BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00005661
         (1677 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002281321.1| PREDICTED: IAA-amino acid hydrolase ILR1-lik...   641   0.0  
ref|XP_002303219.1| iaa-amino acid hydrolase 11 [Populus trichoc...   637   e-180
emb|CAN70580.1| hypothetical protein VITISV_033718 [Vitis vinifera]   637   e-180
gb|AFR58665.1| jasmonoyl-L-isoleucine hydrolase 1 [Nicotiana att...   632   e-179
emb|CAG32961.1| putative auxin-amidohydrolase precursor [Populus...   629   e-178

>ref|XP_002281321.1| PREDICTED: IAA-amino acid hydrolase ILR1-like 4 [Vitis vinifera]
            gi|297743123|emb|CBI35990.3| unnamed protein product
            [Vitis vinifera]
          Length = 441

 Score =  641 bits (1654), Expect = 0.0
 Identities = 314/444 (70%), Positives = 371/444 (83%)
 Frame = -2

Query: 1592 MDLVKWVFMISTFCIFVPTPTLSDSFLKEGLTEIPINFLNLAKRSDLVDYMVKIRRKIHE 1413
            M   +WV  I   C+F PTP  S+S L    + IP NFL+ A++ ++VD++V +RRKIHE
Sbjct: 1    MTYTEWVSWIFILCLFGPTPISSESSLS---SNIPTNFLSFARKQEVVDWLVGVRRKIHE 57

Query: 1412 NPELGFEEFETSKFIRSELDDMGIQYKHPFAVTGVVGFIGSGDPPFVALRADMDALAMQE 1233
            NPELGFEE ETSK +R+ELD MGI YK+P AVTGV+GF+G+G+PPFVA+RADMDALAMQE
Sbjct: 58   NPELGFEEVETSKLVRAELDKMGIPYKYPVAVTGVLGFVGTGEPPFVAIRADMDALAMQE 117

Query: 1232 GVEWEHKSKVPGKMHACGHDAHVAMLLGAAKMLQEHHNDIQGTIVLVFQPAEEGGAGAKK 1053
             VEWEHKSK+PGKMHACGHD+HVAMLLGAAK+LQEH  ++QGT++LVFQPAEEGG GAKK
Sbjct: 118  MVEWEHKSKIPGKMHACGHDSHVAMLLGAAKILQEHREELQGTVILVFQPAEEGGGGAKK 177

Query: 1052 MLDEGALQNVEAIFGLHISSRFPIGKVATRPGPILAGSGFFEAVISGKGGHAAIPQHTID 873
            +LD G L+NV AIFGLH+S   PIG+VA+R GP+LAGSGFFEAVISGKGGHAAIPQH+ID
Sbjct: 178  ILDAGVLENVNAIFGLHVSPDLPIGEVASRSGPLLAGSGFFEAVISGKGGHAAIPQHSID 237

Query: 872  PILAASNVVVSLQHLVSREADPLDSQVVTVGKFQGGEAFNVIPDSVTIGGTFRTFSIESF 693
            PILAASNV+VSLQHLVSREADPL+SQVVTV KFQGG AFNVIPDSVTIGGTFR FS ES 
Sbjct: 238  PILAASNVIVSLQHLVSREADPLESQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKESI 297

Query: 692  EKLKRRITEVITGQAAVQRCNASVNFNAQGKTFYPVTVNNKELHKHFQKVVKDMLGVENL 513
             +LK+RI EVIT QAAVQRCNA+V+F+ + K  +P T+NN  LHKHFQ VV +MLGV N+
Sbjct: 298  MQLKQRIEEVITRQAAVQRCNATVDFHEKEKPLFPATINNPNLHKHFQNVVGNMLGVHNV 357

Query: 512  KDMQPLMGAEDFSFFAEAIPGYFYFVGMHNETQGRPESGHSPYFTINEDALPYGAALHAS 333
            KDMQPLMG+EDFSF+ E +PGYF+F+GM +E  GR  S HSP+F INEDALPYGAALHAS
Sbjct: 358  KDMQPLMGSEDFSFYQEEMPGYFFFLGMKDEALGRLPSVHSPHFKINEDALPYGAALHAS 417

Query: 332  VATRYLLEKQPKLISQDKSVRDEL 261
            +A  YLLE QP+  S++    DEL
Sbjct: 418  LAATYLLEIQPQPSSREGKHHDEL 441


>ref|XP_002303219.1| iaa-amino acid hydrolase 11 [Populus trichocarpa]
            gi|222840651|gb|EEE78198.1| iaa-amino acid hydrolase 11
            [Populus trichocarpa]
          Length = 438

 Score =  637 bits (1643), Expect = e-180
 Identities = 311/444 (70%), Positives = 373/444 (84%)
 Frame = -2

Query: 1592 MDLVKWVFMISTFCIFVPTPTLSDSFLKEGLTEIPINFLNLAKRSDLVDYMVKIRRKIHE 1413
            M L  WV +   F +++ +P LS      G ++IP  FLN AK+ +L D+MV +RRKIHE
Sbjct: 1    MSLFNWVSL--GFFLYLLSPILS----LNGSSDIPSRFLNYAKKEELFDWMVGVRRKIHE 54

Query: 1412 NPELGFEEFETSKFIRSELDDMGIQYKHPFAVTGVVGFIGSGDPPFVALRADMDALAMQE 1233
            NPELGFEEFETSK +R+ELD +G++YKHP +VTGVVGFIGSG PPFVALRADMDALAMQE
Sbjct: 55   NPELGFEEFETSKLVRAELDKIGVKYKHPLSVTGVVGFIGSGKPPFVALRADMDALAMQE 114

Query: 1232 GVEWEHKSKVPGKMHACGHDAHVAMLLGAAKMLQEHHNDIQGTIVLVFQPAEEGGAGAKK 1053
             VEWE+KSKVPGKMHACGHD+HVAMLLGAAK+LQ+H  +++GT+VL+FQPAEEGG GAKK
Sbjct: 115  MVEWEYKSKVPGKMHACGHDSHVAMLLGAAKILQDHREELKGTVVLIFQPAEEGGGGAKK 174

Query: 1052 MLDEGALQNVEAIFGLHISSRFPIGKVATRPGPILAGSGFFEAVISGKGGHAAIPQHTID 873
            M+DEGAL+NV AIFGLH++++ PIG+VA+R GP+LAGSGFFEAVISGKGGHAAIPQH+ID
Sbjct: 175  MIDEGALENVNAIFGLHVANKLPIGEVASRHGPLLAGSGFFEAVISGKGGHAAIPQHSID 234

Query: 872  PILAASNVVVSLQHLVSREADPLDSQVVTVGKFQGGEAFNVIPDSVTIGGTFRTFSIESF 693
            PILAASNV+VSLQHLVSREADPLDSQVVTV KFQGG AFNVIPDSVTIGGTFR F  ESF
Sbjct: 235  PILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKESF 294

Query: 692  EKLKRRITEVITGQAAVQRCNASVNFNAQGKTFYPVTVNNKELHKHFQKVVKDMLGVENL 513
             +LK+RI EV+TGQAAVQRC A +NF    K F+P T+N+K LH +F+ V  DMLG++ +
Sbjct: 295  MQLKQRIEEVVTGQAAVQRCKAVINFLENEKPFFPPTINDKYLHDYFRIVASDMLGIDKV 354

Query: 512  KDMQPLMGAEDFSFFAEAIPGYFYFVGMHNETQGRPESGHSPYFTINEDALPYGAALHAS 333
            KDMQPLMG+EDF+F+ E IPGYF+F+GM NET  + +S HSPYF INED LPYGAALHAS
Sbjct: 355  KDMQPLMGSEDFAFYQEMIPGYFFFIGMQNETHKQLQSPHSPYFEINEDVLPYGAALHAS 414

Query: 332  VATRYLLEKQPKLISQDKSVRDEL 261
            +A RYLLE QP++   +++  DEL
Sbjct: 415  LAARYLLEFQPEVTLPEENDHDEL 438


>emb|CAN70580.1| hypothetical protein VITISV_033718 [Vitis vinifera]
          Length = 441

 Score =  637 bits (1643), Expect = e-180
 Identities = 312/444 (70%), Positives = 369/444 (83%)
 Frame = -2

Query: 1592 MDLVKWVFMISTFCIFVPTPTLSDSFLKEGLTEIPINFLNLAKRSDLVDYMVKIRRKIHE 1413
            M   +WV  I   C+F PTP  S+S L    + IP NFL+ A++ ++VD++V +RRKIHE
Sbjct: 1    MTYTEWVSWIFILCLFGPTPISSESSLS---SNIPTNFLSFARKQEVVDWLVGVRRKIHE 57

Query: 1412 NPELGFEEFETSKFIRSELDDMGIQYKHPFAVTGVVGFIGSGDPPFVALRADMDALAMQE 1233
            NPELGFEE ETSK +R+ELD MGI YK+P AVTGV+GF+G+G+PPFVA+RADMDALAMQE
Sbjct: 58   NPELGFEEVETSKLVRAELDKMGIPYKYPVAVTGVLGFVGTGEPPFVAIRADMDALAMQE 117

Query: 1232 GVEWEHKSKVPGKMHACGHDAHVAMLLGAAKMLQEHHNDIQGTIVLVFQPAEEGGAGAKK 1053
             VEWEHKSK+PGKMHACGHD+HVAMLLGAAK+LQEH  ++QGT++LVFQPAEEGG GAKK
Sbjct: 118  MVEWEHKSKIPGKMHACGHDSHVAMLLGAAKILQEHREELQGTVILVFQPAEEGGGGAKK 177

Query: 1052 MLDEGALQNVEAIFGLHISSRFPIGKVATRPGPILAGSGFFEAVISGKGGHAAIPQHTID 873
            +LD G L+NV AIFGLH+S   PIG+VA+R GP+LAGSGFFEAVISGKGGHAAIPQH+ID
Sbjct: 178  ILDAGVLENVNAIFGLHVSPDLPIGEVASRSGPLLAGSGFFEAVISGKGGHAAIPQHSID 237

Query: 872  PILAASNVVVSLQHLVSREADPLDSQVVTVGKFQGGEAFNVIPDSVTIGGTFRTFSIESF 693
            PILAASNV+VSLQHLVSREADPL+SQVVTV KFQGG AFNVIPDSVTIGGTFR FS ES 
Sbjct: 238  PILAASNVIVSLQHLVSREADPLESQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKESI 297

Query: 692  EKLKRRITEVITGQAAVQRCNASVNFNAQGKTFYPVTVNNKELHKHFQKVVKDMLGVENL 513
             +LK+RI EVIT QAAVQRCNA+V+F+ + K  +P T+NN  LHKHFQ V  +MLGV N+
Sbjct: 298  MQLKQRIEEVITRQAAVQRCNATVDFHEKEKPLFPATINNPNLHKHFQNVAGNMLGVHNV 357

Query: 512  KDMQPLMGAEDFSFFAEAIPGYFYFVGMHNETQGRPESGHSPYFTINEDALPYGAALHAS 333
            KDMQPLMG+EDFSF+ E +PGYF+F+GM +E  GR  S HSP+F INE ALPYGAALHAS
Sbjct: 358  KDMQPLMGSEDFSFYQEEMPGYFFFLGMKDEALGRLPSVHSPHFKINEGALPYGAALHAS 417

Query: 332  VATRYLLEKQPKLISQDKSVRDEL 261
            +A  YLLE QP+  S++    DEL
Sbjct: 418  LAATYLLEIQPQPSSREGKHHDEL 441


>gb|AFR58665.1| jasmonoyl-L-isoleucine hydrolase 1 [Nicotiana attenuata]
          Length = 441

 Score =  632 bits (1630), Expect = e-179
 Identities = 309/439 (70%), Positives = 368/439 (83%)
 Frame = -2

Query: 1592 MDLVKWVFMISTFCIFVPTPTLSDSFLKEGLTEIPINFLNLAKRSDLVDYMVKIRRKIHE 1413
            MD  +WVF+I  F  F   P  SDS     L+EIPINFLN AK++++ D++V +RR+IHE
Sbjct: 1    MDFSRWVFLILIFVSFSAIPIWSDS----SLSEIPINFLNFAKKAEVFDWIVGVRRRIHE 56

Query: 1412 NPELGFEEFETSKFIRSELDDMGIQYKHPFAVTGVVGFIGSGDPPFVALRADMDALAMQE 1233
            NPELG+EEFETSK IR ELD +GI YK+PFA TG+VGF+GSG  PFVA+RADMDAL MQE
Sbjct: 57   NPELGYEEFETSKIIREELDKLGISYKYPFATTGIVGFVGSGKSPFVAIRADMDALPMQE 116

Query: 1232 GVEWEHKSKVPGKMHACGHDAHVAMLLGAAKMLQEHHNDIQGTIVLVFQPAEEGGAGAKK 1053
             V+WEHKSK  GKMHACGHDAHVAMLLGAAK+LQEH + ++GT+ LVFQPAEEGG GAKK
Sbjct: 117  MVDWEHKSKNAGKMHACGHDAHVAMLLGAAKILQEHRDILKGTVALVFQPAEEGGGGAKK 176

Query: 1052 MLDEGALQNVEAIFGLHISSRFPIGKVATRPGPILAGSGFFEAVISGKGGHAAIPQHTID 873
            M+D GAL+N+E+IFGLH++ +FP+GKV++RPGP LAGSGFFEAVISGKGGHAAIPQH+ID
Sbjct: 177  MIDAGALENIESIFGLHVNPQFPLGKVSSRPGPFLAGSGFFEAVISGKGGHAAIPQHSID 236

Query: 872  PILAASNVVVSLQHLVSREADPLDSQVVTVGKFQGGEAFNVIPDSVTIGGTFRTFSIESF 693
            PILAASNV+VSLQHLVSREADPLDSQVVTV KFQGG AFNVIPDSVTIGGTFR FS ESF
Sbjct: 237  PILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKESF 296

Query: 692  EKLKRRITEVITGQAAVQRCNASVNFNAQGKTFYPVTVNNKELHKHFQKVVKDMLGVENL 513
            ++L++RI EVI GQAAVQRCNA+V+F  + K F+P TVN+K LHKHFQ+V  DMLG +++
Sbjct: 297  QQLRQRIEEVIVGQAAVQRCNATVDFLTKEKPFFPPTVNDKNLHKHFQRVAGDMLGNDHV 356

Query: 512  KDMQPLMGAEDFSFFAEAIPGYFYFVGMHNETQGRPESGHSPYFTINEDALPYGAALHAS 333
            KDM+PLMG+EDF+F+ E IPGYFY +GM +ET  +  S HSPYF INE+ALP GAAL AS
Sbjct: 357  KDMEPLMGSEDFAFYQEVIPGYFYLLGMQDETNEKLVSVHSPYFKINEEALPIGAALQAS 416

Query: 332  VATRYLLEKQPKLISQDKS 276
            +A RYLLE Q ++ S   S
Sbjct: 417  LAIRYLLEAQSQVPSSSIS 435


>emb|CAG32961.1| putative auxin-amidohydrolase precursor [Populus tremula x Populus
            alba]
          Length = 438

 Score =  629 bits (1623), Expect = e-178
 Identities = 307/444 (69%), Positives = 372/444 (83%)
 Frame = -2

Query: 1592 MDLVKWVFMISTFCIFVPTPTLSDSFLKEGLTEIPINFLNLAKRSDLVDYMVKIRRKIHE 1413
            M    WV +   F +++ +P LS      G ++IP  FLN AK+ +L D+MV +RRKIHE
Sbjct: 1    MSWFNWVSL--GFFLYLLSPILS----LNGSSDIPSRFLNHAKKEELFDWMVGVRRKIHE 54

Query: 1412 NPELGFEEFETSKFIRSELDDMGIQYKHPFAVTGVVGFIGSGDPPFVALRADMDALAMQE 1233
            NPELG+EEFETSK IR+ELD +G++YKHP +VTGVVGFIGSG+PPFVALRADMDALAMQE
Sbjct: 55   NPELGYEEFETSKLIRAELDKIGVKYKHPLSVTGVVGFIGSGEPPFVALRADMDALAMQE 114

Query: 1232 GVEWEHKSKVPGKMHACGHDAHVAMLLGAAKMLQEHHNDIQGTIVLVFQPAEEGGAGAKK 1053
             VEWE+KSKVPGKMHACGHD+HVAMLLGAAK+LQ+H  +++GT+VL+FQPAEEGG GAKK
Sbjct: 115  MVEWEYKSKVPGKMHACGHDSHVAMLLGAAKILQDHREELKGTVVLIFQPAEEGGGGAKK 174

Query: 1052 MLDEGALQNVEAIFGLHISSRFPIGKVATRPGPILAGSGFFEAVISGKGGHAAIPQHTID 873
            M+DEGAL+NV AIFGLH++++ PIG+VA+R GP+LAGSGFFEAVISGKGGHAAIPQH+ID
Sbjct: 175  MIDEGALENVNAIFGLHVANKLPIGEVASRHGPLLAGSGFFEAVISGKGGHAAIPQHSID 234

Query: 872  PILAASNVVVSLQHLVSREADPLDSQVVTVGKFQGGEAFNVIPDSVTIGGTFRTFSIESF 693
            PILAASNV+VSLQHLVSREADPLDSQVVTV KFQGG AFNVIPDSVT GGTFR F  ESF
Sbjct: 235  PILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTTGGTFRAFLKESF 294

Query: 692  EKLKRRITEVITGQAAVQRCNASVNFNAQGKTFYPVTVNNKELHKHFQKVVKDMLGVENL 513
             +L++RI EV+TGQAAVQRC A +N     K F+P T+N+K LH +F+ V  D+LG++ +
Sbjct: 295  MQLRQRIEEVVTGQAAVQRCKAVINLLENEKPFFPPTINDKNLHDYFRVVASDVLGIDKV 354

Query: 512  KDMQPLMGAEDFSFFAEAIPGYFYFVGMHNETQGRPESGHSPYFTINEDALPYGAALHAS 333
            KDMQPLMG+EDF+F+ E IPGYF+FVGM NET+ + +S HSPYF INED LPYGAALHAS
Sbjct: 355  KDMQPLMGSEDFAFYQEKIPGYFFFVGMQNETRKQLQSPHSPYFEINEDVLPYGAALHAS 414

Query: 332  VATRYLLEKQPKLISQDKSVRDEL 261
            +A RYLLE QP++   +++  DEL
Sbjct: 415  LAARYLLEFQPQVTLPEENDHDEL 438