSASGDAILKASAASEAIEKVYQGQ 229

Query: 1194 TVAPLTFQAKFQPHLVWNLEQVQRNIEDNSHQLIDARSKPRFDGAAPEPRKGIRSGHVPG 1373
            TV P+T+Q KFQPHLVW LEQV+ NIED +HQ +DARSK RFDGAAPEPRKGI+SGHVPG
Sbjct: 230  TVGPITYQTKFQPHLVWTLEQVKSNIEDRTHQHVDARSKARFDGAAPEPRKGIKSGHVPG 289

Query: 1374 SKCIPFPQMLDGSQSLLPAESLKEKFEQE 1460
            SKCIPF Q+LD SQ+LLPA+ LK++F+QE
Sbjct: 290  SKCIPFAQLLDSSQTLLPADELKKRFDQE 318



 Score =  101 bits (252), Expect(2) = e-150
 Identities = 45/53 (84%), Positives = 51/53 (96%)
 Frame = +1

Query: 1540 KEKFEQEGISLDRPIVTSCGTGVTACILALGLHRLGKTDVAVYDGSWTEWGAH 1698
            K++F+QEGISL  P+VTSCGTGVTACILALGLHRLGK+DVAVYDGSWTEWGA+
Sbjct: 312  KKRFDQEGISLKSPVVTSCGTGVTACILALGLHRLGKSDVAVYDGSWTEWGAN 364


>ref|XP_002310670.1| predicted protein [Populus trichocarpa] gi|222853573|gb|EEE91120.1|
            predicted protein [Populus trichocarpa]
          Length = 372

 Score =  456 bits (1172), Expect(2) = e-149
 Identities = 227/329 (68%), Positives = 261/329 (79%), Gaps = 7/329 (2%)
 Frame = +3

Query: 495  MAATLMSRTLFATTRILQSSLTPHNTRFFSSSVF-------KMKPIHYWIAPSYTIRKSF 653
            MA+T+++++L     I  S    H T    +S+F       +  PIH    PS  +R   
Sbjct: 1    MASTILTKSLLGHRLIHSSFSLSHKTPQIFTSLFPGRTFQKRADPIHTSYKPSQWVRCVV 60

Query: 654  GYFXXXXXXXXXXXXXXPVQSMSTSEPVVSVEWLHANLRDPDMKVLDASWYMPNEQRNPL 833
            G                  QS+S +EPVV V+WLHANLR+PDMKVLDASWYMP+EQRNP+
Sbjct: 61   G-----------TRANFSTQSLSLNEPVVFVDWLHANLREPDMKVLDASWYMPDEQRNPI 109

Query: 834  QEYQVAHIPGALFFDVDGISDQATNLPHMLPSEDAFAAAVSALGIENKDGVVVYDGKGIF 1013
            QEYQVAHIPGALFFDVDGI+DQ TNLPHMLPSE+AFAAAVSALGIENKDG+VVYDGKGIF
Sbjct: 110  QEYQVAHIPGALFFDVDGIADQTTNLPHMLPSEEAFAAAVSALGIENKDGLVVYDGKGIF 169

Query: 1014 SAARVWWMFRVFGHDNVWVLDGGLPRWRASGFDVESSASSDAILKASAASDAIEKVYHGQ 1193
            SAARVWWMFRVFGH+ VWVLDGGLPRWRASG+DVESSAS DAILKASAAS+AIEKVY GQ
Sbjct: 170  SAARVWWMFRVFGHEKVWVLDGGLPRWRASGYDVESSASGDAILKASAASEAIEKVYQGQ 229

Query: 1194 TVAPLTFQAKFQPHLVWNLEQVQRNIEDNSHQLIDARSKPRFDGAAPEPRKGIRSGHVPG 1373
            TV P+T+Q KFQPHLVW LEQV+ NIED +HQ +DARSK RFDGAAPEPRKGI+SGHVPG
Sbjct: 230  TVGPITYQTKFQPHLVWTLEQVKSNIEDRTHQHVDARSKARFDGAAPEPRKGIKSGHVPG 289

Query: 1374 SKCIPFPQMLDGSQSLLPAESLKEKFEQE 1460
            SKCIPF Q+LD SQ+LLPA+ LK++F+QE
Sbjct: 290  SKCIPFAQLLDSSQTLLPADELKKRFDQE 318



 Score =  101 bits (252), Expect(2) = e-149
 Identities = 45/53 (84%), Positives = 51/53 (96%)
 Frame = +1

Query: 1540 KEKFEQEGISLDRPIVTSCGTGVTACILALGLHRLGKTDVAVYDGSWTEWGAH 1698
            K++F+QEGISL  P+VTSCGTGVTACILALGLHRLGK+DVAVYDGSWTEWGA+
Sbjct: 312  KKRFDQEGISLKSPVVTSCGTGVTACILALGLHRLGKSDVAVYDGSWTEWGAN 364


>ref|XP_002513368.1| thiosulfate sulfertansferase, putative [Ricinus communis]
            gi|223547276|gb|EEF48771.1| thiosulfate sulfertansferase,
            putative [Ricinus communis]
          Length = 376

 Score =  456 bits (1173), Expect(2) = e-149
 Identities = 227/323 (70%), Positives = 260/323 (80%), Gaps = 1/323 (0%)
 Frame = +3

Query: 495  MAATLMSRTLFATTRILQSSLTPHNTRFFSSSVFKMKPIHYWIAPSYTIRKSFGYFXXXX 674
            MAA   +RTL     I  S       + F S +F  +P +    P +T  ++ G+     
Sbjct: 1    MAAAAFTRTLLGHRFIHCSQSLSLKIQTFPS-LFNKRPFYIRADPVHTSHRTSGWVPRVI 59

Query: 675  XXXXXXXXXX-PVQSMSTSEPVVSVEWLHANLRDPDMKVLDASWYMPNEQRNPLQEYQVA 851
                         QS+  +EPVVSV+WLHANLR+PD+KVLDASWYMP+EQRNP+QEYQVA
Sbjct: 60   ASTMVGTKASYSTQSLPANEPVVSVDWLHANLREPDLKVLDASWYMPDEQRNPIQEYQVA 119

Query: 852  HIPGALFFDVDGISDQATNLPHMLPSEDAFAAAVSALGIENKDGVVVYDGKGIFSAARVW 1031
            HIPGALFFDVDGISD++TNLPHMLPSE+AF+AAVSALGIENKDG+VVYDGKGIFSAARVW
Sbjct: 120  HIPGALFFDVDGISDRSTNLPHMLPSEEAFSAAVSALGIENKDGLVVYDGKGIFSAARVW 179

Query: 1032 WMFRVFGHDNVWVLDGGLPRWRASGFDVESSASSDAILKASAASDAIEKVYHGQTVAPLT 1211
            WMFRVFGH+ VWVLDGGLPRWRASG+DVESSAS DAILKASAAS+AIEKVYHGQ V P+T
Sbjct: 180  WMFRVFGHEKVWVLDGGLPRWRASGYDVESSASGDAILKASAASEAIEKVYHGQAVGPIT 239

Query: 1212 FQAKFQPHLVWNLEQVQRNIEDNSHQLIDARSKPRFDGAAPEPRKGIRSGHVPGSKCIPF 1391
            FQ KFQ HLVW LEQV++NIED +HQ IDARSK RFDGAAPEPRKGIRSGHVPGSKCIPF
Sbjct: 240  FQTKFQLHLVWTLEQVKKNIEDRTHQHIDARSKARFDGAAPEPRKGIRSGHVPGSKCIPF 299

Query: 1392 PQMLDGSQSLLPAESLKEKFEQE 1460
            PQMLD SQ+LLPA+ LK++F+QE
Sbjct: 300  PQMLDASQTLLPADELKKRFDQE 322



 Score =  100 bits (248), Expect(2) = e-149
 Identities = 43/53 (81%), Positives = 49/53 (92%)
 Frame = +1

Query: 1540 KEKFEQEGISLDRPIVTSCGTGVTACILALGLHRLGKTDVAVYDGSWTEWGAH 1698
            K++F+QEGISL+ P+ TSCGTGVTACILALG+HRLGK DV VYDGSWTEWGAH
Sbjct: 316  KKRFDQEGISLESPVFTSCGTGVTACILALGMHRLGKQDVPVYDGSWTEWGAH 368