BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00005706
         (404 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003637458.1| Transcription elongation factor SPT6 [Medica...    67   2e-09
gb|AAC37171.1| cnjB [Tetrahymena thermophila] gi|737494|prf||192...    57   2e-06
ref|XP_001025435.2| Zinc knuckle family protein [Tetrahymena the...    57   2e-06
dbj|BAD99522.1| VASA RNA helicase [Daphnia magna]                      55   8e-06

>ref|XP_003637458.1| Transcription elongation factor SPT6 [Medicago truncatula]
            gi|355503393|gb|AES84596.1| Transcription elongation
            factor SPT6 [Medicago truncatula]
          Length = 2159

 Score = 67.0 bits (162), Expect = 2e-09
 Identities = 60/176 (34%), Positives = 68/176 (38%), Gaps = 42/176 (23%)
 Frame = +3

Query: 3    SSWGVAAPDKEI------GGWSNQALQ----NASNNTPVTETGGWSSSWNKTTANTSKDA 152
            S WG AAP K +       GW     +    N++N    TE GGW       TA ++  A
Sbjct: 1801 SGWG-AAPGKNVTPSGGESGWGGTGGKSWGGNSTNQESNTEKGGWG-----VTAASNGGA 1854

Query: 153  G---SSWGTAVPEKEKESGIWS------SKEGWNISTGTPDKETGGWDXXXXXXXXXXXX 305
            G   S WG+A  +    SG  S      S  GW    GT  K  GG              
Sbjct: 1855 GNENSGWGSANAKNATASGGESKWGETGSGSGWG---GTGGKSWGGSSTNEESNITESGG 1911

Query: 306  XXXXXX------------CYKCGESGHMARECTQ-----------GGSRGGNACYK 404
                              CYKCGESGHMARECTQ           GG RGG ACYK
Sbjct: 1912 SGYGGGGGRGGGRGGGGACYKCGESGHMARECTQEGGGGRGGGGRGGGRGGGACYK 1967



 Score = 59.3 bits (142), Expect = 3e-07
 Identities = 50/160 (31%), Positives = 58/160 (36%), Gaps = 28/160 (17%)
 Frame = +3

Query: 9    WGVAAPDKEIGGWSNQALQNASNNTPVTETGGWSSSWNKTTANTS--KDAGSSWGTAVPE 182
            WGV A     GG  N+     S N       G  S W +T + +      G SWG +   
Sbjct: 1844 WGVTAASN--GGAGNENSGWGSANAKNATASGGESKWGETGSGSGWGGTGGKSWGGS--S 1899

Query: 183  KEKESGIWSSK-EGWNISTGTPDKETGGW-----------------DXXXXXXXXXXXXX 308
              +ES I  S   G+    G      GG                  +             
Sbjct: 1900 TNEESNITESGGSGYGGGGGRGGGRGGGGACYKCGESGHMARECTQEGGGGRGGGGRGGG 1959

Query: 309  XXXXXCYKCGESGHMARECTQ--------GGSRGGNACYK 404
                 CYKCGESGHMARECTQ        GG RGG ACYK
Sbjct: 1960 RGGGACYKCGESGHMARECTQEGGGGGGRGGGRGGGACYK 1999



 Score = 57.0 bits (136), Expect = 2e-06
 Identities = 25/31 (80%), Positives = 25/31 (80%), Gaps = 4/31 (12%)
 Frame = +3

Query: 324  CYKCGESGHMARECTQ----GGSRGGNACYK 404
            CYKCGESGHMARECTQ    GG RGG ACYK
Sbjct: 1547 CYKCGESGHMARECTQEGGGGGGRGGGACYK 1577



 Score = 55.5 bits (132), Expect = 5e-06
 Identities = 25/35 (71%), Positives = 25/35 (71%), Gaps = 8/35 (22%)
 Frame = +3

Query: 324  CYKCGESGHMARECTQ--------GGSRGGNACYK 404
            CYKCGESGHMARECTQ        GG RGG ACYK
Sbjct: 2034 CYKCGESGHMARECTQEGGGGGGRGGGRGGGACYK 2068


>gb|AAC37171.1| cnjB [Tetrahymena thermophila] gi|737494|prf||1922371A cnjB gene
          Length = 1748

 Score = 56.6 bits (135), Expect = 2e-06
 Identities = 46/179 (25%), Positives = 64/179 (35%), Gaps = 45/179 (25%)
 Frame = +3

Query: 3    SSWGVAAPDKEIGGWSNQALQNASNNTPVTETGGWSSSWNKTTAN--TSKDAGSSWGTAV 176
            S WG ++ D            +++N T   E GGW  + NK++ N  + +D  SSWG A 
Sbjct: 1302 SGWGNSSNDSGQNASKEDGWGSSNNKTNSNEGGGWGQNMNKSSDNDWSKQDNSSSWGGAQ 1361

Query: 177  PEK----------EKESGIW------SSKEGWNIS---TGTPDKETGGWDXXXXXXXXXX 299
              K            E G W      SS   W  S   T T +    GW+          
Sbjct: 1362 NNKAPTNNLAKSSSNEGGGWGNNSSSSSSNNWGASKDQTSTNESGGNGWNSSQNNDNSGW 1421

Query: 300  XXXXXXXX---------------------CYKCGESGHMARECT---QGGSRGGNACYK 404
                                         C+KCG+ GHMA++CT   Q G +   AC+K
Sbjct: 1422 GTSNTSSSSNFGQNSGRERNQNGGNKGKGCFKCGKVGHMAKDCTEPQQQGRKQSGACFK 1480


>ref|XP_001025435.2| Zinc knuckle family protein [Tetrahymena thermophila]
            gi|146143687|gb|EAS05190.2| Zinc knuckle family protein
            [Tetrahymena thermophila SB210]
          Length = 1748

 Score = 56.6 bits (135), Expect = 2e-06
 Identities = 46/179 (25%), Positives = 64/179 (35%), Gaps = 45/179 (25%)
 Frame = +3

Query: 3    SSWGVAAPDKEIGGWSNQALQNASNNTPVTETGGWSSSWNKTTAN--TSKDAGSSWGTAV 176
            S WG ++ D            +++N T   E GGW  + NK++ N  + +D  SSWG A 
Sbjct: 1302 SGWGNSSNDSGQNASKEDGWGSSNNKTNSNEGGGWGQNMNKSSDNDWSKQDNSSSWGGAQ 1361

Query: 177  PEK----------EKESGIW------SSKEGWNIS---TGTPDKETGGWDXXXXXXXXXX 299
              K            E G W      SS   W  S   T T +    GW+          
Sbjct: 1362 NNKAPTNNLAKSSSNEGGGWGNNSSSSSSNNWGASKDQTSTNESGGNGWNSSQNNDNSGW 1421

Query: 300  XXXXXXXX---------------------CYKCGESGHMARECT---QGGSRGGNACYK 404
                                         C+KCG+ GHMA++CT   Q G +   AC+K
Sbjct: 1422 GTSNTSSSSNFGQNSGRERNQNGGNKGKGCFKCGKVGHMAKDCTEPQQQGRKQSGACFK 1480


>dbj|BAD99522.1| VASA RNA helicase [Daphnia magna]
          Length = 775

 Score = 54.7 bits (130), Expect = 8e-06
 Identities = 40/125 (32%), Positives = 47/125 (37%), Gaps = 18/125 (14%)
 Frame = +3

Query: 84  PVTETGGWSSSWNKTTANTSKDAGSSWGTAVPEKEKESGIWSSKE--------------- 218
           PV  + GW +   K  A  S D    WG         SG W++ E               
Sbjct: 68  PVPSSNGWDAPAPK--AGNSSD----WGAPASTAPASSGGWNAPESTSQFKNDFDDQPRG 121

Query: 219 -GWNISTGTPDKETGGWDXXXXXXXXXXXXXXXXXXCYKCGESGHMARECTQGGSRGG-- 389
            GW  S     K TGG                    C+KCGE GHMAREC +GG  GG  
Sbjct: 122 GGWGSSGANGLKSTGG-----------------GRPCHKCGEEGHMARECPKGGGGGGGS 164

Query: 390 NACYK 404
            AC+K
Sbjct: 165 RACHK 169