BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00005558
         (609 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|C0SJS3.1|ANGS_PASSA RecName: Full=Angelicin synthase; AltName...   197   9e-49
sp|Q6QNI4.1|C71AJ_AMMMJ RecName: Full=Psoralen synthase; AltName...   182   3e-44
sp|C0SJS4.1|C71AJ_APIGR RecName: Full=Psoralen synthase; AltName...   179   4e-43
sp|C0SJS2.1|C71AJ_PASSA RecName: Full=Psoralen synthase; AltName...   178   8e-43
gb|ABC69416.1| CYP71AU1 [Nicotiana tabacum]                           153   3e-35

>sp|C0SJS3.1|ANGS_PASSA RecName: Full=Angelicin synthase; AltName: Full=Cytochrome P450
           CYP71AJ4 gi|140083745|gb|ABO84854.1| cytochrome P450
           [Pastinaca sativa]
          Length = 478

 Score =  197 bits (502), Expect = 9e-49
 Identities = 107/205 (52%), Positives = 138/205 (67%), Gaps = 3/205 (1%)
 Frame = +2

Query: 2   VDLLIQNLQYSDQNIINLMDMLSELANNVVCRVSLGRKF--SDKGNSYNKLLAEVTNLLG 175
           V LLIQN++ S   +INL + L +L  NVVCRVS+G ++    KG  Y KLLAEVT +L 
Sbjct: 145 VALLIQNIENSASEVINLGEQLIQLTRNVVCRVSVGSEYLSGHKGKLYQKLLAEVTEMLA 204

Query: 176 LR-SIGDYIPVLCWXXXXXXXXXXXXXXXXXXXXFLEGVLKDHQNTIASDDGCGNGNFIS 352
              SIGD+IP+L W                    FLEG L+DH  T+AS+ G  N +F+S
Sbjct: 205 YTYSIGDFIPLLGWVDWLSGSKAKVEKTAKEVDAFLEGALRDHIKTMASNKGSANDDFLS 264

Query: 353 ILLDVQKQNTDNSFSIDKDCIKAVILDMFFAGTDTTSTTLEWTIAALIKSPDAMFKLQKE 532
           ILL++++   D   ++D++CIKA++ DM   GT+TTSTTLEW +AA+IK+PD MFKLQKE
Sbjct: 265 ILLEIRE--ADAGSTLDEECIKAIVWDMILGGTETTSTTLEWIVAAIIKNPDVMFKLQKE 322

Query: 533 VRERGKGKSKISEGDLVNMHYLKAV 607
           VRE GKGKSKI E DLV M+YLKAV
Sbjct: 323 VREIGKGKSKIEEVDLVKMNYLKAV 347


>sp|Q6QNI4.1|C71AJ_AMMMJ RecName: Full=Psoralen synthase; AltName: Full=Cytochrome P450
           CYP71AJ1 gi|46947673|gb|AAT06911.1| psoralen synthase
           [Ammi majus]
          Length = 494

 Score =  182 bits (463), Expect = 3e-44
 Identities = 104/203 (51%), Positives = 134/203 (66%), Gaps = 1/203 (0%)
 Frame = +2

Query: 2   VDLLIQNLQYSDQNIINLMDMLSELANNVVCRVSLGRKFSDKGNSYNKLLAEVTNLLGL- 178
           VDLL+QNL+ S   + NL ++L E+  NVVCRVS+G    DK +SY  L+ E+ ++LG  
Sbjct: 157 VDLLVQNLENSHSKVANLTELLIEVTGNVVCRVSVGS--GDKVDSYKILILEIMDMLGYS 214

Query: 179 RSIGDYIPVLCWXXXXXXXXXXXXXXXXXXXXFLEGVLKDHQNTIASDDGCGNGNFISIL 358
           RSI D+ P+L W                    FLEGVLK+H +T  S       +F+SIL
Sbjct: 215 RSIEDFFPLLGWVDWLTGLRGKVAEAAKGVDTFLEGVLKEHLSTTGSK----YNDFVSIL 270

Query: 359 LDVQKQNTDNSFSIDKDCIKAVILDMFFAGTDTTSTTLEWTIAALIKSPDAMFKLQKEVR 538
           L++Q+   D   S+D +CIK++I DM  AGT+T ST LEWT+AALIK+PDAMFKLQ EVR
Sbjct: 271 LEIQE--ADAGSSMDNECIKSLIWDMLGAGTETISTALEWTLAALIKNPDAMFKLQNEVR 328

Query: 539 ERGKGKSKISEGDLVNMHYLKAV 607
           E GKGKSKISE DLV M+YL+AV
Sbjct: 329 EIGKGKSKISEADLVKMNYLQAV 351


>sp|C0SJS4.1|C71AJ_APIGR RecName: Full=Psoralen synthase; AltName: Full=Cytochrome P450
           CYP71AJ2 gi|140083755|gb|ABO84855.1| cytochrome P450
           [Apium graveolens]
          Length = 476

 Score =  179 bits (453), Expect = 4e-43
 Identities = 99/203 (48%), Positives = 132/203 (65%), Gaps = 1/203 (0%)
 Frame = +2

Query: 2   VDLLIQNLQYSDQNIINLMDMLSELANNVVCRVSLGRKFSDKGNSYNKLLAEVTNLLGL- 178
           VDLL+QN++ S   +INL ++L E+  NVVC+VS+G    DK +SY  L+ E+  +LG  
Sbjct: 146 VDLLVQNIENSCSKVINLTELLIEVTGNVVCKVSVGS--GDKVDSYKILILEIMEMLGYS 203

Query: 179 RSIGDYIPVLCWXXXXXXXXXXXXXXXXXXXXFLEGVLKDHQNTIASDDGCGNGNFISIL 358
           RSI D+ P+  W                    FLEGVLK+H    AS++   + +F+SIL
Sbjct: 204 RSIEDFFPMFGWVDWLTGLRGKVAKAAKGVDDFLEGVLKEHLTARASNNASADNDFVSIL 263

Query: 359 LDVQKQNTDNSFSIDKDCIKAVILDMFFAGTDTTSTTLEWTIAALIKSPDAMFKLQKEVR 538
           L++Q+   D   ++D +CIK++I DM  AGT+T ST LEWT+AALIK+PDAM KLQ EVR
Sbjct: 264 LEIQE--ADAGSTMDNECIKSLIWDMLGAGTETISTALEWTLAALIKNPDAMLKLQNEVR 321

Query: 539 ERGKGKSKISEGDLVNMHYLKAV 607
           E GKGKSKISE DL  M YL+AV
Sbjct: 322 EIGKGKSKISEADLGKMTYLQAV 344


>sp|C0SJS2.1|C71AJ_PASSA RecName: Full=Psoralen synthase; AltName: Full=Cytochrome P450
           CYP71AJ3 gi|140083734|gb|ABO84853.1| cytochrome P450
           [Pastinaca sativa]
          Length = 473

 Score =  178 bits (451), Expect = 8e-43
 Identities = 103/203 (50%), Positives = 130/203 (64%), Gaps = 1/203 (0%)
 Frame = +2

Query: 2   VDLLIQNLQYSDQNIINLMDMLSELANNVVCRVSLGRKFSDKGNSYNKLLAEVTNLLGL- 178
           V LL+ N++ S   +INL +ML EL  NVVCR +LG  ++   +SY  LL ++ ++LG  
Sbjct: 145 VALLLDNIENSKSKVINLSEMLIELTGNVVCRAALGSGYNV--DSYKSLLLQIMDMLGYS 202

Query: 179 RSIGDYIPVLCWXXXXXXXXXXXXXXXXXXXXFLEGVLKDHQNTIASDDGCGNGNFISIL 358
           RSI D+ P L W                    FLEGVLK+H N   S     N +F+SIL
Sbjct: 203 RSIEDFFPSLGWVDWITGLKGKVEKAANGVDAFLEGVLKNHTNPSTSS---ANKDFVSIL 259

Query: 359 LDVQKQNTDNSFSIDKDCIKAVILDMFFAGTDTTSTTLEWTIAALIKSPDAMFKLQKEVR 538
           L++Q+   D   S+DK+CIK++I DM  AGT+T +T LEWTI ALIKSPDAM KLQKEVR
Sbjct: 260 LEIQE--ADAGSSMDKECIKSLIWDMLGAGTETIATALEWTIGALIKSPDAMSKLQKEVR 317

Query: 539 ERGKGKSKISEGDLVNMHYLKAV 607
           E GKGKS+I EGDLV M YLKAV
Sbjct: 318 EIGKGKSRIEEGDLVKMDYLKAV 340


>gb|ABC69416.1| CYP71AU1 [Nicotiana tabacum]
          Length = 494

 Score =  153 bits (386), Expect = 3e-35
 Identities = 80/201 (39%), Positives = 121/201 (60%), Gaps = 2/201 (0%)
 Frame = +2

Query: 8   LLIQNLQYSDQNIINLMDMLSELANNVVCRVSLGRKFSD--KGNSYNKLLAEVTNLLGLR 181
           LL+Q ++ S  + ++L ++   + N++VCRV+LGRK+ D  +G  +  LL E   LLG+ 
Sbjct: 152 LLLQRIRESPNSEVDLTELFVSMTNDIVCRVALGRKYCDGEEGRKFKSLLLEFVELLGVF 211

Query: 182 SIGDYIPVLCWXXXXXXXXXXXXXXXXXXXXFLEGVLKDHQNTIASDDGCGNGNFISILL 361
           +IGDY+P L W                    FLE V+++H     SD      +F+ ILL
Sbjct: 212 NIGDYMPWLAWMNRFNGLNAKVDKVAKEFDAFLEDVIEEHGGNKKSDTEAEGADFVDILL 271

Query: 362 DVQKQNTDNSFSIDKDCIKAVILDMFFAGTDTTSTTLEWTIAALIKSPDAMFKLQKEVRE 541
            V K+N    F ++ D IKA+I+DMF AGTDTTST LEWT+  L+++P  + KL+ EVR+
Sbjct: 272 QVHKENKAG-FQVEMDAIKAIIMDMFAAGTDTTSTLLEWTMNELLRNPKTLNKLRDEVRQ 330

Query: 542 RGKGKSKISEGDLVNMHYLKA 604
             +GK++++E DL  M YL+A
Sbjct: 331 VTQGKTEVTEDDLEKMPYLRA 351