BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00005673
         (1221 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAL69911.1| obtusifoliol-14-demethylase [Petunia x hybrida]       656   0.0  
gb|AAT12274.1| obtusifoliol 14alpha-demethylase [Solanum chacoense]   655   0.0  
gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana ...   655   0.0  
ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum ly...   654   0.0  
gb|AAL40888.1| obtusifoliol-14-demethylase [Nicotiana tabacum]        652   0.0  

>emb|CAL69911.1| obtusifoliol-14-demethylase [Petunia x hybrida]
          Length = 492

 Score =  656 bits (1692), Expect = 0.0
 Identities = 324/406 (79%), Positives = 355/406 (87%)
 Frame = -2

Query: 1220 PIVMLREEYPKLGSVFTLNLVNKKITFLIGPEVSSHFFKAVETDLSQQEVYRFNVPTFGP 1041
            P+VMLREEYPKLGSVFTLNL+NKKITF IGPEVS+HFFKA ETDLSQQEVY+FNVPTFGP
Sbjct: 58   PVVMLREEYPKLGSVFTLNLLNKKITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGP 117

Query: 1040 GVVFDVDYSIRQEQFRFFTDSLRVSKLKSYVDQMVVEAEDYFAKWGDSGEVDLKHELEQL 861
            GVVFDVDY+IRQEQFRFFT+SLRV+KLK YVDQMV+EAE+YF+KWGDSGEVDLK+ELE L
Sbjct: 118  GVVFDVDYTIRQEQFRFFTESLRVTKLKGYVDQMVMEAEEYFSKWGDSGEVDLKYELEHL 177

Query: 860  IILTASRCLLGIEVRNKLFDDVSALFHDLDSGMLPISVIFPYLPIPAHRRRDQAREKLAE 681
            IILTASRCLLG EVRNKLF+DVSALFHDLD+GMLPISVIFPYL IPAHRRRD AR+KLA+
Sbjct: 178  IILTASRCLLGEEVRNKLFEDVSALFHDLDNGMLPISVIFPYLSIPAHRRRDNARKKLAQ 237

Query: 680  IFGRIINSRKQAGKSENDMLQSFMDSKYKNGRPTTDTEVTGLLIAALFAGQHTSSITSTW 501
            IF  II SRK+ GK+E+DMLQ F+DSKYK+GRPTT++E+TGLLIAALFAGQHTSSITSTW
Sbjct: 238  IFANIIASRKRTGKAESDMLQCFIDSKYKDGRPTTESEITGLLIAALFAGQHTSSITSTW 297

Query: 500  TGAYLLANKKYLSAVSDEQKTIMRKHGNNVDHDILSEMEALHRCIKEALRLHPPLIMLLR 321
            TGAYLL N KY+SAV DEQK +M+KHGN VDHDILSEME L+RCIKEALRLHPPLIMLLR
Sbjct: 298  TGAYLLCNNKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLR 357

Query: 320  SSHSDFSVKTREGKEYDIPKGHIVATSPAFAXXXXXXXXXXXXXXXXXXXXXXXXXXPAF 141
            SSHSDF+VKTREGKEYDIPKGHIVATS                              PAF
Sbjct: 358  SSHSDFTVKTREGKEYDIPKGHIVATS------------------------------PAF 387

Query: 140  ANRLPHIYKDPDTFDPDRFGPGRDEDKAAGPFSYISFGGGRHGCLG 3
            ANRLPHIYK+PDT+DPDRF PGR+EDK AG FSYISFGGGRHGCLG
Sbjct: 388  ANRLPHIYKNPDTYDPDRFAPGREEDKTAGAFSYISFGGGRHGCLG 433


>gb|AAT12274.1| obtusifoliol 14alpha-demethylase [Solanum chacoense]
          Length = 487

 Score =  655 bits (1691), Expect = 0.0
 Identities = 323/406 (79%), Positives = 355/406 (87%)
 Frame = -2

Query: 1220 PIVMLREEYPKLGSVFTLNLVNKKITFLIGPEVSSHFFKAVETDLSQQEVYRFNVPTFGP 1041
            PIVMLR+EYPKLGSVFTLNL+NK ITF IGPEVS+HFFKA ETDLSQQEVY+FNVPTFGP
Sbjct: 57   PIVMLRQEYPKLGSVFTLNLLNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGP 116

Query: 1040 GVVFDVDYSIRQEQFRFFTDSLRVSKLKSYVDQMVVEAEDYFAKWGDSGEVDLKHELEQL 861
            GVVFDVDY+IRQEQFRFFT++LRV+KLK YVDQMV EAE+YF+KWG+SGEVDLK+ELE L
Sbjct: 117  GVVFDVDYTIRQEQFRFFTEALRVTKLKGYVDQMVTEAEEYFSKWGESGEVDLKYELEHL 176

Query: 860  IILTASRCLLGIEVRNKLFDDVSALFHDLDSGMLPISVIFPYLPIPAHRRRDQAREKLAE 681
            IILTASRCLLG EVRNKLFDDVSALFHDLD+GMLPISVIFPYLPIPAHRRRD AR+KLAE
Sbjct: 177  IILTASRCLLGEEVRNKLFDDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAE 236

Query: 680  IFGRIINSRKQAGKSENDMLQSFMDSKYKNGRPTTDTEVTGLLIAALFAGQHTSSITSTW 501
            IF  IINSRK+ GK+ENDMLQ F+DSKYK+GRPTT+ E+TGLLIAALFAGQHTSSITSTW
Sbjct: 237  IFANIINSRKRTGKAENDMLQCFIDSKYKDGRPTTEGEITGLLIAALFAGQHTSSITSTW 296

Query: 500  TGAYLLANKKYLSAVSDEQKTIMRKHGNNVDHDILSEMEALHRCIKEALRLHPPLIMLLR 321
            TG+YLL N KY+SAV DEQK +M+KHGN VDHDILSEM+ L+RCIKEALRLHPPLIMLLR
Sbjct: 297  TGSYLLTNNKYMSAVVDEQKNLMKKHGNKVDHDILSEMDVLYRCIKEALRLHPPLIMLLR 356

Query: 320  SSHSDFSVKTREGKEYDIPKGHIVATSPAFAXXXXXXXXXXXXXXXXXXXXXXXXXXPAF 141
            SSHSDFSV TREGKEYDIPKGHIVATS                              PAF
Sbjct: 357  SSHSDFSVTTREGKEYDIPKGHIVATS------------------------------PAF 386

Query: 140  ANRLPHIYKDPDTFDPDRFGPGRDEDKAAGPFSYISFGGGRHGCLG 3
            ANRLPHI+K+P+++DPDRFGPGR+EDKAAG FSYISFGGGRHGCLG
Sbjct: 387  ANRLPHIFKNPESYDPDRFGPGREEDKAAGAFSYISFGGGRHGCLG 432


>gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana tabacum]
          Length = 487

 Score =  655 bits (1690), Expect = 0.0
 Identities = 322/406 (79%), Positives = 354/406 (87%)
 Frame = -2

Query: 1220 PIVMLREEYPKLGSVFTLNLVNKKITFLIGPEVSSHFFKAVETDLSQQEVYRFNVPTFGP 1041
            P+VMLREEYPKLGSVFTLNL+NK ITF +GPEVS+HFFKA ETDLSQQEVY+FNVPTFGP
Sbjct: 57   PVVMLREEYPKLGSVFTLNLLNKNITFFVGPEVSAHFFKAPETDLSQQEVYQFNVPTFGP 116

Query: 1040 GVVFDVDYSIRQEQFRFFTDSLRVSKLKSYVDQMVVEAEDYFAKWGDSGEVDLKHELEQL 861
            GVVFDVDY+IRQEQFRFFT+SLRV+KLK YVDQMV+EAE+YF+KWGDSGEVDLK+ELE L
Sbjct: 117  GVVFDVDYTIRQEQFRFFTESLRVNKLKGYVDQMVMEAEEYFSKWGDSGEVDLKYELEHL 176

Query: 860  IILTASRCLLGIEVRNKLFDDVSALFHDLDSGMLPISVIFPYLPIPAHRRRDQAREKLAE 681
            IILTASRCLLG EVRNKLF+DVSALFHDLD+GMLPISVIFPYLPIPAHRRRD AR+KLAE
Sbjct: 177  IILTASRCLLGEEVRNKLFEDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAE 236

Query: 680  IFGRIINSRKQAGKSENDMLQSFMDSKYKNGRPTTDTEVTGLLIAALFAGQHTSSITSTW 501
            IF  II+SRK+ GK+E+DMLQ F+DSKYK+GR TT++E+TGLLIAALFAGQHTSSITSTW
Sbjct: 237  IFANIIDSRKRTGKAESDMLQCFIDSKYKDGRATTESEITGLLIAALFAGQHTSSITSTW 296

Query: 500  TGAYLLANKKYLSAVSDEQKTIMRKHGNNVDHDILSEMEALHRCIKEALRLHPPLIMLLR 321
             GAYLL N KY+SAV DEQK +M+KHGN VDHDILSEME L+RCIKEALRLHPPLIMLLR
Sbjct: 297  AGAYLLCNNKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLR 356

Query: 320  SSHSDFSVKTREGKEYDIPKGHIVATSPAFAXXXXXXXXXXXXXXXXXXXXXXXXXXPAF 141
            SSHSDF+VKTREGKEYDIPKGHIVATS                              PAF
Sbjct: 357  SSHSDFTVKTREGKEYDIPKGHIVATS------------------------------PAF 386

Query: 140  ANRLPHIYKDPDTFDPDRFGPGRDEDKAAGPFSYISFGGGRHGCLG 3
            ANRLPH+YK+PDT+DPDRF PGRDEDK AG FSYISFGGGRHGCLG
Sbjct: 387  ANRLPHVYKNPDTYDPDRFAPGRDEDKVAGAFSYISFGGGRHGCLG 432


>ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum lycopersicum]
            gi|299735181|gb|ADJ37071.1| obtusifoliol
            14alpha-demethylase [Solanum lycopersicum]
          Length = 487

 Score =  654 bits (1688), Expect = 0.0
 Identities = 323/406 (79%), Positives = 354/406 (87%)
 Frame = -2

Query: 1220 PIVMLREEYPKLGSVFTLNLVNKKITFLIGPEVSSHFFKAVETDLSQQEVYRFNVPTFGP 1041
            P+VMLR+EYPKLGSVFTLNL+NK ITF IGPEVS+HFFKA ETDLSQQEVY+FNVPTFGP
Sbjct: 57   PVVMLRQEYPKLGSVFTLNLLNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGP 116

Query: 1040 GVVFDVDYSIRQEQFRFFTDSLRVSKLKSYVDQMVVEAEDYFAKWGDSGEVDLKHELEQL 861
            GVVFDVDY+IRQEQFRFFT++LRV+KLK YVDQMV EAE+YF+KWG+SGEVDLK+ELE L
Sbjct: 117  GVVFDVDYTIRQEQFRFFTEALRVTKLKGYVDQMVTEAEEYFSKWGESGEVDLKYELEHL 176

Query: 860  IILTASRCLLGIEVRNKLFDDVSALFHDLDSGMLPISVIFPYLPIPAHRRRDQAREKLAE 681
            IILTASRCLLG EVRNKLFDDVSALFHDLD+GMLPISVIFPYLPIPAHRRRD AR+KLAE
Sbjct: 177  IILTASRCLLGEEVRNKLFDDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAE 236

Query: 680  IFGRIINSRKQAGKSENDMLQSFMDSKYKNGRPTTDTEVTGLLIAALFAGQHTSSITSTW 501
            IF  IINSRK+ GK+ENDMLQ F+DSKYK+GRPTT+ E+TGLLIAALFAGQHTSSITSTW
Sbjct: 237  IFANIINSRKRTGKAENDMLQCFIDSKYKDGRPTTEGEITGLLIAALFAGQHTSSITSTW 296

Query: 500  TGAYLLANKKYLSAVSDEQKTIMRKHGNNVDHDILSEMEALHRCIKEALRLHPPLIMLLR 321
            TG+YLL N KY+SAV DEQK +M+KHGN VDHDILSEME L+RCIKEALRLHPPLIMLLR
Sbjct: 297  TGSYLLTNDKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLR 356

Query: 320  SSHSDFSVKTREGKEYDIPKGHIVATSPAFAXXXXXXXXXXXXXXXXXXXXXXXXXXPAF 141
            SSHS+FSV TREGKEYDI KGHIVATS                              PAF
Sbjct: 357  SSHSEFSVTTREGKEYDISKGHIVATS------------------------------PAF 386

Query: 140  ANRLPHIYKDPDTFDPDRFGPGRDEDKAAGPFSYISFGGGRHGCLG 3
            ANRLPHI+K+PDT+DPDRFGPGR+EDKAAG FSYISFGGGRHGCLG
Sbjct: 387  ANRLPHIFKNPDTYDPDRFGPGREEDKAAGAFSYISFGGGRHGCLG 432


>gb|AAL40888.1| obtusifoliol-14-demethylase [Nicotiana tabacum]
          Length = 487

 Score =  652 bits (1683), Expect = 0.0
 Identities = 321/406 (79%), Positives = 353/406 (86%)
 Frame = -2

Query: 1220 PIVMLREEYPKLGSVFTLNLVNKKITFLIGPEVSSHFFKAVETDLSQQEVYRFNVPTFGP 1041
            P+VMLREEYPKLGSVFTLNL+NK ITF IGPEVS+HFFKA ETDLSQQEVY+FNVPTFGP
Sbjct: 57   PVVMLREEYPKLGSVFTLNLLNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGP 116

Query: 1040 GVVFDVDYSIRQEQFRFFTDSLRVSKLKSYVDQMVVEAEDYFAKWGDSGEVDLKHELEQL 861
            GVVFDVDY+IRQEQFRFFT++LRV+KLK YVD MV+EAE+YF+KWGDSGE+DLK+ELE L
Sbjct: 117  GVVFDVDYTIRQEQFRFFTEALRVNKLKGYVDHMVMEAEEYFSKWGDSGEMDLKYELEHL 176

Query: 860  IILTASRCLLGIEVRNKLFDDVSALFHDLDSGMLPISVIFPYLPIPAHRRRDQAREKLAE 681
            IILTASRCLLG EVRNKLF+DVSALFHDLD+GMLPISVIFPYLPIPAHRRRD AR+KLAE
Sbjct: 177  IILTASRCLLGEEVRNKLFEDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAE 236

Query: 680  IFGRIINSRKQAGKSENDMLQSFMDSKYKNGRPTTDTEVTGLLIAALFAGQHTSSITSTW 501
            IF  II+SRK+ GK+E+DMLQ F+DSKYK+GR TTD+E+TGLLIAALFAGQHTSSITSTW
Sbjct: 237  IFANIIDSRKRTGKAESDMLQCFIDSKYKDGRATTDSEITGLLIAALFAGQHTSSITSTW 296

Query: 500  TGAYLLANKKYLSAVSDEQKTIMRKHGNNVDHDILSEMEALHRCIKEALRLHPPLIMLLR 321
            TGAYLL N KY+SAV DEQK +M+KHGN VDHDILSEME L+RCIKE LRLHPPLIMLLR
Sbjct: 297  TGAYLLCNNKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEVLRLHPPLIMLLR 356

Query: 320  SSHSDFSVKTREGKEYDIPKGHIVATSPAFAXXXXXXXXXXXXXXXXXXXXXXXXXXPAF 141
            SSHSDF+VKTREGKEYDIPKGHIVATS                              PAF
Sbjct: 357  SSHSDFTVKTREGKEYDIPKGHIVATS------------------------------PAF 386

Query: 140  ANRLPHIYKDPDTFDPDRFGPGRDEDKAAGPFSYISFGGGRHGCLG 3
            ANRLPH+YK+PDT+DPDRF PGRDEDK AG FSYISFGGGRHGCLG
Sbjct: 387  ANRLPHVYKNPDTYDPDRFTPGRDEDKVAGAFSYISFGGGRHGCLG 432