BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00005532
         (1328 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABO61376.1| serine hydroxymethyltransferase [Populus tremuloi...   473   e-158
gb|ACJ11726.1| serine hydroxymethyltransferase [Gossypium hirsutum]   473   e-157
ref|NP_001238321.1| serine hydroxymethyltransferase 2 [Glycine m...   478   e-157
gb|AFK83582.1| serine hydroxymethyltransferase [Glycine max] gi|...   478   e-157
ref|XP_002298678.1| serine hydroxymethyltransferase 1 [Populus t...   468   e-157

>gb|ABO61376.1| serine hydroxymethyltransferase [Populus tremuloides]
          Length = 471

 Score =  473 bits (1218), Expect(3) = e-158
 Identities = 239/294 (81%), Positives = 245/294 (83%)
 Frame = -1

Query: 1328 GGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYIDYDXXXXXXXXXXXXXXXXXXXX 1149
            GGHLTHGYYTSGGKKISATSIYFESLPYKVNS TGYI                       
Sbjct: 132  GGHLTHGYYTSGGKKISATSIYFESLPYKVNSQTGYI----------------------- 168

Query: 1148 XXTGYIDYDRLEEKALDFRPKLIICGGSAYPRDWEYKRFREIADKCGALLLCDMAHISGL 969
                  DYDRLEEKALDFRPKLIICGGSAYPRDW+YKRFR +ADKCGALLLCDMAHISGL
Sbjct: 169  ------DYDRLEEKALDFRPKLIICGGSAYPRDWDYKRFRSVADKCGALLLCDMAHISGL 222

Query: 968  VAAQEAADPFEYCDIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXPENAEYDFEDKINFA 789
            VAAQEAA+PFEYCDIVTTTTHKSLRGPRAGMIFYR          PENA YDFEDK+NFA
Sbjct: 223  VAAQEAANPFEYCDIVTTTTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKVNFA 282

Query: 788  VFPSLQGGPHNHQIGALAVALKQAATPAFKAYAKQVRANAVALGKYLMSKDYKLVTGGTE 609
            VFPSLQGGPHNHQIGALAVALKQ  TP FKAYAKQV+ANAVALGKYLM + YKLVT GTE
Sbjct: 283  VFPSLQGGPHNHQIGALAVALKQVQTPGFKAYAKQVKANAVALGKYLMGQGYKLVTEGTE 342

Query: 608  NHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGTPAMT 447
            NHLVLWDLRPLGLTGNKVEKLCDL NITVNKNAVFGDSSALAPGGVRIGTPAMT
Sbjct: 343  NHLVLWDLRPLGLTGNKVEKLCDLANITVNKNAVFGDSSALAPGGVRIGTPAMT 396



 Score = 90.5 bits (223), Expect(3) = e-158
 Identities = 44/45 (97%), Positives = 44/45 (97%)
 Frame = -2

Query: 388 SRGLVEKDFEQIGEFLHRAVTITLSIQKEYGKLLKDFNKGLTNNK 254
           SRGLVEKDFEQIGEFLHRAVTITLSIQKEYGKLLKDFNKGL NNK
Sbjct: 397 SRGLVEKDFEQIGEFLHRAVTITLSIQKEYGKLLKDFNKGLVNNK 441



 Score = 44.7 bits (104), Expect(3) = e-158
 Identities = 19/23 (82%), Positives = 21/23 (91%)
 Frame = -1

Query: 164 DVEKFSSSFDMPGFSMADMKYKD 96
           DVEKFS SFDMPGF M++MKYKD
Sbjct: 449 DVEKFSGSFDMPGFLMSEMKYKD 471


>gb|ACJ11726.1| serine hydroxymethyltransferase [Gossypium hirsutum]
          Length = 471

 Score =  473 bits (1218), Expect(3) = e-157
 Identities = 238/294 (80%), Positives = 247/294 (84%)
 Frame = -1

Query: 1328 GGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYIDYDXXXXXXXXXXXXXXXXXXXX 1149
            GGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYI                       
Sbjct: 132  GGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYI----------------------- 168

Query: 1148 XXTGYIDYDRLEEKALDFRPKLIICGGSAYPRDWEYKRFREIADKCGALLLCDMAHISGL 969
                  DYD+LEEKALDFRPKLIICGGSAYPRDW+Y RFR +ADKCGALLLCDMAHISGL
Sbjct: 169  ------DYDKLEEKALDFRPKLIICGGSAYPRDWDYARFRAVADKCGALLLCDMAHISGL 222

Query: 968  VAAQEAADPFEYCDIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXPENAEYDFEDKINFA 789
            VAAQEA +PFE+CDIVTTTTHKSLRGPRAGMIFYR          PE+A YDFEDKINFA
Sbjct: 223  VAAQEANNPFEFCDIVTTTTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFA 282

Query: 788  VFPSLQGGPHNHQIGALAVALKQAATPAFKAYAKQVRANAVALGKYLMSKDYKLVTGGTE 609
            VFPSLQGGPHNHQIGALAVALKQ+ TP FKAYAKQV+ANAVALGKYLM K Y+LVTGGTE
Sbjct: 283  VFPSLQGGPHNHQIGALAVALKQSMTPGFKAYAKQVKANAVALGKYLMGKGYQLVTGGTE 342

Query: 608  NHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGTPAMT 447
            NHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGTPAMT
Sbjct: 343  NHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGTPAMT 396



 Score = 87.8 bits (216), Expect(3) = e-157
 Identities = 42/45 (93%), Positives = 44/45 (97%)
 Frame = -2

Query: 388 SRGLVEKDFEQIGEFLHRAVTITLSIQKEYGKLLKDFNKGLTNNK 254
           SRGLVEKDFEQIGEFLHRAVTITL+IQK+YGKLLKDFNKGL NNK
Sbjct: 397 SRGLVEKDFEQIGEFLHRAVTITLNIQKQYGKLLKDFNKGLDNNK 441



 Score = 45.8 bits (107), Expect(3) = e-157
 Identities = 19/23 (82%), Positives = 22/23 (95%)
 Frame = -1

Query: 164 DVEKFSSSFDMPGFSMADMKYKD 96
           DVEKF+SSFDMPGF M++MKYKD
Sbjct: 449 DVEKFASSFDMPGFKMSEMKYKD 471


>ref|NP_001238321.1| serine hydroxymethyltransferase 2 [Glycine max]
            gi|222142531|gb|ACM45952.1| serine
            hydroxymethyltransferase 2 [Glycine max]
          Length = 496

 Score =  478 bits (1230), Expect(3) = e-157
 Identities = 241/294 (81%), Positives = 245/294 (83%)
 Frame = -1

Query: 1328 GGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYIDYDXXXXXXXXXXXXXXXXXXXX 1149
            GGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYI                       
Sbjct: 157  GGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYI----------------------- 193

Query: 1148 XXTGYIDYDRLEEKALDFRPKLIICGGSAYPRDWEYKRFREIADKCGALLLCDMAHISGL 969
                  DYDRLEEKALDFRPKLIICGGSAYPRDW+YKRFRE+ADKCGALLLCDMAH SGL
Sbjct: 194  ------DYDRLEEKALDFRPKLIICGGSAYPRDWDYKRFREVADKCGALLLCDMAHTSGL 247

Query: 968  VAAQEAADPFEYCDIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXPENAEYDFEDKINFA 789
            VAAQE   PFEYCDIVTTTTHKSLRGPRAGMIFYR          PENA YDFEDKINFA
Sbjct: 248  VAAQEVNSPFEYCDIVTTTTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFA 307

Query: 788  VFPSLQGGPHNHQIGALAVALKQAATPAFKAYAKQVRANAVALGKYLMSKDYKLVTGGTE 609
            VFPSLQGGPHNHQIGALAVALKQAA+P FKAYAKQV+ANAVALGKYLM K Y LVTGGTE
Sbjct: 308  VFPSLQGGPHNHQIGALAVALKQAASPGFKAYAKQVKANAVALGKYLMGKGYSLVTGGTE 367

Query: 608  NHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGTPAMT 447
            NHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIG PAMT
Sbjct: 368  NHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMT 421



 Score = 86.3 bits (212), Expect(3) = e-157
 Identities = 41/45 (91%), Positives = 43/45 (95%)
 Frame = -2

Query: 388 SRGLVEKDFEQIGEFLHRAVTITLSIQKEYGKLLKDFNKGLTNNK 254
           SRGLVEKDFEQIGEFLHRAVT+TL IQKE+GKLLKDFNKGL NNK
Sbjct: 422 SRGLVEKDFEQIGEFLHRAVTLTLEIQKEHGKLLKDFNKGLVNNK 466



 Score = 41.2 bits (95), Expect(3) = e-157
 Identities = 17/23 (73%), Positives = 21/23 (91%)
 Frame = -1

Query: 164 DVEKFSSSFDMPGFSMADMKYKD 96
           DVEKFS+ FDMPGF +++MKYKD
Sbjct: 474 DVEKFSALFDMPGFLVSEMKYKD 496


>gb|AFK83582.1| serine hydroxymethyltransferase [Glycine max]
            gi|389548698|gb|AFK83587.1| serine
            hydroxymethyltransferase [Glycine max]
          Length = 471

 Score =  478 bits (1230), Expect(3) = e-157
 Identities = 241/294 (81%), Positives = 245/294 (83%)
 Frame = -1

Query: 1328 GGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYIDYDXXXXXXXXXXXXXXXXXXXX 1149
            GGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYI                       
Sbjct: 132  GGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYI----------------------- 168

Query: 1148 XXTGYIDYDRLEEKALDFRPKLIICGGSAYPRDWEYKRFREIADKCGALLLCDMAHISGL 969
                  DYDRLEEKALDFRPKLIICGGSAYPRDW+YKRFRE+ADKCGALLLCDMAH SGL
Sbjct: 169  ------DYDRLEEKALDFRPKLIICGGSAYPRDWDYKRFREVADKCGALLLCDMAHTSGL 222

Query: 968  VAAQEAADPFEYCDIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXPENAEYDFEDKINFA 789
            VAAQE   PFEYCDIVTTTTHKSLRGPRAGMIFYR          PENA YDFEDKINFA
Sbjct: 223  VAAQEVNSPFEYCDIVTTTTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFA 282

Query: 788  VFPSLQGGPHNHQIGALAVALKQAATPAFKAYAKQVRANAVALGKYLMSKDYKLVTGGTE 609
            VFPSLQGGPHNHQIGALAVALKQAA+P FKAYAKQV+ANAVALGKYLM K Y LVTGGTE
Sbjct: 283  VFPSLQGGPHNHQIGALAVALKQAASPGFKAYAKQVKANAVALGKYLMGKGYSLVTGGTE 342

Query: 608  NHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGTPAMT 447
            NHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIG PAMT
Sbjct: 343  NHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMT 396



 Score = 86.3 bits (212), Expect(3) = e-157
 Identities = 41/45 (91%), Positives = 43/45 (95%)
 Frame = -2

Query: 388 SRGLVEKDFEQIGEFLHRAVTITLSIQKEYGKLLKDFNKGLTNNK 254
           SRGLVEKDFEQIGEFLHRAVT+TL IQKE+GKLLKDFNKGL NNK
Sbjct: 397 SRGLVEKDFEQIGEFLHRAVTLTLEIQKEHGKLLKDFNKGLVNNK 441



 Score = 41.2 bits (95), Expect(3) = e-157
 Identities = 17/23 (73%), Positives = 21/23 (91%)
 Frame = -1

Query: 164 DVEKFSSSFDMPGFSMADMKYKD 96
           DVEKFS+ FDMPGF +++MKYKD
Sbjct: 449 DVEKFSALFDMPGFLVSEMKYKD 471


>ref|XP_002298678.1| serine hydroxymethyltransferase 1 [Populus trichocarpa]
            gi|118481215|gb|ABK92558.1| unknown [Populus trichocarpa]
            gi|222845936|gb|EEE83483.1| serine
            hydroxymethyltransferase 1 [Populus trichocarpa]
          Length = 471

 Score =  468 bits (1204), Expect(3) = e-157
 Identities = 236/294 (80%), Positives = 244/294 (82%)
 Frame = -1

Query: 1328 GGHLTHGYYTSGGKKISATSIYFESLPYKVNSTTGYIDYDXXXXXXXXXXXXXXXXXXXX 1149
            GGHLTHGYYTSGGKKISATSIYFESLPYKVNS +GYID                      
Sbjct: 132  GGHLTHGYYTSGGKKISATSIYFESLPYKVNSQSGYID---------------------- 169

Query: 1148 XXTGYIDYDRLEEKALDFRPKLIICGGSAYPRDWEYKRFREIADKCGALLLCDMAHISGL 969
                   YD+LEEKALDFRPKLIICGGSAYPRDW+YK+FR +ADKCGALLLCDMAHISGL
Sbjct: 170  -------YDKLEEKALDFRPKLIICGGSAYPRDWDYKKFRSVADKCGALLLCDMAHISGL 222

Query: 968  VAAQEAADPFEYCDIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXPENAEYDFEDKINFA 789
            VAAQEAA+PFEYCDIVTTTTHKSLRGPRAGMIFYR          PENA YDFEDKINFA
Sbjct: 223  VAAQEAANPFEYCDIVTTTTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFA 282

Query: 788  VFPSLQGGPHNHQIGALAVALKQAATPAFKAYAKQVRANAVALGKYLMSKDYKLVTGGTE 609
            VFPSLQGGPHNHQIGALAVALKQ  TP FKAYAKQV+ANAVALG YLM + YKLVT GTE
Sbjct: 283  VFPSLQGGPHNHQIGALAVALKQVQTPGFKAYAKQVKANAVALGNYLMGQGYKLVTEGTE 342

Query: 608  NHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGTPAMT 447
            NHLVLWDLRPLGLTGNKVEKLCDL NITVNKNAVFGDSSALAPGGVRIGTPAMT
Sbjct: 343  NHLVLWDLRPLGLTGNKVEKLCDLANITVNKNAVFGDSSALAPGGVRIGTPAMT 396



 Score = 90.5 bits (223), Expect(3) = e-157
 Identities = 44/45 (97%), Positives = 44/45 (97%)
 Frame = -2

Query: 388 SRGLVEKDFEQIGEFLHRAVTITLSIQKEYGKLLKDFNKGLTNNK 254
           SRGLVEKDFEQIGEFLHRAVTITLSIQKEYGKLLKDFNKGL NNK
Sbjct: 397 SRGLVEKDFEQIGEFLHRAVTITLSIQKEYGKLLKDFNKGLVNNK 441



 Score = 45.4 bits (106), Expect(3) = e-157
 Identities = 19/23 (82%), Positives = 21/23 (91%)
 Frame = -1

Query: 164 DVEKFSSSFDMPGFSMADMKYKD 96
           DVEKFS SFDMPGF M++MKYKD
Sbjct: 449 DVEKFSGSFDMPGFQMSEMKYKD 471